FastQCFastQC Report
Thu 26 May 2016
SRR938829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036243
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48100.46417683883027433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42010.40540683990145165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.2559245273550702No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT24610.23749255724767263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15030.14504319932679882No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14340.13838452949742483No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT11380.10981980095402333RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67050.027.9934961
GGTATCA47200.026.7462921
TATCAAC78300.023.7588442
TAGACTC2801.8189894E-1223.7285395
TCAACGC78250.023.7133754
ATCAACG78500.023.6378563
CAACGCA79150.023.5036955
AACGCAG80450.023.1828866
TCGCACG550.002893274221.571422-23
GAGTACT46300.021.16810412-13
AGTACTT46450.020.38450212-13
TAAGGTG4200.020.3387495
ACGCAGA91200.020.2941467
CGCAGAG91350.020.208878
GTACTTT49550.019.63600314-15
AGAGTAC68400.019.56658210-11
GCAGAGT95250.018.8831839
GTACCGC1300.007602165518.2527226
GTATAGG2352.4575093E-618.24461
GTTAGAC2101.9707662E-518.0788883
Produced by FastQC (version 0.11.5)