##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938833_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 454161 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84879370971968 31.0 31.0 34.0 30.0 34.0 2 32.042586219424386 33.0 31.0 34.0 30.0 34.0 3 32.07566479728555 33.0 31.0 34.0 30.0 34.0 4 35.78653605219294 37.0 35.0 37.0 35.0 37.0 5 35.70838315046866 37.0 35.0 37.0 33.0 37.0 6 35.760884355988296 37.0 35.0 37.0 33.0 37.0 7 35.682326311594345 37.0 35.0 37.0 33.0 37.0 8 35.67563485195779 37.0 35.0 37.0 33.0 37.0 9 37.2536743577718 39.0 37.0 39.0 33.0 39.0 10-11 37.203401436935366 39.0 37.0 39.0 33.0 39.0 12-13 37.11787670011296 39.0 37.0 39.0 33.0 39.0 14-15 38.20428548466293 40.0 37.5 41.0 33.0 41.0 16-17 38.15652598968207 40.0 37.0 41.0 33.0 41.0 18-19 38.26114637760618 40.0 37.5 41.0 33.0 41.0 20-21 38.33629043444946 40.0 38.0 41.0 33.0 41.0 22-23 38.244634611954794 40.0 37.5 41.0 33.0 41.0 24-25 38.12047930139312 40.0 37.0 41.0 33.0 41.0 26-27 37.8623395668056 40.0 37.0 41.0 33.0 41.0 28-29 37.59709222060018 40.0 36.0 41.0 32.0 41.0 30-31 37.18499166595106 39.0 35.5 41.0 32.0 41.0 32-33 36.71015895244197 39.0 35.0 41.0 31.0 41.0 34-35 36.40748435026345 39.0 35.0 41.0 30.0 41.0 36-37 36.11883010650408 39.0 35.0 41.0 30.0 41.0 38-39 35.832788372405375 39.0 35.0 41.0 29.5 41.0 40-41 35.47006678248462 39.0 35.0 41.0 27.5 41.0 42-43 35.041811163882414 38.0 35.0 41.0 25.5 41.0 44-45 34.450262131711 38.0 34.5 40.5 23.0 41.0 46-47 33.69777898146252 38.0 33.5 40.0 12.5 41.0 48-49 33.02473021681739 38.0 33.0 40.0 2.0 41.0 50-51 32.56192187352062 38.0 33.0 40.0 2.0 41.0 52-53 32.24668674765117 38.0 32.5 40.0 2.0 41.0 54-55 31.9248262180152 37.0 32.0 40.0 2.0 41.0 56-57 31.558692842406106 37.0 31.0 40.0 2.0 41.0 58-59 31.15184813315102 36.0 31.0 40.0 2.0 41.0 60-61 30.71556342354363 36.0 30.5 39.5 2.0 41.0 62-63 30.260786373114378 35.0 29.5 39.0 2.0 41.0 64-65 29.817638458608293 35.0 29.0 39.0 2.0 40.0 66-67 29.338944779494497 35.0 28.0 38.5 2.0 40.0 68-69 28.853101653378427 35.0 27.5 38.0 2.0 40.0 70-71 28.39113882521837 34.5 26.0 37.0 2.0 39.5 72-73 28.074466984175217 34.0 26.0 37.0 2.0 39.0 74-75 27.68484744396811 34.0 26.0 36.0 2.0 39.0 76-77 25.831360464681026 31.5 22.5 34.5 2.0 36.5 78-79 26.728948544679092 33.5 24.0 35.0 2.0 37.0 80-81 26.752073603854143 34.0 24.0 35.0 2.0 37.0 82-83 26.45209628303619 34.0 23.5 35.0 2.0 36.5 84-85 26.096040831335145 33.5 21.5 35.0 2.0 36.0 86-87 25.599553902690893 33.0 19.0 35.0 2.0 35.5 88-89 25.114933911102007 33.0 14.5 35.0 2.0 35.0 90-91 24.739158580327242 33.0 8.5 35.0 2.0 35.0 92-93 24.46752032869401 32.5 3.5 35.0 2.0 35.0 94-95 24.13515141106348 32.0 2.0 35.0 2.0 35.0 96-97 23.6822294296516 32.0 2.0 35.0 2.0 35.0 98-99 23.041380699795887 32.0 2.0 34.0 2.0 35.0 100-101 20.53238939495025 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.013395274223075404 1101 2 0.08959830929372714 1101 3 0.19005855820463324 1101 4 0.05351437548972626 1101 5 0.08509188984997706 1101 6 0.11393140735681584 1101 7 -0.1001849808039168 1101 8 -0.019398859368941146 1101 9 0.04060819009133354 1101 10-11 -0.1364699111191996 1101 12-13 0.014784853593369007 1101 14-15 0.03556936228486052 1101 16-17 0.09593837955581819 1101 18-19 0.07436835782353768 1101 20-21 0.2371514548810012 1101 22-23 0.3781755576109589 1101 24-25 0.020526529630210177 1101 26-27 0.05764615189452371 1101 28-29 -0.2540650484118103 1101 30-31 -0.6555029533836958 1101 32-33 -0.5457750439209903 1101 34-35 -0.6033336535195275 1101 36-37 -0.5120547595680662 1101 38-39 -0.727940426592717 1101 40-41 -0.9434742335474766 1101 42-43 -0.5773988627156896 1101 44-45 -0.5719908329151338 1101 46-47 -0.3520523145764045 1101 48-49 -0.48270211033730703 1101 50-51 -0.8291172342445066 1101 52-53 -0.699534017998694 1101 54-55 -0.6976077420227895 1101 56-57 -0.5432807191928326 1101 58-59 -0.35460214326199235 1101 60-61 -0.6662761172507103 1101 62-63 -0.5448976031037169 1101 64-65 -0.8782429638546567 1101 66-67 -0.9062546210992473 1101 68-69 -0.4174760770757615 1101 70-71 -0.3089294483504581 1101 72-73 0.014129108757273912 1101 74-75 0.22233308210339686 1101 76-77 -0.15563175396871998 1101 78-79 -0.15612161259110735 1101 80-81 -0.07642074582275882 1101 82-83 -0.07224790608688281 1101 84-85 -0.19652254496082122 1101 86-87 -0.2831772128546888 1101 88-89 -0.26238362176679786 1101 90-91 0.017753995444067527 1101 92-93 0.11502493707106609 1101 94-95 -0.10347164838549716 1101 96-97 0.10389629256493649 1101 98-99 0.4446821332739077 1101 100-101 0.9992315337752871 1102 1 0.06751292128189945 1102 2 0.00812772105842896 1102 3 -0.03807869669732611 1102 4 -0.08864248725537749 1102 5 -0.023143404267663925 1102 6 0.1060882701019139 1102 7 0.04285423488235551 1102 8 0.054032513180075625 1102 9 0.08217681754231165 1102 10-11 0.09299087319452326 1102 12-13 0.16630446143650346 1102 14-15 0.08929485248093982 1102 16-17 -0.004502796914778173 1102 18-19 -0.08484732845096943 1102 20-21 -0.10054462355037685 1102 22-23 -0.10339306984002405 1102 24-25 -0.20491464684038618 1102 26-27 -0.11745188732116674 1102 28-29 -0.019065048411810892 1102 30-31 -0.36408138475623986 1102 32-33 -0.20812798509745534 1102 34-35 -0.41539247704893967 1102 36-37 -0.3354861321170759 1102 38-39 -0.1395580736515356 1102 40-41 -0.005238939429830225 1102 42-43 -0.29038905879412624 1102 44-45 -0.8190986760523842 1102 46-47 -1.1596993733999312 1102 48-49 -1.21353544367064 1102 50-51 -1.4260289989503896 1102 52-53 -1.3378183317241827 1102 54-55 -1.156872447905144 1102 56-57 -0.9790650329183244 1102 58-59 -1.255141358948265 1102 60-61 -1.4863251368585537 1102 62-63 -1.0512211325154794 1102 64-65 -0.9693704148350513 1102 66-67 -1.0864997191384624 1102 68-69 -1.1387015672718412 1102 70-71 -1.1715765071739845 1102 72-73 -1.2249395186937093 1102 74-75 -0.9102649571122896 1102 76-77 -0.7680337147530345 1102 78-79 -1.1487686714146363 1102 80-81 -0.9822050595482494 1102 82-83 -1.0094047688319812 1102 84-85 -0.9132872508431724 1102 86-87 -1.5512164285409646 1102 88-89 -1.0853738178452268 1102 90-91 -0.9866577692618144 1102 92-93 -1.2135534943014825 1102 94-95 -0.9822461581894153 1102 96-97 -1.1097311584154568 1102 98-99 -0.8734551216280515 1102 100-101 -0.6414057211266737 1103 1 -0.06144592986419539 1103 2 -0.01551768076426896 1103 3 -0.11569261550428322 1103 4 -0.03161404207448726 1103 5 -0.02951733412130153 1103 6 0.026524613653421625 1103 7 0.16317458837586685 1103 8 0.014673220167097156 1103 9 -0.05011260526906369 1103 10-11 -0.06385530189523081 1103 12-13 0.03061266359060255 1103 14-15 -0.11804842285736328 1103 16-17 -0.05890489578249003 1103 18-19 0.020308705793716797 1103 20-21 0.04792541235130443 1103 22-23 -0.06624587293308792 1103 24-25 0.109780188840368 1103 26-27 -0.18151430101904253 1103 28-29 -0.3691230434408084 1103 30-31 -0.3074119564215252 1103 32-33 -0.2317043452189722 1103 34-35 -0.44015359276282595 1103 36-37 -0.427789363268694 1103 38-39 -0.17883451662309113 1103 40-41 -0.5721679645610109 1103 42-43 -0.2724244081451346 1103 44-45 -0.6224430560579037 1103 46-47 -0.6271834938086585 1103 48-49 -0.5758911465151542 1103 50-51 -0.6800389409553595 1103 52-53 -0.82489648692993 1103 54-55 -1.0978252233815304 1103 56-57 -0.8796864082290092 1103 58-59 -0.49684530813357597 1103 60-61 -0.5386697930308806 1103 62-63 -0.9391774981603298 1103 64-65 -1.0196576283119931 1103 66-67 -0.697524297984085 1103 68-69 -0.8423414457584464 1103 70-71 -1.1111594952988177 1103 72-73 -0.8901618329715291 1103 74-75 -1.2044654542125386 1103 76-77 -1.1333140240598532 1103 78-79 -1.1807349790644572 1103 80-81 -1.0733483901199143 1103 82-83 -1.0058643103950864 1103 84-85 -1.1121190652590798 1103 86-87 -1.1182365887177106 1103 88-89 -0.6617726027112845 1103 90-91 -0.682225292045576 1103 92-93 -0.7788061869278273 1103 94-95 -0.7690509082584569 1103 96-97 -0.33179136277889043 1103 98-99 0.014586855726268055 1103 100-101 -0.23742615747022455 1104 1 -0.059762264654544595 1104 2 -0.04412380023974549 1104 3 0.00873671912417251 1104 4 0.0445556195735648 1104 5 -0.01889729202967061 1104 6 -0.12366156086495295 1104 7 -0.1926291998708578 1104 8 0.05249093102997904 1104 9 -0.008749484012788855 1104 10-11 0.05583062978681852 1104 12-13 -0.07633245539919642 1104 14-15 -0.09181400485510238 1104 16-17 -4.865697342495423E-4 1104 18-19 0.0484048689797234 1104 20-21 -0.09828634092697541 1104 22-23 -0.03730787714225414 1104 24-25 -0.17365027902429375 1104 26-27 0.07931619922379696 1104 28-29 0.026344011763981712 1104 30-31 -0.03439916704157042 1104 32-33 -0.1597033738736613 1104 34-35 -0.4498346677183065 1104 36-37 -0.478501683165085 1104 38-39 -0.04718484849940552 1104 40-41 0.01977796388321451 1104 42-43 -0.019057077725832983 1104 44-45 0.1814827979165159 1104 46-47 0.25298487271230385 1104 48-49 -0.006949912906613065 1104 50-51 0.1358506494606999 1104 52-53 -0.10897451833675831 1104 54-55 -0.37172031808769646 1104 56-57 -0.008138731363217744 1104 58-59 -0.25895474637220417 1104 60-61 -0.17480383868410954 1104 62-63 -0.05396623055469618 1104 64-65 0.4370122761723856 1104 66-67 0.31358141676417617 1104 68-69 0.1164573238708222 1104 70-71 -0.16496393110907803 1104 72-73 -0.1820591941500993 1104 74-75 -0.2143893654963307 1104 76-77 -0.03836501968880057 1104 78-79 0.38186013183079837 1104 80-81 0.1321154272671663 1104 82-83 0.02411788160750561 1104 84-85 -0.08115743339895332 1104 86-87 0.2579654511074452 1104 88-89 0.33311164530555004 1104 90-91 0.2969798237064083 1104 92-93 0.2106385273347584 1104 94-95 -0.1242880784057796 1104 96-97 0.1443459206920501 1104 98-99 0.15771458763496327 1104 100-101 0.08492287927900577 1105 1 -0.09498506421367026 1105 2 -0.053397930298004326 1105 3 -0.01713322261728223 1105 4 -0.020552237456826106 1105 5 0.05151681598481872 1105 6 -0.21040380618065058 1105 7 -0.22350622711066137 1105 8 -0.018962383408698713 1105 9 0.03966808802311306 1105 10-11 0.10528740824690885 1105 12-13 -0.27196306475205745 1105 14-15 -0.2731870169791719 1105 16-17 -0.25856403784951 1105 18-19 -0.2518470598503768 1105 20-21 -0.045231703861958295 1105 22-23 -0.04010271260123943 1105 24-25 0.03430909745185318 1105 26-27 0.0017772289828883459 1105 28-29 -0.14034627323050586 1105 30-31 -0.30509669498992054 1105 32-33 0.0668357538227653 1105 34-35 0.04093843887908122 1105 36-37 -0.04935666663225646 1105 38-39 -0.1555693548763557 1105 40-41 -0.04211780056333225 1105 42-43 -0.3223793891728448 1105 44-45 0.20733699410608608 1105 46-47 0.4900105379618722 1105 48-49 0.22887121762401108 1105 50-51 0.03696858364429545 1105 52-53 -0.11165126215662724 1105 54-55 0.2255557014367895 1105 56-57 -0.09582570979181426 1105 58-59 -0.3443113831223208 1105 60-61 -0.20980620051689058 1105 62-63 -0.3685781027008126 1105 64-65 -0.28784207747270685 1105 66-67 -0.1843401703874541 1105 68-69 -2.0396173265879725E-5 1105 70-71 -0.07091574972032078 1105 72-73 -0.3687778211379751 1105 74-75 -0.36296439305104755 1105 76-77 -0.5463952511484145 1105 78-79 -0.4102513466765245 1105 80-81 -0.1323232438082549 1105 82-83 -0.03655760256821594 1105 84-85 0.432305013459807 1105 86-87 0.36851497086811236 1105 88-89 0.20093561003551486 1105 90-91 0.43331268467318296 1105 92-93 0.21254373911243007 1105 94-95 0.3138161571476772 1105 96-97 0.2606583124413788 1105 98-99 0.19384275642727644 1105 100-101 0.5623358851048579 1106 1 0.06592605397683826 1106 2 -0.13225531587727346 1106 3 -0.04811973636901001 1106 4 0.0031495784080988187 1106 5 0.08249271064341457 1106 6 -0.046168911840624105 1106 7 0.23885970683858204 1106 8 -0.08046817081444857 1106 9 0.19665014175571827 1106 10-11 -0.016502970854716636 1106 12-13 -0.009331653474575319 1106 14-15 0.1924549072005064 1106 16-17 -0.08328528665485635 1106 18-19 0.048832562109900834 1106 20-21 -0.0383558410606355 1106 22-23 -0.003105792138242691 1106 24-25 0.01555046943868632 1106 26-27 0.20242499366379008 1106 28-29 0.00855465063059313 1106 30-31 -0.1729049141680079 1106 32-33 -0.18421553639704769 1106 34-35 0.006139670693883659 1106 36-37 -0.2229416724727642 1106 38-39 -0.34690417488273084 1106 40-41 -0.3646780639168412 1106 42-43 -0.41884323116074285 1106 44-45 -0.8089208374750996 1106 46-47 -0.5788649000757218 1106 48-49 -0.6945067159004594 1106 50-51 -0.34472940934710294 1106 52-53 -0.3493915191386847 1106 54-55 -0.20391759154399125 1106 56-57 -0.3426081245735908 1106 58-59 -0.31288417700572424 1106 60-61 -0.12468354999124642 1106 62-63 -0.41016778094229167 1106 64-65 -0.3382349839185004 1106 66-67 -0.1666912376063152 1106 68-69 -0.246266546752004 1106 70-71 -0.4002085181178998 1106 72-73 -0.2735988894187429 1106 74-75 -0.2920023032135184 1106 76-77 -0.5611664096914772 1106 78-79 -0.33502038140095536 1106 80-81 -0.0836824877288258 1106 82-83 0.09883052528566694 1106 84-85 -0.24819833155452997 1106 86-87 -0.18436280336996447 1106 88-89 -0.1254422172980334 1106 90-91 -0.1681899170046357 1106 92-93 0.10042735385173174 1106 94-95 0.08311635891044844 1106 96-97 -0.11784333351805643 1106 98-99 -0.2745495128729232 1106 100-101 0.06784188489247711 1107 1 -0.10556480981053795 1107 2 -0.04695631255501098 1107 3 -0.15964382274774636 1107 4 -0.06907841162512085 1107 5 -0.1000446647718718 1107 6 0.04009562304309355 1107 7 -0.10469303402520325 1107 8 0.036973689650665165 1107 9 -0.058831585819028476 1107 10-11 0.05553814210209396 1107 12-13 -0.0971067780592989 1107 14-15 -0.12956544163670713 1107 16-17 0.10926558543816611 1107 18-19 -0.06962674021567494 1107 20-21 0.03831567056727181 1107 22-23 -0.14587468748707977 1107 24-25 -0.04223607541181451 1107 26-27 -0.079915122615283 1107 28-29 -0.11586914505046053 1107 30-31 0.029327228689133733 1107 32-33 0.24611046028068984 1107 34-35 0.2640508002619839 1107 36-37 0.1982481115803978 1107 38-39 0.04162364903753968 1107 40-41 0.10018385468781332 1107 42-43 -0.0682514537521044 1107 44-45 -0.575054558405327 1107 46-47 -0.4675328818032938 1107 48-49 -0.6842077125782069 1107 50-51 -0.6007821502108968 1107 52-53 -0.267264235085527 1107 54-55 -0.4101208722748879 1107 56-57 -0.3993512724141226 1107 58-59 -0.15852371188944403 1107 60-61 -0.17242545198460135 1107 62-63 -0.1610346829356466 1107 64-65 -0.07922335601152142 1107 66-67 -0.13855066451661102 1107 68-69 -0.36920051685167365 1107 70-71 -0.4015633769218887 1107 72-73 -0.37303825818950287 1107 74-75 -0.4193642218181708 1107 76-77 -0.4879365508874862 1107 78-79 -0.28427287309531124 1107 80-81 -0.2847103116490928 1107 82-83 -0.4174641175714804 1107 84-85 -0.7038098348767861 1107 86-87 -0.6408671638350825 1107 88-89 -0.15134545650068887 1107 90-91 -0.13547604657273737 1107 92-93 -0.17972733883929592 1107 94-95 -0.2581679018868961 1107 96-97 -0.4384050029532709 1107 98-99 -0.5497156258297302 1107 100-101 -0.5304866034796163 1108 1 0.0513009537362592 1108 2 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-0.48311244937124087 2316 54-55 -0.7767548008463194 2316 56-57 -0.49777091527126416 2316 58-59 -0.586023711889446 2316 60-61 -0.41814866627031577 2316 62-63 -0.31963289722136423 2316 64-65 -0.15372335601152187 2316 66-67 -0.12797030737375437 2316 68-69 -0.01228980256595591 2316 70-71 0.01007055164954096 2316 72-73 -0.30664540104664795 2316 74-75 -0.4826767218181729 2316 76-77 -0.2504454794589144 2316 78-79 -0.2894157302381686 2316 80-81 -0.5782638830776605 2316 82-83 -0.9391694747143333 2316 84-85 -0.9406401920196465 2316 86-87 -1.1078046638350827 2316 88-89 -1.1002561707864054 2316 90-91 -1.1014224751441688 2316 92-93 -0.9272005531250116 2316 94-95 -0.9750696876011808 2316 96-97 -1.4423782172389856 2316 98-99 -1.976572768686875 2316 100-101 -2.8741116034796192 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 3.0 5 28.0 6 114.0 7 278.0 8 538.0 9 927.0 10 1651.0 11 2608.0 12 3783.0 13 5255.0 14 5816.0 15 7189.0 16 8855.0 17 11428.0 18 10235.0 19 5970.0 20 4790.0 21 4805.0 22 4978.0 23 5108.0 24 5482.0 25 6100.0 26 6610.0 27 6988.0 28 8274.0 29 9705.0 30 11635.0 31 13855.0 32 16388.0 33 20271.0 34 26995.0 35 37043.0 36 52513.0 37 76352.0 38 65300.0 39 6286.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.826445586876666 20.465646729112425 15.524946623464459 20.182961060546454 2 16.46376505248139 22.786632934135692 38.49648032305724 22.253121690325678 3 19.218955392470953 27.427057805491884 32.32664187369677 21.02734492834039 4 12.872747770063919 19.337855958569758 35.650573254858955 32.13882301650736 5 12.188849328762268 39.062138756960636 33.16180825742413 15.587203656852965 6 28.08563482993916 37.25396940732472 17.556108956955793 17.104286805780326 7 24.13483324195605 36.97917698789636 20.863085998137223 18.022903772010366 8 26.15041802356433 33.622878230407274 20.320987491220073 19.90571625480832 9 26.303227269624646 16.30743282668481 21.127749850823825 36.261590052866715 10-11 23.876664883158174 28.078258591116366 26.397577070686385 21.64749945503907 12-13 26.014453024368013 26.654754591433434 27.43773683781743 19.89305554638113 14-15 23.021266710777358 27.072697867824257 28.06595906072853 21.84007636066985 16-17 19.67607832602669 30.37687739594366 28.800426288057107 21.14661798997254 18-19 19.161397787786477 27.299489938028515 33.65330098060047 19.88581129358454 20-21 20.634202408397023 26.27537371108484 33.78636650879314 19.304057371725005 22-23 20.175554483982552 27.032814354380935 33.4531146443662 19.338516517270307 24-25 19.67606201325081 27.433663392497376 33.93389128524906 18.956383309002756 26-27 19.392241958248285 27.48496678490667 33.96449717170783 19.15829408513721 28-29 18.750509180665006 27.139274398286073 34.358410343468506 19.751806077580415 30-31 19.076384806269143 27.33380893559773 34.16618776160877 19.423618496524362 32-33 18.73355484068425 27.77913559288446 33.86607392532604 19.621235641105248 34-35 19.07457809252017 27.88189673396047 33.8388075608639 19.20471761265546 36-37 19.494684218929798 27.4979215874117 33.14118340132908 19.86621079232942 38-39 19.426199995375423 27.161907572305825 33.45243456497587 19.959457867342884 40-41 19.318153041214924 27.15849022118627 33.59115827441896 19.932198463179844 42-43 19.561983844135653 27.24433185030558 33.18194779786342 20.01173650769534 44-45 19.278021025884364 27.708412005165645 32.50765983822686 20.505907130723138 46-47 19.816933537670206 27.412958657013508 31.602635241064664 21.167472564251614 48-49 19.837128243067987 27.499388983201996 31.15789334619221 21.50558942753781 50-51 19.82684497406748 27.910678957502817 30.68206213896219 21.58041392946751 52-53 19.807623287776803 28.10005702823448 30.71851171721042 21.3738079667783 54-55 19.267616550078056 28.172498299061346 30.361920112030756 22.197965038829842 56-57 19.86674329147593 28.51686956828085 29.585213173301977 22.03117396694124 58-59 19.32334494083039 28.38754140881913 30.200556851597614 22.088556798752865 60-61 19.59106787608291 28.756370298034717 29.669817915991885 21.98274390989049 62-63 19.268717481245638 28.767221315788895 29.74341698208345 22.220644220882022 64-65 19.218074647536888 28.652284101893382 29.631892654807434 22.497748595762296 66-67 19.096097570642492 28.67822077581784 29.25754096086561 22.968140692674055 68-69 19.202983115933733 28.44551211477994 29.05774667735316 23.293758091933167 70-71 19.024645879252148 28.233216559177194 28.870006363494237 23.872131198076417 72-73 18.983576308842018 27.974000409546395 28.564649980953895 24.477773300657695 74-75 18.729151116013927 27.79234676689544 28.54329191630281 24.93521020078783 76-77 18.534256191106934 27.640222111780982 28.940428950346625 24.885092746765462 78-79 18.5016068529691 27.4020744813246 28.76024520201853 25.336073463687764 80-81 18.232961438784926 27.590876363228016 28.554741600445656 25.6214205975414 82-83 18.23450274241954 27.21006427236156 28.386629411155955 26.16880357406294 84-85 18.124629811894902 26.75945314547044 28.437932803565257 26.677984239069403 86-87 18.061086781069317 26.61802128400877 28.49437468852401 26.826517246397902 88-89 18.063241281349477 26.313568776108458 28.574221468192697 27.048968474349373 90-91 17.88004380832093 26.157696706868457 28.618492234180202 27.343767250630407 92-93 17.75746926750646 26.00388408515923 28.71503717844553 27.523609468888786 94-95 17.564696220062928 25.598961601722735 28.883809926435777 27.952532251778557 96-97 17.568329292915948 25.29576515817078 28.92751689378877 28.208388655124505 98-99 17.439357758672887 25.245976358526022 28.911758974545286 28.402906908255797 100-101 17.367251156814888 24.848923223602437 29.564588418309544 28.219237201273128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 996.0 1 708.0 2 486.0 3 539.5 4 467.0 5 476.5 6 735.5 7 1249.0 8 1754.0 9 1907.5 10 1688.0 11 1461.0 12 1456.5 13 1486.0 14 1541.0 15 1583.0 16 1642.0 17 1737.5 18 1827.5 19 2005.5 20 2168.0 21 2305.0 22 2568.5 23 2843.5 24 3106.5 25 3571.0 26 4211.0 27 4876.5 28 5534.0 29 6226.5 30 6900.5 31 7674.5 32 8643.0 33 9423.5 34 10167.0 35 10946.0 36 11586.5 37 12199.0 38 12351.5 39 12088.0 40 11950.0 41 12294.5 42 12844.5 43 13395.0 44 14008.0 45 14350.5 46 14547.5 47 14859.0 48 15506.0 49 15835.0 50 15445.0 51 14606.5 52 13370.5 53 12640.5 54 12127.5 55 11229.0 56 10363.0 57 9392.0 58 8538.0 59 7900.0 60 7048.5 61 6005.5 62 4995.0 63 3853.5 64 2831.5 65 2238.0 66 1765.0 67 1331.5 68 1038.5 69 776.0 70 548.0 71 443.0 72 360.5 73 299.0 74 240.5 75 176.0 76 122.5 77 83.5 78 55.0 79 34.0 80 23.0 81 16.0 82 9.0 83 7.5 84 5.0 85 2.5 86 3.0 87 2.5 88 2.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3776193904804684 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1009311675815404E-4 16-17 0.0017614898681304646 18-19 1.1009311675815404E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007266145706038168 36-37 0.019486481666193267 38-39 0.01442219829531818 40-41 0.009137728690926786 42-43 0.004954190254116932 44-45 0.0014312105178560025 46-47 0.001981676101646773 48-49 0.0 50-51 3.3027935027446216E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.1009311675815404E-4 60-61 5.504655837907703E-4 62-63 0.0 64-65 0.0 66-67 0.0017614898681304646 68-69 0.0011009311675815405 70-71 0.0017614898681304646 72-73 0.0 74-75 0.0 76-77 0.0936892423611891 78-79 0.03699128723073976 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14862570762350796 88-89 0.32631599807116857 90-91 0.28161819266735805 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.3027935027446216E-4 100-101 9.908380508233865E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 454161.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.66460305924008 #Duplication Level Percentage of deduplicated Percentage of total 1 86.44661604739309 58.49375960661387 2 8.500812054303521 11.50408146671302 3 2.07026958509946 4.202519091041179 4 0.9046396685628908 2.4484833633980205 5 0.5005724177200702 1.6935516973716334 6 0.3164858435141748 1.2848933385153247 7 0.21730877521946163 1.0292878411560147 8 0.15984487634879246 0.8652672087355482 9 0.12914098497723736 0.7864446138447646 >10 0.6934227691357225 8.420409945357338 >50 0.03972305508267013 1.8112107945480855 >100 0.01693113811437243 2.0855031368118873 >500 0.001953592859350665 1.0083824062104383 >1k 0.001953592859350665 3.20316185359265 >5k 3.2559880989177747E-4 1.1630436360902126 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5279 1.1623631267325905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4927 1.08485757253485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3320 0.7310182952741429 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2504 0.5513463287248355 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1365 0.3005542087497605 RNA PCR Primer, Index 39 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1301 0.28646228980471683 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.2470489540052977 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 993 0.21864492988169396 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 0.2074154319723622 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 824 0.18143345641743788 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 726 0.1598552055328397 RNA PCR Primer, Index 39 (96% over 25bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 0.12396484946968146 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.11647851753012697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.201862335163081E-4 0.0 5 0.0 0.0 0.0 2.201862335163081E-4 0.0 6 0.0 0.0 0.0 2.201862335163081E-4 0.0 7 0.0 0.0 0.0 2.201862335163081E-4 0.0 8 0.0 0.0 0.0 2.201862335163081E-4 0.0 9 0.0 0.0 0.0 2.201862335163081E-4 0.0 10-11 0.0 0.0 0.0 2.201862335163081E-4 0.0 12-13 0.0 0.0 0.0 2.201862335163081E-4 0.0 14-15 0.0 0.0 0.0 2.201862335163081E-4 0.0 16-17 0.0 0.0 0.0 2.201862335163081E-4 0.0 18-19 0.0 0.0 0.0 2.201862335163081E-4 0.0 20-21 0.0 0.0 0.0 6.605587005489243E-4 0.0 22-23 0.0 0.0 0.0 0.0037431659697772374 0.0 24-25 0.0 0.0 0.0 0.012550615310429562 0.0 26-27 0.0 0.0 0.0 0.022238809585147117 0.0 28-29 1.1009311675815405E-4 0.0 0.0 0.058019072531547186 0.0 30-31 2.201862335163081E-4 0.0 0.0 0.13552462672928764 0.0 32-33 2.201862335163081E-4 0.0 0.0 0.2670859012552817 0.0 34-35 2.201862335163081E-4 0.0 0.0 0.47494170569467653 0.0 36-37 2.201862335163081E-4 0.0 0.0 0.7638260440680729 0.0 38-39 2.201862335163081E-4 0.0 0.0 1.1530052118081473 0.0 40-41 2.201862335163081E-4 0.0 0.0 1.5688269138036952 0.0 42-43 2.201862335163081E-4 0.0 0.0 1.9657125997168405 0.0 44-45 2.201862335163081E-4 0.0 0.0 2.423699965430761 0.0 46-47 2.201862335163081E-4 0.0 0.0 2.93607333082321 0.0 48-49 2.201862335163081E-4 0.0 0.0 3.458244983607135 0.0 50-51 2.201862335163081E-4 0.0 0.0 3.986251571579242 0.0 52-53 2.201862335163081E-4 0.0 0.0 4.458881321821997 0.0 54-55 2.201862335163081E-4 0.0 0.0 4.993273310566076 1.1009311675815405E-4 56-57 2.201862335163081E-4 0.0 0.0 5.5904183758623045 2.201862335163081E-4 58-59 2.201862335163081E-4 0.0 0.0 6.2455825136900796 2.201862335163081E-4 60-61 2.201862335163081E-4 0.0 0.0 6.917480805265093 2.201862335163081E-4 62-63 2.201862335163081E-4 0.0 0.0 7.579580809448632 2.201862335163081E-4 64-65 2.201862335163081E-4 0.0 0.0 8.250598356089581 2.201862335163081E-4 66-67 2.201862335163081E-4 0.0 0.0 9.008919744319746 2.201862335163081E-4 68-69 2.201862335163081E-4 0.0 0.0 9.818984897426244 2.201862335163081E-4 70-71 2.201862335163081E-4 0.0 0.0 10.7007206695423 2.201862335163081E-4 72-73 2.201862335163081E-4 0.0 0.0 11.566382846611663 2.201862335163081E-4 74-75 2.201862335163081E-4 0.0 0.0 12.412118169547803 2.201862335163081E-4 76-77 2.201862335163081E-4 0.0 0.0 13.316312937482522 2.201862335163081E-4 78-79 2.201862335163081E-4 0.0 0.0 14.287774599756474 2.201862335163081E-4 80-81 2.201862335163081E-4 0.0 0.0 15.318796638196586 4.403724670326162E-4 82-83 2.201862335163081E-4 0.0 0.0 16.353121470139445 6.605587005489243E-4 84-85 2.201862335163081E-4 0.0 0.0 17.384693974163348 6.605587005489243E-4 86-87 2.201862335163081E-4 0.0 0.0 18.436963984137783 6.605587005489243E-4 88-89 2.201862335163081E-4 0.0 0.0 19.485931200609475 6.605587005489243E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3225 0.0 43.860992 1 GTATCAA 4920 0.0 33.009655 1 GTATGGC 110 2.7470596E-6 30.20121 3 GCGGTAT 70 0.008277907 27.215338 1 GACCGCA 70 0.008421677 27.119455 7 TATCAAC 6170 0.0 26.306309 2 ATCAACG 6350 0.0 25.710096 3 AACGCAG 6350 0.0 25.56062 6 TCAACGC 6370 0.0 25.55487 4 CAACGCA 6465 0.0 25.179352 5 ATGGGGA 415 0.0 25.159012 5 GTATAGG 115 1.2571088E-4 24.848783 1 TACATGG 3040 0.0 24.666212 2 GTACATG 3080 0.0 24.58658 1 TACCTGG 290 0.0 24.547783 2 GTATATG 100 0.0016266565 23.81342 1 CGCAGAA 180 1.9619074E-7 23.729523 9 CATGGGG 800 0.0 23.136284 4 AGGGGTC 165 2.4427463E-6 23.010447 5 ACATGGG 3085 0.0 22.460358 3 >>END_MODULE