##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938830_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 164770 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.905741336408326 31.0 31.0 34.0 30.0 34.0 2 32.098822601201675 33.0 31.0 34.0 30.0 34.0 3 32.13998907568125 34.0 31.0 34.0 30.0 34.0 4 35.8237543242095 37.0 35.0 37.0 35.0 37.0 5 35.74167020695515 37.0 35.0 37.0 33.0 37.0 6 35.787515931298174 37.0 35.0 37.0 33.0 37.0 7 35.692917400012135 37.0 35.0 37.0 33.0 37.0 8 35.71396492079869 37.0 35.0 37.0 33.0 37.0 9 37.27423074588821 39.0 37.0 39.0 33.0 39.0 10-11 37.228937306548524 39.0 37.0 39.0 33.0 39.0 12-13 37.161874127571764 39.0 37.0 39.0 33.0 39.0 14-15 38.285230927960185 40.0 38.0 41.0 33.0 41.0 16-17 38.223238453601994 40.0 37.5 41.0 33.0 41.0 18-19 38.33759179462281 40.0 37.5 41.0 33.5 41.0 20-21 38.358129513867816 40.0 38.0 41.0 33.5 41.0 22-23 38.22235236997026 40.0 37.0 41.0 33.0 41.0 24-25 38.09590944953572 40.0 37.0 41.0 33.0 41.0 26-27 37.82186381015961 40.0 36.5 41.0 33.0 41.0 28-29 37.574549371851674 40.0 36.0 41.0 32.5 41.0 30-31 37.1974479577593 39.0 35.5 41.0 32.0 41.0 32-33 36.81197426716028 39.0 35.0 41.0 31.0 41.0 34-35 36.59442556290587 39.0 35.0 41.0 31.0 41.0 36-37 36.33174424955999 39.0 35.0 41.0 30.0 41.0 38-39 36.08044546944225 39.0 35.0 41.0 30.0 41.0 40-41 35.758669660739216 39.0 35.0 41.0 29.5 41.0 42-43 35.377180311949985 38.0 35.0 41.0 27.5 41.0 44-45 34.917096558839596 38.0 35.0 41.0 25.0 41.0 46-47 34.30895187230685 38.0 34.0 40.0 21.5 41.0 48-49 33.73733385931905 38.0 33.5 40.0 16.5 41.0 50-51 33.37119014383686 38.0 33.0 40.0 9.0 41.0 52-53 33.091940280390844 38.0 33.0 40.0 6.0 41.0 54-55 32.76795533167446 37.5 33.0 40.0 2.0 41.0 56-57 32.4549584268981 37.0 33.0 40.0 2.0 41.0 58-59 32.081847423681495 36.5 32.0 40.0 2.0 41.0 60-61 31.6882988408084 36.0 31.0 40.0 2.0 41.0 62-63 31.291418340717364 35.5 31.0 39.0 2.0 41.0 64-65 30.837488620501304 35.0 30.5 39.0 2.0 40.5 66-67 30.38553741579171 35.0 30.0 38.5 2.0 40.0 68-69 30.060035200582632 35.0 29.5 38.0 2.0 40.0 70-71 29.642792984159737 35.0 29.0 37.5 2.0 40.0 72-73 29.15294349699581 35.0 29.0 37.0 2.0 39.0 74-75 28.664644656187413 34.0 28.0 36.0 2.0 39.0 76-77 26.804973599563027 32.0 25.0 34.5 2.0 36.5 78-79 27.646613461188323 34.0 26.0 35.0 2.0 37.0 80-81 27.657337500758633 34.0 26.0 35.0 2.0 37.0 82-83 27.334441949383987 34.0 26.0 35.0 2.0 36.5 84-85 26.946910845420888 34.0 25.5 35.0 2.0 36.0 86-87 26.380430296777327 33.0 24.5 35.0 2.0 36.0 88-89 25.824324816410755 33.0 21.5 35.0 2.0 35.0 90-91 25.32211264186442 33.0 19.0 35.0 2.0 35.0 92-93 25.02735631486314 33.0 17.5 35.0 2.0 35.0 94-95 24.698370455786854 32.0 11.0 35.0 2.0 35.0 96-97 24.131829216483585 32.0 2.0 34.5 2.0 35.0 98-99 23.397490441221095 32.0 2.0 34.0 2.0 35.0 100-101 20.401450506767006 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02783302718005487 1101 2 0.010297299703523777 1101 3 -0.2762837288596991 1101 4 0.16890039019319403 1101 5 0.13724680780359222 1101 6 0.05946626070808492 1101 7 -0.1662778133312841 1101 8 -0.03195597327692212 1101 9 0.03249159482179209 1101 10-11 0.03959128505432119 1101 12-13 0.02488872747227333 1101 14-15 0.14647961314499724 1101 16-17 0.1641689199926546 1101 18-19 -0.22661787153502644 1101 20-21 -0.17642789528382963 1101 22-23 -0.15255768011964932 1101 24-25 -0.33120732838344225 1101 26-27 -0.6736579686709234 1101 28-29 -0.23780093492584342 1101 30-31 -0.2620926618147763 1101 32-33 -0.07585256964208043 1101 34-35 -0.0920256241771753 1101 36-37 0.11632140886086262 1101 38-39 -0.531298659864639 1101 40-41 -0.013921791502497172 1101 42-43 -0.44773726735976993 1101 44-45 -0.544823914788978 1101 46-47 -0.8328019804484939 1101 48-49 -1.2989210370499364 1101 50-51 -1.205575159935428 1101 52-53 -1.6445941811534226 1101 54-55 -2.070231214137742 1101 56-57 -1.7141795598721856 1101 58-59 -1.3404486805079614 1101 60-61 -1.5318819239199328 1101 62-63 -1.8947750467763171 1101 64-65 -2.089850933631201 1101 66-67 -1.4534051317933994 1101 68-69 -1.2048764867171542 1101 70-71 -1.4371982393050509 1101 72-73 -1.8465963402245222 1101 74-75 -1.9769506126061138 1101 76-77 -1.9742749599639815 1101 78-79 -2.4759523445547487 1101 80-81 -2.5530142969445357 1101 82-83 -2.3995297584517097 1101 84-85 -1.9162574135307153 1101 86-87 -2.5272027324391537 1101 88-89 -1.9694657673694245 1101 90-91 -1.7480291918382456 1101 92-93 -2.7900248206400278 1101 94-95 -2.4137398498624663 1101 96-97 -2.408102273222479 1101 98-99 -3.127114062436398 1101 100-101 -3.490427649141459 1102 1 0.26645601260610974 1102 2 0.33953044223589757 1102 3 -0.14653907737591965 1102 4 0.12542212932363128 1102 5 -0.4842365681554881 1102 6 -0.10795143100628479 1102 7 -0.11183864636014818 1102 8 0.2252193606247559 1102 9 0.21992308915865522 1102 10-11 0.23010807147903733 1102 12-13 0.26653611106338815 1102 14-15 0.2943909515934209 1102 16-17 0.2177555720447799 1102 18-19 0.17617512972344684 1102 20-21 0.35813955488463733 1102 22-23 0.08742526958197061 1102 24-25 -0.004856498196289749 1102 26-27 -0.09285173313323014 1102 28-29 0.11244061096788016 1102 30-31 0.024271159075901494 1102 32-33 -0.38876614086766637 1102 34-35 -0.1134196890495005 1102 36-37 -0.19096962349410518 1102 38-39 -0.30962448294749123 1102 40-41 0.19965349368404617 1102 42-43 -0.22902659468490327 1102 44-45 -0.4196057115656586 1102 46-47 -0.29188126433595585 1102 48-49 -0.7750303213433654 1102 50-51 -0.6994248737339888 1102 52-53 -0.30917015647108315 1102 54-55 -0.29700018259469374 1102 56-57 -0.794803113641521 1102 58-59 -0.601683609262075 1102 60-61 -0.503322674133063 1102 62-63 -0.6762064599212856 1102 64-65 -0.7363211159070104 1102 66-67 -0.8783432229718962 1102 68-69 -0.7682873583121719 1102 70-71 -0.5590063828523206 1102 72-73 -0.37962114434907335 1102 74-75 -0.5065653570546154 1102 76-77 -0.52150834607205 1102 78-79 -1.1621659604358818 1102 80-81 -0.663232297188113 1102 82-83 -0.4944430861016116 1102 84-85 -0.8659996292575869 1102 86-87 -0.9985825887294943 1102 88-89 -1.3929627023753106 1102 90-91 -1.7892949735132362 1102 92-93 -1.4114878986248414 1102 94-95 -0.7963972606528742 1102 96-97 -0.2925865016964764 1102 98-99 -0.6956400235454794 1102 100-101 -0.48519077178182 1103 1 0.26137060887939967 1103 2 0.2111142931675758 1103 3 0.2348273822514102 1103 4 0.13765039019319403 1103 5 -0.13885613337287595 1103 6 -0.10356478504355238 1103 7 0.07931042196283045 1103 8 0.006197621494315797 1103 9 0.40455041835120653 1103 10-11 0.029991611851706068 1103 12-13 0.1442923222435084 1103 14-15 0.03882029941950549 1103 16-17 -0.05114970745832892 1103 18-19 0.3077162787917729 1103 20-21 -0.027632960643309445 1103 22-23 -0.26977581737455125 1103 24-25 -0.04777187086709489 1103 26-27 0.002567521525151051 1103 28-29 0.04518517618527085 1103 30-31 0.025979233610065933 1103 32-33 0.21113680944289115 1103 34-35 0.2930438202672647 1103 36-37 0.05083938271707211 1103 38-39 -0.16073084940712334 1103 40-41 0.17803082287658611 1103 42-43 0.003815020221928478 1103 44-45 0.16581742508029862 1103 46-47 -0.03114756868378521 1103 48-49 -0.09312038345517237 1103 50-51 0.1681993498684946 1103 52-53 0.21518931557860554 1103 54-55 -0.015206704333820653 1103 56-57 0.11952142051996617 1103 58-59 0.4896983783155733 1103 60-61 0.3959979780408567 1103 62-63 0.07891776368119707 1103 64-65 0.1780616480681445 1103 66-67 0.469715783239284 1103 68-69 -0.2435202775668266 1103 70-71 0.23016287180606199 1103 72-73 0.06690447676893996 1103 74-75 0.23955265536774206 1103 76-77 -0.021721855388815214 1103 78-79 -0.0891263641625919 1103 80-81 -0.0899015518464985 1103 82-83 0.005964522594037902 1103 84-85 -0.11106950503398494 1103 86-87 -0.7387223402822904 1103 88-89 -0.32984158436288524 1103 90-91 0.2964969519526015 1103 92-93 0.3311557349155265 1103 94-95 0.8509047579806683 1103 96-97 0.807277628738305 1103 98-99 1.275050970243342 1103 100-101 1.818089507721286 1104 1 0.019144999123302142 1104 2 0.05684600048464716 1104 3 0.26165665054409715 1104 4 0.09572965848587955 1104 5 0.10245484223687384 1104 6 0.2511608247125494 1104 7 0.11528603171893081 1104 8 -0.15203408582275557 1104 9 0.2893065159121804 1104 10-11 0.06581478258340923 1104 12-13 -0.13787231190282512 1104 14-15 0.04850017746828428 1104 16-17 0.09930760961484708 1104 18-19 0.30032298610884567 1104 20-21 0.10918106374693792 1104 22-23 0.01094064604008338 1104 24-25 -0.19937248062319668 1104 26-27 -0.08859101506021716 1104 28-29 -0.2333971408879023 1104 30-31 -0.12402076638993975 1104 32-33 -0.617140629581499 1104 34-35 -0.8762397163180964 1104 36-37 -0.5633374465512233 1104 38-39 -0.4252887762363926 1104 40-41 -0.10596307956243578 1104 42-43 -0.2855142480707471 1104 44-45 -0.30986855052945117 1104 46-47 -0.7897603735618333 1104 48-49 -0.2586691639429688 1104 50-51 0.19411398401484092 1104 52-53 -0.2676612941774934 1104 54-55 -0.1674932896996708 1104 56-57 -0.16599687216295678 1104 58-59 0.05768618319361707 1104 60-61 0.105372978040851 1104 62-63 -0.09974077290416616 1104 64-65 0.15146103831204627 1104 66-67 0.2767279783612331 1104 68-69 0.3360528931648794 1104 70-71 0.009278725464600512 1104 72-73 0.04213313530552654 1104 74-75 0.31577216756286575 1104 76-77 0.9303360714404505 1104 78-79 0.46857180656911623 1104 80-81 0.23494600912911423 1104 82-83 0.11716879088672627 1104 84-85 -0.11213657820471568 1104 86-87 -0.006100389062776657 1104 88-89 0.2698535375883324 1104 90-91 0.6882652446355273 1104 92-93 0.5246770763789428 1104 94-95 0.37308463602944997 1104 96-97 0.8450062872748916 1104 98-99 1.3383131653652924 1104 100-101 1.3100864589407983 1105 1 0.2437569725157651 1105 2 0.3042961113493945 1105 3 0.107554654978685 1105 4 -0.27958445829165157 1105 5 0.018530230263486658 1105 6 -0.17951175474051695 1105 7 -0.06538654773413555 1105 8 0.1596067124034093 1105 9 -0.138631399830615 1105 10-11 -0.3179250548149568 1105 12-13 0.14978474648594187 1105 14-15 0.031149844874050814 1105 16-17 0.42138817132955353 1105 18-19 0.11747006667055615 1105 20-21 0.4211359787506339 1105 22-23 0.09717494020120654 1105 24-25 0.019178886708658638 1105 26-27 -0.10235672089908832 1105 28-29 0.02482532770042667 1105 30-31 -0.11265713002629951 1105 32-33 -0.6429351602540763 1105 34-35 -0.6112175433691007 1105 36-37 -0.4288954657677735 1105 38-39 -0.2113937281949987 1105 40-41 -0.18617372257795495 1105 42-43 -0.495806191899284 1105 44-45 0.20663181901969807 1105 46-47 -0.7789695383807498 1105 48-49 -1.1260749289097163 1105 50-51 -1.3071794380102943 1105 52-53 -1.764261441997153 1105 54-55 -1.405168825545939 1105 56-57 -1.201880094631548 1105 58-59 -0.7561349550177638 1105 60-61 -0.394627021959149 1105 62-63 -1.0263852666218334 1105 64-65 -0.9133209276894299 1105 66-67 -1.0041478531243548 1105 68-69 -0.8067778533244017 1105 70-71 -0.8260871281939366 1105 72-73 -0.7903871898977286 1105 74-75 -0.8005988597837721 1105 76-77 -0.24492261296457585 1105 78-79 -0.33941045507168255 1105 80-81 -0.7476667033616486 1105 82-83 -0.7293574471029274 1105 84-85 -0.5214861717006514 1105 86-87 -0.40652537058531735 1105 88-89 -0.3802203722416735 1105 90-91 -0.2735788056231563 1105 92-93 0.017045886430679502 1105 94-95 0.13527975798066905 1105 96-97 0.02612232570800188 1105 98-99 0.4271343035766755 1105 100-101 1.2142069319637088 1106 1 0.06807241610831838 1106 2 -0.05966883936254419 1106 3 0.14808039429960473 1106 4 0.2411895468196974 1106 5 0.12236374614519008 1106 6 0.3166912390528367 1106 7 0.21394897617970088 1106 8 0.3048421998075739 1106 9 0.2578636713632534 1106 10-11 0.0860910094420646 1106 12-13 0.21108449091821768 1106 14-15 0.37545433556408625 1106 16-17 0.37513041302360506 1106 18-19 0.286217784815868 1106 20-21 0.2594528827301872 1106 22-23 0.5106835199748474 1106 24-25 0.3284705990124195 1106 26-27 0.48344101550105734 1106 28-29 0.6353583689563607 1106 30-31 0.7690515227666879 1106 32-33 1.2924244600453036 1106 34-35 1.2469971335202743 1106 36-37 0.893685768259239 1106 38-39 1.3673941505928795 1106 40-41 1.5086784132380373 1106 42-43 1.555471646727959 1106 44-45 1.2922104973694601 1106 46-47 1.6413298409547679 1106 48-49 1.7398615442556746 1106 50-51 1.7728680245672948 1106 52-53 1.6035552794340262 1106 54-55 1.6371276330155737 1106 56-57 1.0156810590741898 1106 58-59 1.2771983783155676 1106 60-61 1.6595898455107267 1106 62-63 1.4617490889824047 1106 64-65 1.8509908649356142 1106 66-67 1.796673614564579 1106 68-69 1.8924736983367865 1106 70-71 2.0052381730108806 1106 72-73 2.085918030985809 1106 74-75 1.870576751753287 1106 76-77 1.985544710876244 1106 78-79 1.537040804512106 1106 80-81 2.0496315806836236 1106 82-83 1.8078094021121132 1106 84-85 2.1283130250864986 1106 86-87 2.568355972970725 1106 88-89 1.9925981746732582 1106 90-91 1.5713463495429636 1106 92-93 1.6631210963613121 1106 94-95 2.2467255411132 1106 96-97 2.3104779299431257 1106 98-99 2.370306994339728 1106 100-101 2.4598440257935756 1107 1 0.06754199252720028 1107 2 -0.09501778230412583 1107 3 -0.34756255485550724 1107 4 -0.1895901758445433 1107 5 -0.25454009563703295 1107 6 -0.13685881020078483 1107 7 -0.041601527722704645 1107 8 0.010835986274194909 1107 9 -0.106613103661374 1107 10-11 0.03176047977623142 1107 12-13 0.02365552979068042 1107 14-15 -0.43694621001445455 1107 16-17 -0.18821731752122162 1107 18-19 0.0038051152697633484 1107 20-21 -0.1824089040395407 1107 22-23 -0.015117798506629754 1107 24-25 -0.2799063048293604 1107 26-27 -0.39137901935534813 1107 28-29 -0.2587652955128448 1107 30-31 -0.3810962380880554 1107 32-33 0.09217061447433395 1107 34-35 -0.024939670298770977 1107 36-37 0.242230892151035 1107 38-39 -0.2678259751932828 1107 40-41 -0.25895817083409867 1107 42-43 0.2584533850018076 1107 44-45 0.19256663891678016 1107 46-47 0.23601045018413913 1107 48-49 -0.16992855955579955 1107 50-51 -0.6356528513893736 1107 52-53 -0.2695787661823985 1107 54-55 0.11604329566618077 1107 56-57 -0.3005650574674519 1107 58-59 -0.1504745776592742 1107 60-61 -0.2279603552924847 1107 62-63 -0.6187237457527637 1107 64-65 0.1699838178794657 1107 66-67 0.3252975442455721 1107 68-69 -0.1586932335416691 1107 70-71 -0.20344561875997513 1107 72-73 -0.5249783848662801 1107 74-75 -0.5584033194750226 1107 76-77 -0.27351037740139716 1107 78-79 -0.10950765347076796 1107 80-81 -0.3301138159974393 1107 82-83 -0.22632714407262355 1107 84-85 -0.09192642327298017 1107 86-87 -0.23345504468480627 1107 88-89 -0.275360452287412 1107 90-91 -0.41537411722978845 1107 92-93 -0.31623027137377946 1107 94-95 -0.7609466571136707 1107 96-97 -1.000407119689367 1107 98-99 -0.8489663253541408 1107 100-101 -0.2081486998258839 1108 1 0.15959080755489552 1108 2 0.14439243886293696 1108 3 0.3182710908606836 1108 4 0.19539045641835884 1108 5 0.281383932852286 1108 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90-91 -0.07949511153946176 2316 92-93 0.2364302058149974 2316 94-95 -0.0975244748235653 2316 96-97 -0.5089426093569358 2316 98-99 -0.05381807737570554 2316 100-101 0.37091688867366557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 20.0 6 56.0 7 88.0 8 152.0 9 327.0 10 546.0 11 797.0 12 1136.0 13 1362.0 14 1613.0 15 2141.0 16 2550.0 17 3357.0 18 2998.0 19 1912.0 20 1615.0 21 1689.0 22 1804.0 23 1751.0 24 2010.0 25 2214.0 26 2343.0 27 2700.0 28 3189.0 29 3849.0 30 4491.0 31 5358.0 32 6239.0 33 7652.0 34 10488.0 35 15126.0 36 19039.0 37 28487.0 38 23593.0 39 2072.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00754349677576 21.924808370848034 16.12544105122278 19.942207081153427 2 15.943436305152636 23.862353583783456 38.50397523821084 21.69023487285307 3 18.77465558050616 27.8521575529526 32.88887540207562 20.48431146446562 4 12.710444862535656 19.990896401043877 36.68689688656916 30.61176184985131 5 11.58294126574594 39.37289421763545 34.572772803156774 14.47139171346183 6 27.56680646003144 37.85041907663549 18.324664526270432 16.25810993706264 7 23.228136189840384 37.24889239546034 22.453116465375977 17.0698549493233 8 24.573648115555017 34.51963342841537 21.491776415609635 19.41494204041998 9 25.569581841354616 17.412150270073436 22.711658675729804 34.30660921284214 10-11 22.954724767858224 28.567396977605146 27.82848819566669 20.64939005886994 12-13 24.732354190690053 27.33719730533471 28.846574012259513 19.083874491715726 14-15 21.831947563270013 28.10948595011228 29.17612429447108 20.882442192146627 16-17 18.519597489773375 31.031887646723234 30.300547443040433 20.147967420462958 18-19 18.100685804454695 28.33768283061237 34.7960793833829 18.76555198155004 20-21 19.616131577350245 27.51289676518784 34.55270983795594 18.31826181950598 22-23 19.32633367724707 27.887358135582936 34.269891363719125 18.51641682345087 24-25 18.86519915760853 28.340282456044523 34.73742026716191 18.057098119185042 26-27 18.653613891131112 28.252016483883303 34.99942342823502 18.094946196750563 28-29 18.254233173514596 28.51429265036111 35.07980821751532 18.15166595860897 30-31 18.514292650361106 28.788007525641802 34.490805365054314 18.20689445894277 32-33 18.300661528190812 28.875098622322028 34.15639983006615 18.66784001942101 34-35 18.482967908352933 28.94105151354222 34.20104696153554 18.374933616569304 36-37 18.87747049968436 28.824721992910213 33.72414169863546 18.57366580876997 38-39 18.488787454817494 28.686755873346343 34.012752771648906 18.811703900187254 40-41 18.39411495145867 28.86957418978917 34.03082731182078 18.705483546931383 42-43 18.37105061105328 29.099307159353348 33.494884875558775 19.03475735403459 44-45 18.49772552780197 29.348429718960812 33.03705007480419 19.116794678433028 46-47 18.648228732346496 29.221819638402856 32.32040007525687 19.809551553993774 48-49 18.6066342369353 29.368530617570926 31.954311100860917 20.070524044632858 50-51 18.585660574317455 29.730927143676467 31.715214284197014 19.96819799780906 52-53 18.649025914911697 29.854038963403536 31.522121745463373 19.9748133762214 54-55 18.38471809188566 29.679553316744556 31.224737512896766 20.710991078473022 56-57 18.634382889881532 30.181224509613518 30.48073655078656 20.70365604971839 58-59 18.374704133033926 29.734478363779814 30.826303331917217 21.064514171269042 60-61 18.31953582093176 29.757107135053772 30.985227355490274 20.938129688524192 62-63 18.41291516834327 29.61111869755868 30.93237440636048 21.04359172773757 64-65 18.253710554026455 29.62040220554542 30.829082368078463 21.296804872349657 66-67 18.177293708139995 29.724964418049225 30.48515876074666 21.612583113064115 68-69 18.25221977435076 29.45669391693826 30.41530870491415 21.87577760379683 70-71 18.194756327001276 29.408872974449235 30.199672270437578 22.19669842811191 72-73 18.144079626145537 29.15093767069248 30.13169873156521 22.573283971596773 74-75 18.14802451902652 29.3412028888754 29.75086484190083 22.759907750197243 76-77 18.168458128377967 29.007408756907754 30.042812898524325 22.781320216189958 78-79 17.99846844820985 29.08125125350221 30.045764226110528 22.874516072177418 80-81 17.99450132611506 28.942688766561265 29.864596672877457 23.198213234446218 82-83 17.9920190571563 28.784499370324852 29.679093267786428 23.544388304732426 84-85 17.97748376524853 28.433270619651633 29.653759786368877 23.93548582873096 86-87 18.05169082712614 28.328796918478194 29.54998359599986 24.06952865839581 88-89 18.15707182505488 27.960891898557655 29.553959375561394 24.32807690082607 90-91 17.882021120089668 27.887716904295594 29.60711272474742 24.623149250867314 92-93 17.916793105541057 27.78327365418462 29.596103659646783 24.70382958062754 94-95 17.967166353098257 27.396370698549493 29.673484250773807 24.962978697578443 96-97 17.99781513625053 27.172118710930388 29.446197730169327 25.38386842264975 98-99 17.81452934393397 27.32475572009468 29.398859015597502 25.461855920373853 100-101 18.080021848969004 26.65968713490221 30.105148163321044 25.155142852807742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1593.0 1 1045.5 2 507.5 3 501.5 4 418.5 5 400.5 6 612.0 7 1003.5 8 1253.0 9 1237.5 10 1002.0 11 737.0 12 658.5 13 623.5 14 606.0 15 611.0 16 632.5 17 654.0 18 643.0 19 655.5 20 755.5 21 843.5 22 927.0 23 1037.0 24 1157.5 25 1379.5 26 1659.5 27 1932.0 28 2140.5 29 2302.5 30 2584.5 31 2930.0 32 3290.0 33 3543.0 34 3723.5 35 4010.5 36 4305.0 37 4570.5 38 4710.5 39 4719.5 40 4676.5 41 4652.5 42 4760.0 43 5012.5 44 5189.0 45 5249.0 46 5274.0 47 5455.0 48 5847.0 49 5852.5 50 5515.0 51 5111.0 52 4596.5 53 4190.5 54 3920.5 55 3596.5 56 3231.0 57 2812.0 58 2476.5 59 2243.0 60 1877.5 61 1504.0 62 1198.0 63 863.0 64 607.0 65 464.5 66 336.5 67 270.5 68 214.5 69 153.5 70 123.5 71 96.5 72 67.0 73 55.5 74 43.0 75 33.5 76 26.5 77 16.5 78 14.5 79 10.0 80 5.0 81 4.0 82 1.5 83 0.0 84 1.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23669357285913697 2 0.0 3 0.0 4 0.0 5 0.02731079686836196 6 0.0018207197912241306 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0024276263882988406 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.069065970747101E-4 26-27 0.0018207197912241306 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.004551799478060327 36-37 0.015779571523942465 38-39 0.01304849183710627 40-41 0.008193239060508587 42-43 0.008193239060508587 44-45 0.003944892880985616 46-47 6.069065970747101E-4 48-49 0.0021241730897614856 50-51 3.0345329853735507E-4 52-53 0.0 54-55 0.0 56-57 0.0012138131941494203 58-59 0.0 60-61 0.0036414395824482613 62-63 0.0015172664926867755 64-65 0.0021241730897614856 66-67 0.005765612672209747 68-69 6.069065970747101E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.06069065970747103 78-79 0.14019542392425804 80-81 0.0018207197912241306 82-83 0.0015172664926867755 84-85 0.0 86-87 0.10681556108514899 88-89 0.33895733446622567 90-91 0.3723371973053347 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0015172664926867755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 164770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.97166094813069 #Duplication Level Percentage of deduplicated Percentage of total 1 86.23070600783497 59.47475018090329 2 8.772036852270295 12.100439031985887 3 2.043092916394532 4.227465357452735 4 0.9371754554788158 2.585541910567793 5 0.5544239036048791 1.9119768750487411 6 0.33100513068149306 1.369798418727338 7 0.21734032936677325 1.0493226455208096 8 0.18344223004567872 1.0121852235423636 9 0.1216300557283819 0.7550124268320179 >10 0.5672744855218028 6.6534596471194085 >50 0.02392721889850091 1.1352688951553458 >100 0.011963609449250455 1.8325206575342197 >500 9.969674541042046E-4 0.37956900673879523 >1k 0.004984837270521023 5.512689722871235 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 1.2405170844207076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1870 1.1349153365297082 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1574 0.9552709837955938 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1361 0.8259998786186806 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1168 0.7088669053832615 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.33501244158524 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 382 0.2318383200825393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 377 0.22880378709716573 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 358 0.21727256175274626 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.17478909995751654 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 0.1292711051769133 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 201 0.12198822601201674 TruSeq Adapter, Index 1 (95% over 21bp) GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 186 0.11288462705589611 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 176 0.10681556108514899 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.069065970747103E-4 0.0 18-19 0.0 0.0 0.0 6.069065970747103E-4 0.0 20-21 0.0 0.0 0.0 0.0012138131941494205 0.0 22-23 0.0 0.0 0.0 0.0036414395824482613 0.0 24-25 0.0 0.0 0.0 0.014262305031255688 0.0 26-27 0.0 0.0 0.0 0.02791770346543667 0.0 28-29 0.0 0.0 0.0 0.05947684651332161 0.0 30-31 0.0 0.0 0.0 0.13625053104327245 0.0 32-33 0.0 0.0 0.0 0.28403228743096437 0.0 34-35 0.0 0.0 0.0 0.5404503246950294 0.0 36-37 0.0 0.0 0.0 0.8794076591612551 0.0 38-39 0.0 0.0 0.0 1.2881592522910723 0.0 40-41 0.0 0.0 0.0 1.718456029617042 0.0 42-43 0.0 0.0 0.0 2.1372215815985918 0.0 44-45 0.0 0.0 0.0 2.668871760636038 0.0 46-47 0.0 0.0 0.0 3.2436123080657886 3.034532985373551E-4 48-49 0.0 0.0 0.0 3.807731990046732 6.069065970747103E-4 50-51 0.0 0.0 0.0 4.378527644595497 6.069065970747103E-4 52-53 0.0 0.0 0.0 4.925957395156885 6.069065970747103E-4 54-55 0.0 0.0 0.0 5.547733203859925 6.069065970747103E-4 56-57 0.0 0.0 0.0 6.262972628512472 6.069065970747103E-4 58-59 0.0 0.0 0.0 7.0143229956909625 6.069065970747103E-4 60-61 0.0 0.0 0.0 7.786004733871457 6.069065970747103E-4 62-63 0.0 0.0 0.0 8.506402864599139 6.069065970747103E-4 64-65 0.0 0.0 0.0 9.292043454512351 6.069065970747103E-4 66-67 0.0 0.0 0.0 10.148995569581842 6.069065970747103E-4 68-69 0.0 0.0 0.0 11.068459064150026 6.069065970747103E-4 70-71 0.0 0.0 0.0 12.054985737694969 6.069065970747103E-4 72-73 0.0 0.0 0.0 13.004491108818353 6.069065970747103E-4 74-75 0.0 0.0 0.0 13.979486557018875 6.069065970747103E-4 76-77 0.0 0.0 0.0 14.985434241670207 6.069065970747103E-4 78-79 3.034532985373551E-4 0.0 0.0 16.147963828366812 6.069065970747103E-4 80-81 6.069065970747103E-4 0.0 0.0 17.332948959155186 6.069065970747103E-4 82-83 6.069065970747103E-4 0.0 0.0 18.527948048795288 6.069065970747103E-4 84-85 6.069065970747103E-4 0.0 0.0 19.725678218122233 6.069065970747103E-4 86-87 6.069065970747103E-4 0.0 0.0 20.955270983795593 6.069065970747103E-4 88-89 6.069065970747103E-4 0.0 0.0 22.236754263518847 6.069065970747103E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTACAC 50 0.0016195882 37.96273 3 GGTATCA 1405 0.0 36.94896 1 ACCAGTC 30 0.003965901 31.63561 62-63 GAGGGCG 60 0.003960196 31.63561 7 TATCCTC 60 0.003960196 31.63561 5 GTATCAA 1865 0.0 27.734169 2 GCCCCCT 35 0.008423258 27.116234 50-51 ATGGGAT 205 2.6619091E-8 23.148006 5 TATCAAC 2240 0.0 23.09117 3 ATCAACG 2310 0.0 22.39144 4 GAGTACT 1535 0.0 22.103708 12-13 AACGCAG 2345 0.0 22.057238 7 TCAACGC 2350 0.0 22.010307 5 CAACGCA 2355 0.0 21.963577 6 GTACTTT 1655 0.0 20.07093 14-15 ACGCAGA 2585 0.0 20.00937 8 CGCAGAG 2600 0.0 19.893932 9 CATGGGA 650 0.0 19.711418 4 ATGGGAG 220 1.3430781E-6 19.41276 5 GTACATG 1035 0.0 19.326845 1 >>END_MODULE