##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938832_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 451514 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906180096298232 31.0 31.0 34.0 30.0 34.0 2 32.08377591835469 33.0 31.0 34.0 30.0 34.0 3 32.11074296699549 34.0 31.0 34.0 30.0 34.0 4 35.810380187546784 37.0 35.0 37.0 35.0 37.0 5 35.72330426077597 37.0 35.0 37.0 33.0 37.0 6 35.7787067510642 37.0 35.0 37.0 33.0 37.0 7 35.67931891369924 37.0 35.0 37.0 33.0 37.0 8 35.69351116465935 37.0 35.0 37.0 33.0 37.0 9 37.253914607298995 39.0 37.0 39.0 33.0 39.0 10-11 37.21548501264634 39.0 37.0 39.0 33.0 39.0 12-13 37.12182457243851 39.0 37.0 39.0 33.0 39.0 14-15 38.21557028132018 40.0 37.5 41.0 33.0 41.0 16-17 38.1616616539022 40.0 37.0 41.0 33.0 41.0 18-19 38.3524043551252 40.0 38.0 41.0 33.5 41.0 20-21 38.37849103239324 40.0 38.0 41.0 34.0 41.0 22-23 38.252888061056794 40.0 37.5 41.0 33.0 41.0 24-25 38.12912002728598 40.0 37.0 41.0 33.0 41.0 26-27 37.854637951425644 40.0 37.0 41.0 33.0 41.0 28-29 37.58317239332557 40.0 36.0 41.0 32.0 41.0 30-31 37.17558481021629 39.0 35.5 41.0 32.0 41.0 32-33 36.75045402800356 39.0 35.0 41.0 31.0 41.0 34-35 36.46828891241468 39.0 35.0 41.0 30.5 41.0 36-37 36.16571800652915 39.0 35.0 41.0 30.0 41.0 38-39 35.85060485389157 39.0 35.0 41.0 30.0 41.0 40-41 35.48071931324388 39.0 35.0 41.0 28.0 41.0 42-43 35.02465039843726 38.0 35.0 41.0 25.5 41.0 44-45 34.44437492525148 38.0 34.5 40.5 23.0 41.0 46-47 33.67319064303654 38.0 33.5 40.0 12.5 41.0 48-49 32.97691655186772 38.0 33.0 40.0 2.0 41.0 50-51 32.518146724132585 38.0 33.0 40.0 2.0 41.0 52-53 32.19732942943076 37.5 32.5 40.0 2.0 41.0 54-55 31.841619528962557 37.0 32.0 40.0 2.0 41.0 56-57 31.456026834162397 37.0 31.0 40.0 2.0 41.0 58-59 31.043812594958297 36.0 31.0 40.0 2.0 41.0 60-61 30.59324738546313 35.5 30.0 39.5 2.0 41.0 62-63 30.139597000314495 35.0 29.0 39.0 2.0 41.0 64-65 29.67766226517893 35.0 28.5 39.0 2.0 40.0 66-67 29.20177115216804 35.0 28.0 38.0 2.0 40.0 68-69 28.837070389843948 35.0 27.5 38.0 2.0 40.0 70-71 28.42144429630089 35.0 26.0 37.0 2.0 39.5 72-73 27.95669569492862 34.0 26.0 36.5 2.0 39.0 74-75 27.4927233707039 34.0 25.5 36.0 2.0 39.0 76-77 25.732704855220437 31.5 22.0 34.5 2.0 36.5 78-79 26.549680630057985 33.5 23.5 35.0 2.0 37.0 80-81 26.571999982281834 34.0 24.0 35.0 2.0 37.0 82-83 26.261335196693793 34.0 22.5 35.0 2.0 36.5 84-85 25.889100891666704 33.0 20.0 35.0 2.0 36.0 86-87 25.362249011104858 33.0 17.5 35.0 2.0 35.5 88-89 24.8154597642598 32.5 9.0 35.0 2.0 35.0 90-91 24.329723995269248 32.0 4.5 35.0 2.0 35.0 92-93 24.04045500250269 32.0 2.0 35.0 2.0 35.0 94-95 23.722792648732927 32.0 2.0 34.5 2.0 35.0 96-97 23.17447631745638 31.5 2.0 34.0 2.0 35.0 98-99 22.44430405258752 31.0 2.0 34.0 2.0 35.0 100-101 19.517255278906077 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.22750263928557857 1101 2 -0.017735397187450985 1101 3 0.04155123656413906 1101 4 0.015250591723280138 1101 5 0.057832590272532514 1101 6 -6.148454023531258E-4 1101 7 0.2532288378282743 1101 8 0.09775364633549799 1101 9 0.22806169914408514 1101 10-11 0.014322519082234919 1101 12-13 -0.0010771166638008367 1101 14-15 0.22836563664886 1101 16-17 0.2674998886193052 1101 18-19 0.17733312963788705 1101 20-21 0.31695447719837944 1101 22-23 0.3673747331893935 1101 24-25 0.3201490934106772 1101 26-27 0.19368010998253737 1101 28-29 0.1881839077632037 1101 30-31 0.1609320451411662 1101 32-33 0.11096540480023265 1101 34-35 0.2815644639760322 1101 36-37 0.4722704234615591 1101 38-39 0.28099906874285807 1101 40-41 0.5409329612291103 1101 42-43 0.7974378098556514 1101 44-45 0.5797738151325049 1101 46-47 0.5001941251838247 1101 48-49 0.50607722558901 1101 50-51 0.20009418080286423 1101 52-53 -0.031063111378252017 1101 54-55 0.1349901391509185 1101 56-57 0.3122383158530475 1101 58-59 0.2817735793956615 1101 60-61 0.2152135820531207 1101 62-63 -0.13444391222133234 1101 64-65 0.02896608266715006 1101 66-67 0.23088180167777494 1101 68-69 -0.06669665875635644 1101 70-71 -0.02643112366552458 1101 72-73 -0.0689627426889281 1101 74-75 -0.48301735543804014 1101 76-77 -0.5628903815979633 1101 78-79 -0.602193322679689 1101 80-81 -0.39987815443279473 1101 82-83 -0.5119384893542964 1101 84-85 -0.49539853070974615 1101 86-87 -0.3300863708757511 1101 88-89 -0.750532672662036 1101 90-91 -0.7401669006778846 1101 92-93 -0.7739844812138124 1101 94-95 -0.41872625591703994 1101 96-97 -0.7056875148875683 1101 98-99 -1.0868952594742005 1101 100-101 -0.27089432316997986 1102 1 0.19724362535299633 1102 2 0.10566748341559418 1102 3 0.07789529363776637 1102 4 0.024479290067610293 1102 5 -0.08071095529214745 1102 6 -0.07959721180429113 1102 7 0.11022708792922487 1102 8 0.017737493421243755 1102 9 0.11100152953848408 1102 10-11 0.1567777620489963 1102 12-13 0.21802908874742855 1102 14-15 0.13820276143012222 1102 16-17 0.49851079183643066 1102 18-19 0.24557650021265687 1102 20-21 0.21575377723876699 1102 22-23 0.10046936234540738 1102 24-25 0.24044523017202124 1102 26-27 0.21965399610449055 1102 28-29 0.3958700027961797 1102 30-31 0.14127933279764449 1102 32-33 0.03774424448255331 1102 34-35 0.25358492433408486 1102 36-37 -0.06229142873927174 1102 38-39 0.027934053262981706 1102 40-41 0.04468312948863229 1102 42-43 0.15696937534225697 1102 44-45 -0.06310948006200334 1102 46-47 -0.05355200484713407 1102 48-49 -0.26214729991913543 1102 50-51 -0.017945932267537046 1102 52-53 -0.29995798282797637 1102 54-55 -0.16185196611224129 1102 56-57 -0.1172972878621259 1102 58-59 0.05173723533858521 1102 60-61 -0.14225848686598042 1102 62-63 -0.3667470485788513 1102 64-65 -0.03540058848643923 1102 66-67 0.31686914856160797 1102 68-69 0.31246608185476177 1102 70-71 -0.03219771405453997 1102 72-73 0.1976464913937157 1102 74-75 0.13460008970935533 1102 76-77 -0.2525659772366744 1102 78-79 -0.03676863564240307 1102 80-81 0.3538518226839109 1102 82-83 0.19208897059993646 1102 84-85 0.26078399722133483 1102 86-87 0.40571656357033703 1102 88-89 0.13879563532019645 1102 90-91 0.5607376625203919 1102 92-93 0.255820337738939 1102 94-95 0.47941615894364276 1102 96-97 0.5468632994885247 1102 98-99 0.3565749249382364 1102 100-101 0.8373598160142954 1103 1 0.062258306188500256 1103 2 0.1599761257779022 1103 3 0.13448704102290776 1103 4 0.01571258473966708 1103 5 -0.139288011392793 1103 6 0.11740288223665374 1103 7 0.24535346850245787 1103 8 0.07310148410074646 1103 9 0.14891047701139115 1103 10-11 0.15126853036345977 1103 12-13 0.25030080436763313 1103 14-15 0.2868292412687836 1103 16-17 0.15788667347023022 1103 18-19 0.08433286103728932 1103 20-21 0.2953751057237639 1103 22-23 0.5014520901595816 1103 24-25 0.5043580646704129 1103 26-27 0.2514346090424411 1103 28-29 0.19833701010002613 1103 30-31 0.526295998941869 1103 32-33 0.5141295197612763 1103 34-35 0.4230310232024621 1103 36-37 0.5458347533030903 1103 38-39 0.2975206910904262 1103 40-41 0.6335518169905967 1103 42-43 0.2739406301618601 1103 44-45 0.16525326718728905 1103 46-47 0.15823602687601124 1103 48-49 0.2736222161879951 1103 50-51 0.08571330516494413 1103 52-53 -0.12525865260844427 1103 54-55 -0.11564913025547341 1103 56-57 -0.3088978646465499 1103 58-59 -0.022894698874551977 1103 60-61 -0.10352668117545605 1103 62-63 0.08678627848508569 1103 64-65 0.4572067487966116 1103 66-67 0.6476424785512656 1103 68-69 0.30735652416064596 1103 70-71 0.4245277804440626 1103 72-73 0.7108331208619703 1103 74-75 0.4697384866248093 1103 76-77 0.40361512471415395 1103 78-79 0.3123191672477894 1103 80-81 0.6449163661151509 1103 82-83 0.8507099124721869 1103 84-85 0.8401937335932352 1103 86-87 0.36723030922094324 1103 88-89 0.07590836950825874 1103 90-91 0.2685733625506934 1103 92-93 0.4295583605804403 1103 94-95 0.43531618297632235 1103 96-97 0.5986772447149029 1103 98-99 0.44808459548147894 1103 100-101 1.1045797568729938 1104 1 0.14365125793922573 1104 2 0.012491664291772508 1104 3 0.03438087406247803 1104 4 0.06728086658717558 1104 5 -0.0715473061555656 1104 6 -0.044120355958717994 1104 7 0.07210548103634551 1104 8 0.06851981284724928 1104 9 -0.038829815926959554 1104 10-11 -0.07793614510578095 1104 12-13 -0.11084208811512752 1104 14-15 -0.019606744177060875 1104 16-17 0.1742214395421584 1104 18-19 0.2551740538179388 1104 20-21 0.23549384779989424 1104 22-23 0.2613781855861603 1104 24-25 0.16609518290742642 1104 26-27 0.1801945170997854 1104 28-29 0.16874425831426265 1104 30-31 0.0043127382762051525 1104 32-33 0.2554428978290417 1104 34-35 -0.05159713474923677 1104 36-37 0.4414458317738692 1104 38-39 0.15315681671789605 1104 40-41 0.19715922600138924 1104 42-43 0.06306521657455022 1104 44-45 0.05571060971489317 1104 46-47 0.29651333909963995 1104 48-49 0.7401152019533654 1104 50-51 0.5281409209436134 1104 52-53 0.8492741612283083 1104 54-55 0.6946289653360118 1104 56-57 1.030498839023938 1104 58-59 0.8561780409545463 1104 60-61 0.5446200353793742 1104 62-63 0.6499246975250479 1104 64-65 0.8157779270437224 1104 66-67 0.5127434402383919 1104 68-69 0.6342992249244297 1104 70-71 0.2968645873954223 1104 72-73 0.6280177379909269 1104 74-75 0.6159628596728339 1104 76-77 0.6084812821917076 1104 78-79 0.42237977518248115 1104 80-81 0.4270292947771388 1104 82-83 0.7244949192235808 1104 84-85 0.8052282120866998 1104 86-87 0.6514459621477542 1104 88-89 0.6167465731220538 1104 90-91 0.8185079101042163 1104 92-93 0.5460765996519399 1104 94-95 0.5236718319862916 1104 96-97 0.4422742431790887 1104 98-99 0.8825058824724188 1104 100-101 1.5726961694604817 1105 1 0.026588629508935924 1105 2 -0.00176599597404703 1105 3 0.21261428061583842 1105 4 0.1218820873708708 1105 5 -0.05136287266600448 1105 6 0.11451572700598689 1105 7 0.08481944716617562 1105 8 0.13936272045842202 1105 9 0.13664518635062706 1105 10-11 0.1103262120392472 1105 12-13 -0.0012195592910657638 1105 14-15 0.19400003390551745 1105 16-17 0.28160434654597566 1105 18-19 0.2845396714474333 1105 20-21 0.32756381510395016 1105 22-23 0.25332566961778014 1105 24-25 0.1773160678237673 1105 26-27 0.329024346331785 1105 28-29 0.3237286189212085 1105 30-31 0.07790645822478126 1105 32-33 -0.13846482469067212 1105 34-35 -0.05256637221494742 1105 36-37 0.12993594707010914 1105 38-39 0.31540160105623727 1105 40-41 0.3021278966023644 1105 42-43 0.07535656480158082 1105 44-45 -0.06040555706481143 1105 46-47 -0.5359098051775533 1105 48-49 -0.9123638189902579 1105 50-51 -0.9291012821356688 1105 52-53 -0.8466737681970322 1105 54-55 -0.5494493227324888 1105 56-57 -0.7984396055397269 1105 58-59 -0.7760634029309017 1105 60-61 -0.9703284912784511 1105 62-63 -0.9893291667715864 1105 64-65 -1.00544963877838 1105 66-67 -0.8997037957899146 1105 68-69 -0.9187937307690177 1105 70-71 -0.7431575810646294 1105 72-73 -0.2979102499429551 1105 74-75 -0.29451300302443073 1105 76-77 -0.5313419774829526 1105 78-79 -0.5914679204111586 1105 80-81 -0.4793848808901906 1105 82-83 -0.4317142741076587 1105 84-85 -0.47344653915078894 1105 86-87 -0.5577650836164523 1105 88-89 -0.44618553470371225 1105 90-91 -0.258122585193842 1105 92-93 -0.5762978549938182 1105 94-95 -0.13233744030110728 1105 96-97 -0.29562420705322623 1105 98-99 0.016285959201606914 1105 100-101 0.4256580376846735 1106 1 0.2908781323779266 1106 2 0.08640122520344562 1106 3 0.1661559171460496 1106 4 0.2276731091173403 1106 5 0.1614632038082675 1106 6 0.1354762361915789 1106 7 0.321388230617643 1106 8 0.09942347555751496 1106 9 0.2398517054691922 1106 10-11 0.12330417626123591 1106 12-13 0.15734160566384503 1106 14-15 0.30219232861596623 1106 16-17 0.24170608722778297 1106 18-19 0.04774426183269043 1106 20-21 0.0888203848517648 1106 22-23 0.29972134925517935 1106 24-25 0.17942803079208147 1106 26-27 0.34386353819252946 1106 28-29 0.3054514599453668 1106 30-31 0.48718125450232463 1106 32-33 0.5289034187154726 1106 34-35 0.5559224652410535 1106 36-37 0.7410623273198738 1106 38-39 0.7660212066302705 1106 40-41 0.950888685454288 1106 42-43 0.6807015669714076 1106 44-45 0.8737523097561493 1106 46-47 1.2031542769864814 1106 48-49 1.2199545184416323 1106 50-51 1.4729721061665586 1106 52-53 1.5667357501018202 1106 54-55 1.418000891839089 1106 56-57 1.4627253493761323 1106 58-59 1.8156382220269052 1106 60-61 1.597541222786834 1106 62-63 1.8131082699418535 1106 64-65 1.7330647543939044 1106 66-67 1.7289210173640512 1106 68-69 1.5701787365630615 1106 70-71 1.683461349452756 1106 72-73 1.717476219992914 1106 74-75 1.912314712873151 1106 76-77 1.7913158170105135 1106 78-79 1.984119137788369 1106 80-81 1.6530250713736585 1106 82-83 1.756233553656454 1106 84-85 1.6443990657481926 1106 86-87 1.7358339327295624 1106 88-89 1.8060011666801508 1106 90-91 1.879871049986253 1106 92-93 2.19393455736936 1106 94-95 2.599110556227174 1106 96-97 2.585457330147218 1106 98-99 2.629208472341105 1106 100-101 2.32270466481231 1107 1 -0.09668015099822824 1107 2 -0.06511176366323923 1107 3 -0.09288395344106704 1107 4 -0.0808243692345556 1107 5 -0.2883464831267375 1107 6 -0.3046179687755952 1107 7 -0.042913796216495825 1107 8 -0.09345405798516282 1107 9 -0.19195912334420484 1107 10-11 -0.2562657162118782 1107 12-13 -0.01974708022444105 1107 14-15 -0.268930042684822 1107 16-17 -0.2822424330881361 1107 18-19 -0.3028100249690908 1107 20-21 -0.27293620473810876 1107 22-23 -0.07993656301049867 1107 24-25 -0.03962706484023215 1107 26-27 -0.11264992645681815 1107 28-29 -0.2342002671456953 1107 30-31 -0.4448722234689342 1107 32-33 -0.03780606839081457 1107 34-35 -0.4128733288402131 1107 36-37 -0.5293511278138183 1107 38-39 -0.47608318008587247 1107 40-41 -0.3726459039452834 1107 42-43 -0.12953251809854294 1107 44-45 -0.2725270698232478 1107 46-47 -0.33574809241117265 1107 48-49 0.143838808098117 1107 50-51 0.036348735722185666 1107 52-53 0.2106052904957707 1107 54-55 0.38361845773498615 1107 56-57 0.23930494781556533 1107 58-59 0.2821978375945129 1107 60-61 0.2359892197418283 1107 62-63 0.2797440107354596 1107 64-65 0.07506446892430674 1107 66-67 0.31726909995991903 1107 68-69 0.01917007731860565 1107 70-71 -0.06275855729384361 1107 72-73 0.22560258786807097 1107 74-75 0.47831528381234634 1107 76-77 0.2454646418273363 1107 78-79 0.23758283557122084 1107 80-81 0.1144581287530464 1107 82-83 0.1824420416191579 1107 84-85 -0.10155678162072945 1107 86-87 0.005595565615660547 1107 88-89 0.22691657252276443 1107 90-91 0.08613710765110838 1107 92-93 0.3641362892182549 1107 94-95 0.09836119853376601 1107 96-97 0.19380493696042578 1107 98-99 0.27851858748050873 1107 100-101 1.1064638236285624 1108 1 0.22318363522422402 1108 2 0.1207940145772568 1108 3 0.13959045225040967 1108 4 0.05476039564484836 1108 5 -0.041383096001979425 1108 6 0.06428711538196552 1108 7 0.2048955044949352 1108 8 0.0392242345707956 1108 9 0.05580679718330117 1108 10-11 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-1.3029431275056709 2316 52-53 -1.0656980392793756 2316 54-55 -0.9969942966467507 2316 56-57 -0.6813460283904895 2316 58-59 -0.584520767136727 2316 60-61 -0.6388170885808897 2316 62-63 -0.8845194443797233 2316 64-65 -0.7364218779645775 2316 66-67 -0.7392372186930203 2316 68-69 -0.7786330286350491 2316 70-71 -0.5885712014865376 2316 72-73 -0.6024852878937814 2316 74-75 -0.6905408161842068 2316 76-77 -0.6915468247199605 2316 78-79 -0.2958440436684775 2316 80-81 -0.7746641466506894 2316 82-83 -0.4496427695099392 2316 84-85 -0.9159226781118939 2316 86-87 -0.8607959153939504 2316 88-89 -1.1577723156009263 2316 90-91 -1.6825175530010732 2316 92-93 -1.289921766633821 2316 94-95 -1.7497974392541877 2316 96-97 -1.885087835327031 2316 98-99 -1.7975979374325455 2316 100-101 -1.9850920800937608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 9.0 5 36.0 6 104.0 7 279.0 8 519.0 9 980.0 10 1635.0 11 2609.0 12 3751.0 13 5220.0 14 5668.0 15 7219.0 16 8966.0 17 11383.0 18 10333.0 19 5975.0 20 4938.0 21 4831.0 22 4940.0 23 5216.0 24 5708.0 25 6253.0 26 6785.0 27 7089.0 28 8658.0 29 10027.0 30 12009.0 31 13928.0 32 16765.0 33 20764.0 34 27353.0 35 37973.0 36 52796.0 37 75783.0 38 60124.0 39 4886.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.707827260458835 20.468206193621707 15.573282903615313 20.25068364230414 2 16.509565594865276 22.7383868495772 38.55273590630634 22.199311649251186 3 19.20117648622191 27.45385525144292 32.28249843858662 21.062469823748543 4 12.986751241379006 19.29397538060835 35.45737230739246 32.26190107062018 5 12.141398209150964 39.06616216886793 33.380150020159235 15.412289601821863 6 28.32592835405694 37.096952810422195 17.439944362987212 17.137174472533655 7 24.278759905562175 36.83606709869461 20.88750293457124 17.99767006117197 8 26.20981852168482 33.561750023255094 20.366588854387683 19.861842600672404 9 26.16840230867703 16.407907617482515 21.298342908525537 36.12534716531492 10-11 23.869248794057327 28.070558166524183 26.409812320326726 21.650380719091768 12-13 26.10439543402862 26.536497207174087 27.44311361330989 19.915993745487405 14-15 22.950008194651772 27.02330381782182 28.124930788414094 21.90175719911232 16-17 19.808149971705333 30.345947993182705 28.74151298398555 21.104389051126415 18-19 19.25455246127473 27.29328437213464 33.52044454878475 19.93171861780587 20-21 20.67865005293302 26.300956338009453 33.7451330412789 19.27526056777863 22-23 20.17434675336756 27.145669901708473 33.28412850985794 19.39585483506602 24-25 19.68166923766061 27.363644115376907 33.94055300917031 19.01413363779217 26-27 19.382688159182823 27.52366524475865 33.90608319712251 19.187563398936007 28-29 18.83961516143464 27.197938491386758 34.25109741890617 19.711348928272436 30-31 19.05145798358412 27.309895152752738 34.10979504511488 19.52885181854826 32-33 18.744158542149304 27.776658088121298 33.7549887711123 19.7241945986171 34-35 19.10126296297937 27.864259166559613 33.9046420866576 19.12983578380342 36-37 19.470851650390376 27.509644491259316 33.030734997181135 19.988768861169167 38-39 19.41541116258773 27.27461011351315 33.361059086866526 19.9489196370326 40-41 19.382397338480946 27.265508454022015 33.46534609604371 19.886748111453326 42-43 19.577958322996775 27.337797249884822 33.05055463018747 20.03368979693093 44-45 19.253880433049243 27.69337934333859 32.60051030352428 20.452229920087888 46-47 19.81488843584272 27.53562201627201 31.479486126045515 21.170003421839752 48-49 19.909901130444027 27.598758814982816 31.015672064920796 21.47566798965236 50-51 19.723197951780012 28.016296283171727 30.59307131118858 21.66743445385968 52-53 19.853869425975716 28.243863977639677 30.587866599928244 21.314399996456366 54-55 19.447791209131942 28.059041358628967 30.286768516590847 22.20639891564824 56-57 19.873358006152703 28.557791760518615 29.463975247448005 22.10487498588068 58-59 19.343139654250223 28.381447648620245 30.128689966922217 22.146722730207316 60-61 19.669791052974293 28.68160052447165 29.70972443089953 21.938883991654524 62-63 19.42036785412521 28.796561763236955 29.609721147127253 22.17334923551058 64-65 19.22769404547015 28.646526616538022 29.587158503228096 22.538620834763734 66-67 19.165884986084794 28.688803270101186 29.12846398935069 23.01684775446333 68-69 19.24795077882586 28.356547873483155 29.073360013997384 23.3221413336936 70-71 19.10948497721001 28.122383802052646 28.837533276930504 23.930597943806838 72-73 19.08755874679412 27.925490682459458 28.529015711583693 24.457934859162727 74-75 18.709891752720022 27.909969269953766 28.441737493424878 24.93840148390133 76-77 18.543251816863286 27.61986754820131 28.906602313308227 24.930278321627185 78-79 18.48203226618438 27.480702719305928 28.623846903619494 25.413418110890195 80-81 18.291813212611956 27.533952886770557 28.508354226281707 25.665879674335777 82-83 18.27056070678312 27.139346704007988 28.449283017781426 26.140809571427464 84-85 18.190908809029178 26.86782691123642 28.403991902798143 26.53727237693626 86-87 18.06491018813712 26.743028550905212 28.456681461643175 26.735379799314497 88-89 18.15138900310495 26.34071749417128 28.43505724261936 27.072836260104417 90-91 17.794875728580593 26.35452345298564 28.394382355982007 27.456218462451766 92-93 17.793325537305762 26.09244051099417 28.521501089672036 27.59273286202803 94-95 17.6000274631877 25.63212395642655 28.74266217953616 28.025186400849588 96-97 17.650413553526086 25.337559120602155 28.780867017265265 28.231160308606494 98-99 17.50366544558973 25.20320521622807 28.713173899369675 28.579955438812526 100-101 17.517571612557433 24.68962447080294 29.432492561064606 28.360311355575018 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 984.0 1 693.0 2 446.0 3 490.0 4 453.0 5 482.0 6 745.5 7 1199.0 8 1657.0 9 1898.5 10 1664.0 11 1379.5 12 1369.5 13 1420.0 14 1499.0 15 1605.0 16 1670.0 17 1713.0 18 1782.0 19 1890.0 20 2130.0 21 2312.5 22 2523.5 23 2890.0 24 3248.5 25 3579.5 26 4109.5 27 4788.0 28 5436.5 29 6137.0 30 6910.5 31 7739.0 32 8531.5 33 9244.5 34 10085.0 35 10861.5 36 11471.5 37 12080.0 38 12246.0 39 12002.5 40 11945.5 41 12285.0 42 12707.0 43 13359.5 44 14165.0 45 14501.5 46 14555.5 47 14850.0 48 15465.0 49 15748.0 50 15378.5 51 14610.0 52 13409.0 53 12582.0 54 12005.0 55 11083.0 56 10195.5 57 9187.5 58 8440.5 59 7809.0 60 6906.0 61 5951.0 62 5022.0 63 3932.5 64 2881.5 65 2178.5 66 1683.5 67 1360.5 68 1058.5 69 768.5 70 593.5 71 482.0 72 376.5 73 306.0 74 234.5 75 182.0 76 153.0 77 103.0 78 60.0 79 37.0 80 26.0 81 19.0 82 15.0 83 11.0 84 5.5 85 2.5 86 3.0 87 2.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21837639585926463 2 0.0 3 0.0 4 0.0 5 0.02391952409006144 6 0.00354363319852762 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003432894661073632 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.536926872699407E-4 26-27 0.00177181659926381 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007973174696687145 36-37 0.020043675279171853 38-39 0.015171179631196376 40-41 0.009412775683588991 42-43 0.009302037146135003 44-45 0.0039865873483435725 46-47 5.536926872699407E-4 48-49 0.0022147707490797626 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011073853745398813 58-59 5.536926872699407E-4 60-61 0.0026577248988957154 62-63 7.75169762177917E-4 64-65 0.0019932936741717863 66-67 0.006533573709785301 68-69 2.2147707490797626E-4 70-71 0.0 72-73 0.0 74-75 3.3221561236196443E-4 76-77 0.05658739263898793 78-79 0.13476880008150358 80-81 0.0013288624494478577 82-83 7.75169762177917E-4 84-85 0.0 86-87 0.10187945445766908 88-89 0.35148411787895834 90-91 0.3881385737762284 92-93 4.429541498159525E-4 94-95 1.1073853745398813E-4 96-97 1.1073853745398813E-4 98-99 0.0 100-101 9.966468370858931E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 451514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.58551095103466 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2763608512397 58.3103193112688 2 8.672215500724437 11.722322313878882 3 2.087103923166028 4.2317395516525504 4 0.8626988169107008 2.332237613510513 5 0.4963899616198496 1.677438459352101 6 0.3272670977484825 1.3271108411276038 7 0.22555675078237422 1.0671057775057367 8 0.16696803774956973 0.9027696115037053 9 0.1377404671176795 0.8378333863904304 >10 0.6870828071069015 8.466888398342217 >50 0.03964600055590679 1.8105415505564757 >100 0.016710297643676444 2.040845259700844 >500 0.0016382644748702394 0.7675314312199571 >1k 0.002293570264818335 3.3586697107077583 >5k 3.276528949740479E-4 1.1466467832824403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5178 1.1468082938735011 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4829 1.0695127947306176 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3181 0.7045185752822725 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2408 0.5333167963784069 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1357 0.30054439065012384 RNA PCR Primer, Index 39 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1239 0.2744100958109826 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1133 0.25093352587073714 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1020 0.2259066164061358 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 963 0.21328242313638116 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 768 0.1700943935293258 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 679 0.1503829338625159 RNA PCR Primer, Index 39 (96% over 25bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 539 0.11937614337539922 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.11450364772742373 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.214770749079763E-4 0.0 10-11 0.0 0.0 0.0 2.214770749079763E-4 0.0 12-13 0.0 0.0 0.0 2.214770749079763E-4 0.0 14-15 1.1073853745398814E-4 0.0 0.0 2.214770749079763E-4 0.0 16-17 2.214770749079763E-4 0.0 0.0 2.214770749079763E-4 0.0 18-19 2.214770749079763E-4 0.0 0.0 5.536926872699408E-4 0.0 20-21 2.214770749079763E-4 0.0 0.0 0.001550339524355834 0.0 22-23 2.214770749079763E-4 0.0 0.0 0.004208064423251549 0.0 24-25 2.214770749079763E-4 0.0 0.0 0.011073853745398814 0.0 26-27 2.214770749079763E-4 0.0 0.0 0.021815491878435664 0.0 28-29 2.214770749079763E-4 0.0 0.0 0.05448336042736217 0.0 30-31 2.214770749079763E-4 0.0 0.0 0.13222181372006184 0.0 32-33 2.214770749079763E-4 0.0 0.0 0.25968187032960216 0.0 34-35 2.214770749079763E-4 0.0 0.0 0.45524612747334525 0.0 36-37 2.214770749079763E-4 0.0 0.0 0.7493676829511378 0.0 38-39 2.214770749079763E-4 0.0 0.0 1.131637114242305 0.0 40-41 2.214770749079763E-4 0.0 0.0 1.5386012393857111 0.0 42-43 2.214770749079763E-4 0.0 0.0 1.9197632853023383 0.0 44-45 2.214770749079763E-4 0.0 0.0 2.3562945999459597 0.0 46-47 2.214770749079763E-4 0.0 0.0 2.8584938672997957 0.0 48-49 2.214770749079763E-4 0.0 0.0 3.3729851123110244 0.0 50-51 2.214770749079763E-4 0.0 0.0 3.8958924861687567 0.0 52-53 2.214770749079763E-4 0.0 0.0 4.368635302559833 0.0 54-55 2.214770749079763E-4 0.0 0.0 4.908264195573116 0.0 56-57 2.214770749079763E-4 0.0 0.0 5.500715370951953 0.0 58-59 2.214770749079763E-4 0.0 0.0 6.116089423583765 0.0 60-61 2.214770749079763E-4 0.0 0.0 6.786611267867663 0.0 62-63 2.214770749079763E-4 0.0 0.0 7.429448477788064 0.0 64-65 2.214770749079763E-4 0.0 0.0 8.10960457483046 0.0 66-67 2.214770749079763E-4 0.0 0.0 8.849670220635463 0.0 68-69 2.214770749079763E-4 0.0 0.0 9.64012190098203 0.0 70-71 2.214770749079763E-4 0.0 0.0 10.501335506761695 0.0 72-73 2.214770749079763E-4 0.0 0.0 11.356569231518845 0.0 74-75 2.214770749079763E-4 0.0 0.0 12.233950663766795 0.0 76-77 2.214770749079763E-4 0.0 0.0 13.161053699331582 0.0 78-79 2.214770749079763E-4 0.0 0.0 14.150059577333149 0.0 80-81 2.214770749079763E-4 0.0 0.0 15.16243128673751 0.0 82-83 2.214770749079763E-4 0.0 0.0 16.191856730909784 0.0 84-85 2.214770749079763E-4 0.0 0.0 17.222943253143868 0.0 86-87 2.214770749079763E-4 0.0 0.0 18.271526464295683 0.0 88-89 2.214770749079763E-4 0.0 0.0 19.33417346970415 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTCA 25 0.005038598 56.944782 9 GGTATCA 3355 0.0 43.377506 1 GTATCAA 5105 0.0 32.23414 1 CGACCGT 35 0.008430394 27.116564 18-19 TATCAAC 6225 0.0 26.607672 2 ATCAACG 6315 0.0 26.07816 3 TCAACGC 6345 0.0 25.954857 4 CTAATAC 110 9.519525E-5 25.88686 3 CAACGCA 6500 0.0 25.335936 5 AACGCAG 6550 0.0 25.139744 6 CGCAGAA 140 1.7897524E-5 23.726994 9 ATGGGAT 660 0.0 23.010542 5 TAGAGTG 105 0.0022051441 22.59964 5 GAGTACT 4485 0.0 22.435839 12-13 GTCCTGC 150 2.9988954E-5 22.194351 1 CGCAGAG 7510 0.0 21.862955 8 ACGCAGA 7540 0.0 21.713032 7 TTAGAGC 175 4.0704217E-6 21.695654 3 GTATAGG 110 0.0028539356 21.617874 1 GTGGTAT 1830 0.0 21.310736 1 >>END_MODULE