##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938833_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 454161 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48187757205044 33.0 31.0 34.0 30.0 34.0 2 31.490669608354747 33.0 31.0 34.0 30.0 34.0 3 31.45663101851546 33.0 31.0 34.0 28.0 34.0 4 35.03666981532981 37.0 35.0 37.0 33.0 37.0 5 35.069561234892475 37.0 35.0 37.0 33.0 37.0 6 35.208164065166315 37.0 35.0 37.0 33.0 37.0 7 35.13704170987822 37.0 35.0 37.0 32.0 37.0 8 35.1144792265298 37.0 35.0 37.0 32.0 37.0 9 36.64036762293548 39.0 37.0 39.0 32.0 39.0 10-11 36.565548340786634 39.0 37.0 39.0 32.0 39.0 12-13 36.44371269219506 39.0 37.0 39.0 32.0 39.0 14-15 37.49843337494853 40.0 37.0 41.0 32.0 41.0 16-17 37.454377412415425 40.0 37.0 41.0 32.0 41.0 18-19 37.50327086649888 40.0 37.0 41.0 32.0 41.0 20-21 37.45655395333373 40.0 37.0 41.0 32.0 41.0 22-23 37.30921633517629 40.0 37.0 41.0 32.0 41.0 24-25 37.219895587688065 40.0 37.0 41.0 32.0 41.0 26-27 37.049148870114344 40.0 36.5 41.0 31.0 41.0 28-29 36.85399340762417 39.5 36.0 41.0 31.0 41.0 30-31 36.46327403718065 39.0 35.5 41.0 30.5 41.0 32-33 36.011351701268936 39.0 35.0 41.0 30.0 41.0 34-35 35.5405483958332 38.0 35.0 41.0 28.0 41.0 36-37 35.13813493452763 38.0 35.0 40.0 26.0 41.0 38-39 34.82048106288299 38.0 35.0 40.0 24.5 41.0 40-41 34.43711260984541 38.0 35.0 40.0 23.5 41.0 42-43 33.92959545183316 38.0 34.0 40.0 19.5 41.0 44-45 33.438561435261946 38.0 33.0 40.0 9.0 41.0 46-47 32.77519976396036 38.0 33.0 40.0 2.0 41.0 48-49 32.155482306935205 38.0 32.5 40.0 2.0 41.0 50-51 30.875218259603972 36.5 30.5 39.0 2.0 40.5 52-53 30.853746799042632 37.0 30.5 39.5 2.0 40.5 54-55 31.240617314124286 37.0 31.0 40.0 2.0 41.0 56-57 31.085529140547074 37.0 31.0 40.0 2.0 41.0 58-59 30.822905313314003 37.0 31.0 40.0 2.0 41.0 60-61 30.508245974445188 36.0 30.0 40.0 2.0 41.0 62-63 30.121581333491868 35.5 29.0 39.5 2.0 41.0 64-65 29.711442638183378 35.0 28.5 39.0 2.0 41.0 66-67 29.303731496099402 35.0 28.0 39.0 2.0 41.0 68-69 28.863955733759614 35.0 26.5 38.5 2.0 40.0 70-71 28.44188624738804 35.0 26.0 37.5 2.0 40.0 72-73 28.00307269888872 35.0 26.0 37.0 2.0 39.5 74-75 27.52953468043271 34.5 24.5 36.5 2.0 39.0 76-77 27.073880187862894 34.0 23.5 36.0 2.0 39.0 78-79 26.622207763326223 34.0 21.0 35.5 2.0 37.5 80-81 26.15241621363349 34.0 19.0 35.0 2.0 37.0 82-83 25.65564964847268 34.0 14.5 35.0 2.0 37.0 84-85 25.206394868780016 33.0 6.0 35.0 2.0 36.0 86-87 24.794228258260837 33.0 2.0 35.0 2.0 36.0 88-89 24.383211019880612 33.0 2.0 35.0 2.0 35.5 90-91 23.92909452815191 33.0 2.0 35.0 2.0 35.0 92-93 23.442009551678808 32.0 2.0 35.0 2.0 35.0 94-95 22.920752772695145 32.0 2.0 35.0 2.0 35.0 96-97 22.348812425549532 31.0 2.0 35.0 2.0 35.0 98-99 21.698272198625595 31.0 2.0 35.0 2.0 35.0 100-101 19.935336807872098 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17884122448758788 1101 2 0.47478573782276 1101 3 0.474333461099711 1101 4 0.20692458647261702 1101 5 0.2795120826712676 1101 6 0.26668088344980845 1101 7 0.35918626068146864 1101 8 0.5140640143028676 1101 9 0.2747827038832895 1101 10-11 0.4540558408156201 1101 12-13 0.673488518038674 1101 14-15 0.6946980803362948 1101 16-17 0.8153708361582304 1101 18-19 0.5763973693579771 1101 20-21 0.5395217999274209 1101 22-23 0.5651921743457606 1101 24-25 0.38689233012157587 1101 26-27 0.3224488006774777 1101 28-29 0.3169311030812807 1101 30-31 0.4874584841143772 1101 32-33 0.6547779148813575 1101 34-35 0.46872054215246095 1101 36-37 0.49476705616307015 1101 38-39 0.6467280636142334 1101 40-41 0.5692545619094034 1101 42-43 0.7609220344054819 1101 44-45 0.8139835721559194 1101 46-47 1.408582894511511 1101 48-49 1.5919263399129306 1101 50-51 1.180530329338815 1101 52-53 1.0896180544697849 1101 54-55 1.1741340834941134 1101 56-57 0.8700142978298118 1101 58-59 1.1169492225755313 1101 60-61 1.035865713471221 1101 62-63 0.8612504903158964 1101 64-65 0.9069307658475658 1101 66-67 0.9027449827435028 1101 68-69 0.9204434597568181 1101 70-71 1.0413713088004144 1101 72-73 0.46191746884773366 1101 74-75 0.6789537550970444 1101 76-77 0.34779513522475725 1101 78-79 0.20043311287201604 1101 80-81 0.21498225038861563 1101 82-83 0.52802580326005 1101 84-85 0.2848053247118685 1101 86-87 0.38486553757812203 1101 88-89 0.2604921303056713 1101 90-91 0.36788867159800986 1101 92-93 0.15046341162741328 1101 94-95 0.26631442502386093 1101 96-97 0.1250685811639407 1101 98-99 0.2702107271424232 1101 100-101 0.10879663485641089 1102 1 0.09511573429151099 1102 2 -0.0660966151184148 1102 3 0.024333461099711684 1102 4 0.12329713549222276 1102 5 0.11657090620067834 1102 6 0.008445589332161774 1102 7 0.1705588097010775 1102 8 -0.006230103344194049 1102 9 -0.021197688273574045 1102 10-11 0.01180093885483302 1102 12-13 -0.00126638392210765 1102 14-15 -0.0414293706440958 1102 16-17 0.1485080910602008 1102 18-19 0.2271816830834723 1102 20-21 0.23143356463330633 1102 22-23 0.11484903709085614 1102 24-25 0.1474315458078479 1102 26-27 0.23024291832453514 1102 28-29 0.3840879658263745 1102 30-31 0.2581937782320196 1102 32-33 0.06561124821468667 1102 34-35 -0.0880441637298901 1102 36-37 0.15207097773169664 1102 38-39 0.026433945967170303 1102 40-41 0.02160750308587467 1102 42-43 0.14842203440547763 1102 44-45 0.4532482780382736 1102 46-47 0.3797593650997442 1102 48-49 -0.20224032675373138 1102 50-51 -0.20638143536706366 1102 52-53 -0.05258782788315486 1102 54-55 -0.5688070929764777 1102 56-57 -0.44518178060155833 1102 58-59 -0.49344293428721286 1102 60-61 -0.6542323257444629 1102 62-63 -0.6193867645860607 1102 64-65 -0.5028241361132153 1102 66-67 -0.4607354094133562 1102 68-69 -0.6709290892627884 1102 70-71 -0.8258345735525268 1102 72-73 -0.9838668448777597 1102 74-75 -0.767663891961778 1102 76-77 -0.6183813353634804 1102 78-79 -0.957900220461319 1102 80-81 -0.7863412790231479 1102 82-83 -0.6052585104654398 1102 84-85 -0.5950476164646012 1102 86-87 -0.5415560310493248 1102 88-89 -0.574507869694326 1102 90-91 -0.7816701519314044 1102 92-93 -0.8563503138627837 1102 94-95 -1.3127542024271186 1102 96-97 -1.2595882815811557 1102 98-99 -1.838661821877185 1102 100-101 -1.3059582671043728 1103 1 -0.07295385974328639 1103 2 0.09716215317763499 1103 3 -0.028497248648978513 1103 4 0.09939213687305681 1103 5 0.20059741821393828 1103 6 0.15573086964146654 1103 7 0.1720832504632952 1103 8 0.15073206180355925 1103 9 0.31534470333095754 1103 10-11 0.008011930293655212 1103 12-13 0.2029865848710486 1103 14-15 0.20148142747796527 1103 16-17 0.28078094386329155 1103 18-19 0.5480902718710965 1103 20-21 0.5759240368785399 1103 22-23 0.41065958666279556 1103 24-25 0.12883171150792805 1103 26-27 0.12500679570647577 1103 28-29 0.24730254743366942 1103 30-31 0.34676875751951286 1103 32-33 -0.03206618895736568 1103 34-35 0.10690750707927776 1103 36-37 -0.03990913825835918 1103 38-39 0.13122820169763116 1103 40-41 0.4066613556127976 1103 42-43 0.2867263702243221 1103 44-45 0.08260757105125549 1103 46-47 -0.16045880667601153 1103 48-49 -0.2407683576844164 1103 50-51 -0.27595613848775 1103 52-53 -0.13544063097622328 1103 54-55 -0.017525679002435623 1103 56-57 0.06743904237551845 1103 58-59 -0.04115627314388348 1103 60-61 -0.12572638815816362 1103 62-63 -0.15695925837231073 1103 64-65 -0.31788627364428734 1103 66-67 -0.006137232114269864 1103 68-69 -0.007062753996290638 1103 70-71 -0.34141314300847725 1103 72-73 -0.44995079958640005 1103 74-75 -0.5534578715254348 1103 76-77 -0.4996572260014247 1103 78-79 -0.5873672185281507 1103 80-81 -0.3810720163885186 1103 82-83 -0.3566227744809076 1103 84-85 -0.053067500474544005 1103 86-87 -0.5668225320159124 1103 88-89 -0.6000091124449476 1103 90-91 -0.6741749848504881 1103 92-93 -1.1383552848652698 1103 94-95 -0.9503902145784586 1103 96-97 -1.0122726229233265 1103 98-99 -0.8689600820263159 1103 100-101 -1.1302775159306648 1104 1 0.05838821569786745 1104 2 0.397081207734864 1104 3 0.13224421160681032 1104 4 0.1880435857964855 1104 5 0.15094548361785343 1104 6 0.2163428171888171 1104 7 0.16709701118856657 1104 8 0.17459477833261872 1104 9 0.29298757203744685 1104 10-11 0.2976832917960053 1104 12-13 0.21969372425909484 1104 14-15 0.38454764085015114 1104 16-17 0.22318016678703856 1104 18-19 -0.1278284589043608 1104 20-21 -0.12137238533289008 1104 22-23 -0.07790282227357892 1104 24-25 0.09746535243394305 1104 26-27 0.36517192440972934 1104 28-29 0.24798248915295318 1104 30-31 0.23251426504743478 1104 32-33 0.2320345071734451 1104 34-35 0.019988290631161476 1104 36-37 0.3558978878060728 1104 38-39 0.37568343886778166 1104 40-41 0.2752383347288756 1104 42-43 0.20400012771177245 1104 44-45 0.5660542280044751 1104 46-47 0.7376227863303058 1104 48-49 1.0733242439021211 1104 50-51 1.1984731961407107 1104 52-53 0.8210835717111671 1104 54-55 0.8920177887003327 1104 56-57 0.7968060490130426 1104 58-59 0.8542379311624124 1104 60-61 0.9341246722271386 1104 62-63 0.9034665146566709 1104 64-65 0.9347282641572363 1104 66-67 0.7624559360903582 1104 68-69 0.8395780101286903 1104 70-71 0.9618327692466622 1104 72-73 1.027586840044485 1104 74-75 0.4703077104722979 1104 76-77 0.3730317816074482 1104 78-79 0.2926068789301617 1104 80-81 0.12273241942174806 1104 82-83 0.2997719154980487 1104 84-85 0.19767719759895286 1104 86-87 -0.21198199453817068 1104 88-89 -0.0886119941027097 1104 90-91 -0.3071197800585175 1104 92-93 -0.013456466668735345 1104 94-95 -0.23879882717356793 1104 96-97 -0.22955447495505865 1104 98-99 0.0910609637888058 1104 100-101 -0.19584332457563747 1105 1 -0.26280529713475786 1105 2 -0.11831794200533352 1105 3 -0.1048365630743433 1105 4 0.0721878150517199 1105 5 -0.024208035512991444 1105 6 0.11498869972700021 1105 7 0.20124911321974537 1105 8 0.32540567425451883 1105 9 -0.03770050857978191 1105 10-11 -0.03724821505330311 1105 12-13 0.10182252287349058 1105 14-15 0.08468935081709361 1105 16-17 0.13144658152487665 1105 18-19 -0.2016042422455655 1105 20-21 -0.04188096665866681 1105 22-23 0.09589657939545049 1105 24-25 0.017281129476927504 1105 26-27 -0.08093892427551452 1105 28-29 0.11692438806650784 1105 30-31 0.12013038849256219 1105 32-33 -0.009588859225551971 1105 34-35 0.0058881489211941584 1105 36-37 -0.02986026051702595 1105 38-39 -0.340345532947687 1105 40-41 -0.5710220966992452 1105 42-43 -0.9384971168166558 1105 44-45 -0.5184600217199815 1105 46-47 -0.31025943694162095 1105 48-49 -0.5649531927220366 1105 50-51 -0.3908670652354509 1105 52-53 -0.42893217384071747 1105 54-55 -0.9843402651494522 1105 56-57 -0.8929121056082749 1105 58-59 -0.7097362461325005 1105 60-61 -0.6317074801898066 1105 62-63 -0.8330316746048645 1105 64-65 -0.6142718932982838 1105 66-67 -0.6557931585404084 1105 68-69 -0.7029113616237872 1105 70-71 -0.5559124677238962 1105 72-73 -0.2673667105774662 1105 74-75 -0.814985944070294 1105 76-77 -0.9220477334295545 1105 78-79 -0.8037637713611296 1105 80-81 -0.8334162185880167 1105 82-83 -0.7183984513733108 1105 84-85 -0.8907419489921331 1105 86-87 -1.0448114702112932 1105 88-89 -1.0133797472124542 1105 90-91 -0.9499161900726882 1105 92-93 -1.1696917052138431 1105 94-95 -0.726517296705925 1105 96-97 -0.8734373280490573 1105 98-99 -0.9249443896988332 1105 100-101 -0.8552276734970654 1106 1 0.10211983825868032 1106 2 0.21004565574341783 1106 3 0.49054413141434594 1106 4 0.4932560958205414 1106 5 0.1607022331500616 1106 6 0.2822075592364044 1106 7 0.24555196975579463 1106 8 0.24337317982953977 1106 9 -0.08669700427904559 1106 10-11 0.26396236156345054 1106 12-13 0.2581727405649019 1106 14-15 0.3967101642396216 1106 16-17 0.33860385029412754 1106 18-19 0.4540079484733468 1106 20-21 0.30763055505738635 1106 22-23 0.2696780384588422 1106 24-25 0.030878878229188444 1106 26-27 -0.3201127588300494 1106 28-29 -0.10394212993285379 1106 30-31 0.14346053609795462 1106 32-33 0.15734859431591985 1106 34-35 -0.1723533292565662 1106 36-37 -0.3349468061260268 1106 38-39 -0.40098055471682414 1106 40-41 -0.6012652739319151 1106 42-43 -0.42927974398028823 1106 44-45 -0.51178592168813 1106 46-47 -0.7089957648592176 1106 48-49 -0.7826233636894386 1106 50-51 -0.5498561275695266 1106 52-53 -0.6129982245999805 1106 54-55 -0.6601340423608804 1106 56-57 -0.6756879365523147 1106 58-59 -0.8123485430423436 1106 60-61 -0.36070291397976106 1106 62-63 -0.6089900477597965 1106 64-65 -0.3332482127206049 1106 66-67 -0.6077258334899653 1106 68-69 -0.5313121261984968 1106 70-71 -0.7123051617878211 1106 72-73 -0.8902827135508069 1106 74-75 -0.9589224418933817 1106 76-77 -0.8233060959653926 1106 78-79 -0.7445623271644628 1106 80-81 -0.8265191176004372 1106 82-83 -0.403876841518791 1106 84-85 -0.6952391349324536 1106 86-87 -0.6771784660698472 1106 88-89 -0.6252465837846124 1106 90-91 -0.5584964173212796 1106 92-93 -0.6207005190611383 1106 94-95 -0.4526721230837545 1106 96-97 -0.37753629799702537 1106 98-99 -0.2468834361042731 1106 100-101 -0.02177222059274797 1107 1 0.08898653261083922 1107 2 0.08258510757065807 1107 3 0.19479039387281816 1107 4 -0.04460727627247962 1107 5 -0.2019742618665461 1107 6 -0.22793571318884887 1107 7 -0.18449371130732573 1107 8 0.11634867816841421 1107 9 -1.210076013080652E-4 1107 10-11 0.1052725775102914 1107 12-13 0.2850518933888182 1107 14-15 -0.24892937064409892 1107 16-17 0.021296956606413175 1107 18-19 -0.1215630648156889 1107 20-21 -0.07366097318182341 1107 22-23 0.012955654737915268 1107 24-25 -0.19908893738542588 1107 26-27 -0.16507745982671906 1107 28-29 -0.25591991232487743 1107 30-31 -0.34480254529739085 1107 32-33 -0.18336984422229108 1107 34-35 -0.09220382759544066 1107 36-37 0.08059513739556223 1107 38-39 -0.04996311285636068 1107 40-41 0.43401296527074607 1107 42-43 0.1709798075147262 1107 44-45 -0.04013932700374312 1107 46-47 -0.008762168513705149 1107 48-49 0.32678278248996406 1107 50-51 0.2597057495068853 1107 52-53 0.08068948304121548 1107 54-55 0.0772984742504157 1107 56-57 -0.34326476379483495 1107 58-59 -0.07378957294267607 1107 60-61 -0.09775648540832904 1107 62-63 -0.23241197467009655 1107 64-65 -0.27175008149136914 1107 66-67 -0.14119759428730916 1107 68-69 -0.09601312287623642 1107 70-71 -0.04712659035925171 1107 72-73 -0.3961226221886811 1107 74-75 -0.283756328936569 1107 76-77 -0.21378574712818477 1107 78-79 -0.520439611217622 1107 80-81 -0.4719925395273492 1107 82-83 -0.7355421239108182 1107 84-85 -0.9001342761284654 1107 86-87 -0.4412461570997479 1107 88-89 -0.720403352887601 1107 90-91 -0.36930933260367027 1107 92-93 -0.599842960921606 1107 94-95 -0.22694537889770672 1107 96-97 -0.5247694790601471 1107 98-99 -0.4209438596923114 1107 100-101 -0.24720826710437294 1108 1 0.07505488236453317 1108 2 0.24722488589578262 1108 3 0.11974421160681104 1108 4 0.01088841338269475 1108 5 0.24376157557187383 1108 6 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-1.0174349810536754 2316 84-85 -0.9635182046998985 2316 86-87 -0.9190854428140334 2316 88-89 -1.2010372814590298 2316 90-91 -0.953728975460816 2316 92-93 -1.3164679609216066 2316 94-95 -1.6866953788977064 2316 96-97 -1.3781176933458639 2316 98-99 -1.7347206454065933 2316 100-101 -2.370958267104374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4431.0 3 904.0 4 704.0 5 703.0 6 814.0 7 931.0 8 1128.0 9 1412.0 10 1839.0 11 2702.0 12 4213.0 13 5848.0 14 6461.0 15 7224.0 16 9083.0 17 11709.0 18 9385.0 19 5276.0 20 4701.0 21 4630.0 22 4755.0 23 4964.0 24 5310.0 25 5685.0 26 6627.0 27 7423.0 28 8345.0 29 9643.0 30 11401.0 31 13380.0 32 16127.0 33 19879.0 34 26315.0 35 36948.0 36 50384.0 37 69126.0 38 63183.0 39 10564.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.86422557326147 19.326376422954276 14.918470949900541 19.89092705388371 2 16.464466820492817 22.241101553908575 39.13707496710809 22.15735665849052 3 19.493867261356474 27.402962577733508 31.47970224374937 21.62346791716065 4 12.798642334479416 18.25949925063916 36.79427840959182 32.147580005289605 5 12.398758426693826 38.317923891909054 33.125669616285066 16.157648065112053 6 28.27777765540575 36.67577848802504 18.06365352464487 16.982790331924342 7 24.630431191144886 36.62360262128972 21.524092736384162 17.22187345118123 8 26.00828701898613 32.85443967900468 20.656529276615053 20.48074402539414 9 27.197428797381217 16.39538188042322 21.650794909582753 34.75639441261282 10-11 23.97361890821138 27.764248730343873 26.670213222927497 21.591919138517255 12-13 26.4235692713026 26.516751955624773 27.681535509652004 19.37814326342062 14-15 23.327440646323126 26.70719954180228 28.691327837163577 21.27403197471101 16-17 19.59375588566041 30.01827239414623 29.228781609094124 21.159190111099242 18-19 19.23544190585496 27.03456449354938 34.26732327565792 19.462670324937744 20-21 20.820629521602356 26.16680471964964 34.349307825841755 18.663257932906248 22-23 20.474302465592856 26.61134937255779 33.84750802273356 19.066840139115797 24-25 19.677763050177777 26.813781067064202 34.415646661003336 19.092809221754685 26-27 19.53499288198194 27.016294311315225 34.3157887777724 19.132924028930447 28-29 19.29476261091635 27.100938010237247 34.45584609316643 19.148453285679974 30-31 19.435855123647663 27.334456217964465 34.0733062269424 19.156382431445472 32-33 19.136398082549082 27.553113214197595 33.76346004723133 19.547028656021993 34-35 19.53274319867413 27.39952455036001 33.77028472874748 19.297447522218384 36-37 19.632827414727164 27.140088795683653 33.447259036077945 19.779824753511235 38-39 19.47731253759864 27.025018578152093 33.710609894193 19.787058990056266 40-41 19.61432244969255 27.105660569262984 33.53070849428106 19.749308486763407 42-43 19.507215924799556 27.29178877522809 33.04263201548244 20.15836328448991 44-45 19.48882884536901 27.545597621657254 32.646249166352376 20.31932436662136 46-47 19.707134720240663 27.599898249223042 31.840914872203236 20.852052158333056 48-49 19.79586667064889 27.698041076661166 31.3267338999156 21.17935835277434 50-51 19.958014562177933 27.957921654465157 30.68255093610051 21.401512847256402 52-53 19.9406454010694 28.102400509701813 30.55699966152614 21.399954427702646 54-55 19.60409136557289 27.932152700664048 30.707362069041793 21.756393864721264 56-57 19.845238279530893 28.171255188763745 30.255606739808794 21.727899791896572 58-59 19.787541705636148 27.615930060560235 30.576284002945098 22.020244230858523 60-61 19.841803139180968 27.44119837934975 30.846619556111587 21.870378925357695 62-63 19.897444063665628 27.354368690471055 30.825722364691742 21.922464881171575 64-65 19.69809943137819 27.385888443923267 30.794964267539882 22.12104785715866 66-67 19.630717490029483 27.65713590326524 30.238130797696687 22.47401580900859 68-69 19.59293725334434 27.482488623552154 30.307100474530362 22.617473648573146 70-71 19.51060842589038 27.41982415359952 30.08960488218822 22.979962538321878 72-73 19.76193373527297 27.2299352840312 29.753083688257092 23.25504729243874 74-75 19.39104319113279 27.25947312238317 29.758021249882464 23.591462436601567 76-77 19.449630373519614 26.99434610841492 29.865642654586154 23.69038086347931 78-79 19.448529330464453 27.020802328127036 29.77295896366503 23.757709377743478 80-81 19.451091139083744 27.080882287897957 29.6137889550364 23.8542376179819 82-83 19.496731541865127 26.822258028485518 29.44753049215426 24.23347993749509 84-85 19.421212050838854 26.45960438763244 29.493104374914985 24.626079186613715 86-87 19.56571989785028 26.151258124917742 29.65184374941244 24.631178227819543 88-89 19.638584735254526 25.816624394258326 29.826437934776234 24.718352935710914 90-91 19.689300284063254 25.79179313823527 29.944669610541315 24.574236967160164 92-93 19.84365078838321 25.535341735969613 29.90800905307732 24.71299842256986 94-95 19.783509317285514 25.0806170592888 30.091430531049323 25.044443092376362 96-97 19.966568799463687 24.68289034964478 30.10190874233638 25.248632108555157 98-99 20.044689771130848 24.530480857512636 30.14059578986958 25.28423358148693 100-101 20.54925425959619 23.996305861403442 30.645312923401924 24.809126955598444 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1262.0 1 833.0 2 513.0 3 588.5 4 517.0 5 514.0 6 788.5 7 1379.0 8 1959.0 9 2176.0 10 1909.5 11 1621.0 12 1638.0 13 1749.5 14 1794.5 15 1858.0 16 1940.0 17 2008.5 18 2052.5 19 2146.5 20 2392.0 21 2680.0 22 2982.0 23 3347.5 24 3757.5 25 4175.5 26 4906.0 27 5765.5 28 6309.0 29 6811.0 30 7378.0 31 7935.0 32 8540.0 33 9143.5 34 9791.0 35 10236.0 36 10656.5 37 11369.0 38 11498.0 39 11254.0 40 11326.5 41 11632.0 42 12072.0 43 12648.5 44 13172.0 45 13559.0 46 13947.0 47 14268.0 48 15094.0 49 15492.0 50 14836.5 51 13957.5 52 13085.5 53 12730.0 54 12296.0 55 11327.0 56 10419.0 57 9369.0 58 8523.5 59 7922.5 60 7035.0 61 6120.5 62 5246.5 63 4146.5 64 3047.5 65 2422.5 66 1972.0 67 1514.0 68 1191.0 69 921.0 70 716.5 71 563.0 72 454.0 73 370.0 74 308.0 75 239.5 76 173.0 77 125.5 78 93.0 79 70.5 80 51.0 81 42.5 82 32.0 83 20.5 84 12.5 85 11.0 86 8.0 87 6.0 88 6.0 89 3.5 90 2.0 91 1.0 92 1.5 93 2.5 94 4.0 95 3.0 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15479092216196458 2 0.08851486587355585 3 0.08080834770048506 4 0.09710212898069187 5 0.11956112479935528 6 0.03787203216480499 7 0.0409546394340333 8 0.043596874236229 9 0.0462391090384247 10-11 0.04392715358650347 12-13 0.04711985397248993 14-15 0.045908829688150236 16-17 0.04348678111947085 18-19 0.046789574622215474 20-21 0.05042264747523455 22-23 0.06484484577055274 24-25 0.0802578821166943 26-27 0.084331327436746 28-29 0.07222108459334906 30-31 0.05956037616616135 32-33 0.04954190254116932 34-35 0.2920770387593827 36-37 0.46503332518644275 38-39 0.44411563300239343 40-41 0.41659235381285487 42-43 0.4482991714392033 44-45 0.4583176450641953 46-47 0.4571066207798556 48-49 0.4739508676438532 50-51 0.4622809972674889 52-53 0.4694370498567689 54-55 0.459528669348535 56-57 0.43640911482932265 58-59 0.4148308639447244 60-61 0.38532590865353916 62-63 0.4442257261191516 64-65 0.482428037634231 66-67 0.48518036555318483 68-69 0.49332725619328827 70-71 0.4915657663251578 72-73 0.48132710646664956 74-75 0.475271985044951 76-77 0.4586479244144698 78-79 0.42980352782383335 80-81 0.43442743872767586 82-83 0.4484092645559615 84-85 0.44620740222079835 86-87 0.45787727259716265 88-89 0.46954714297352707 90-91 0.47318021582654607 92-93 0.47560226439522546 94-95 0.4796757097152772 96-97 0.4817674789336821 98-99 0.47472151946116026 100-101 0.460959879866391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 454161.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.74265686596614 #Duplication Level Percentage of deduplicated Percentage of total 1 87.27913868877138 60.87079021128052 2 7.949620394879525 11.088552948295382 3 1.9553488984818999 4.09113681840204 4 0.8670192889211509 2.4187291505360715 5 0.471464129103728 1.644058049034643 6 0.2950254489683501 1.2345515192476348 7 0.21858440836867726 1.0671260172374815 8 0.15136990411208381 0.8445551425858608 9 0.11498258617553062 0.7217271947881273 >10 0.6452542600783303 8.125911568271459 >50 0.03542729173063146 1.7033032319159864 >100 0.01360154938822986 1.720510532109968 >500 0.0012652604082074282 0.5951964887335162 >1k 0.0012652604082074282 1.4573269105165336 >5k 6.326302041037141E-4 2.4165242170448242 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5924 1.3043832473506092 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5030 1.1075367545870298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.7116419067247077 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1188 0.26158124541737404 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1113 0.2450672779036509 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1073 0.2362598285629986 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 985 0.2168834400135635 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 700 0.15413036346141568 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 507 0.1116344203927682 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 506 0.11141423415925189 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 1.1009311675815405E-4 0.0 0.0 12-13 0.0 0.0 2.201862335163081E-4 0.0 0.0 14-15 0.0 0.0 2.201862335163081E-4 0.0 0.0 16-17 0.0 0.0 2.201862335163081E-4 0.0 0.0 18-19 0.0 1.1009311675815405E-4 2.201862335163081E-4 0.0 0.0 20-21 0.0 2.201862335163081E-4 2.201862335163081E-4 4.403724670326162E-4 0.0 22-23 0.0 2.201862335163081E-4 2.201862335163081E-4 0.003302793502744621 0.0 24-25 0.0 2.201862335163081E-4 2.201862335163081E-4 0.01166987037636433 1.1009311675815405E-4 26-27 0.0 2.201862335163081E-4 2.201862335163081E-4 0.021578250884598194 2.201862335163081E-4 28-29 0.0 2.201862335163081E-4 2.201862335163081E-4 0.05757870006451457 2.201862335163081E-4 30-31 0.0 2.201862335163081E-4 2.201862335163081E-4 0.13376313686115718 2.201862335163081E-4 32-33 0.0 2.201862335163081E-4 2.201862335163081E-4 0.2640032939860534 2.201862335163081E-4 34-35 0.0 2.201862335163081E-4 2.201862335163081E-4 0.47119853972489933 2.201862335163081E-4 36-37 0.0 2.201862335163081E-4 2.201862335163081E-4 0.7563397121285182 2.201862335163081E-4 38-39 0.0 2.201862335163081E-4 2.201862335163081E-4 1.138472920396071 2.201862335163081E-4 40-41 0.0 2.201862335163081E-4 2.201862335163081E-4 1.548019314736404 2.201862335163081E-4 42-43 0.0 2.201862335163081E-4 2.201862335163081E-4 1.9388498792278508 2.201862335163081E-4 44-45 0.0 2.201862335163081E-4 2.201862335163081E-4 2.393424358322269 2.201862335163081E-4 46-47 0.0 2.201862335163081E-4 2.201862335163081E-4 2.9012839059276336 2.201862335163081E-4 48-49 0.0 2.201862335163081E-4 2.201862335163081E-4 3.419712392741781 2.201862335163081E-4 50-51 0.0 2.201862335163081E-4 2.201862335163081E-4 3.9449666527949336 2.201862335163081E-4 52-53 0.0 2.201862335163081E-4 2.201862335163081E-4 4.413743143951153 2.201862335163081E-4 54-55 0.0 2.201862335163081E-4 2.201862335163081E-4 4.942190104390294 2.201862335163081E-4 56-57 0.0 2.201862335163081E-4 2.201862335163081E-4 5.53460116566592 2.201862335163081E-4 58-59 0.0 2.201862335163081E-4 2.201862335163081E-4 6.181288133503317 2.201862335163081E-4 60-61 0.0 2.201862335163081E-4 2.201862335163081E-4 6.844158789504163 2.201862335163081E-4 62-63 0.0 2.201862335163081E-4 2.201862335163081E-4 7.503176186418473 2.201862335163081E-4 64-65 0.0 2.201862335163081E-4 2.201862335163081E-4 8.167367959820416 2.201862335163081E-4 66-67 0.0 2.201862335163081E-4 2.201862335163081E-4 8.91732227117696 2.201862335163081E-4 68-69 0.0 2.201862335163081E-4 2.201862335163081E-4 9.721442395978519 2.201862335163081E-4 70-71 0.0 2.201862335163081E-4 2.201862335163081E-4 10.589966994083596 2.201862335163081E-4 72-73 0.0 3.3027935027446216E-4 2.201862335163081E-4 11.443629021426322 2.201862335163081E-4 74-75 0.0 4.403724670326162E-4 2.201862335163081E-4 12.27747428775258 2.201862335163081E-4 76-77 0.0 4.403724670326162E-4 2.201862335163081E-4 13.171320302712033 2.201862335163081E-4 78-79 0.0 4.403724670326162E-4 4.403724670326162E-4 14.138047960965384 2.201862335163081E-4 80-81 0.0 4.403724670326162E-4 4.403724670326162E-4 15.163675436684347 2.201862335163081E-4 82-83 0.0 4.403724670326162E-4 4.403724670326162E-4 16.184348722149196 2.201862335163081E-4 84-85 0.0 4.403724670326162E-4 4.403724670326162E-4 17.20667340436541 2.201862335163081E-4 86-87 0.0 4.403724670326162E-4 4.403724670326162E-4 18.24936531318189 2.201862335163081E-4 88-89 0.0 4.403724670326162E-4 4.403724670326162E-4 19.287983776678317 2.201862335163081E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3275 0.0 41.696087 1 GTATCAA 5300 0.0 37.03724 1 CTGTTCG 60 0.0040070196 31.57156 9 GTCGGGA 260 0.0 30.984901 2 TATCAAC 6490 0.0 29.864357 2 ATCAACG 6610 0.0 29.32219 3 TCAACGC 6685 0.0 28.99322 4 CAACGCA 6760 0.0 28.741652 5 AACGCAG 6725 0.0 28.731293 6 TAAGGTG 105 7.007811E-5 27.079243 5 GTATGAT 90 9.033315E-4 26.341566 1 GGTCGGG 295 0.0 25.716513 1 GTATAGG 130 1.0143871E-5 25.531057 1 GGACCAT 130 1.0239803E-5 25.500107 6 ACGCAGA 7610 0.0 25.203314 7 CCCTATA 95 0.0012422851 24.941408 2 CGCAGAG 7580 0.0 24.862988 8 GCAGAGT 7685 0.0 23.909758 9 GTACATG 3125 0.0 23.821205 1 CGGGAGT 300 0.0 23.694338 4 >>END_MODULE