FastQCFastQC Report
Thu 26 May 2016
SRR938833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454161
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52791.1623631267325905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49271.08485757253485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33200.7310182952741429No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT25040.5513463287248355No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT13650.3005542087497605RNA PCR Primer, Index 39 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13010.28646228980471683No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.2470489540052977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.21864492988169396No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.2074154319723622No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG8240.18143345641743788RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT7260.1598552055328397RNA PCR Primer, Index 39 (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.12396484946968146No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.11647851753012697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32250.043.8609921
GTATCAA49200.033.0096551
GTATGGC1102.7470596E-630.201213
GCGGTAT700.00827790727.2153381
GACCGCA700.00842167727.1194557
TATCAAC61700.026.3063092
ATCAACG63500.025.7100963
AACGCAG63500.025.560626
TCAACGC63700.025.554874
CAACGCA64650.025.1793525
ATGGGGA4150.025.1590125
GTATAGG1151.2571088E-424.8487831
TACATGG30400.024.6662122
GTACATG30800.024.586581
TACCTGG2900.024.5477832
GTATATG1000.001626656523.813421
CGCAGAA1801.9619074E-723.7295239
CATGGGG8000.023.1362844
AGGGGTC1652.4427463E-623.0104475
ACATGGG30850.022.4603583
Produced by FastQC (version 0.11.5)