##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938831_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 165094 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.865216179873283 31.0 31.0 34.0 30.0 34.0 2 32.072722206742824 33.0 31.0 34.0 30.0 34.0 3 32.10794456491453 34.0 31.0 34.0 30.0 34.0 4 35.81967848619574 37.0 35.0 37.0 35.0 37.0 5 35.72904527117884 37.0 35.0 37.0 33.0 37.0 6 35.77160284444014 37.0 35.0 37.0 33.0 37.0 7 35.69936521012272 37.0 35.0 37.0 33.0 37.0 8 35.69108507880359 37.0 35.0 37.0 33.0 37.0 9 37.27370467733534 39.0 37.0 39.0 33.0 39.0 10-11 37.23395156698608 39.0 37.0 39.0 33.0 39.0 12-13 37.168282917610576 39.0 37.0 39.0 33.0 39.0 14-15 38.27934086035834 40.0 38.0 41.0 33.0 41.0 16-17 38.20646722473258 40.0 37.0 41.0 33.0 41.0 18-19 38.257102014609856 40.0 37.5 41.0 33.0 41.0 20-21 38.30706446024689 40.0 37.5 41.0 33.0 41.0 22-23 38.208042085115146 40.0 37.0 41.0 33.0 41.0 24-25 38.096620713048324 40.0 37.0 41.0 33.0 41.0 26-27 37.84864986007971 40.0 37.0 41.0 33.0 41.0 28-29 37.61072782778296 40.0 36.0 41.0 32.5 41.0 30-31 37.21560141495148 39.0 35.5 41.0 32.0 41.0 32-33 36.80518674209844 39.0 35.0 41.0 31.0 41.0 34-35 36.5576883472446 39.0 35.0 41.0 30.5 41.0 36-37 36.32681381515985 39.0 35.0 41.0 30.0 41.0 38-39 36.08937635528851 39.0 35.0 41.0 30.0 41.0 40-41 35.79455340593843 39.0 35.0 41.0 29.5 41.0 42-43 35.4636600966722 38.5 35.0 41.0 27.5 41.0 44-45 34.983460937405354 38.0 35.0 41.0 25.0 41.0 46-47 34.37150047851527 38.0 34.0 40.5 22.0 41.0 48-49 33.80365731038076 38.0 33.5 40.0 17.0 41.0 50-51 33.43434649351279 38.0 33.0 40.0 10.5 41.0 52-53 33.160775073594436 38.0 33.0 40.0 6.0 41.0 54-55 32.8730268816553 38.0 33.0 40.0 2.0 41.0 56-57 32.5586362920518 37.0 33.0 40.0 2.0 41.0 58-59 32.217742619356244 37.0 32.5 40.0 2.0 41.0 60-61 31.835617890413946 36.0 31.5 40.0 2.0 41.0 62-63 31.422153439858505 36.0 31.0 39.0 2.0 41.0 64-65 31.016687462899924 35.0 31.0 39.0 2.0 40.5 66-67 30.570256944528573 35.0 30.5 39.0 2.0 40.0 68-69 30.0778829030734 35.0 29.5 38.0 2.0 40.0 70-71 29.6154281803094 35.0 29.0 37.5 2.0 40.0 72-73 29.274046906610778 35.0 29.0 37.0 2.0 39.0 74-75 28.874289798539017 34.5 29.0 36.5 2.0 39.0 76-77 26.921677953166075 32.0 25.5 34.5 2.0 36.5 78-79 27.856009303790565 34.0 26.5 35.0 2.0 37.0 80-81 27.854401129053752 34.0 27.0 35.0 2.0 37.0 82-83 27.563824245581305 34.0 26.5 35.0 2.0 37.0 84-85 27.188210958605403 34.0 26.0 35.0 2.0 36.0 86-87 26.671989896664932 33.5 25.0 35.0 2.0 36.0 88-89 26.174421844524936 33.0 23.5 35.0 2.0 35.0 90-91 25.75332840684701 33.0 21.5 35.0 2.0 35.0 92-93 25.451294414091365 33.0 19.5 35.0 2.0 35.0 94-95 25.1048311870813 33.0 17.5 35.0 2.0 35.0 96-97 24.626600603292673 32.5 4.5 35.0 2.0 35.0 98-99 23.979457157740438 32.0 2.0 35.0 2.0 35.0 100-101 21.424421844524936 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18772667241626095 1101 2 0.078542489211749 1101 3 0.017504635602790586 1101 4 0.21805690329637883 1101 5 -0.050793353744147396 1101 6 0.16861839214818275 1101 7 0.21963896752536272 1101 8 0.18925124524432135 1101 9 0.35893159045604506 1101 10-11 0.3533497594416346 1101 12-13 0.20606607419755107 1101 14-15 0.20559495678195816 1101 16-17 0.1607078882637083 1101 18-19 0.2717276297222142 1101 20-21 0.1399509881976897 1101 22-23 0.18354537088651313 1101 24-25 0.5764927794326837 1101 26-27 0.41445752390598045 1101 28-29 0.39564990006194023 1101 30-31 0.5822038091400685 1101 32-33 0.7658182293315079 1101 34-35 1.0171665782361927 1101 36-37 0.9118414544960345 1101 38-39 1.0864346492910784 1101 40-41 0.964413221554338 1101 42-43 1.1196897202676794 1101 44-45 0.8838100331766299 1101 46-47 1.18763030407505 1101 48-49 1.243964536012328 1101 50-51 1.484886616110579 1101 52-53 2.0026454411311363 1101 54-55 1.439779528809538 1101 56-57 0.9900065783260104 1101 58-59 0.8676932417528818 1101 60-61 0.8624889771530668 1101 62-63 1.2917936886410963 1101 64-65 1.1804311341910712 1101 66-67 0.8543274537839913 1101 68-69 1.2904845609345905 1101 70-71 1.4994790003838467 1101 72-73 1.9036351578475497 1101 74-75 1.8359261766053443 1101 76-77 0.9532593237873108 1101 78-79 0.8134893559720346 1101 80-81 0.793679171936212 1101 82-83 0.8405967638253102 1101 84-85 0.5323970589128599 1101 86-87 0.6964242670422003 1101 88-89 0.7218154409319162 1101 90-91 0.5259388188591281 1101 92-93 0.34401737141726585 1101 94-95 0.687764765116043 1101 96-97 0.4531724408043445 1101 98-99 1.0612234580386186 1101 100-101 2.363053338789456 1102 1 0.08207139183069145 1102 2 0.07749682870285568 1102 3 0.05615050524377807 1102 4 0.040207478409662656 1102 5 0.33954300038620744 1102 6 0.19515202756122108 1102 7 0.16753021883243946 1102 8 0.23617526058067995 1102 9 0.3808904611426911 1102 10-11 0.3364666991418801 1102 12-13 0.43057809929339896 1102 14-15 0.13645065563172665 1102 16-17 0.427983071254296 1102 18-19 0.24188273603103738 1102 20-21 0.3541806849561411 1102 22-23 0.4968949700499792 1102 24-25 0.2760832290667068 1102 26-27 0.42905320184254236 1102 28-29 0.3627769478137708 1102 30-31 0.16239289941542268 1102 32-33 0.5282354478745575 1102 34-35 0.4707436085603476 1102 36-37 0.35454797244654657 1102 38-39 0.6150596057218891 1102 40-41 0.3937657834225874 1102 42-43 0.1946505079985954 1102 44-45 0.15422219769388334 1102 46-47 0.32173626433995395 1102 48-49 0.6837083491876541 1102 50-51 0.5143611717048628 1102 52-53 0.340045231999035 1102 54-55 0.9473169983111092 1102 56-57 0.5846824235682604 1102 58-59 0.22506950525933433 1102 60-61 0.56116447384181 1102 62-63 0.36226685002136705 1102 64-65 0.764127544089984 1102 66-67 0.41386474900880543 1102 68-69 0.3107224487003606 1102 70-71 0.2977318759502481 1102 72-73 0.21458845167815 1102 74-75 0.37032405042897665 1102 76-77 0.25630481001944716 1102 78-79 0.07183285544920537 1102 80-81 -0.22045902952771712 1102 82-83 0.09776389522998485 1102 84-85 0.6358738801049135 1102 86-87 0.604602203605463 1102 88-89 0.7550805158709188 1102 90-91 0.3195951451052075 1102 92-93 0.535482167513468 1102 94-95 0.10613789164096588 1102 96-97 0.8401975366565573 1102 98-99 0.9120643433645128 1102 100-101 0.7225209233136773 1103 1 0.10798979522171948 1103 2 0.04040746098826986 1103 3 -0.014363447423122011 1103 4 -0.043243598948166095 1103 5 0.24677542709055444 1103 6 0.356093391037021 1103 7 0.2856863474089124 1103 8 0.4760639924775347 1103 9 0.394666512008115 1103 10-11 0.1629635200532178 1103 12-13 0.3419610028610407 1103 14-15 0.47418467188040836 1103 16-17 -0.11614886092514354 1103 18-19 0.27221654034045883 1103 20-21 0.15252932501265803 1103 22-23 0.2522817591704367 1103 24-25 0.18940890903844831 1103 26-27 0.5759535903978232 1103 28-29 0.4828537757897493 1103 30-31 0.260878416900411 1103 32-33 0.6028998774047594 1103 34-35 0.25953290562852516 1103 36-37 0.29048050512051304 1103 38-39 0.9240052079825674 1103 40-41 0.5112375248567034 1103 42-43 0.28889724060191924 1103 44-45 0.2750496438259944 1103 46-47 -0.07255903767346439 1103 48-49 0.35012480980228133 1103 50-51 0.8811264489920418 1103 52-53 0.9786888208369007 1103 54-55 0.9881594568063434 1103 56-57 1.1559028403821046 1103 58-59 0.762710798088726 1103 60-61 1.450426218808257 1103 62-63 1.4668014667645757 1103 64-65 0.8922183247328697 1103 66-67 0.5717815628555023 1103 68-69 0.49080457515744413 1103 70-71 0.6225066905034069 1103 72-73 0.6249116590253792 1103 74-75 0.889906353502095 1103 76-77 0.9155020901324811 1103 78-79 -0.14648664296125347 1103 80-81 0.38628328767468645 1103 82-83 0.10114167693255993 1103 84-85 0.4689275713800818 1103 86-87 0.8602759937856099 1103 88-89 1.0801202544791835 1103 90-91 1.1617004082631013 1103 92-93 1.1043844988451532 1103 94-95 0.8408826814678818 1103 96-97 0.29776553383070237 1103 98-99 0.11051453764215324 1103 100-101 0.23366981063264802 1104 1 -0.24157294628733084 1104 2 0.016571788830447076 1104 3 -0.30832317896674866 1104 4 -0.21505567948507576 1104 5 -0.13178873805079405 1104 6 -0.5255417523430879 1104 7 -0.56755750046581 1104 8 -0.11466205999348489 1104 9 -0.10332006517309367 1104 10-11 -0.09469358793946014 1104 12-13 -0.3984091415359501 1104 14-15 -0.3861569998706571 1104 16-17 -0.31952490322736793 1104 18-19 0.06863712199594119 1104 20-21 -0.41834722572966854 1104 22-23 -0.5509519199021682 1104 24-25 -0.31606190649948473 1104 26-27 -0.6366963994333688 1104 28-29 -0.5131600537811281 1104 30-31 -0.4229938629450274 1104 32-33 -1.352825158186043 1104 34-35 -1.0573066306740984 1104 36-37 -1.1028284230877432 1104 38-39 -0.9928750035285105 1104 40-41 -1.1194519199266963 1104 42-43 -1.2620281203905606 1104 44-45 -1.6196015662614656 1104 46-47 -1.8464455538418676 1104 48-49 -2.3513394977022983 1104 50-51 -1.4258900244673853 1104 52-53 -1.6065257408877294 1104 54-55 -1.6478584402853826 1104 56-57 -1.9283558539437031 1104 58-59 -1.798993493145769 1104 60-61 -1.8479264352490965 1104 62-63 -2.4841645694363628 1104 64-65 -2.488623652082264 1104 66-67 -2.350549126589282 1104 68-69 -2.384871650183211 1104 70-71 -2.8630536104931323 1104 72-73 -2.592070240506853 1104 74-75 -2.6022636688692664 1104 76-77 -2.7050388901400453 1104 78-79 -2.7364805416799847 1104 80-81 -2.021994117247015 1104 82-83 -2.5093932020586927 1104 84-85 -2.745186725955694 1104 86-87 -2.709014223826756 1104 88-89 -2.234014380460824 1104 90-91 -2.214057167494474 1104 92-93 -1.6139396825662793 1104 94-95 -1.5255806091564814 1104 96-97 -1.8281038987501397 1104 98-99 -1.3776286390916255 1104 100-101 -1.0766616923163035 1105 1 0.11538516990645675 1105 2 0.12979317466329832 1105 3 -0.14653973947630305 1105 4 -0.0443550425423993 1105 5 -0.18463567879582143 1105 6 0.055234159643013925 1105 7 0.12713464390786555 1105 8 -0.1560054299992757 1105 9 0.02152069915811694 1105 10-11 -0.12051871822318816 1105 12-13 -0.3241570666469329 1105 14-15 -0.18650442314801552 1105 16-17 -0.07652162200752599 1105 18-19 -0.11819939605076257 1105 20-21 0.1901260419792763 1105 22-23 -0.11725186199829096 1105 24-25 -0.2736569653684242 1105 26-27 -0.45617140425869707 1105 28-29 -0.4131407524879407 1105 30-31 -0.5952965072221943 1105 32-33 -0.19071359671142574 1105 34-35 -0.18243691440311238 1105 36-37 -0.5324366068360575 1105 38-39 -0.024355412715927116 1105 40-41 -0.29629036810272424 1105 42-43 -0.830219589218963 1105 44-45 -1.5103562468250544 1105 46-47 -1.1193465382078145 1105 48-49 -1.15701407789917 1105 50-51 -1.2610955832398218 1105 52-53 -1.2922041813432372 1105 54-55 -0.8221684190539591 1105 56-57 -0.6097108047254096 1105 58-59 -0.339777125649146 1105 60-61 -0.9006189656486363 1105 62-63 -0.49947049493337303 1105 64-65 -0.7155489560776296 1105 66-67 -0.9909081306425485 1105 68-69 -1.2320826133177398 1105 70-71 -1.5482495186189809 1105 72-73 -1.5161775556969737 1105 74-75 -1.317762607298146 1105 76-77 -1.0443363230680482 1105 78-79 -1.0807191056637713 1105 80-81 -1.2024612085421325 1105 82-83 -1.5604451225373666 1105 84-85 -0.9620560562008187 1105 86-87 -0.7605293753419069 1105 88-89 -1.056288072798207 1105 90-91 -0.5230129675330772 1105 92-93 -1.3017412652723195 1105 94-95 -0.5570996209302699 1105 96-97 -0.9992677667292931 1105 98-99 -0.830707195354897 1105 100-101 -1.1093387816812914 1106 1 -0.33728478162180764 1106 2 -0.20618407166125863 1106 3 -0.133480411671151 1106 4 -0.15467832099451329 1106 5 -0.05300085926291587 1106 6 -0.17562751563639978 1106 7 -0.17887825518278788 1106 8 -0.11854999595689009 1106 9 -0.020301197248571157 1106 10-11 0.04498622909884631 1106 12-13 0.16522722210041252 1106 14-15 -0.002452025599644969 1106 16-17 -0.01849574370763918 1106 18-19 -0.2395389786329858 1106 20-21 -0.11160051332257837 1106 22-23 -0.20618348079410964 1106 24-25 -0.022865794435446674 1106 26-27 0.02608233126988324 1106 28-29 -0.22996965926997603 1106 30-31 -0.062387802338960796 1106 32-33 -0.3310412816851951 1106 34-35 -0.46994242255517804 1106 36-37 -0.6395351126246211 1106 38-39 -0.7921317216531563 1106 40-41 -0.3903495757300135 1106 42-43 0.06089926668777679 1106 44-45 -0.3468742935341851 1106 46-47 0.7509815473588262 1106 48-49 0.7579801135041038 1106 50-51 0.6285410790203159 1106 52-53 0.24967780399506267 1106 54-55 0.15151262889700945 1106 56-57 0.16060926898368422 1106 58-59 0.03165259033050205 1106 60-61 0.045441203401558994 1106 62-63 -0.030876976526130306 1106 64-65 0.3142351243614172 1106 66-67 -0.36958857770420295 1106 68-69 -0.035872221938500815 1106 70-71 0.2773123128916595 1106 72-73 0.06161758110549442 1106 74-75 0.14930865817475691 1106 76-77 -0.15049343559458705 1106 78-79 0.08799058467793586 1106 80-81 0.24530148023726284 1106 82-83 0.5243403599767547 1106 84-85 0.27345249645711434 1106 86-87 -0.15675579043624666 1106 88-89 0.24351563669183562 1106 90-91 0.1271092132945455 1106 92-93 0.43408776168758223 1106 94-95 -0.008828179196505914 1106 96-97 -0.23159160601143114 1106 98-99 -0.06379216110420316 1106 100-101 0.753698513515598 1107 1 -0.31632042103480273 1107 2 -0.1359534636948041 1107 3 -0.3675824382260089 1107 4 -0.10517913627520414 1107 5 0.15687568394696427 1107 6 -0.02049124729258267 1107 7 -0.05891552515716825 1107 8 -0.12274286807429036 1107 9 -0.3033200651730965 1107 10-11 -0.18201120859040998 1107 12-13 -0.18353820999834625 1107 14-15 -0.40927708965741516 1107 16-17 -0.4202544206235501 1107 18-19 -0.08877028541146359 1107 20-21 0.15965501893924028 1107 22-23 -0.2829945686114925 1107 24-25 -0.24625270335694438 1107 26-27 -0.4441599235635678 1107 28-29 -0.14733513795621178 1107 30-31 -0.4759680492525433 1107 32-33 -0.6152830706439616 1107 34-35 -0.2767791334799412 1107 36-37 -0.10232337258269553 1107 38-39 -0.4699793806328856 1107 40-41 -0.8326393497807985 1107 42-43 -1.3041717792008782 1107 44-45 -0.7789730589662867 1107 46-47 -0.47456003195634366 1107 48-49 -0.7483091946719895 1107 50-51 -0.855968587879282 1107 52-53 -0.8236974580594492 1107 54-55 -0.8929201686804404 1107 56-57 -0.8137093892972374 1107 58-59 -0.718522112674389 1107 60-61 -0.44192194591127887 1107 62-63 -0.4715944235328884 1107 64-65 -0.7149985117904585 1107 66-67 -0.7256333016734544 1107 68-69 -0.704344152989048 1107 70-71 -0.5734913209308417 1107 72-73 -0.3901061552094376 1107 74-75 -0.8213994713384061 1107 76-77 -0.4683946701624926 1107 78-79 -0.5510147728808796 1107 80-81 -0.1824879444075087 1107 82-83 0.16148221881672598 1107 84-85 -0.0632001939691591 1107 86-87 0.10366815552228914 1107 88-89 0.4355142390677962 1107 90-91 0.49503374159643343 1107 92-93 0.8664194644595362 1107 94-95 0.48109728085474046 1107 96-97 0.9383944177481744 1107 98-99 -0.021455799585453406 1107 100-101 -0.15836764068891895 1108 1 0.009780828143366449 1108 2 -0.2283269022220864 1108 3 -0.2740036523395304 1108 4 -0.12866514694069764 1108 5 0.19157507762071901 1108 6 -0.10004417299935398 1108 7 0.19260029046121474 1108 8 -0.13391972540499353 1108 9 0.00437224251921009 1108 10-11 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-3.7641877788765754 2316 100-101 -4.186045856539177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 17.0 6 42.0 7 93.0 8 165.0 9 311.0 10 501.0 11 726.0 12 1083.0 13 1417.0 14 1656.0 15 2042.0 16 2541.0 17 3317.0 18 3151.0 19 1942.0 20 1621.0 21 1596.0 22 1654.0 23 1752.0 24 1913.0 25 2159.0 26 2311.0 27 2594.0 28 3118.0 29 3613.0 30 4358.0 31 5240.0 32 6253.0 33 7652.0 34 9923.0 35 14794.0 36 18427.0 37 28900.0 38 25526.0 39 2683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50737194102447 21.79905760753914 15.989664082687337 19.70390636874905 2 15.979987158830728 23.72527166341599 38.5852908040268 21.709450373726483 3 18.644529783032695 28.216046615867324 32.89943910741759 20.239984493682385 4 12.657031751608175 19.977709668431316 36.77541279513489 30.589845784825613 5 11.47285788702194 39.32850376149345 34.677819908658094 14.52081844282651 6 27.282033265897006 37.95898094418937 18.456152252656064 16.302833537257563 7 23.372139508401276 37.07221340569615 22.61802367136298 16.937623414539598 8 24.76407380038039 34.37072213405696 21.534398585048518 19.330805480514133 9 25.24864622578652 17.43249300398561 22.965098671060126 34.353762099167746 10-11 22.936024325535755 28.626418888633143 27.739954207905797 20.697602577925302 12-13 24.69350794093062 27.35744484960083 28.779059202636077 19.169988006832472 14-15 22.124668370746363 28.050383417931602 29.0634426447963 20.761505566525738 16-17 18.60458777280409 31.021981816089305 30.176690431337867 20.196739979768733 18-19 18.13482016305862 28.489830036221793 34.65389414515367 18.72145565556592 20-21 19.55704023162562 27.68241123238882 34.54940821592547 18.21114032006009 22-23 19.28234823797352 28.159109355882105 34.14479024071135 18.413752165433024 24-25 18.862890232231337 28.365658352211465 34.71416284056355 18.057288574993642 26-27 18.673906986322944 28.52556725259549 34.67903133972161 18.12149442135995 28-29 18.170557379432324 28.633081759482476 35.02580348165288 18.170557379432324 30-31 18.46766084775946 28.94805383599646 34.43644226925267 18.14784304699141 32-33 18.149357335820802 29.015591117787444 34.2813790931227 18.553672453269048 34-35 18.45509420932916 29.039067871302272 34.14190914349924 18.36392877586933 36-37 18.871993699642577 28.720239898224996 33.86169503846853 18.5460713636639 38-39 18.379851887749695 28.701367539489027 33.990095561175806 18.928685011585465 40-41 18.397659366320678 28.896891573052425 34.105467839002685 18.59998122162421 42-43 18.320173725002046 29.089851806758194 33.48143571478328 19.10853875345648 44-45 18.25230251103176 29.56754284745126 33.02592804596239 19.154226595554587 46-47 18.686796795735724 29.344155549161833 32.191044414494904 19.77800324060754 48-49 18.587895380813354 29.48017493064557 31.828231189504162 20.103698499036913 50-51 18.58407079646018 29.838030685734708 31.698194351062735 19.879704166742382 52-53 18.631809756865785 29.98594739966322 31.45541327970732 19.926829563763672 54-55 18.162985935285352 29.830581365767383 31.215852786897162 20.790579912050106 56-57 18.559729608586622 29.995941705937224 30.703720304796057 20.740608380680097 58-59 18.33445895810556 29.674699488471685 30.926717284447903 21.064124268974854 60-61 18.431788239926103 29.59098687099656 30.90570437784878 21.071520511228552 62-63 18.26474614462064 29.71700970356282 30.905726434637238 21.112517717179305 64-65 18.253843265049003 29.680969629423238 30.885132106557478 21.18005499897028 66-67 18.17284249738783 29.879310083740933 30.296954737495646 21.65089268137559 68-69 18.195557036206974 29.612187107227765 30.312703484410253 21.879552372155004 70-71 18.234185994390973 29.51705423069701 30.181233380378092 22.067526394533925 72-73 18.159048784328927 29.188825759870134 30.033798926672077 22.61832652912886 74-75 18.172374526027596 29.2551516105976 29.995033132639588 22.57744073073522 76-77 18.106335959548804 29.092485426474557 30.175003561912096 22.626175052064546 78-79 17.955826213415744 28.943525419620673 30.206932072956434 22.89371629400715 80-81 17.96067694767829 28.845385053363536 29.97898167104801 23.214956327910162 82-83 18.02669994063988 28.736659115413037 29.824827068215683 23.4118138757314 84-85 17.944625486086714 28.38019552497365 29.821495632791017 23.85368335614862 86-87 17.982598463642677 28.329203829672377 29.63901971547366 24.049177991211284 88-89 18.12279347872321 28.05206326829476 29.677521282866152 24.14762197011588 90-91 17.860287999611305 27.74926663953891 29.7000358329335 24.690409527916284 92-93 17.973396973845205 27.674234072710092 29.671278180915117 24.68109077252959 94-95 17.933419748749195 27.494942275309825 29.548923643500068 25.022714332440916 96-97 18.003985608198967 27.043078488618605 29.652500999430625 25.3004349037518 98-99 17.911722483691012 27.12138007062686 29.581205744640897 25.385691701041228 100-101 17.888564826172153 26.81664410342144 30.167816029292847 25.126975041113564 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1596.0 1 1059.5 2 507.5 3 489.5 4 438.0 5 417.0 6 624.5 7 998.0 8 1253.5 9 1286.5 10 1016.0 11 710.0 12 642.0 13 622.5 14 603.5 15 609.5 16 631.5 17 653.0 18 673.0 19 712.5 20 746.5 21 848.5 22 927.0 23 984.5 24 1171.5 25 1377.5 26 1629.0 27 1946.5 28 2191.5 29 2417.0 30 2651.5 31 2872.0 32 3230.0 33 3535.0 34 3782.0 35 4111.0 36 4395.0 37 4582.0 38 4742.5 39 4708.0 40 4537.0 41 4681.0 42 4825.0 43 4909.5 44 5109.5 45 5229.5 46 5343.5 47 5489.5 48 5789.5 49 5847.5 50 5578.5 51 5220.0 52 4646.5 53 4175.5 54 3860.5 55 3564.0 56 3228.0 57 2793.0 58 2497.0 59 2242.5 60 1891.5 61 1550.0 62 1184.5 63 833.0 64 598.0 65 469.0 66 348.5 67 267.5 68 211.0 69 146.5 70 94.0 71 75.0 72 67.0 73 58.0 74 41.5 75 29.5 76 20.0 77 13.5 78 14.0 79 7.0 80 3.5 81 3.5 82 2.0 83 0.5 84 0.5 85 0.0 86 1.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3749379141579948 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0018171465952729958 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0057542975516978205 36-37 0.014537172762183966 38-39 0.009994306274001477 40-41 0.006360013083455486 42-43 0.0033314354246671593 44-45 0.0015142888293941633 46-47 9.085732976364979E-4 48-49 0.0 50-51 6.057155317576653E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.0285776587883265E-4 60-61 9.085732976364979E-4 62-63 0.0 64-65 0.0 66-67 9.085732976364979E-4 68-69 9.085732976364979E-4 70-71 0.0018171465952729958 72-73 0.0 74-75 0.0 76-77 0.0935830496565593 78-79 0.03876579403249058 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1353774213478382 88-89 0.3180006541727743 90-91 0.2671205495051304 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.057155317576653E-4 100-101 0.0015142888293941633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 165094.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.10958796321471 #Duplication Level Percentage of deduplicated Percentage of total 1 86.35281883038795 59.67807728830239 2 8.686221589373153 12.00602389997517 3 2.0698781047345722 4.291452688568582 4 0.9251830480925967 2.5575607697692164 5 0.5503767425179616 1.9018154949976318 6 0.33893809148048015 1.4054323108352613 7 0.2073432849970874 1.0030586295161676 8 0.1505222926400441 0.8322026898905498 9 0.11762655279986683 0.7316210337778903 >10 0.555237056541852 6.579076280862722 >50 0.02691454290718223 1.2434798562271094 >100 0.012958853992346997 1.8938094874062739 >500 9.968349224882306E-4 0.3795885876903808 >1k 0.0049841746124411525 5.496800982180669 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2158 1.3071341175330418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1846 1.1181508716246502 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1562 0.9461276606054732 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1284 0.7777387427768423 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1129 0.6838528353544041 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 551 0.3337492579984736 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 396 0.2398633505760355 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 388 0.23501762632197415 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 0.2132118671786982 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.15506317612996232 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 198 0.11993167528801775 TruSeq Adapter, Index 1 (95% over 21bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.11750881316098707 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 183 0.11084594231165275 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 174 0.10539450252583377 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.057155317576653E-4 0.0 22-23 0.0 0.0 0.0 0.003937150956424824 0.0 24-25 0.0 0.0 0.0 0.010902879571637976 0.0 26-27 0.0 0.0 0.0 0.022108616909154784 0.0 28-29 0.0 0.0 0.0 0.06087441094164536 0.0 30-31 0.0 0.0 0.0 0.14143457666541487 0.0 32-33 0.0 0.0 0.0 0.29801204162477135 0.0 34-35 0.0 0.0 0.0 0.5660411644275383 0.0 36-37 0.0 0.0 0.0 0.9464305183713522 0.0 38-39 0.0 0.0 0.0 1.366191381879414 0.0 40-41 0.0 0.0 0.0 1.8104837244236616 0.0 42-43 0.0 0.0 0.0 2.27264467515476 0.0 44-45 0.0 0.0 0.0 2.8553430167056346 0.0 46-47 0.0 0.0 0.0 3.469235708142028 0.0 48-49 0.0 0.0 0.0 4.073739808836178 0.0 50-51 0.0 0.0 0.0 4.625546658267412 0.0 52-53 0.0 0.0 0.0 5.1622106194047035 0.0 54-55 0.0 0.0 0.0 5.77792045743637 0.0 56-57 0.0 0.0 0.0 6.487213345124596 0.0 58-59 6.057155317576653E-4 0.0 0.0 7.22043199631725 0.0 60-61 6.057155317576653E-4 0.0 0.0 7.963039238252147 0.0 62-63 6.057155317576653E-4 0.0 0.0 8.691715022956618 0.0 64-65 6.057155317576653E-4 0.0 0.0 9.452190873078369 0.0 66-67 6.057155317576653E-4 0.0 0.0 10.319575514555344 0.0 68-69 6.057155317576653E-4 0.0 0.0 11.249954571335119 0.0 70-71 6.057155317576653E-4 0.0 0.0 12.208499400341623 0.0 72-73 6.057155317576653E-4 0.0 0.0 13.175221389026857 0.0 74-75 6.057155317576653E-4 0.0 0.0 14.23825214726156 0.0 76-77 6.057155317576653E-4 0.0 0.0 15.277962857523594 0.0 78-79 6.057155317576653E-4 0.0 0.0 16.400353737870546 0.0 80-81 6.057155317576653E-4 0.0 0.0 17.59876191745309 0.0 82-83 6.057155317576653E-4 0.0 0.0 18.7832386398052 0.0 84-85 6.057155317576653E-4 0.0 0.0 19.95620676705392 0.0 86-87 6.057155317576653E-4 0.0 0.0 21.140986347171914 0.0 88-89 6.057155317576653E-4 0.0 0.0 22.38179461397749 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCCCG 15 6.637448E-4 94.89306 5 CTACTAG 25 0.004969954 57.1262 1 GGTATCA 1165 0.0 37.18515 1 GTATCAA 1840 0.0 34.15153 1 CTGGACT 85 1.6363483E-5 33.49167 4 GTGTGAG 65 0.0057670516 29.295486 1 CTGGCCC 65 0.0058619445 29.197866 4 TATCAAC 2190 0.0 28.81456 2 ATCAACG 2225 0.0 28.148054 3 TCAACGC 2230 0.0 28.084942 4 CAACGCA 2270 0.0 27.79907 5 AACGCAG 2270 0.0 27.590054 6 CCTCTAC 70 0.008281227 27.202953 1 GGGTACC 35 0.00842924 27.112305 42-43 TATAGAG 70 0.008417244 27.112305 5 ACGCAGA 2520 0.0 24.852945 7 CGCAGAG 2525 0.0 24.80373 8 TGGACTG 115 1.2850543E-4 24.754711 5 GAGTACT 1535 0.0 24.727835 12-13 GCAGAGT 2535 0.0 23.957222 9 >>END_MODULE