FastQCFastQC Report
Thu 26 May 2016
SRR938832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451514
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51781.1468082938735011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48291.0695127947306176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.7045185752822725No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT24080.5333167963784069No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT13570.30054439065012384RNA PCR Primer, Index 39 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12390.2744100958109826No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.25093352587073714No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.2259066164061358No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.21328242313638116No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG7680.1700943935293258RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT6790.1503829338625159RNA PCR Primer, Index 39 (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.11937614337539922No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.11450364772742373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCA250.00503859856.9447829
GGTATCA33550.043.3775061
GTATCAA51050.032.234141
CGACCGT350.00843039427.11656418-19
TATCAAC62250.026.6076722
ATCAACG63150.026.078163
TCAACGC63450.025.9548574
CTAATAC1109.519525E-525.886863
CAACGCA65000.025.3359365
AACGCAG65500.025.1397446
CGCAGAA1401.7897524E-523.7269949
ATGGGAT6600.023.0105425
TAGAGTG1050.002205144122.599645
GAGTACT44850.022.43583912-13
GTCCTGC1502.9988954E-522.1943511
CGCAGAG75100.021.8629558
ACGCAGA75400.021.7130327
TTAGAGC1754.0704217E-621.6956543
GTATAGG1100.002853935621.6178741
GTGGTAT18300.021.3107361
Produced by FastQC (version 0.11.5)