FastQCFastQC Report
Thu 26 May 2016
SRR938830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164770
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20441.2405170844207076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18701.1349153365297082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15740.9552709837955938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.8259998786186806No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.7088669053832615No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.33501244158524No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT3820.2318383200825393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.22880378709716573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.21727256175274626No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.17478909995751654No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.1292711051769133No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT2010.12198822601201674TruSeq Adapter, Index 1 (95% over 21bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1860.11288462705589611No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1760.10681556108514899No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC500.001619588237.962733
GGTATCA14050.036.948961
ACCAGTC300.00396590131.6356162-63
GAGGGCG600.00396019631.635617
TATCCTC600.00396019631.635615
GTATCAA18650.027.7341692
GCCCCCT350.00842325827.11623450-51
ATGGGAT2052.6619091E-823.1480065
TATCAAC22400.023.091173
ATCAACG23100.022.391444
GAGTACT15350.022.10370812-13
AACGCAG23450.022.0572387
TCAACGC23500.022.0103075
CAACGCA23550.021.9635776
GTACTTT16550.020.0709314-15
ACGCAGA25850.020.009378
CGCAGAG26000.019.8939329
CATGGGA6500.019.7114184
ATGGGAG2201.3430781E-619.412765
GTACATG10350.019.3268451
Produced by FastQC (version 0.11.5)