##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938828_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1036866 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61055334054738 33.0 31.0 34.0 30.0 34.0 2 31.67136351273935 33.0 31.0 34.0 30.0 34.0 3 31.68247970325963 33.0 31.0 34.0 30.0 34.0 4 35.24581672077202 37.0 35.0 37.0 33.0 37.0 5 35.27515416649789 37.0 35.0 37.0 33.0 37.0 6 35.33404702246963 37.0 35.0 37.0 33.0 37.0 7 35.25088005586064 37.0 35.0 37.0 33.0 37.0 8 35.22662330522941 37.0 35.0 37.0 33.0 37.0 9 36.81131505903367 39.0 37.0 39.0 33.0 39.0 10-11 36.702083972278004 39.0 37.0 39.0 32.0 39.0 12-13 36.61200338327228 39.0 37.0 39.0 32.0 39.0 14-15 37.781431737563004 40.0 38.0 41.0 32.0 41.0 16-17 37.678673039717765 40.0 38.0 41.0 32.0 41.0 18-19 37.637076054186366 40.0 37.0 41.0 32.0 41.0 20-21 37.53594485690533 40.0 37.0 41.0 32.0 41.0 22-23 37.50745274702806 40.0 37.0 41.0 32.0 41.0 24-25 37.43346729471311 40.0 37.0 41.0 32.0 41.0 26-27 37.282044642219915 40.0 37.0 41.0 31.5 41.0 28-29 37.10630351462966 40.0 37.0 41.0 31.0 41.0 30-31 36.85963036689408 39.5 36.0 41.0 30.5 41.0 32-33 36.63440984659542 39.0 35.5 41.0 30.0 41.0 34-35 36.31527410485057 39.0 35.0 41.0 30.0 41.0 36-37 36.08381073349884 39.0 35.0 41.0 29.5 41.0 38-39 35.89397762102335 39.0 35.0 41.0 29.0 41.0 40-41 35.75006944002407 39.0 35.0 41.0 28.0 41.0 42-43 35.709763846051466 39.0 35.0 41.0 27.5 41.0 44-45 35.49236015068485 39.0 35.0 41.0 26.5 41.0 46-47 35.146755704208644 39.0 35.0 41.0 24.5 41.0 48-49 34.814316411185246 39.0 35.0 41.0 23.5 41.0 50-51 33.64045643313601 38.0 33.0 40.0 21.5 40.5 52-53 33.685492146526165 38.0 33.5 39.5 22.0 40.5 54-55 34.174438162694116 38.0 34.0 40.0 22.0 41.0 56-57 34.05298466725691 38.0 34.0 40.0 20.5 41.0 58-59 33.83794723715504 38.0 34.0 40.0 20.0 41.0 60-61 33.527476549525204 37.5 33.0 40.0 18.5 41.0 62-63 33.12516901894748 37.0 33.0 40.0 15.5 41.0 64-65 32.72504258023699 36.5 32.5 39.5 12.5 41.0 66-67 32.34061055141166 36.0 32.0 39.0 8.0 41.0 68-69 31.895548219345606 35.0 31.5 39.0 7.0 40.5 70-71 31.457571663069288 35.0 31.0 38.5 2.0 40.0 72-73 31.01293416892829 35.0 31.0 37.5 2.0 40.0 74-75 30.534504940850603 35.0 30.5 37.0 2.0 39.0 76-77 30.06778166127542 35.0 30.0 36.0 2.0 39.0 78-79 29.600619559325892 34.0 29.5 36.0 2.0 38.5 80-81 29.10742516390739 34.0 29.0 35.5 2.0 37.0 82-83 28.675882418750348 34.0 29.0 35.0 2.0 37.0 84-85 28.305821099351313 34.0 28.5 35.0 2.0 36.5 86-87 27.85545528544672 34.0 27.0 35.0 2.0 36.0 88-89 27.36564175120025 34.0 26.5 35.0 2.0 36.0 90-91 26.88423528209045 34.0 25.0 35.0 2.0 35.0 92-93 26.380524580804078 33.0 24.5 35.0 2.0 35.0 94-95 25.824388590232488 33.0 22.5 35.0 2.0 35.0 96-97 25.20371774173326 33.0 18.5 35.0 2.0 35.0 98-99 24.447260301716906 32.5 2.0 35.0 2.0 35.0 100-101 22.14869616710356 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2642476875901991 1101 2 0.34647405391257635 1101 3 0.43711700377100726 1101 4 0.2518488284942677 1101 5 0.24890871386593716 1101 6 0.35756897685990197 1101 7 0.33506371289323766 1101 8 0.4670854034340266 1101 9 0.504871347019801 1101 10-11 0.31099438023166925 1101 12-13 0.2907748581619103 1101 14-15 0.3396438047152017 1101 16-17 0.41640647494499206 1101 18-19 0.5084490982703258 1101 20-21 0.6091126950749128 1101 22-23 0.6088800024715866 1101 24-25 0.5471086948482693 1101 26-27 0.569158242310003 1101 28-29 0.5901808776537578 1101 30-31 0.6471696735576131 1101 32-33 0.4832065796898988 1101 34-35 0.6382358741829037 1101 36-37 0.6058748291189744 1101 38-39 0.598123676363592 1101 40-41 0.6697071988904568 1101 42-43 0.41608884782458944 1101 44-45 0.30802754687687894 1101 46-47 0.1093410192918256 1101 48-49 0.06598202290191324 1101 50-51 0.21978201049724078 1101 52-53 0.3703048618241027 1101 54-55 0.23060048002796663 1101 56-57 0.3790580929899008 1101 58-59 0.19486259175916132 1101 60-61 0.16471552832472014 1101 62-63 0.2457822799897329 1101 64-65 -0.014048183462250563 1101 66-67 0.12907852380847373 1101 68-69 0.07902924058758032 1101 70-71 -0.004113374043861029 1101 72-73 0.13464603932722952 1101 74-75 -0.029198983787551924 1101 76-77 -0.004839315423247115 1101 78-79 -0.22783173603546558 1101 80-81 -0.21174433056020447 1101 82-83 -0.08222913506756058 1101 84-85 -0.27270719350034156 1101 86-87 -0.27994706148162507 1101 88-89 -0.11278580100592706 1101 90-91 -0.1984349064866997 1101 92-93 -0.268231271443355 1101 94-95 -0.24579931515622633 1101 96-97 -0.41725419467274705 1101 98-99 -0.286909129437781 1101 100-101 -0.014798704280529762 1102 1 0.251293394781797 1102 2 0.25775357939516397 1102 3 0.28815075065718787 1102 4 0.30321769112926944 1102 5 0.32346791088641424 1102 6 0.44429336241341133 1102 7 0.43924981978948097 1102 8 0.4503133876276024 1102 9 0.5038327316239517 1102 10-11 0.44616550834650326 1102 12-13 0.562739950947595 1102 14-15 0.5752360589163104 1102 16-17 0.7520577598258313 1102 18-19 0.6660397134065121 1102 20-21 0.6268746411805068 1102 22-23 0.6016228802476533 1102 24-25 0.6602264884396902 1102 26-27 0.6810589084844096 1102 28-29 0.7163330916085329 1102 30-31 0.6548396048304923 1102 32-33 0.6768251806425027 1102 34-35 0.5786605299386949 1102 36-37 0.6085170098867394 1102 38-39 0.7707123440959194 1102 40-41 0.7005989070070697 1102 42-43 0.5316946527109536 1102 44-45 0.7445482664425995 1102 46-47 0.8110291750192218 1102 48-49 0.6827988695681952 1102 50-51 0.6881072837423474 1102 52-53 0.3802295216369984 1102 54-55 0.3912602848007154 1102 56-57 0.39076483019587016 1102 58-59 0.12866708863925425 1102 60-61 0.20054978802725287 1102 62-63 0.0334645448204256 1102 64-65 0.03646970425368323 1102 66-67 -0.010501648374486194 1102 68-69 0.15425380059600968 1102 70-71 -0.03917070074520268 1102 72-73 -0.15480714589521938 1102 74-75 -0.1998676330195437 1102 76-77 -0.3491240907820945 1102 78-79 -0.46013977475634604 1102 80-81 -0.5136101868904852 1102 82-83 -0.5523577854355359 1102 84-85 -0.5800531660257207 1102 86-87 -0.6113130435817418 1102 88-89 -0.48835075098199 1102 90-91 -0.6123119847317646 1102 92-93 -0.8207766905814644 1102 94-95 -0.7482345842630949 1102 96-97 -0.7168024375464626 1102 98-99 -1.0342243492104224 1102 100-101 -0.745720232018865 1103 1 0.04401386100543547 1103 2 0.1849181605073369 1103 3 0.22087658775146934 1103 4 0.2727420083473149 1103 5 0.3066318346681456 1103 6 0.2130132514688583 1103 7 0.28545854625183864 1103 8 0.32459092536397804 1103 9 0.1916047822544229 1103 10-11 0.1855390002942059 1103 12-13 0.3160005504008794 1103 14-15 0.2569849186083317 1103 16-17 0.15693723588537978 1103 18-19 0.5493605547894802 1103 20-21 0.3543109723147282 1103 22-23 0.4206856633205973 1103 24-25 0.353439551679692 1103 26-27 0.3131302208205753 1103 28-29 0.4021381610691179 1103 30-31 0.32050090329858705 1103 32-33 0.13615686797766813 1103 34-35 0.17277201157753552 1103 36-37 0.4904931295529451 1103 38-39 0.45258445324483887 1103 40-41 0.48840041726383276 1103 42-43 0.5230862292618923 1103 44-45 0.39757399050811415 1103 46-47 0.3798705098809805 1103 48-49 0.30735797720205227 1103 50-51 0.3104799660513393 1103 52-53 0.3952990223063466 1103 54-55 0.34344250160753376 1103 56-57 0.33666934092558165 1103 58-59 0.44982310842330264 1103 60-61 0.34578975371780984 1103 62-63 0.08504494354745162 1103 64-65 -0.23402076846308972 1103 66-67 0.0517663821192329 1103 68-69 0.00404778361765068 1103 70-71 0.06295399964839277 1103 72-73 0.08259134865775764 1103 74-75 0.11369401575758076 1103 76-77 -0.05481841609653415 1103 78-79 0.07220268638500471 1103 80-81 0.12734109851117026 1103 82-83 0.05975412907944033 1103 84-85 -0.0182671110504522 1103 86-87 -0.03540240044002019 1103 88-89 -0.04390920128540543 1103 90-91 0.09663592583923375 1103 92-93 -0.1842303207288829 1103 94-95 -0.042869926195166386 1103 96-97 -0.4591399286284137 1103 98-99 -0.35260575317204257 1103 100-101 0.05054140152743258 1104 1 -0.21626719290697238 1104 2 -0.015646889874002312 1104 3 0.026190685778363587 1104 4 0.11392348455635215 1104 5 0.17029404508479473 1104 6 0.21357591599355885 1104 7 0.1233356110119459 1104 8 0.10186921963553885 1104 9 0.11853991081233772 1104 10-11 0.05085938107692556 1104 12-13 0.23389328228626738 1104 14-15 0.20485395098440762 1104 16-17 0.2308520687561213 1104 18-19 0.15831566887318615 1104 20-21 0.35102743711141215 1104 22-23 0.1284818475405345 1104 24-25 -0.04604132567932595 1104 26-27 0.21987006701234435 1104 28-29 0.09187440461736429 1104 30-31 0.18000377030298154 1104 32-33 0.06826731723447921 1104 34-35 0.12052075701458165 1104 36-37 0.1325808336431038 1104 38-39 0.42750611761843516 1104 40-41 0.21042127760373575 1104 42-43 0.1943877538242873 1104 44-45 0.4090877370188011 1104 46-47 0.4090107664400975 1104 48-49 0.33680143637606363 1104 50-51 0.5781164171691202 1104 52-53 0.3978349890952231 1104 54-55 0.4232016891438235 1104 56-57 0.4558011276936256 1104 58-59 0.35971129127534596 1104 60-61 0.33464581498660806 1104 62-63 0.37307251609737335 1104 64-65 0.30968514289438787 1104 66-67 0.23790197035219052 1104 68-69 0.36625362737585476 1104 70-71 0.4127961354672607 1104 72-73 0.37218961016355223 1104 74-75 0.2522625813010393 1104 76-77 0.22446101543335928 1104 78-79 0.043003777516776864 1104 80-81 0.09766998884924405 1104 82-83 0.05569419636059436 1104 84-85 0.2184327322782984 1104 86-87 0.27607416647132865 1104 88-89 0.34463351747776727 1104 90-91 0.3707360602424892 1104 92-93 0.03240347413516176 1104 94-95 0.020040142915149772 1104 96-97 -0.07470088328918578 1104 98-99 0.09104925991040602 1104 100-101 0.6409524448625419 1105 1 0.20207440998597193 1105 2 0.3440158802883069 1105 3 0.3690129883601472 1105 4 0.33211371245980814 1105 5 0.4049042544868655 1105 6 0.27957120654944134 1105 7 0.21643122954696992 1105 8 0.27517255829502574 1105 9 0.33174737864514725 1105 10-11 0.33996458669462015 1105 12-13 0.4256769842457331 1105 14-15 0.5655889606555817 1105 16-17 0.4446587292556501 1105 18-19 0.5784548497663025 1105 20-21 0.6464924947180037 1105 22-23 0.5547706373815942 1105 24-25 0.6121980990957141 1105 26-27 0.541951893603887 1105 28-29 0.507850773299559 1105 30-31 0.6364624365211355 1105 32-33 0.45658621387899956 1105 34-35 0.5586987908216656 1105 36-37 0.725487233441271 1105 38-39 0.7379825283492352 1105 40-41 0.5993183426108786 1105 42-43 0.5250895450949926 1105 44-45 0.5474276579674111 1105 46-47 0.6700380139382034 1105 48-49 0.5252212496802429 1105 50-51 0.6330634359227929 1105 52-53 0.5635590661282706 1105 54-55 0.5016013191160695 1105 56-57 0.45247928764652556 1105 58-59 0.525487929068646 1105 60-61 0.5761730204361086 1105 62-63 0.42532842392682824 1105 64-65 0.5883097329204574 1105 66-67 0.39502710701016497 1105 68-69 0.490244460779266 1105 70-71 0.3577965559533425 1105 72-73 0.5565832692334354 1105 74-75 0.3532843624800819 1105 76-77 0.4805475514690123 1105 78-79 0.6661221023002248 1105 80-81 0.6427566089821184 1105 82-83 0.41348580345840347 1105 84-85 0.5468849230107828 1105 86-87 0.7838846954428185 1105 88-89 0.9682984741676997 1105 90-91 0.9084011010296358 1105 92-93 0.9338835851434872 1105 94-95 0.8954816533011538 1105 96-97 1.1334397272566044 1105 98-99 1.288709254864571 1105 100-101 1.5178656565352355 1106 1 0.11997972329325535 1106 2 0.13652039134201033 1106 3 0.18603667579651884 1106 4 0.21668080893608277 1106 5 0.23580418420159077 1106 6 0.32097646700367477 1106 7 0.1805607028153844 1106 8 0.2543169324129835 1106 9 0.21886903893545195 1106 10-11 0.2792860463287994 1106 12-13 0.3093937957791937 1106 14-15 0.348757027773388 1106 16-17 0.32271377431055726 1106 18-19 0.32305642126349454 1106 20-21 0.3914195296993057 1106 22-23 0.34042557932669837 1106 24-25 0.28289210593137426 1106 26-27 0.3334197431896868 1106 28-29 0.3506648919240476 1106 30-31 0.2635619541675638 1106 32-33 0.31781730037068456 1106 34-35 0.24190830585325784 1106 36-37 0.28064680327312175 1106 38-39 0.33313284426208156 1106 40-41 0.4956215966893822 1106 42-43 0.7162789425888363 1106 44-45 0.5718545733339297 1106 46-47 0.36935101708527895 1106 48-49 0.1874337337547871 1106 50-51 0.2742626622284021 1106 52-53 0.09905173995452543 1106 54-55 0.06409865328269149 1106 56-57 0.1789782278039027 1106 58-59 0.21314127244096426 1106 60-61 0.3154229893733529 1106 62-63 0.2699395460618703 1106 64-65 0.10577302787196885 1106 66-67 0.16573205535326707 1106 68-69 0.31229660540745385 1106 70-71 0.3634212325761652 1106 72-73 0.27712094232715856 1106 74-75 0.1507046702887962 1106 76-77 0.35039136608649457 1106 78-79 0.46849993295165504 1106 80-81 0.32309954178828093 1106 82-83 0.3099499347861645 1106 84-85 0.3543477728840294 1106 86-87 0.4082696992723456 1106 88-89 0.664839937844679 1106 90-91 0.7505763468890159 1106 92-93 0.9559868210479472 1106 94-95 1.0082691228642915 1106 96-97 0.996040820294656 1106 98-99 0.8859936283520007 1106 100-101 1.2182895008616939 1107 1 0.15487905215231734 1107 2 0.2985331839815153 1107 3 0.3240288345656879 1107 4 0.2780404657584512 1107 5 0.22936825317921006 1107 6 0.17907629640514244 1107 7 0.14675968642622195 1107 8 0.17811205212454695 1107 9 0.21346930271049303 1107 10-11 0.21488172329890176 1107 12-13 0.24333120097097094 1107 14-15 0.15000973416835706 1107 16-17 0.358446290211333 1107 18-19 0.28108423017512507 1107 20-21 0.24693337338783294 1107 22-23 0.025984120731727955 1107 24-25 -0.04914260794472369 1107 26-27 -0.006453950876270653 1107 28-29 0.17547820131056824 1107 30-31 0.15899855588074985 1107 32-33 0.3737155534236791 1107 34-35 0.37796986536694277 1107 36-37 0.2861001047373861 1107 38-39 0.37220215948225643 1107 40-41 0.34383301283727263 1107 42-43 0.21342828157577998 1107 44-45 0.643180965692558 1107 46-47 0.5667920390560326 1107 48-49 0.25702263179628204 1107 50-51 0.28693871856642517 1107 52-53 0.335607540079657 1107 54-55 0.16547469476649468 1107 56-57 0.23405364676059293 1107 58-59 0.28416247797393623 1107 60-61 0.14139672890225086 1107 62-63 0.08242924938246432 1107 64-65 0.09176729324046562 1107 66-67 0.01836597970474685 1107 68-69 0.057267099513026665 1107 70-71 0.027943939286224406 1107 72-73 -0.12217055610414818 1107 74-75 -0.01202697686481713 1107 76-77 -0.0716527554660864 1107 78-79 -0.13765646854457358 1107 80-81 -0.2612571576927216 1107 82-83 -0.2610708206187482 1107 84-85 -0.2253428991121531 1107 86-87 -0.1211502206948829 1107 88-89 -0.024995720400092125 1107 90-91 0.16850044629530103 1107 92-93 0.302758277527321 1107 94-95 0.19649962444199076 1107 96-97 0.39008877693832034 1107 98-99 0.50279330786082 1107 100-101 0.8460142385495644 1108 1 0.30022318170697204 1108 2 0.3573108160356462 1108 3 0.04139687546967963 1108 4 0.11812809001953894 1108 5 0.26377550641522873 1108 6 0.22159307046379695 1108 7 0.07225625558476167 1108 8 0.2754662176032454 1108 9 0.2850398868088746 1108 10-11 0.45739992291495213 1108 12-13 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2316 74-75 -0.719447809047459 2316 76-77 -0.7534349519127375 2316 78-79 -0.6351928305071972 2316 80-81 -0.5992496551611914 2316 82-83 -0.35845529083876215 2316 84-85 -0.35263278220153893 2316 86-87 -0.4181054816725158 2316 88-89 -0.548438830038311 2316 90-91 -0.8167833150965826 2316 92-93 -0.42371461209801353 2316 94-95 -0.5386057792218253 2316 96-97 -0.3059068043928015 2316 98-99 -0.49062586732098623 2316 100-101 -0.844290005213665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6858.0 3 1304.0 4 1113.0 5 1070.0 6 1287.0 7 1635.0 8 1930.0 9 2443.0 10 2798.0 11 3789.0 12 5161.0 13 6702.0 14 7089.0 15 8013.0 16 9239.0 17 11558.0 18 10143.0 19 7641.0 20 7534.0 21 8030.0 22 8709.0 23 9743.0 24 11135.0 25 12589.0 26 14383.0 27 16966.0 28 19662.0 29 23190.0 30 27265.0 31 32784.0 32 40012.0 33 49839.0 34 64622.0 35 90350.0 36 130350.0 37 188803.0 38 164508.0 39 26617.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19612801531774 19.499949230970078 14.422175912271115 22.881746841441068 2 15.903810689482953 21.521648197827016 38.96888134957934 23.605659763110687 3 18.718104078909096 25.149906601302074 31.396457903907088 24.735531415881738 4 13.121069562109373 17.325998125947894 37.42308175310812 32.12985055883461 5 13.322873254196358 37.16750337632406 34.813695549826036 14.695927819653543 6 30.57929504350414 36.39901279806425 17.90376133987345 15.11793081855816 7 26.39714832371205 33.72433989243372 22.31318745595013 17.5653243279041 8 25.132193537720482 33.99092507920977 21.374696769440355 19.502184613629396 9 25.693648932409435 16.820950390313353 21.85600228721113 35.629398390066086 10-11 24.144776071802376 26.493651961044872 28.452908925912002 20.90866304124075 12-13 25.13807655933849 24.702233532416155 28.46974590240383 21.689944005841525 14-15 22.45531254649408 26.13889967712578 27.29625206989232 24.10953570648782 16-17 21.138915985516295 28.845728811149378 28.19955283713377 21.81580236620056 18-19 20.981808180880922 27.452067454300526 30.714578831135626 20.851545533682923 20-21 21.869733281793582 26.763335910320833 30.514592191727868 20.852338616157713 22-23 21.609604354541258 26.69878108616993 30.415465195816587 21.276149363472225 24-25 21.611804646612676 26.821167085881324 30.470654651542716 21.09637361596328 26-27 21.282064681961323 26.792070469233582 30.62938037475975 21.29648447404534 28-29 21.179256988858754 27.344893993420033 30.397066926343726 21.078782091377494 30-31 21.39749100077043 26.960835759163544 30.106926758869978 21.53474648119605 32-33 21.1216521901298 27.125364651224814 30.08586394921987 21.667119209425515 34-35 21.927402961959153 27.05493175878424 29.714451650372663 21.30321362888394 36-37 21.857545415811288 26.70193089824534 29.965139249331834 21.475384436611535 38-39 21.45969098145676 26.99327997459538 29.917522120123962 21.6295069238239 40-41 21.68704957467122 26.980782659148066 29.84671324096222 21.485454525218493 42-43 21.498449069054185 27.05633887357105 29.43181906192881 22.013392995445955 44-45 21.452500065354226 27.069381013193805 29.590553361482446 21.88756555996952 46-47 21.858598631755832 27.19994074266291 29.06891909098083 21.872541534600426 48-49 21.558281198551203 27.28844254198222 28.964535435511053 22.18874082395553 50-51 21.418721644692564 27.661755378127257 28.908347899346833 22.011175077833354 52-53 21.736360062207915 27.484201087282816 28.636606608525895 22.14283224198337 54-55 21.382001952141547 27.33622733153096 28.805804522461248 22.47596619386625 56-57 21.35719682979639 27.593628032089285 28.58846747191607 22.460707666198257 58-59 21.55680914664451 27.18248332017853 28.697132575726414 22.563574957450548 60-61 21.27777947102111 27.132918150068768 29.139605009871612 22.449697369038514 62-63 21.200121412156996 27.09974531524802 29.123620134860438 22.57651313773454 64-65 21.04183514849807 27.182580229635693 28.89002958823444 22.8855550336318 66-67 21.01684753340643 27.14332898116687 28.670736006880816 23.169087478545887 68-69 21.119315845577074 26.906712863298427 28.818523079992854 23.155448211131652 70-71 21.230651208135633 26.792709971411178 28.52963953287327 23.446999287579917 72-73 21.095930346586652 26.669142896714572 28.755326356304966 23.479600400393807 74-75 20.84112362238393 27.19659517759922 28.454857000074092 23.50742419994276 76-77 20.959721892717596 26.62945012806298 28.688383307753014 23.722444671466405 78-79 21.009334846787016 26.77815883034878 28.71754162199316 23.494964700871044 80-81 21.152796960239417 26.572489856895203 28.55008667810111 23.72462650476427 82-83 21.048681994299702 26.261203665734367 28.823915871502738 23.86619846846319 84-85 20.921337706822886 26.250136167225456 28.78592091407545 24.042605211876204 86-87 21.031821533334206 26.109643383290948 28.79376979798611 24.06476528538874 88-89 21.04499702963538 25.95541886982831 28.64259420558603 24.356989894950285 90-91 20.915345393757164 25.84166226390333 28.820755150990973 24.422237191348533 92-93 21.16032030744556 25.68323925819461 28.750188860522147 24.406251573837682 94-95 20.998025510003938 25.27134103079323 28.911773665427344 24.818859793775488 96-97 20.982542194016197 24.9362289066242 29.124707539251855 24.956521360107747 98-99 20.99735626155334 25.038028862708178 28.839268547939128 25.125346327799353 100-101 21.225472561853906 24.41702321739564 29.288643167322796 25.068861053427653 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1852.0 1 1236.5 2 656.0 3 732.0 4 692.0 5 671.5 6 934.5 7 1423.0 8 1986.0 9 2182.5 10 1880.5 11 1627.0 12 1649.5 13 1743.5 14 1855.5 15 1953.5 16 1995.0 17 2114.5 18 2341.5 19 2510.0 20 2754.5 21 3222.5 22 3680.5 23 4269.0 24 5008.5 25 5845.0 26 7326.5 27 9119.0 28 10461.5 29 11774.5 30 13310.5 31 15330.5 32 17899.0 33 19367.5 34 19956.5 35 21446.0 36 23793.5 37 25679.5 38 26896.5 39 27898.5 40 28891.0 41 30124.5 42 31066.5 43 32174.0 44 33797.0 45 35540.5 46 36871.0 47 38599.5 48 41234.5 49 41485.0 50 39188.0 51 37237.5 52 35587.0 53 34771.0 54 33960.5 55 31209.0 56 28079.5 57 25072.0 58 22432.0 59 19940.5 60 16633.0 61 13416.5 62 10884.0 63 8372.0 64 5962.0 65 4333.0 66 3161.5 67 2418.5 68 1901.0 69 1492.5 70 1203.5 71 866.5 72 623.5 73 508.5 74 394.0 75 311.5 76 240.0 77 158.0 78 119.5 79 103.0 80 77.5 81 62.5 82 51.5 83 37.0 84 31.0 85 20.0 86 11.5 87 11.5 88 8.5 89 6.5 90 7.0 91 7.5 92 6.0 93 3.0 94 3.5 95 3.0 96 1.5 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26724764820140695 2 0.16385916791562266 3 0.19819340204037936 4 0.16164094492441647 5 0.2369640821475485 6 0.19501073427038787 7 0.2437151955990456 8 0.24950186427175738 9 0.1489102738444505 10-11 0.20610184922641886 12-13 0.21261185148321962 14-15 0.17244272644681183 16-17 0.290731878564829 18-19 0.23315452527134656 20-21 0.19925429129704322 22-23 0.24530652948404133 24-25 0.23069519108544403 26-27 0.3437281191590813 28-29 0.31580744281324685 30-31 0.2919856567772499 32-33 0.1902849548543399 34-35 0.3632099036905444 36-37 0.403716584399527 38-39 0.3841383553901854 40-41 0.3478270094689188 42-43 0.3797983538856516 44-45 0.38896057928411193 46-47 0.3937345809390992 48-49 0.41336103218738 50-51 0.4061276963464903 52-53 0.4050668070898264 54-55 0.4006303611074141 56-57 0.37955724269095525 58-59 0.3526492333628453 60-61 0.322751445220501 62-63 0.3877550233106303 64-65 0.4205943680282698 66-67 0.4272490370018884 68-69 0.4308657049223332 70-71 0.4310585938780903 72-73 0.4226679243026582 74-75 0.41765281145297467 76-77 0.4022699172313491 78-79 0.3769532417882349 80-81 0.3878514677885088 82-83 0.3966279152754551 84-85 0.3981710269215116 86-87 0.3945061367621274 88-89 0.40164302812513863 90-91 0.4215105905681159 92-93 0.4203050345946342 94-95 0.4289368153647627 96-97 0.43038348253294056 98-99 0.4269114813293135 100-101 0.4022216949924098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1036866.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.80191339835434 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27183340084261 53.313722231652385 2 10.804559615438524 14.003122730140078 3 2.962893699833487 5.760035428354178 4 1.2815281562904852 3.321819064059549 5 0.6825159326062155 2.2114169178872505 6 0.4483862320289414 1.7433771466172323 7 0.2939874501292597 1.3335664498435509 8 0.21044669031407276 1.0909878560562272 9 0.15363843493072554 0.8960458099535615 >10 0.8197408783485843 9.309958484682042 >50 0.04390406313595633 1.908921791269333 >100 0.024326786001400563 2.9599981123493664 >500 0.0014924400665225995 0.714225297799289 >1k 5.969760266090399E-4 0.9271872620178447 >5k 1.4924400665225997E-4 0.5056154173181696 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5228 0.5042117303489554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4061 0.39166102466471076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2642 0.2548063105550765 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1536 0.14813871802142226 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1348 0.1300071561802586 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.644447787853011E-5 7 0.0 0.0 0.0 0.0 9.644447787853011E-5 8 0.0 0.0 0.0 0.0 9.644447787853011E-5 9 0.0 0.0 0.0 0.0 9.644447787853011E-5 10-11 0.0 0.0 0.0 0.0 9.644447787853011E-5 12-13 0.0 0.0 0.0 0.0 9.644447787853011E-5 14-15 0.0 0.0 0.0 0.0 9.644447787853011E-5 16-17 0.0 0.0 0.0 0.0 9.644447787853011E-5 18-19 0.0 0.0 0.0 0.0 9.644447787853011E-5 20-21 0.0 9.644447787853011E-5 0.0 4.340001504533855E-4 9.644447787853011E-5 22-23 0.0 9.644447787853011E-5 0.0 8.680003009067709E-4 9.644447787853011E-5 24-25 0.0 9.644447787853011E-5 0.0 0.003520223442566349 9.644447787853011E-5 26-27 0.0 9.644447787853011E-5 0.0 0.007763780469221673 1.9288895575706022E-4 28-29 0.0 9.644447787853011E-5 0.0 0.02155534080585148 1.9288895575706022E-4 30-31 0.0 9.644447787853011E-5 0.0 0.057529131054543214 1.9288895575706022E-4 32-33 0.0 9.644447787853011E-5 0.0 0.1302482673749549 2.8933343363559034E-4 34-35 0.0 9.644447787853011E-5 0.0 0.25273275428068814 5.786668672711807E-4 36-37 0.0 9.644447787853011E-5 0.0 0.44648971033865514 5.786668672711807E-4 38-39 0.0 9.644447787853011E-5 0.0 0.6972935750617727 5.786668672711807E-4 40-41 0.0 9.644447787853011E-5 0.0 0.9721603370155835 5.786668672711807E-4 42-43 0.0 9.644447787853011E-5 0.0 1.256044657651037 5.786668672711807E-4 44-45 0.0 9.644447787853011E-5 0.0 1.595529219783463 5.786668672711807E-4 46-47 0.0 9.644447787853011E-5 0.0 1.996690025519209 6.751113451497107E-4 48-49 0.0 9.644447787853011E-5 0.0 2.3938001631840566 7.715558230282409E-4 50-51 0.0 9.644447787853011E-5 0.0 2.822640534070941 7.715558230282409E-4 52-53 0.0 9.644447787853011E-5 0.0 3.231131120125455 9.644447787853011E-4 54-55 0.0 9.644447787853011E-5 0.0 3.685722166605907 0.0010608892566638312 56-57 0.0 9.644447787853011E-5 0.0 4.222291019283109 0.0010608892566638312 58-59 0.0 9.644447787853011E-5 0.0 4.806503444032305 0.0010608892566638312 60-61 0.0 9.644447787853011E-5 0.0 5.416562988852947 0.0010608892566638312 62-63 0.0 9.644447787853011E-5 0.0 6.020932309478756 0.0010608892566638312 64-65 0.0 9.644447787853011E-5 0.0 6.665229643946276 0.0010608892566638312 66-67 0.0 9.644447787853011E-5 0.0 7.383355226229812 0.0011091114956030962 68-69 0.0 9.644447787853011E-5 0.0 8.179793724550713 0.0011573337345423614 70-71 0.0 9.644447787853011E-5 0.0 9.024647350766637 0.0012055559734816266 72-73 0.0 9.644447787853011E-5 0.0 9.862894530247882 0.0013502226902994217 74-75 0.0 9.644447787853011E-5 0.0 10.715608381410906 0.0014466671681779516 76-77 0.0 9.644447787853011E-5 0.0 11.633470477380875 0.0014948894071172168 78-79 0.0 9.644447787853011E-5 0.0 12.63837371463622 0.0015431116460564818 80-81 0.0 9.644447787853011E-5 0.0 13.689329190078563 0.0015913338849957467 82-83 0.0 9.644447787853011E-5 0.0 14.76309378453918 0.001639556123935012 84-85 0.0 9.644447787853011E-5 0.0 15.850023050230213 0.001639556123935012 86-87 0.0 9.644447787853011E-5 0.0 16.94331765146123 0.001639556123935012 88-89 0.0 1.4466671681779517E-4 0.0 18.10253205332222 0.001639556123935012 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCGA 60 0.0039375927 31.688282 1 GGTATCA 4535 0.0 29.661907 1 GCGTATC 65 0.005824346 29.250723 1 GTATCAA 6410 0.0 28.03004 1 TAAGCGC 70 0.008436708 27.112768 4 TCAACGC 7445 0.0 24.09012 4 TATCAAC 7490 0.0 24.07208 2 ATCAACG 7445 0.0 24.02639 3 CAACGCA 7505 0.0 23.834309 5 AACGCAG 7645 0.0 23.395575 6 GTACATG 3575 0.0 23.26762 1 GAGTACT 4470 0.0 22.722427 12-13 CTTAGCG 105 0.0022102743 22.593971 3 TACATGG 3510 0.0 22.439482 2 ACATGGG 3505 0.0 21.659273 3 ACGCAGA 8355 0.0 21.237091 7 CGCAGAG 8410 0.0 21.04179 8 GTACTTT 4860 0.0 20.998207 14-15 ATAGTAC 215 1.0901113E-6 19.86168 3 AGAGTAC 6180 0.0 19.812426 10-11 >>END_MODULE