FastQCFastQC Report
Thu 26 May 2016
SRR938830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164770
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21571.30909752989015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18301.1106390726467197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16360.9928991928142259No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.7677368452995084No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.7173635977423074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.29677732596953327No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.27796322146021724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.21848637494689568No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.15840262183649936No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.12745038538568915No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.1244158524003156No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1860.11288462705589611No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1710.10378102809977545No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG200.00207654571.201163
TCTATAC200.00207654571.201163
TGTGCGT250.005027266756.9609224
TATGAGT459.6334267E-442.1932754
TGGACAG550.002581890234.5217745
GGTATCA12350.030.7856141
GTATCAA17550.028.9755331
CCTCTAG700.00834928527.1573071
CAGACCT1109.472308E-525.891334
GTACATG10700.025.3172111
CATGGGC1701.11802365E-725.1298224
TACATGG10200.024.6644522
TATCAAC20800.024.4183442
ATCAACG21050.024.1283423
TCAACGC21400.023.955534
ATGGGCA1000.001654230823.7337195
TGGGAAC1401.7768747E-523.7337176
CAACGCA21650.023.6789075
AACGCAG21650.023.6789076
ACATGGG10250.022.6917513
Produced by FastQC (version 0.11.5)