FastQCFastQC Report
Thu 26 May 2016
SRR938833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454161
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59241.3043832473506092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50301.1075367545870298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.7116419067247077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11880.26158124541737404No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.2450672779036509No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.2362598285629986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.2168834400135635No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.15413036346141568No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.1116344203927682No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.11141423415925189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.041.6960871
GTATCAA53000.037.037241
CTGTTCG600.004007019631.571569
GTCGGGA2600.030.9849012
TATCAAC64900.029.8643572
ATCAACG66100.029.322193
TCAACGC66850.028.993224
CAACGCA67600.028.7416525
AACGCAG67250.028.7312936
TAAGGTG1057.007811E-527.0792435
GTATGAT909.033315E-426.3415661
GGTCGGG2950.025.7165131
GTATAGG1301.0143871E-525.5310571
GGACCAT1301.0239803E-525.5001076
ACGCAGA76100.025.2033147
CCCTATA950.001242285124.9414082
CGCAGAG75800.024.8629888
GCAGAGT76850.023.9097589
GTACATG31250.023.8212051
CGGGAGT3000.023.6943384
Produced by FastQC (version 0.11.5)