FastQCFastQC Report
Thu 26 May 2016
SRR938828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036866
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52280.5042117303489554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40610.39166102466471076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26420.2548063105550765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15360.14813871802142226No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13480.1300071561802586No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA600.003937592731.6882821
GGTATCA45350.029.6619071
GCGTATC650.00582434629.2507231
GTATCAA64100.028.030041
TAAGCGC700.00843670827.1127684
TCAACGC74450.024.090124
TATCAAC74900.024.072082
ATCAACG74450.024.026393
CAACGCA75050.023.8343095
AACGCAG76450.023.3955756
GTACATG35750.023.267621
GAGTACT44700.022.72242712-13
CTTAGCG1050.002210274322.5939713
TACATGG35100.022.4394822
ACATGGG35050.021.6592733
ACGCAGA83550.021.2370917
CGCAGAG84100.021.041798
GTACTTT48600.020.99820714-15
ATAGTAC2151.0901113E-619.861683
AGAGTAC61800.019.81242610-11
Produced by FastQC (version 0.11.5)