FastQCFastQC Report
Thu 26 May 2016
SRR938831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165094
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21581.3071341175330418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18461.1181508716246502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15620.9461276606054732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.7777387427768423No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.6838528353544041No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.3337492579984736No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT3960.2398633505760355No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.23501762632197415No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.2132118671786982No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.15506317612996232No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1980.11993167528801775TruSeq Adapter, Index 1 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.11750881316098707No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1830.11084594231165275No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1740.10539450252583377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG156.637448E-494.893065
CTACTAG250.00496995457.12621
GGTATCA11650.037.185151
GTATCAA18400.034.151531
CTGGACT851.6363483E-533.491674
GTGTGAG650.005767051629.2954861
CTGGCCC650.005861944529.1978664
TATCAAC21900.028.814562
ATCAACG22250.028.1480543
TCAACGC22300.028.0849424
CAACGCA22700.027.799075
AACGCAG22700.027.5900546
CCTCTAC700.00828122727.2029531
GGGTACC350.0084292427.11230542-43
TATAGAG700.00841724427.1123055
ACGCAGA25200.024.8529457
CGCAGAG25250.024.803738
TGGACTG1151.2850543E-424.7547115
GAGTACT15350.024.72783512-13
GCAGAGT25350.023.9572229