FastQCFastQC Report
Thu 26 May 2016
SRR938829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036243
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.489653488612227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41930.4046348202110895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26380.2545734928969363No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15670.15121935684969645No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14310.13809502211353902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.031.0599121
GTATCAA65900.028.6396351
CTAGTCG909.062497E-426.3309483
ATCACGC950.001242287924.9451083
CTATCGG1151.2962152E-424.741341
TATCGGA1351.3656212E-524.575552
ATCAACG76650.024.23893
TATCAAC76750.024.2073172
TCAACGC77350.023.958274
CAACGCA77450.023.866145
TATCACG1000.001674824323.6978552
ATCGGAC1401.8097837E-523.6978553
AACGCAG78750.023.454036
GTACATG34400.022.8833431
CCGATTA1050.002217446222.5813831
TAAGGTG3850.022.159035
GAGTACT47300.022.02855312-13
TACATGG33950.021.6386872
TAAGGCG4900.021.2797055
ACGCAGA87500.020.8931377
Produced by FastQC (version 0.11.5)