##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938832_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 451514 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45309115553449 33.0 31.0 34.0 30.0 34.0 2 31.466769579680808 33.0 31.0 34.0 28.0 34.0 3 31.427729815686778 33.0 31.0 34.0 28.0 34.0 4 35.0167193043848 37.0 35.0 37.0 32.0 37.0 5 35.04358890311264 37.0 35.0 37.0 33.0 37.0 6 35.146247956873985 37.0 35.0 37.0 33.0 37.0 7 35.07501871481283 37.0 35.0 37.0 32.0 37.0 8 35.046005217999884 37.0 35.0 37.0 32.0 37.0 9 36.5829232316163 39.0 37.0 39.0 32.0 39.0 10-11 36.47499523824289 39.0 37.0 39.0 32.0 39.0 12-13 36.36574391934691 39.0 37.0 39.0 32.0 39.0 14-15 37.426096422259334 40.0 37.0 41.0 32.0 41.0 16-17 37.33002852624725 40.0 37.0 41.0 32.0 41.0 18-19 37.38065929295658 40.0 37.0 41.0 32.0 41.0 20-21 37.337713780746554 40.0 37.0 41.0 32.0 41.0 22-23 37.24173115340831 40.0 37.0 41.0 32.0 41.0 24-25 37.11653680727508 40.0 37.0 41.0 31.0 41.0 26-27 36.91600371195578 40.0 36.0 41.0 31.0 41.0 28-29 36.65806154404957 39.5 36.0 41.0 30.5 41.0 30-31 36.240425546051725 39.0 35.0 41.0 30.0 41.0 32-33 35.83371944170059 38.5 35.0 41.0 29.5 41.0 34-35 35.37416004819341 38.0 35.0 40.5 27.5 41.0 36-37 35.00911156686171 38.0 35.0 40.0 25.5 41.0 38-39 34.69390982339418 38.0 35.0 40.0 24.5 41.0 40-41 34.34373795718405 38.0 34.5 40.0 23.0 41.0 42-43 34.03080635373432 38.0 34.0 40.0 18.0 41.0 44-45 33.51037398618869 38.0 33.5 40.0 9.0 41.0 46-47 32.76160650611055 38.0 33.0 40.0 2.0 41.0 48-49 32.08336840053686 38.0 32.5 40.0 2.0 41.0 50-51 30.8089660564235 36.5 30.0 39.0 2.0 40.5 52-53 30.76684776108825 37.0 30.5 39.5 2.0 40.5 54-55 31.12103943620796 37.0 31.0 40.0 2.0 41.0 56-57 30.950421249396477 37.0 31.0 40.0 2.0 41.0 58-59 30.690778137554982 36.5 30.0 40.0 2.0 41.0 60-61 30.36466200383598 36.0 29.5 40.0 2.0 41.0 62-63 29.95073796161359 35.5 28.5 39.5 2.0 41.0 64-65 29.533750891445226 35.0 28.0 39.0 2.0 41.0 66-67 29.136621455813106 35.0 27.5 39.0 2.0 40.5 68-69 28.68659443560997 35.0 26.0 38.0 2.0 40.0 70-71 28.23611338740327 35.0 26.0 37.5 2.0 40.0 72-73 27.77679983344924 35.0 25.0 37.0 2.0 39.5 74-75 27.276841914093474 34.0 23.5 36.5 2.0 39.0 76-77 26.784077570130716 34.0 21.5 36.0 2.0 39.0 78-79 26.31452956054519 34.0 19.5 35.0 2.0 37.5 80-81 25.801092546410523 34.0 15.5 35.0 2.0 37.0 82-83 25.349524045766024 33.0 7.5 35.0 2.0 36.5 84-85 24.961508391766365 33.0 2.0 35.0 2.0 36.0 86-87 24.47706383412253 33.0 2.0 35.0 2.0 36.0 88-89 23.96992452061287 32.5 2.0 35.0 2.0 35.0 90-91 23.460028924905984 32.0 2.0 35.0 2.0 35.0 92-93 22.95682525901744 32.0 2.0 35.0 2.0 35.0 94-95 22.417212976784775 31.0 2.0 35.0 2.0 35.0 96-97 21.81779413262933 31.0 2.0 34.5 2.0 35.0 98-99 21.089970632139867 30.5 2.0 34.0 2.0 35.0 100-101 19.13585293036318 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.10056362945103459 1101 2 -0.13666275959904794 1101 3 0.16180195877876358 1101 4 -0.23927683755873375 1101 5 -0.35645830935265366 1101 6 -0.09386936207619812 1101 7 -0.1341184909136217 1101 8 -0.16203805715350938 1101 9 -0.11542984175380155 1101 10-11 -0.391555581592371 1101 12-13 -0.18861476673347255 1101 14-15 -0.31003498915677596 1101 16-17 -0.5037843707876561 1101 18-19 -0.5341766212030095 1101 20-21 -0.13309726040848346 1101 22-23 -0.4746134330797034 1101 24-25 -0.6703702275519987 1101 26-27 -0.6602970909361048 1101 28-29 -0.5520297850002507 1101 30-31 -0.597704463213482 1101 32-33 -0.8161965921739167 1101 34-35 -1.0068919069584155 1101 36-37 -1.1002120980461783 1101 38-39 -0.8075333438969778 1101 40-41 -0.9600597126780457 1101 42-43 -0.9673386317153572 1101 44-45 -1.1647352006344107 1101 46-47 -1.1015233905362933 1101 48-49 -0.91851796659234 1101 50-51 -0.5916221197252582 1101 52-53 -0.7101588782637904 1101 54-55 -0.8224216591547595 1101 56-57 -0.9920397753255052 1101 58-59 -0.893610797640914 1101 60-61 -1.1003987388542775 1101 62-63 -1.1645855910044318 1101 64-65 -1.056066442479544 1101 66-67 -1.2498622575502054 1101 68-69 -1.154445282946977 1101 70-71 -1.1217386189091911 1101 72-73 -1.0368582625961764 1101 74-75 -1.4189213572988066 1101 76-77 -1.3584094463022431 1101 78-79 -1.1709422118466897 1101 80-81 -1.1660170056167978 1101 82-83 -1.2978443449228756 1101 84-85 -1.2391571223331148 1101 86-87 -1.2217341623041058 1101 88-89 -1.035926974006557 1101 90-91 -1.1438556161331412 1101 92-93 -1.0377493736897847 1101 94-95 -0.9477613849938287 1101 96-97 -1.1022383491981351 1101 98-99 -1.076816042624266 1101 100-101 -0.6380081402750655 1102 1 0.19286482659957827 1102 2 0.39298053021115464 1102 3 0.27975592297313767 1102 4 0.6742296909107708 1102 5 0.6671195611548129 1102 6 0.5277432371969226 1102 7 0.4293274641274323 1102 8 0.4990038058159314 1102 9 0.6576701717069611 1102 10-11 0.6173473663144833 1102 12-13 0.848628469233681 1102 14-15 0.8369242247347302 1102 16-17 0.9820764449345205 1102 18-19 0.9733721740588024 1102 20-21 0.8387347492832617 1102 22-23 0.799046548075232 1102 24-25 0.8477102678040396 1102 26-27 0.7613155083370131 1102 28-29 0.7558689900704252 1102 30-31 0.9196222294773264 1102 32-33 1.0221477341149523 1102 34-35 1.061615294549192 1102 36-37 0.7995994512875058 1102 38-39 0.8274323311602316 1102 40-41 0.4303912228449107 1102 42-43 0.5747127883950114 1102 44-45 0.5617178886104384 1102 46-47 0.5991092652720269 1102 48-49 1.2578610884621781 1102 50-51 1.1924834126478778 1102 52-53 0.8788679086523494 1102 54-55 0.7643652569846928 1102 56-57 0.7615771313914159 1102 58-59 0.3693963365628825 1102 60-61 0.4833810430813834 1102 62-63 0.16575227411873072 1102 64-65 0.03153619582978351 1102 66-67 0.19590164068643645 1102 68-69 0.19940584102663905 1102 70-71 0.06986186567823793 1102 72-73 0.19990307809570496 1102 74-75 -0.02520753309635637 1102 76-77 0.2724641571437125 1102 78-79 0.22446228404777813 1102 80-81 0.11853273189834468 1102 82-83 0.06219334521038533 1102 84-85 0.3572784679212937 1102 86-87 0.32856062838104805 1102 88-89 0.024991049953595024 1102 90-91 0.22049759425856408 1102 92-93 0.19832277599388703 1102 94-95 0.0645013690780516 1102 96-97 0.08016574287347922 1102 98-99 -0.1386063239541926 1102 100-101 0.17045867894690048 1103 1 0.22096739391721698 1103 2 0.4845137109891873 1103 3 0.3145980909302537 1103 4 0.275302802516471 1103 5 0.37317370783777903 1103 6 0.3982122925034446 1103 7 0.39612056361229264 1103 8 0.28304923803853654 1103 9 0.3465148265244693 1103 10-11 0.4332308809378489 1103 12-13 0.38683782526223354 1103 14-15 0.2129625934379007 1103 16-17 0.5406743990216398 1103 18-19 0.49985775967262924 1103 20-21 0.6270773299756129 1103 22-23 0.5054402870384749 1103 24-25 0.6787004144034157 1103 26-27 0.6557571663832604 1103 28-29 0.8917816573849322 1103 30-31 0.552682321637441 1103 32-33 0.38188828561281696 1103 34-35 0.14830014246409462 1103 36-37 0.4618749285452708 1103 38-39 0.6788136880664908 1103 40-41 0.7377431344882268 1103 42-43 0.7775042369389453 1103 44-45 1.1230273564432167 1103 46-47 1.0056482452413036 1103 48-49 0.4874960006383873 1103 50-51 0.6535269536027037 1103 52-53 0.5301876164984982 1103 54-55 0.5304201350971844 1103 56-57 0.7061713447389586 1103 58-59 0.8155108784267746 1103 60-61 0.49167754371354633 1103 62-63 0.45654253147743873 1103 64-65 0.5987415080979481 1103 66-67 0.26019952058570794 1103 68-69 0.2476148835853884 1103 70-71 0.19817811490762338 1103 72-73 0.5195612915268413 1103 74-75 0.36916620649383347 1103 76-77 0.5691006262199139 1103 78-79 0.5520016506298084 1103 80-81 0.7556966661532769 1103 82-83 0.3728889346903408 1103 84-85 0.12259146751378225 1103 86-87 0.1691951957404818 1103 88-89 0.23844047160182313 1103 90-91 -0.24804309934560465 1103 92-93 -0.24843699120254215 1103 94-95 -0.2483388762643095 1103 96-97 0.015196515158567792 1103 98-99 -0.014226732927788532 1103 100-101 -0.03823645077735094 1104 1 0.016461997955683927 1104 2 0.24975073130521608 1104 3 0.019180792930935553 1104 4 -0.021887380647306998 1104 5 0.0949080430573801 1104 6 0.3168914930559268 1104 7 0.21879081727917793 1104 8 0.14115142631482058 1104 9 0.4167757086379069 1104 10-11 0.2971258684142697 1104 12-13 0.35746410725404587 1104 14-15 0.3961315225946507 1104 16-17 0.27777717482381803 1104 18-19 0.43184648329837927 1104 20-21 0.10005371555752873 1104 22-23 -0.11200820137077017 1104 24-25 0.16902427517008078 1104 26-27 0.14301455426373622 1104 28-29 0.09712968917317255 1104 30-31 -0.11156981973983449 1104 32-33 0.01627452719934297 1104 34-35 0.003619313331057583 1104 36-37 0.30515239538097916 1104 38-39 0.46497229944387897 1104 40-41 0.3676842788237522 1104 42-43 0.34883451926060616 1104 44-45 0.34754470907213886 1104 46-47 0.45776488459316766 1104 48-49 0.7126704546000724 1104 50-51 0.5866649603437928 1104 52-53 0.5914371913152827 1104 54-55 0.1880497257874758 1104 56-57 0.24102090718677616 1104 58-59 0.3522157858141419 1104 60-61 0.2572031732423916 1104 62-63 0.36935678052657295 1104 64-65 0.2767180482919329 1104 66-67 0.39336174988572736 1104 68-69 0.1648536149417481 1104 70-71 0.11436548413157155 1104 72-73 -0.04713578218188985 1104 74-75 0.1292262990549311 1104 76-77 0.031066054959211442 1104 78-79 -0.42616197017891366 1104 80-81 -0.13662117504981097 1104 82-83 -0.04501205173471945 1104 84-85 0.02938357611330744 1104 86-87 -0.2725959336492103 1104 88-89 -0.11847378053955637 1104 90-91 -0.06804629466189382 1104 92-93 -0.016315301189450793 1104 94-95 0.063047272554968 1104 96-97 0.029964545503762707 1104 98-99 0.27532045983224407 1104 100-101 0.6378383608536033 1105 1 -0.08341248199653606 1105 2 0.024872455383807335 1105 3 0.036141183257793585 1105 4 0.15277275357816933 1105 5 0.09820800560382281 1105 6 0.1810818381348298 1105 7 0.08649876047123684 1105 8 0.14184482335294746 1105 9 0.1294765154078945 1105 10-11 0.28374381170755214 1105 12-13 0.31414703753981144 1105 14-15 0.3276041561874621 1105 16-17 0.25784580594671525 1105 18-19 0.13908109443929817 1105 20-21 0.3895115499317967 1105 22-23 0.26901094043652307 1105 24-25 0.19247071151421125 1105 26-27 0.02788280882649019 1105 28-29 -0.021557614069088515 1105 30-31 0.05308160906296422 1105 32-33 -0.11328443179406378 1105 34-35 -0.18006257432849537 1105 36-37 -0.10692536631313487 1105 38-39 -0.007443657798326342 1105 40-41 -0.29252080473818864 1105 42-43 -0.9411977717839477 1105 44-45 -0.5275603127420396 1105 46-47 -0.8536336516811076 1105 48-49 -1.1026303379982991 1105 50-51 -1.0217909406168424 1105 52-53 -0.8442211309675614 1105 54-55 -0.6038223449896343 1105 56-57 -1.0095206510838786 1105 58-59 -0.8792953135997621 1105 60-61 -0.9488951777885966 1105 62-63 -1.0835858547870743 1105 64-65 -1.1928509320601286 1105 66-67 -1.381537277333905 1105 68-69 -0.8487106482859375 1105 70-71 -0.9372622274557507 1105 72-73 -0.8906963000533139 1105 74-75 -0.8791956912476344 1105 76-77 -0.9453812215594297 1105 78-79 -0.9321292337406497 1105 80-81 -0.5693591317049034 1105 82-83 -0.9002236643636543 1105 84-85 -0.6049102217791713 1105 86-87 -0.6797768950922851 1105 88-89 -0.5256420887520079 1105 90-91 -0.4992051281352552 1105 92-93 -0.48469740850909204 1105 94-95 -0.8451763150914289 1105 96-97 -1.0131272967053881 1105 98-99 -1.0885965754652105 1105 100-101 -0.5832996200956977 1106 1 0.5254452257039297 1106 2 0.2909653238924115 1106 3 0.6460018322765464 1106 4 0.6711342946360759 1106 5 0.5639970986169871 1106 6 0.5437270958618186 1106 7 0.3449200922615816 1106 8 0.3990751623278257 1106 9 0.6511988742487276 1106 10-11 0.5571224702735336 1106 12-13 0.7778747968338919 1106 14-15 0.9073211145750406 1106 16-17 0.9071201200409433 1106 18-19 0.839650070764101 1106 20-21 0.9757958452208655 1106 22-23 0.8478913107533117 1106 24-25 0.47193211273896196 1106 26-27 0.6063316250664244 1106 28-29 0.5952946931781256 1106 30-31 0.5203727031369283 1106 32-33 0.6680791826521428 1106 34-35 0.6048474984811847 1106 36-37 0.7128176299740616 1106 38-39 1.0668752582535532 1106 40-41 1.2376252968096253 1106 42-43 1.1747866054762923 1106 44-45 0.9572002832365598 1106 46-47 1.024472814397292 1106 48-49 1.250855847449408 1106 50-51 0.9886941358092152 1106 52-53 0.980555859244113 1106 54-55 0.8490900423000163 1106 56-57 1.1142726851425557 1106 58-59 1.2847699740225913 1106 60-61 1.3806891801843086 1106 62-63 1.6189817714244086 1106 64-65 1.7083231843389264 1106 66-67 1.8196633591480875 1106 68-69 1.8644288473503003 1106 70-71 1.664852146429201 1106 72-73 1.4736414211482902 1106 74-75 1.4147914826758914 1106 76-77 1.7454741716610727 1106 78-79 1.4888553846112522 1106 80-81 1.5737552903983776 1106 82-83 1.5533131503332918 1106 84-85 1.697705622764925 1106 86-87 1.5598344651468743 1106 88-89 1.6437947211231076 1106 90-91 1.5018243332659686 1106 92-93 1.733673776215337 1106 94-95 1.3953126188012384 1106 96-97 1.408590240302182 1106 98-99 1.4912674488368296 1106 100-101 1.573819816714181 1107 1 -0.09555582101792837 1107 2 0.1393809676263551 1107 3 -0.03459575074752408 1107 4 -0.022561585086066316 1107 5 0.009543772896172698 1107 6 -0.12273974208660832 1107 7 0.013023622568937299 1107 8 -0.1753800665236298 1107 9 0.07074402602339802 1107 10-11 -0.00354933484588571 1107 12-13 -0.102620492917751 1107 14-15 -0.05782780539831833 1107 16-17 0.32165290146637915 1107 18-19 0.26321796495003724 1107 20-21 -0.08956004021066377 1107 22-23 0.1674245679614188 1107 24-25 -0.1321453446784986 1107 26-27 0.19932810427471992 1107 28-29 -0.017480071309456946 1107 30-31 -0.06554413005367365 1107 32-33 0.0719163698250398 1107 34-35 0.4764813361441398 1107 36-37 0.22581601231300397 1107 38-39 0.1683073640676227 1107 40-41 0.427740911996608 1107 42-43 0.6407300824959847 1107 44-45 1.0834063922859372 1107 46-47 1.0957046157104529 1107 48-49 1.1841915596960533 1107 50-51 1.1815455013054539 1107 52-53 1.0142008302213732 1107 54-55 1.137109834858773 1107 56-57 0.8367629315979705 1107 58-59 0.8461054959561736 1107 60-61 0.766584603155092 1107 62-63 0.7044513689122773 1107 64-65 0.683170933886931 1107 66-67 0.7344792312577084 1107 68-69 0.5749222339712965 1107 70-71 0.5279100016009615 1107 72-73 0.7555545432341191 1107 74-75 0.5744284605044214 1107 76-77 0.5001225682735004 1107 78-79 0.5860411301626804 1107 80-81 0.7450085019313519 1107 82-83 0.7220827763681186 1107 84-85 1.1097262717324767 1107 86-87 0.5375630787161967 1107 88-89 0.48707666992889287 1107 90-91 0.3337445920917368 1107 92-93 0.2332527085590428 1107 94-95 0.5050756790353219 1107 96-97 0.36479444622091606 1107 98-99 0.6305837491508477 1107 100-101 0.7664166384859961 1108 1 0.24443637054896428 1108 2 0.11931763255781647 1108 3 -0.008394119652610499 1108 4 0.27758590753930434 1108 5 0.04383580829440348 1108 6 0.21966004968851394 1108 7 0.11019523457657243 1108 8 0.37413841343472143 1108 9 0.3030015307952141 1108 10-11 0.58216990860371 1108 12-13 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-0.7234988681864571 2316 88-89 -0.7448626572292625 2316 90-91 -1.1102483833567653 2316 92-93 -1.0417937774435053 2316 94-95 -0.9984365967127573 2316 96-97 -1.2148773970351705 2316 98-99 -1.6846645205368347 2316 100-101 -1.6728330429987999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4906.0 3 964.0 4 761.0 5 719.0 6 841.0 7 979.0 8 1292.0 9 1483.0 10 1862.0 11 2850.0 12 4263.0 13 5723.0 14 6118.0 15 7515.0 16 8977.0 17 11342.0 18 9433.0 19 5424.0 20 4813.0 21 4925.0 22 5039.0 23 5308.0 24 5495.0 25 6054.0 26 6874.0 27 7771.0 28 8790.0 29 9987.0 30 11701.0 31 13757.0 32 16525.0 33 20231.0 34 26068.0 35 36570.0 36 49263.0 37 67157.0 38 60346.0 39 9384.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.68391413350743 19.4518649781159 14.95935175994333 19.90486912843334 2 16.5728293180709 22.13607524734904 39.11775145303696 22.173343981543102 3 19.387813970000888 27.383287476701874 31.61356172894293 21.61533682435431 4 12.840796660026355 18.294208730605924 36.78119357028453 32.08380103908319 5 12.344393734905527 38.27736539281148 33.19141568404603 16.186825188236966 6 28.362249347087577 36.64142293858296 18.052113172965765 16.944214541363696 7 24.598595723238052 36.571131745845 21.66084696135908 17.169425569557873 8 25.965448959295305 32.669425303686864 20.832519357817716 20.532606379200114 9 27.250324944659592 16.18557112628259 21.81188254969546 34.752221379362354 10-11 23.93709681356504 27.762749401392277 26.688251310945365 21.611902474097317 12-13 26.33511466983875 26.553934993053673 27.658578156139175 19.452372180968403 14-15 23.28913755561447 26.64214509067474 28.76611585557265 21.302601498138145 16-17 19.648423402164315 29.950195166099398 29.272138720620976 21.129242711115307 18-19 19.240079243963752 27.053045731758075 34.300094892981804 19.406780131296372 20-21 20.866918819003956 26.064260013513707 34.25958080875884 18.809240358723496 22-23 20.421755085458795 26.510208657545437 33.96618056125972 19.10185569573605 24-25 19.816763223901802 26.771991742691615 34.425539943619455 18.985705089787132 26-27 19.612949386116263 26.925781783801376 34.338604293575415 19.122664536506946 28-29 19.248699051987938 27.076268597864058 34.50874981256352 19.166282537584486 30-31 19.499294859693737 27.24373424538883 34.15577493253973 19.101195962377712 32-33 19.106319291818615 27.489180130714892 33.833434477800544 19.571066099665945 34-35 19.503264749154365 27.23709768467279 33.925944199236376 19.33369336693647 36-37 19.70257289231689 27.187825474157144 33.345671478543295 19.763930154982663 38-39 19.5796843743054 27.079239831073572 33.56779284285397 19.77328295176706 40-41 19.63158491144573 27.08209739525355 33.52241797259495 19.76389972070577 42-43 19.44611137782045 27.28058711616081 33.001169075941036 20.2721324300777 44-45 19.40254217980193 27.46441070529541 32.76571246939526 20.367334645507395 46-47 19.66003474340892 27.597336560936004 31.85179257540242 20.89083612025265 48-49 19.95104229660987 27.664659741738113 31.246998505922946 21.137299455729075 50-51 20.018429880861603 27.906263269403063 30.715430634508117 21.359876215227217 52-53 20.02076395342116 28.042452725324463 30.50444846582396 21.43233485543042 54-55 19.713873182530058 27.837842944809825 30.606521717383355 21.84176215527676 56-57 19.89500752327548 28.185501525769162 30.20211718529483 21.717373765660525 58-59 19.793462803306372 27.624211181228336 30.49584481379433 22.086481201670964 60-61 19.799610372012538 27.53741339440615 30.7620625848285 21.900913648752812 62-63 19.935451174948653 27.233536048651676 30.896081726280972 21.934931050118696 64-65 19.748187841864187 27.301996709209764 30.804353626539775 22.145461822386267 66-67 19.685618922458744 27.550927377481884 30.259679036888297 22.503774663171072 68-69 19.75265265187356 27.409457372443107 30.18180241343232 22.65608756225101 70-71 19.6491372418647 27.34318972006364 30.065166897751013 22.942506140320642 72-73 19.75162737435447 27.24049563346693 29.717328826900737 23.290548165277862 74-75 19.493236332589227 27.17640231592241 29.68876457456932 23.64159677691904 76-77 19.57755527594624 26.95794448618824 29.81188616245104 23.652614075414487 78-79 19.502399767079794 26.881280705517074 29.862125908038745 23.75419361936438 80-81 19.437628084588063 27.046463865142766 29.577148598126684 23.938759452142484 82-83 19.519823587796527 26.730583487364274 29.495026736363123 24.254566188476076 84-85 19.390861590068656 26.380770414928584 29.55306064152709 24.67530735347567 86-87 19.47033469743414 26.180954900204284 29.690643276153722 24.658067126207854 88-89 19.74236905316348 25.806777901404864 29.72970870074106 24.7211443446906 90-91 19.654818565175674 25.785657982654207 29.884520756135007 24.675002696035104 92-93 19.856961482104065 25.521246767569632 29.85375962502696 24.768032125299342 94-95 19.803243983686684 25.141763065551242 29.904890881354877 25.150102069407197 96-97 19.97562768170553 24.67652752281847 29.96082861434698 25.38701618112902 98-99 20.02870774030502 24.463842367599167 30.02471623032458 25.482733661771228 100-101 20.570280838803907 23.868519510660672 30.61260784605774 24.948591804477683 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1333.0 1 919.5 2 590.5 3 626.0 4 526.0 5 557.0 6 788.5 7 1287.0 8 1918.0 9 2161.0 10 1908.0 11 1630.5 12 1568.5 13 1608.0 14 1669.0 15 1711.0 16 1788.5 17 1893.0 18 2006.5 19 2127.0 20 2301.5 21 2601.0 22 2974.0 23 3289.5 24 3604.0 25 4131.0 26 4823.5 27 5522.0 28 6146.0 29 6732.5 30 7421.0 31 8013.0 32 8618.0 33 9203.5 34 9781.0 35 10251.0 36 10679.0 37 11324.5 38 11511.0 39 11292.0 40 11289.0 41 11664.5 42 12072.5 43 12628.5 44 13183.0 45 13532.0 46 13958.0 47 14388.5 48 15139.5 49 15277.0 50 14518.0 51 13781.5 52 12999.0 53 12537.0 54 12151.0 55 11276.5 56 10271.0 57 9300.5 58 8532.5 59 7810.0 60 6985.0 61 6146.0 62 5154.5 63 4017.0 64 3041.0 65 2417.5 66 1924.0 67 1546.0 68 1221.0 69 925.0 70 737.5 71 600.0 72 489.0 73 411.0 74 336.0 75 272.5 76 217.0 77 164.0 78 115.0 79 78.0 80 51.5 81 44.0 82 39.0 83 26.5 84 18.5 85 14.0 86 10.5 87 7.5 88 8.5 89 6.5 90 2.0 91 1.0 92 1.0 93 2.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2628932879157679 2 0.16256417298245457 3 0.1847118804732522 4 0.16212121883263864 5 0.22546366225631984 6 0.18626221999760803 7 0.22878581837993947 8 0.2325509286533751 9 0.14794668603852815 10-11 0.196007211293559 12-13 0.20287300061570626 14-15 0.16710445301806812 16-17 0.2782859446218722 18-19 0.22291667589487812 20-21 0.19058102295831358 22-23 0.2354301306271788 24-25 0.22236298320760817 26-27 0.3254605615772711 28-29 0.3015410374872097 30-31 0.2766248665600624 32-33 0.1857085273103381 34-35 0.3446183285568111 36-37 0.37418551805702593 38-39 0.3574639989014737 40-41 0.32136323569147357 42-43 0.3512626408040504 44-45 0.3597895081880075 46-47 0.364219049686167 48-49 0.38492715619006274 50-51 0.3767325044184677 52-53 0.37562511904392776 54-55 0.3717492702330382 56-57 0.34926934712987856 58-59 0.32645720841435705 60-61 0.2970007574515962 62-63 0.3592358155007375 64-65 0.39290033088674986 66-67 0.3997661202088972 68-69 0.4036419690197868 70-71 0.4036419690197868 72-73 0.39566879432309965 74-75 0.3893566976882223 76-77 0.37208148584540013 78-79 0.34871565444260866 80-81 0.35790695305128967 82-83 0.36731972873487867 84-85 0.36887006825923446 86-87 0.36432978822362094 88-89 0.373078132682486 90-91 0.3941184547987438 92-93 0.3931218079616579 94-95 0.4030882763325168 96-97 0.40386344609469477 98-99 0.40076276704598307 100-101 0.37274591707012406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 451514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.96663100373237 #Duplication Level Percentage of deduplicated Percentage of total 1 87.13352796417891 60.96439399123103 2 8.131854231700677 11.379168888110817 3 1.9916279710486664 4.180424980412227 4 0.8250506970267288 2.3090407071296526 5 0.4561270809566283 1.595683758205099 6 0.30898908051462354 1.297135498832953 7 0.2022424488616322 0.9905155954955124 8 0.15052669073455482 0.8425476341470031 9 0.11613328690799314 0.7312909349107926 >10 0.6310150450366796 7.923540534161212 >50 0.03706553219031429 1.7355498881241498 >100 0.012988776090937012 1.700750213650638 >500 9.503982505563664E-4 0.4707928390191521 >1k 0.0012671976674084886 1.4423017907239277 >5k 6.335988337042443E-4 2.436862745845837 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5975 1.3233255225751581 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5019 1.111593438963133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3094 0.6852500697652786 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1203 0.26643692111429546 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1155 0.25580602151871257 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.23365831402791495 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 955 0.21151060653711737 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 666 0.14750373188871221 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 503 0.11140296867871208 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 0.10608751888092063 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.214770749079763E-4 0.0 10-11 0.0 0.0 0.0 2.214770749079763E-4 0.0 12-13 0.0 0.0 0.0 2.214770749079763E-4 0.0 14-15 0.0 0.0 0.0 2.214770749079763E-4 0.0 16-17 0.0 0.0 0.0 2.214770749079763E-4 0.0 18-19 0.0 0.0 0.0 5.536926872699408E-4 0.0 20-21 0.0 0.0 0.0 0.001550339524355834 0.0 22-23 0.0 0.0 0.0 0.004318802960705538 0.0 24-25 0.0 0.0 0.0 0.011406069357760777 0.0 26-27 0.0 0.0 0.0 0.022036968953343637 0.0 28-29 0.0 0.0 0.0 0.054815576039724125 0.0 30-31 0.0 0.0 0.0 0.13089295127061398 0.0 32-33 0.0 0.0 0.0 0.2539234663819948 0.0 34-35 0.0 0.0 0.0 0.4457226132523022 0.0 36-37 0.0 0.0 0.0 0.737629397981015 0.0 38-39 0.0 0.0 0.0 1.1127008243376728 0.0 40-41 0.0 0.0 0.0 1.5125776830840238 0.0 42-43 0.0 0.0 0.0 1.8883135406654057 0.0 44-45 0.0 0.0 0.0 2.318089804524334 0.0 46-47 0.0 0.0 0.0 2.8133125440185687 0.0 48-49 0.0 0.0 0.0 3.3220453850821903 0.0 50-51 0.0 0.0 0.0 3.8341003822694315 0.0 52-53 0.0 0.0 0.0 4.298980762501274 0.0 54-55 0.0 0.0 0.0 4.829086141293515 0.0 56-57 0.0 0.0 0.0 5.4102419858520445 0.0 58-59 0.0 0.0 0.0 6.015095877425727 0.0 60-61 0.0 0.0 0.0 6.677423069938031 0.0 62-63 0.0 0.0 0.0 7.308521994888309 0.0 64-65 0.0 0.0 0.0 7.978490146484937 0.0 66-67 0.0 0.0 0.0 8.710693356130708 0.0 68-69 0.0 0.0 0.0 9.491400045181322 0.0 70-71 0.0 0.0 0.0 10.339103549391602 0.0 72-73 0.0 0.0 0.0 11.18038421842955 0.0 74-75 0.0 0.0 0.0 12.046691796932098 0.0 76-77 0.0 0.0 0.0 12.961945808989313 0.0 78-79 0.0 0.0 0.0 13.93733084688404 0.0 80-81 0.0 0.0 0.0 14.935860239106649 0.0 82-83 0.0 0.0 0.0 15.952661490009168 0.0 84-85 0.0 0.0 0.0 16.974667452172024 0.0 86-87 0.0 0.0 0.0 18.015388227164607 0.0 88-89 0.0 0.0 0.0 19.067515071514947 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3290 0.0 44.050156 1 GTATCAA 4995 0.0 32.15324 1 TACTAGC 80 4.5183362E-4 29.65489 2 TATCAAC 6095 0.0 26.312328 2 ATCAACG 6300 0.0 25.23019 3 AACGCAG 6350 0.0 25.03153 6 TCAACGC 6390 0.0 24.949091 4 CAACGCA 6385 0.0 24.894316 5 GTCTACT 115 1.2770624E-4 24.791164 1 GTACATG 3255 0.0 24.232634 1 TACATGG 3215 0.0 23.90839 2 GAGTAGG 160 1.8498031E-6 23.7582 1 CTATTAG 140 1.7716195E-5 23.7582 1 ACATGGG 3195 0.0 22.87 3 GAGTACT 4330 0.0 22.755383 12-13 CATGGGG 960 0.0 22.241167 4 ATTAGCT 150 3.053734E-5 22.142317 3 ACGCAGA 7245 0.0 21.876236 7 CGCAGAG 7235 0.0 21.840885 8 CATGGGA 1885 0.0 20.892145 4 >>END_MODULE