FastQCFastQC Report
Thu 26 May 2016
SRR938832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451514
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59751.3233255225751581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50191.111593438963133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.6852500697652786No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12030.26643692111429546No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.25580602151871257No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.23365831402791495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.21151060653711737No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.14750373188871221No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.11140296867871208No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.10608751888092063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.044.0501561
GTATCAA49950.032.153241
TACTAGC804.5183362E-429.654892
TATCAAC60950.026.3123282
ATCAACG63000.025.230193
AACGCAG63500.025.031536
TCAACGC63900.024.9490914
CAACGCA63850.024.8943165
GTCTACT1151.2770624E-424.7911641
GTACATG32550.024.2326341
TACATGG32150.023.908392
GAGTAGG1601.8498031E-623.75821
CTATTAG1401.7716195E-523.75821
ACATGGG31950.022.873
GAGTACT43300.022.75538312-13
CATGGGG9600.022.2411674
ATTAGCT1503.053734E-522.1423173
ACGCAGA72450.021.8762367
CGCAGAG72350.021.8408858
CATGGGA18850.020.8921454
Produced by FastQC (version 0.11.5)