FastQCFastQC Report
Thu 26 May 2016
SRR938831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165094
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22471.3610427998594739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18471.1187565871564078No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15600.944916229541958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.7535101215065356No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.7498758283159896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.284080584394345No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.2640919718463421No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.22290331568682087No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.17626321974148063No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1990.12053739081977541No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.1187202442245024No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.1084230801846221No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCGA459.755426E-442.0856139
GGTATCA13950.034.310611
CGATACC404.4602185E-429.71756294-95
GTATCAA19650.028.698931
GTTTACT700.00846680927.0796641
GTGTTGT700.00850448627.0550386
GAACTGT1057.0238835E-527.0550386
ATTCTTA700.00850448627.0550386
TATCAAC23750.023.7373892
ATCAACG24600.022.9171943
ACACTGA1252.2943418E-422.726236
TCAACGC25050.022.6946284
AACGCAG25050.022.6808726
CAACGCA25200.022.5595425
TAGGAGG1050.002222064622.5595424
TACATGG9250.022.5351542
GTACATG9450.022.064911
AGTACTT17800.021.27924312-13
GAGTACT17400.020.81605312-13
TACTGTG1150.003780280720.5853587
Produced by FastQC (version 0.11.5)