FastQCFastQC Report
Thu 26 May 2016
SRR938828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036866
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47980.46274060486118745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.4081530303819394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.2612680905729381No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT23720.22876630152787342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14780.14254493830446752No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14610.1409053821805325No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10550.10174892416184927RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48150.026.8817211
GTATCAA67000.026.7763271
TATCAAC77750.023.0118062
ATCAACG78900.022.495953
CAACGCA79800.022.2422375
TCAACGC79850.022.2283124
AACGCAG81350.021.8131856
CCGTTAT1553.9399783E-521.4274949
GAGTACT46900.021.09401112-13
GTACTAG1200.004713270319.827741
TACATGG33850.019.7683412
ACGCAGA89450.019.6787997
GTACATG34600.019.6672841
CGCAGAG89450.019.519678
AGAGTAC67100.019.48161910-11
GTATAAT4700.019.2371251
AGTACTT49150.019.11470812-13
ACCGCTA757.775005E-418.98138260-61
GTACTTT52500.018.88917414-15
GCAGAGT93900.018.1903889
Produced by FastQC (version 0.11.5)