##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938830_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 164770 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.583443588031802 33.0 31.0 34.0 30.0 34.0 2 31.612787522000364 33.0 31.0 34.0 30.0 34.0 3 31.581040237907388 33.0 31.0 34.0 28.0 34.0 4 35.18098561631365 37.0 35.0 37.0 33.0 37.0 5 35.23355586575226 37.0 35.0 37.0 33.0 37.0 6 35.31497238574983 37.0 35.0 37.0 33.0 37.0 7 35.24989986041148 37.0 35.0 37.0 33.0 37.0 8 35.228682405777754 37.0 35.0 37.0 32.0 37.0 9 36.77623960672452 39.0 37.0 39.0 33.0 39.0 10-11 36.68812283789525 39.0 37.0 39.0 32.0 39.0 12-13 36.58073071554288 39.0 37.0 39.0 32.0 39.0 14-15 37.670628755234574 40.0 37.0 41.0 32.0 41.0 16-17 37.570179644352734 40.0 37.0 41.0 32.0 41.0 18-19 37.56314256235966 40.0 37.0 41.0 32.0 41.0 20-21 37.50019724464405 40.0 37.0 41.0 32.0 41.0 22-23 37.407073496388904 40.0 37.0 41.0 32.0 41.0 24-25 37.29752078655095 40.0 36.5 41.0 32.0 41.0 26-27 37.087603932754746 40.0 36.0 41.0 31.0 41.0 28-29 36.85774716271166 39.5 36.0 41.0 31.0 41.0 30-31 36.505392365115014 39.0 35.0 41.0 30.0 41.0 32-33 36.149247435819625 39.0 35.0 41.0 30.0 41.0 34-35 35.752376039327544 38.0 35.0 41.0 29.5 41.0 36-37 35.42606360381137 38.0 35.0 40.0 27.5 41.0 38-39 35.15010013958852 38.0 35.0 40.0 26.5 41.0 40-41 34.86793408994356 38.0 35.0 40.0 24.5 41.0 42-43 34.61819202524731 38.0 35.0 40.0 23.5 41.0 44-45 34.23702433695455 38.0 34.5 40.0 21.0 41.0 46-47 33.60983492140559 38.0 33.5 40.0 12.0 41.0 48-49 33.08174728409298 38.0 33.0 40.0 4.0 41.0 50-51 31.829174000121384 36.5 31.5 39.0 2.0 40.5 52-53 31.84492929538144 37.0 31.5 39.5 2.0 40.5 54-55 32.259337257995995 37.5 33.0 40.0 2.0 41.0 56-57 32.12373308247861 37.0 32.5 40.0 2.0 41.0 58-59 31.884317533531586 37.0 32.0 40.0 2.0 41.0 60-61 31.55764702312314 36.5 31.5 40.0 2.0 41.0 62-63 31.1436729987255 36.0 31.0 40.0 2.0 41.0 64-65 30.724042604843113 35.0 30.5 39.0 2.0 41.0 66-67 30.312353583783455 35.0 30.0 39.0 2.0 41.0 68-69 29.83636584329672 35.0 29.0 38.5 2.0 40.0 70-71 29.367931055410573 35.0 29.0 38.0 2.0 40.0 72-73 28.89843721551253 35.0 28.0 37.0 2.0 39.5 74-75 28.388332220671238 35.0 27.0 36.5 2.0 39.0 76-77 27.859540571706013 34.0 26.0 36.0 2.0 39.0 78-79 27.37624871032348 34.0 25.5 35.5 2.0 38.0 80-81 26.831140377495903 34.0 24.5 35.0 2.0 37.0 82-83 26.37435516174061 34.0 22.5 35.0 2.0 37.0 84-85 25.97729865873642 34.0 20.0 35.0 2.0 36.0 86-87 25.452278934272016 33.0 15.0 35.0 2.0 36.0 88-89 24.905489470170544 33.0 4.5 35.0 2.0 35.5 90-91 24.38595011227772 33.0 2.0 35.0 2.0 35.0 92-93 23.835491897796928 32.0 2.0 35.0 2.0 35.0 94-95 23.26598288523396 32.0 2.0 35.0 2.0 35.0 96-97 22.625040966195304 31.5 2.0 35.0 2.0 35.0 98-99 21.860878800752566 31.0 2.0 34.5 2.0 35.0 100-101 19.782460399344544 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.009809966412543503 1101 2 0.10498727879818759 1101 3 0.2326101679087742 1101 4 -0.2365239866225508 1101 5 -0.4689609396726624 1101 6 -0.34832896841754035 1101 7 -0.0036143623242566036 1101 8 -0.3951755479957697 1101 9 -0.38541418964125995 1101 10-11 -0.04834171967813461 1101 12-13 -0.4788992915774273 1101 14-15 -0.32827785002309184 1101 16-17 -0.2318702891677944 1101 18-19 -0.3422813974905736 1101 20-21 0.23428797205481544 1101 22-23 0.1442283434771383 1101 24-25 0.04857810533219009 1101 26-27 0.29828247608421776 1101 28-29 -0.057391899620746756 1101 30-31 -0.490530327509056 1101 32-33 -0.3716640855351869 1101 34-35 -0.4422208349255854 1101 36-37 -0.5541992370689641 1101 38-39 -0.605459909575714 1101 40-41 0.13058279949526508 1101 42-43 0.5295062279581586 1101 44-45 0.8365862836817897 1101 46-47 0.881424294048351 1101 48-49 0.15931760983011145 1101 50-51 0.16114300583844354 1101 52-53 0.7324588719829492 1101 54-55 0.0721981603695312 1101 56-57 -0.01446729306247363 1101 58-59 -0.6391336778330867 1101 60-61 -0.7361167928919095 1101 62-63 -0.7475618198957221 1101 64-65 -0.398312100935005 1101 66-67 -1.063910123639424 1101 68-69 -1.115801053333314 1101 70-71 -0.9717116829756769 1101 72-73 -1.5163687092016538 1101 74-75 -1.223016271754446 1101 76-77 -0.8399863837425698 1101 78-79 -1.1068081284689661 1101 80-81 -0.9026535779801037 1101 82-83 -0.8369055750192622 1101 84-85 -0.987523088613802 1101 86-87 -1.5947899201310456 1101 88-89 -1.651234249349578 1101 90-91 -1.924961813909281 1101 92-93 -1.859167137854314 1101 94-95 -1.4648093694478241 1101 96-97 -1.7220738844974761 1101 98-99 -2.1582356755798173 1101 100-101 -2.246543100086292 1102 1 -0.09687618630941586 1102 2 0.12256532450922464 1102 3 0.0759909985018794 1102 4 0.3159462768451533 1102 5 0.3296019637495746 1102 6 -0.04430590991878347 1102 7 -0.22482168583336914 1102 8 -0.07844595760888495 1102 9 0.014212327632314725 1102 10-11 0.14590989401081345 1102 12-13 0.3437979032425318 1102 14-15 0.052370061315350824 1102 16-17 -0.10701744948119796 1102 18-19 -0.15044524273881166 1102 20-21 0.08842672900687631 1102 22-23 0.08231952197751724 1102 24-25 0.23176732304825975 1102 26-27 0.4654768900816961 1102 28-29 0.009571117470152046 1102 30-31 0.5588749418450618 1102 32-33 0.27312136487685734 1102 34-35 0.28300548748743637 1102 36-37 0.2630783986229943 1102 38-39 -0.2183369444476284 1102 40-41 -0.2410528112707766 1102 42-43 -0.08775516935439498 1102 44-45 -0.02587464271775275 1102 46-47 0.5398499831645793 1102 48-49 0.018855627935906227 1102 50-51 0.5008296051158361 1102 52-53 0.48638653000267595 1102 54-55 0.08167731434996739 1102 56-57 -0.08222193114959708 1102 58-59 -0.35624081054124446 1102 60-61 0.03618053183508607 1102 62-63 0.10533876062634562 1102 64-65 -0.02223123380554526 1102 66-67 0.03216018854342195 1102 68-69 -0.11975915831443729 1102 70-71 -0.5408262853383619 1102 72-73 -0.5874319170935074 1102 74-75 -0.39680752738713565 1102 76-77 0.11219158889576164 1102 78-79 -0.44385599109227414 1102 80-81 -0.48481977779336205 1102 82-83 -0.45319266956722615 1102 84-85 -0.412745351431969 1102 86-87 -0.7508610442328596 1102 88-89 -0.5160700387377979 1102 90-91 -0.608130733651091 1102 92-93 -0.582871112197676 1102 94-95 -0.6403665488413175 1102 96-97 -0.44076425919808315 1102 98-99 -0.4289903542628828 1102 100-101 -0.4178174069104692 1103 1 -0.09349885711687023 1103 2 -0.23500455126717412 1103 3 0.04939938359504836 1103 4 0.25069006566502594 1103 5 -0.0041325083001169105 1103 6 -0.15486491612995934 1103 7 0.007088252054828104 1103 8 -0.16474254145981604 1103 9 0.3945694704894578 1103 10-11 0.14222200581205868 1103 12-13 -0.47211824582579 1103 14-15 -0.7646136343368184 1103 16-17 -0.4192068904749888 1103 18-19 -0.14095378311148465 1103 20-21 -0.2917945442850538 1103 22-23 -0.09856168920260444 1103 24-25 0.02276111186813523 1103 26-27 0.03807005778356398 1103 28-29 -0.04101118066649434 1103 30-31 -0.2274992817574244 1103 32-33 -0.24223189599270256 1103 34-35 -0.6064160963634961 1103 36-37 -0.1096118187683075 1103 38-39 -0.10523523637310461 1103 40-41 0.035791132828606464 1103 42-43 0.02899560704312165 1103 44-45 0.267060139890944 1103 46-47 0.7041162875124058 1103 48-49 0.04502022420919616 1103 50-51 -0.2589141837040394 1103 52-53 -0.2853720103699935 1103 54-55 -0.2370338657742579 1103 56-57 -0.1842793845657411 1103 58-59 -0.3240397235847219 1103 60-61 -0.3405081327611903 1103 62-63 -0.5266876368891857 1103 64-65 -0.47655964995461275 1103 66-67 0.11950490904031597 1103 68-69 -0.4767079160784107 1103 70-71 -0.11889305552469764 1103 72-73 -0.5777657680251842 1103 74-75 -0.4611902913622927 1103 76-77 -0.7149046843961671 1103 78-79 -1.058646363763085 1103 80-81 -1.1812483492219314 1103 82-83 -0.7396833527970408 1103 84-85 -0.9326211278294849 1103 86-87 -0.5482212926800649 1103 88-89 -0.9466795107874866 1103 90-91 -0.6143613237132044 1103 92-93 -0.6362096215144426 1103 94-95 -1.1103976047419408 1103 96-97 -0.7513426753471499 1103 98-99 -0.6696735840765484 1103 100-101 -0.07156352492289031 1104 1 0.2834828502002047 1104 2 0.4069161804401418 1104 3 0.19650304213163494 1104 4 8.42504689416046E-4 1104 5 -0.12654104488547802 1104 6 0.1390375228944265 1104 7 -0.11699711379883126 1104 8 -0.002699858532992039 1104 9 -0.12951589536420016 1104 10-11 0.17499639605595974 1104 12-13 0.6495280956376277 1104 14-15 0.38256624371195613 1104 16-17 0.7121955485494098 1104 18-19 0.4498236559129012 1104 20-21 0.21956216303202325 1104 22-23 0.6330694083583737 1104 24-25 0.17885867284374513 1104 26-27 0.5121554236372248 1104 28-29 0.2216412583578915 1104 30-31 0.7689946206816032 1104 32-33 0.45830164059265854 1104 34-35 0.7855808548560219 1104 36-37 1.045952205621937 1104 38-39 0.6305117148464134 1104 40-41 0.5431844255115266 1104 42-43 0.13760841192117113 1104 44-45 -0.17287888449930477 1104 46-47 0.2694364094636299 1104 48-49 -0.17357733676641374 1104 50-51 -0.11089589102111219 1104 52-53 0.4647042091422051 1104 54-55 0.30412467081111316 1104 56-57 0.31709256665376984 1104 58-59 0.14097552031771698 1104 60-61 0.1752693062631998 1104 62-63 -0.12104739298674616 1104 64-65 0.4812909598014876 1104 66-67 0.5295658846500721 1104 68-69 0.43411525465329603 1104 70-71 0.4193691395972543 1104 72-73 0.695252524657743 1104 74-75 0.18797129400356383 1104 76-77 -0.01277053805470274 1104 78-79 -0.09568904668991252 1104 80-81 0.3736296995585562 1104 82-83 0.3700727447639345 1104 84-85 0.6484764331461257 1104 86-87 0.8359250487833485 1104 88-89 0.8753479282369021 1104 90-91 1.2881539201892345 1104 92-93 0.515314768729457 1104 94-95 0.6164316635507419 1104 96-97 0.49263598318943735 1104 98-99 -0.053819925539961844 1104 100-101 -0.11721901272776947 1105 1 0.4779026580346475 1105 2 0.667078782066163 1105 3 0.40943726238292655 1105 4 0.5266370353620005 1105 5 0.45439021897261256 1105 6 0.08149872023367521 1105 7 0.03928522175179694 1105 8 0.15931048884321086 1105 9 0.25403916745915467 1105 10-11 0.5420326118726635 1105 12-13 0.13886660265905704 1105 14-15 0.6546098505116618 1105 16-17 0.5523840186159177 1105 18-19 0.32486061082790485 1105 20-21 0.8147395466240397 1105 22-23 0.2172527047367936 1105 24-25 0.13033686944388734 1105 26-27 0.07784278505629061 1105 28-29 0.48152669812137816 1105 30-31 0.39087193036379375 1105 32-33 -0.3097508353866445 1105 34-35 -0.0034804903028842205 1105 36-37 0.25108893880744887 1105 38-39 0.16114733938447756 1105 40-41 0.11846158737405688 1105 42-43 -0.10424302932051432 1105 44-45 -0.17119743586663816 1105 46-47 -0.5609405306694057 1105 48-49 -0.24020704851807295 1105 50-51 -0.12198236552222141 1105 52-53 0.10714692902394773 1105 54-55 0.3360721948318073 1105 56-57 -0.22272635426271137 1105 58-59 0.3946155794455848 1105 60-61 0.5033365642085066 1105 62-63 0.2689563025047548 1105 64-65 -0.1993816196515823 1105 66-67 0.057004909040315965 1105 68-69 -0.24432155244204523 1105 70-71 -0.2016582070398485 1105 72-73 -0.33401576802518207 1105 74-75 -0.15617135196835363 1105 76-77 0.032443800452320204 1105 78-79 -0.4652751516418725 1105 80-81 -0.16230895528253697 1105 82-83 -0.4237742618879494 1105 84-85 -0.28413627934464003 1105 86-87 -0.03988795934673206 1105 88-89 0.014778822545846282 1105 90-91 -0.0960848085616881 1105 92-93 0.37553280272797807 1105 94-95 0.6797539104095769 1105 96-97 -0.3270055541350274 1105 98-99 -0.24391600831897264 1105 100-101 -0.3063173128016814 1106 1 -0.1613452426590385 1106 2 0.06868520776897213 1106 3 -6.759176097723696E-4 1106 4 -0.33944547650364854 1106 5 -0.2909547974567417 1106 6 -0.1741420245637002 1106 7 0.2418774086813329 1106 8 0.06635685613053965 1106 9 -0.05979799939005659 1106 10-11 0.09139369255904484 1106 12-13 0.2608636818850485 1106 14-15 0.12518305241017202 1106 16-17 0.4216063625370623 1106 18-19 0.03875254218971236 1106 20-21 0.4117822629438592 1106 22-23 0.41186752766486734 1106 24-25 0.54926713596452 1106 26-27 -0.1825624723369188 1106 28-29 0.06960628921302003 1106 30-31 -0.02486373958874566 1106 32-33 -0.5384291851493259 1106 34-35 -0.2529145903393939 1106 36-37 -0.0703422404550551 1106 38-39 0.14924518531364583 1106 40-41 0.6265667352382422 1106 42-43 0.9128434986093836 1106 44-45 1.203957730252391 1106 46-47 0.9801328537774694 1106 48-49 0.9078214290284734 1106 50-51 0.40923340665741037 1106 52-53 0.3864276884251865 1106 54-55 0.512213122177549 1106 56-57 1.1837176033860644 1106 58-59 0.8439949149694996 1106 60-61 1.3786560238653216 1106 62-63 1.5428153751590088 1106 64-65 1.0769418560694852 1106 66-67 1.0780892463897125 1106 68-69 1.139632445367372 1106 70-71 0.8564834504994003 1106 72-73 1.0346589307699965 1106 74-75 0.8050371182762603 1106 76-77 0.8873543517484137 1106 78-79 1.1334470097308937 1106 80-81 0.7720649037901168 1106 82-83 0.9244732737089834 1106 84-85 0.8908728480741281 1106 86-87 1.1159353338259592 1106 88-89 1.6193972964414307 1106 90-91 1.4188841582145066 1106 92-93 1.1621337519795318 1106 94-95 1.1941204675472186 1106 96-97 0.8391317222432129 1106 98-99 0.4779167773692379 1106 100-101 0.654453041342169 1107 1 -0.20328659296592733 1107 2 -0.3643677588143426 1107 3 -0.3234386667194187 1107 4 -0.01035553181925053 1107 5 -0.0904925711931952 1107 6 0.23633005242349725 1107 7 0.14757567343847455 1107 8 0.1378124899867217 1107 9 -0.5473330452338132 1107 10-11 0.11961980455419763 1107 12-13 -0.1788006357628973 1107 14-15 -0.02269146452550075 1107 16-17 -0.33280751940580444 1107 18-19 -0.2948649466334956 1107 20-21 -0.44409407258693534 1107 22-23 -0.398070337001343 1107 24-25 -0.1196917183205457 1107 26-27 -0.4890525837258721 1107 28-29 -0.17399074041492923 1107 30-31 0.08629788805389893 1107 32-33 -0.11626884567824192 1107 34-35 -0.4315536749798454 1107 36-37 -0.35805914581233367 1107 38-39 -0.4152391671907054 1107 40-41 -0.8411153137122795 1107 42-43 -0.33391712880592905 1107 44-45 0.38128969963937465 1107 46-47 0.2635305956885148 1107 48-49 0.09352651351736796 1107 50-51 -1.0124912277291962 1107 52-53 -0.9850771990492362 1107 54-55 -1.396231978981806 1107 56-57 -1.3190671204148003 1107 58-59 -0.7191065474840919 1107 60-61 -0.9892306170379186 1107 62-63 -1.1320728570149683 1107 64-65 -0.9617601216527234 1107 66-67 -1.2448818834125142 1107 68-69 -0.4544987965815537 1107 70-71 -0.477816011499538 1107 72-73 -0.7849591642515996 1107 74-75 -0.8565715806704688 1107 76-77 -1.1781908479181809 1107 78-79 -0.9578995084171673 1107 80-81 -1.3738584121150126 1107 82-83 -1.3945575666335195 1107 84-85 -1.8961431404081033 1107 86-87 -2.204667833560567 1107 88-89 -1.4518878441208187 1107 90-91 -1.6080916696251535 1107 92-93 -2.1178920114515485 1107 94-95 -1.8850831393331369 1107 96-97 -2.0343811973597283 1107 98-99 -2.2215603765293785 1107 100-101 -2.1454825500801284 1108 1 -0.0014872677128963119 1108 2 0.13506995204408412 1108 3 -0.41107247070958763 1108 4 -0.017894371421064648 1108 5 -0.1404321771742829 1108 6 -0.19592451877896622 1108 7 -0.05888856913722407 1108 8 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-0.482294590605612 2316 50-51 -0.37138111492096826 2316 52-53 -0.8497073278303091 2316 54-55 -0.3901423313827266 2316 56-57 -0.40117753271389134 2316 58-59 -0.434340649510645 2316 60-61 -0.053421492549549754 2316 62-63 -0.4979508644024122 2316 64-65 -0.29000211027207 2316 66-67 -0.46415911212370276 2316 68-69 -1.1296510377715308 2316 70-71 -0.47606898145062004 2316 72-73 -0.22131735532677155 2316 74-75 -0.381279577076576 2316 76-77 -0.7322988642903461 2316 78-79 -0.5906238769905947 2316 80-81 0.10777281479923317 2316 82-83 0.49642775831406993 2316 84-85 0.3652624700541125 2316 86-87 0.5516464321876597 2316 88-89 0.10366771143473485 2316 90-91 0.0448483323714548 2316 92-93 0.05711048430566379 2316 94-95 0.5169833476390124 2316 96-97 0.5507241235946481 2316 98-99 -0.2212608856638525 2316 100-101 0.29002708354271434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1072.0 3 187.0 4 163.0 5 173.0 6 246.0 7 296.0 8 406.0 9 475.0 10 629.0 11 836.0 12 1201.0 13 1647.0 14 1819.0 15 2367.0 16 2625.0 17 3317.0 18 2881.0 19 1871.0 20 1673.0 21 1752.0 22 1918.0 23 1998.0 24 2069.0 25 2319.0 26 2649.0 27 3025.0 28 3363.0 29 3919.0 30 4525.0 31 5277.0 32 6237.0 33 7655.0 34 9997.0 35 14910.0 36 17653.0 37 24905.0 38 22886.0 39 3827.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45643097970958 20.90327056732841 15.783804179802086 19.856494273159925 2 15.937368936110724 23.016794412803385 39.18392404524705 21.86191260583884 3 18.604750148974205 28.035729487163895 32.47212054141483 20.88739982244707 4 12.379875635952516 19.229015846386694 37.79913322027511 30.59197529738568 5 12.062661596958176 38.69079847908745 34.43650190114069 14.810038022813687 6 27.130774235523745 37.440942727366696 19.153101988945572 16.27518104816398 7 23.45551330798479 36.714220532319395 23.18722433460076 16.643041825095057 8 24.250555200632775 33.6831857868638 22.309026193301086 19.757232819202336 9 25.712757616827826 17.41796677950856 23.3260603997885 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18.51467238375988 28.79478152826672 34.284287332659304 18.406258755314095 36-37 18.720811564180288 28.568033997958903 34.00740278137423 18.703751656486574 38-39 18.601003286417868 28.77884753031332 33.91619786733106 18.703951315937754 40-41 18.417687215682456 28.78983490551675 34.02249572191536 18.76998215688543 42-43 18.39759990253411 28.83101851851852 33.455470272904485 19.315911306042885 44-45 18.23492860275842 29.154685900872362 33.302670695452996 19.307714800916223 46-47 18.71064823981309 29.1669839683937 32.577089089186664 19.545278702606545 48-49 18.71022188911942 29.334576375496223 32.02905306388566 19.926148671498687 50-51 18.73394892292132 29.622634036965838 31.789586563858535 19.85383047625431 52-53 18.664318876960383 29.780473538622765 31.61778146058516 19.93742612383169 54-55 18.385226750012947 29.700641851652147 31.57380075486872 20.34033064346618 56-57 18.736789527864577 29.75896789284213 31.02717321784259 20.4770693614507 58-59 18.47720836758929 29.226881032855275 31.455193203617434 20.840717395938004 60-61 18.49287962641631 29.143606884752842 31.669376000779316 20.69413748805153 62-63 18.62395409119015 28.707322199309782 31.868731019820107 20.79999268967996 64-65 18.5343842640692 28.820262204578068 31.756734111883294 20.888619419469435 66-67 18.323029625318785 28.93031362069964 31.40625047608311 21.34040627789847 68-69 18.34331787405462 28.924594879545605 31.344652531888574 21.387434714511205 70-71 18.246667174525786 28.7919555115411 31.204692618267693 21.756684695665424 72-73 18.33333841170789 28.496384705154654 31.10524728129218 22.06502960184528 74-75 18.20145638463179 28.660004265561685 30.94329849791292 22.195240851893605 76-77 18.401372045487086 28.286557670690804 31.160420265087474 22.151650018734635 78-79 18.14660055794462 28.171634972650967 31.388039519046867 22.293724950357547 80-81 18.09409808203657 28.13287158447476 31.155864606462657 22.617165727026016 82-83 18.168885802102466 27.75244379053311 31.158671991373243 22.919998415991177 84-85 18.157974899476056 27.173449494334108 31.362251736322648 23.30632386986719 86-87 18.31384900303992 27.216431412923626 31.073597767881623 23.396121816154835 88-89 18.50525026731126 26.90811499105916 31.135440751335032 23.451193990294545 90-91 18.43453886231376 26.6877913530971 31.285457481490507 23.592212303098627 92-93 18.430826510994454 26.55060311811609 31.22137893001758 23.797191440871877 94-95 18.372564254917194 26.081021375139024 31.302466596584246 24.24394777335954 96-97 18.70943561804121 25.544057870328054 31.190315130019318 24.556191381611423 98-99 18.815625095219232 25.472744446814342 31.184374904780764 24.527255553185654 100-101 19.12966658035429 24.835422600642772 31.82489756751405 24.21001325148889 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1812.0 1 1187.5 2 584.0 3 562.0 4 469.5 5 480.5 6 668.5 7 1012.0 8 1292.5 9 1273.5 10 978.0 11 755.0 12 698.0 13 650.5 14 668.0 15 655.0 16 640.5 17 657.0 18 700.0 19 760.0 20 809.0 21 906.0 22 1024.5 23 1151.0 24 1281.0 25 1443.5 26 1726.5 27 2033.0 28 2230.0 29 2450.5 30 2746.0 31 3000.0 32 3188.5 33 3367.5 34 3624.5 35 3927.0 36 4302.0 37 4627.0 38 4669.5 39 4548.0 40 4472.0 41 4567.0 42 4725.0 43 4826.5 44 5022.5 45 5159.5 46 5202.5 47 5305.5 48 5726.0 49 5773.0 50 5189.0 51 4819.5 52 4539.5 53 4229.0 54 3912.5 55 3535.5 56 3190.5 57 2810.5 58 2482.5 59 2197.5 60 1797.0 61 1470.5 62 1233.5 63 944.0 64 689.0 65 517.5 66 377.0 67 304.0 68 242.5 69 179.0 70 146.0 71 111.5 72 79.0 73 71.5 74 63.0 75 55.5 76 42.0 77 28.0 78 21.5 79 14.0 80 10.0 81 6.0 82 5.0 83 3.5 84 3.5 85 4.5 86 2.5 87 2.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2767494082660678 2 0.15233355586575226 3 0.18935485828730958 4 0.15354736905990168 5 0.23972810584451054 6 0.18874795169023487 7 0.23972810584451054 8 0.2518662377860047 9 0.14140923711840747 10-11 0.20574133640832676 12-13 0.21241730897614855 14-15 0.16598895429993324 16-17 0.28372883413242705 18-19 0.22637616070886693 20-21 0.19360320446683255 22-23 0.24336954542695877 24-25 0.23365903987376344 26-27 0.35079201310918245 28-29 0.31589488377738667 30-31 0.2885840869090247 32-33 0.1844996055107119 34-35 0.35443345269163073 36-37 0.3899374886205013 38-39 0.36990957091703586 40-41 0.3404746009589124 42-43 0.3702130242155732 44-45 0.37506827699217093 46-47 0.3796200764702312 48-49 0.39843418097954725 50-51 0.39175820841172543 52-53 0.3908478485161133 54-55 0.38568914244097835 56-57 0.36475086484190083 58-59 0.34290222734721126 60-61 0.31741215027007347 62-63 0.37537173029070825 64-65 0.40146871396492084 66-67 0.4069308733385932 68-69 0.41451720580202706 70-71 0.41421375250348974 72-73 0.4099654063239667 74-75 0.4035928870546823 76-77 0.38568914244097835 78-79 0.3617163318565273 80-81 0.3711233841111853 82-83 0.38204770285853007 84-85 0.38235115615706744 86-87 0.37688899678339505 88-89 0.38568914244097835 90-91 0.4035928870546823 92-93 0.40328943375614495 94-95 0.41117921951811615 96-97 0.4120895794137282 98-99 0.40966195302542935 100-101 0.3869029556351278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 164770.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.16800749158301 #Duplication Level Percentage of deduplicated Percentage of total 1 86.89992016645563 60.97594249257825 2 8.386073255931608 11.768681020943463 3 2.025926690065971 4.264657174978412 4 0.8473605807310267 2.378304143072273 5 0.5113651684503907 1.7940737485380818 6 0.29298969579063133 1.233510190151619 7 0.19232718685282946 0.9446650841547097 8 0.14548958916772908 0.816697166613484 9 0.10263971296679912 0.6481821733549463 >10 0.556047890441246 6.48811164905503 >50 0.023916037887677717 1.168405480248819 >100 0.01096151736518562 1.876021486240324 >500 0.0 0.0 >1k 0.004982507893266191 5.6427481900705985 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2157 1.30909752989015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1830 1.1106390726467197 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1636 0.9928991928142259 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1265 0.7677368452995084 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1182 0.7173635977423074 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 489 0.29677732596953327 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 458 0.27796322146021724 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 360 0.21848637494689568 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 261 0.15840262183649936 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 210 0.12745038538568915 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.1244158524003156 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 186 0.11288462705589611 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 171 0.10378102809977545 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.069065970747103E-4 0.0 18-19 0.0 0.0 0.0 6.069065970747103E-4 0.0 20-21 0.0 0.0 0.0 0.0012138131941494205 6.069065970747103E-4 22-23 0.0 0.0 0.0 0.004248346179522971 6.069065970747103E-4 24-25 0.0 0.0 0.0 0.014869211628330398 6.069065970747103E-4 26-27 0.0 0.0 0.0 0.02670389027128725 6.069065970747103E-4 28-29 0.0 0.0 0.0 0.05765612672209747 6.069065970747103E-4 30-31 0.0 0.0 0.0 0.13048491837106269 6.069065970747103E-4 32-33 0.0 0.0 0.0 0.2761425016689931 6.069065970747103E-4 34-35 6.069065970747103E-4 0.0 0.0 0.525581113066699 6.069065970747103E-4 36-37 6.069065970747103E-4 0.0 0.0 0.8572555683680282 6.069065970747103E-4 38-39 6.069065970747103E-4 0.0 0.0 1.2590277356314863 6.069065970747103E-4 40-41 6.069065970747103E-4 0.0 0.0 1.6853796200764704 6.069065970747103E-4 42-43 9.103598956120653E-4 0.0 0.0 2.098986465982885 6.069065970747103E-4 44-45 0.0012138131941494205 0.0 0.0 2.620015779571524 6.069065970747103E-4 46-47 0.0012138131941494205 0.0 0.0 3.185956181343691 6.069065970747103E-4 48-49 0.0012138131941494205 0.0 0.0 3.7424895308612003 6.069065970747103E-4 50-51 0.0012138131941494205 0.0 0.0 4.302967773259695 6.069065970747103E-4 52-53 0.0012138131941494205 0.0 0.0 4.841900831462038 6.069065970747103E-4 54-55 0.0012138131941494205 0.0 0.0 5.458214480791407 6.069065970747103E-4 56-57 0.0012138131941494205 0.0 0.0 6.168902105965891 6.069065970747103E-4 58-59 0.0012138131941494205 0.0 0.0 6.909935060994113 6.069065970747103E-4 60-61 0.0012138131941494205 0.0 0.0 7.6719062936214115 6.069065970747103E-4 62-63 0.0012138131941494205 0.0 0.0 8.38107665230321 6.069065970747103E-4 64-65 0.0012138131941494205 0.0 0.0 9.159130909752989 6.069065970747103E-4 66-67 0.0012138131941494205 0.0 0.0 10.00576561267221 6.069065970747103E-4 68-69 0.0012138131941494205 0.0 0.0 10.912787522000365 6.069065970747103E-4 70-71 0.0012138131941494205 0.0 0.0 11.888086423499423 6.069065970747103E-4 72-73 0.0012138131941494205 0.0 0.0 12.839715967712568 6.069065970747103E-4 74-75 0.0012138131941494205 0.0 0.0 13.805911270255507 6.069065970747103E-4 76-77 0.0012138131941494205 0.0 0.0 14.798810463069733 6.069065970747103E-4 78-79 0.0012138131941494205 0.0 0.0 15.940401772167263 6.069065970747103E-4 80-81 0.0012138131941494205 0.0 0.0 17.107483158341932 6.069065970747103E-4 82-83 0.0012138131941494205 0.0 0.0 18.284275050069795 6.069065970747103E-4 84-85 0.0012138131941494205 0.0 0.0 19.461066941797657 6.069065970747103E-4 86-87 0.0012138131941494205 0.0 0.0 20.672149056260242 6.069065970747103E-4 88-89 0.0012138131941494205 6.069065970747103E-4 0.0 21.93208715178734 6.069065970747103E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTGCG 20 0.002076545 71.20116 3 TCTATAC 20 0.002076545 71.20116 3 TGTGCGT 25 0.0050272667 56.960922 4 TATGAGT 45 9.6334267E-4 42.193275 4 TGGACAG 55 0.0025818902 34.521774 5 GGTATCA 1235 0.0 30.785614 1 GTATCAA 1755 0.0 28.975533 1 CCTCTAG 70 0.008349285 27.157307 1 CAGACCT 110 9.472308E-5 25.89133 4 GTACATG 1070 0.0 25.317211 1 CATGGGC 170 1.11802365E-7 25.129822 4 TACATGG 1020 0.0 24.664452 2 TATCAAC 2080 0.0 24.418344 2 ATCAACG 2105 0.0 24.128342 3 TCAACGC 2140 0.0 23.95553 4 ATGGGCA 100 0.0016542308 23.733719 5 TGGGAAC 140 1.7768747E-5 23.733717 6 CAACGCA 2165 0.0 23.678907 5 AACGCAG 2165 0.0 23.678907 6 ACATGGG 1025 0.0 22.691751 3 >>END_MODULE