##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938828_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1036866 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01425160049611 31.0 31.0 34.0 30.0 34.0 2 32.22033319638218 34.0 31.0 34.0 30.0 34.0 3 32.294033173042614 34.0 31.0 34.0 30.0 34.0 4 35.940417566011426 37.0 35.0 37.0 35.0 37.0 5 35.83949709991455 37.0 35.0 37.0 35.0 37.0 6 35.86059722278482 37.0 35.0 37.0 35.0 37.0 7 35.77435657066583 37.0 35.0 37.0 33.0 37.0 8 35.76591092773801 37.0 35.0 37.0 33.0 37.0 9 37.40017610761661 39.0 37.0 39.0 34.0 39.0 10-11 37.33781559044274 39.0 37.0 39.0 34.0 39.0 12-13 37.26548946536968 39.0 37.0 39.0 33.0 39.0 14-15 38.47315805513924 40.0 38.0 41.0 33.5 41.0 16-17 38.41000090657809 40.0 38.0 41.0 33.0 41.0 18-19 38.52732368502777 40.0 38.0 41.0 34.0 41.0 20-21 38.51455347171187 40.0 38.0 41.0 34.0 41.0 22-23 38.43164545852598 40.0 38.0 41.0 34.0 41.0 24-25 38.33862717072409 40.0 38.0 41.0 33.5 41.0 26-27 38.142289842660475 40.0 37.5 41.0 33.0 41.0 28-29 37.95001138044839 40.0 37.0 41.0 33.0 41.0 30-31 37.699645855877236 40.0 37.0 41.0 32.5 41.0 32-33 37.50209043405802 40.0 36.0 41.0 32.0 41.0 34-35 37.43146317846279 40.0 36.0 41.0 32.0 41.0 36-37 37.274751028580354 40.0 36.0 41.0 31.5 41.0 38-39 37.11737341180056 40.0 36.0 41.0 31.0 41.0 40-41 36.91451595480997 40.0 36.0 41.0 31.0 41.0 42-43 36.67704119915206 39.5 35.0 41.0 30.0 41.0 44-45 36.39703539319449 39.0 35.0 41.0 30.0 41.0 46-47 36.03444466305193 39.0 35.0 41.0 29.5 41.0 48-49 35.67533316744883 39.0 35.0 41.0 27.5 41.0 50-51 35.393785214289984 39.0 35.0 40.5 27.0 41.0 52-53 35.163770921218365 38.5 35.0 40.0 26.5 41.0 54-55 34.88295932164812 38.0 34.0 40.0 26.0 41.0 56-57 34.57308128533485 38.0 34.0 40.0 25.0 41.0 58-59 34.23358514986508 38.0 34.0 40.0 24.0 41.0 60-61 33.83833156839938 37.0 33.0 40.0 23.0 41.0 62-63 33.45374667507662 37.0 33.0 40.0 22.0 41.0 64-65 33.03823637769972 36.0 32.5 39.0 21.5 41.0 66-67 32.611344185265985 36.0 32.0 39.0 19.5 40.0 68-69 32.26506752077896 35.0 32.0 38.5 18.5 40.0 70-71 31.83419988696707 35.0 31.0 38.0 16.0 40.0 72-73 31.366082502464156 35.0 31.0 37.0 12.0 39.5 74-75 30.888742614764105 35.0 31.0 37.0 7.5 39.0 76-77 28.94565787671695 32.5 28.0 35.0 6.5 37.0 78-79 29.875703803577316 34.0 29.5 35.5 2.0 37.5 80-81 29.9348676685319 34.0 30.0 35.0 2.0 37.0 82-83 29.634408882150634 34.0 30.0 35.0 2.0 37.0 84-85 29.254021252505147 34.0 29.5 35.0 2.0 36.0 86-87 28.713262369486507 34.0 29.0 35.0 2.0 36.0 88-89 28.142808713951467 34.0 28.0 35.0 2.0 35.5 90-91 27.642666940568986 33.0 27.0 35.0 2.0 35.0 92-93 27.3608706428796 33.0 26.5 35.0 2.0 35.0 94-95 27.046550373915238 33.0 26.0 35.0 2.0 35.0 96-97 26.48124781794369 33.0 25.0 35.0 2.0 35.0 98-99 25.718133780064157 33.0 23.5 35.0 2.0 35.0 100-101 22.397272164387683 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0765918391207947 1101 2 0.11174293109473865 1101 3 0.053097112829561866 1101 4 0.13277844480322187 1101 5 0.10062511557784859 1101 6 0.08147939907057378 1101 7 0.1948898069786722 1101 8 0.15003568606289264 1101 9 0.2415630210468933 1101 10-11 0.2173646824493929 1101 12-13 0.11383979010631151 1101 14-15 0.2323999194268751 1101 16-17 0.31296435248430754 1101 18-19 0.3099305048937353 1101 20-21 0.25436851079715694 1101 22-23 -0.018369786254325504 1101 24-25 0.01960602733183947 1101 26-27 -0.07316564230288236 1101 28-29 0.09805415477094925 1101 30-31 -0.029220283628063726 1101 32-33 0.17336606097758533 1101 34-35 0.08771339183402205 1101 36-37 -0.042438733427452746 1101 38-39 0.17538197849441417 1101 40-41 0.35988025510020805 1101 42-43 0.40348845058124994 1101 44-45 0.17624108811902062 1101 46-47 0.15511194847894672 1101 48-49 0.2982344849405223 1101 50-51 0.22784620996021943 1101 52-53 0.041793423552761055 1101 54-55 0.20882087973066632 1101 56-57 0.05240022545638823 1101 58-59 0.19086582304751687 1101 60-61 0.2207298301101943 1101 62-63 0.04024280221081966 1101 64-65 0.04255808817525519 1101 66-67 0.08253859839803823 1101 68-69 0.3186788392010911 1101 70-71 0.2674686486890998 1101 72-73 0.4079717212277707 1101 74-75 0.17826493566842672 1101 76-77 0.03842802350096619 1101 78-79 0.06813900892139202 1101 80-81 0.42697639917486185 1101 82-83 0.29061515930699855 1101 84-85 0.17213956028869148 1101 86-87 0.10466507229670441 1101 88-89 0.3289355132361287 1101 90-91 0.2482078543918469 1101 92-93 0.31171198145524315 1101 94-95 0.18510336164511898 1101 96-97 0.03179698999079861 1101 98-99 -0.03984025051308748 1101 100-101 0.5035719136635528 1102 1 0.22938190086972554 1102 2 0.20725091950768615 1102 3 0.15918193849634577 1102 4 0.05115585167268932 1102 5 0.09053918638220892 1102 6 -0.05837868387260414 1102 7 0.046371862463452374 1102 8 0.05372033646332852 1102 9 0.2417313903395808 1102 10-11 0.14450540672134338 1102 12-13 0.2072025914467659 1102 14-15 0.43856406123033054 1102 16-17 0.50333322323349 1102 18-19 0.3192608008179789 1102 20-21 0.3840838652652323 1102 22-23 0.2343586077054809 1102 24-25 0.2882940045472111 1102 26-27 0.20494797248440477 1102 28-29 0.3261925178157199 1102 30-31 0.18590495878314783 1102 32-33 0.29997571198658335 1102 34-35 0.3310932329583167 1102 36-37 0.40653725670569685 1102 38-39 0.487062953170053 1102 40-41 0.24896444786073602 1102 42-43 0.3267039389333348 1102 44-45 0.1370972561150552 1102 46-47 0.2245764529863692 1102 48-49 0.23628371368744894 1102 50-51 0.06660015548159492 1102 52-53 0.04269612638704956 1102 54-55 0.2921007946940293 1102 56-57 0.038021893570082455 1102 58-59 -0.02222580230444038 1102 60-61 0.0987270063745953 1102 62-63 0.05508567106128481 1102 64-65 0.13241657682757335 1102 66-67 0.3039513181909683 1102 68-69 0.03529911978953493 1102 70-71 0.09353911225236189 1102 72-73 -0.002376701780807622 1102 74-75 -0.13290281694636974 1102 76-77 -0.1012231477813188 1102 78-79 -0.12139410100144232 1102 80-81 -0.10386560957222812 1102 82-83 -0.18427245926149638 1102 84-85 0.01503275330075482 1102 86-87 -0.07297365994337923 1102 88-89 -0.19692844437537715 1102 90-91 0.023219822811444857 1102 92-93 0.14425857714985924 1102 94-95 0.20304889113611324 1102 96-97 0.2853256452272852 1102 98-99 0.2793524288712099 1102 100-101 0.7978013097249281 1103 1 0.22104101892466588 1103 2 0.199847935868803 1103 3 0.2576933255437268 1103 4 0.1447873618492892 1103 5 -0.021631847790956726 1103 6 0.03853947848801198 1103 7 0.11333940476908566 1103 8 0.16986341004425043 1103 9 0.1770865491810767 1103 10-11 0.25391084332503056 1103 12-13 0.22998370041131722 1103 14-15 0.2605627866505813 1103 16-17 0.2981739809270678 1103 18-19 0.1787073205739631 1103 20-21 0.21393548494346248 1103 22-23 0.11684475967707186 1103 24-25 0.2331213350641832 1103 26-27 0.2766931847941194 1103 28-29 0.036622427953844294 1103 30-31 0.2645801974662376 1103 32-33 0.4669636956655481 1103 34-35 0.26035105078160115 1103 36-37 0.28110538259249296 1103 38-39 0.23863112715419277 1103 40-41 0.39507718003064696 1103 42-43 0.3117516755522516 1103 44-45 0.34120336688324926 1103 46-47 0.36221403144516984 1103 48-49 0.42534337043269943 1103 50-51 0.3672877471671683 1103 52-53 0.40263464194253373 1103 54-55 0.23686900555281198 1103 56-57 0.041414760733225364 1103 58-59 0.46101728169757905 1103 60-61 0.39162382954469166 1103 62-63 0.20915751246829473 1103 64-65 0.16449951268975127 1103 66-67 0.3907780555072975 1103 68-69 0.3859388145316913 1103 70-71 0.3930172560153373 1103 72-73 0.4338141485001614 1103 74-75 0.5861234923035532 1103 76-77 0.426094142414037 1103 78-79 0.27471963319657533 1103 80-81 0.5359674124643732 1103 82-83 0.5397259134448618 1103 84-85 0.6604410597129338 1103 86-87 0.623450903372774 1103 88-89 0.6269230310108007 1103 90-91 0.7432391419133069 1103 92-93 0.6632540239341473 1103 94-95 0.4460878018914798 1103 96-97 0.6252446412752874 1103 98-99 0.4691515823578669 1103 100-101 0.8095841582102601 1104 1 0.029611811267656662 1104 2 0.07413516119705577 1104 3 0.07228840590732943 1104 4 -0.002258947628135388 1104 5 -0.036537487160778426 1104 6 0.04372672847011927 1104 7 0.09493649720511854 1104 8 0.1419051466297745 1104 9 0.17908868054634297 1104 10-11 0.011897498433548037 1104 12-13 -0.13925544990561178 1104 14-15 -0.10704205186668503 1104 16-17 0.11582594011300529 1104 18-19 0.06922930624291723 1104 20-21 0.12371033960503297 1104 22-23 0.0848991333499356 1104 24-25 0.029077904520448783 1104 26-27 0.10509442225843202 1104 28-29 -0.17779351434023738 1104 30-31 -0.16573787698839482 1104 32-33 -0.05307650601886138 1104 34-35 -0.020236625473565084 1104 36-37 -0.03996133391206769 1104 38-39 0.19828132080622396 1104 40-41 0.2170584749145803 1104 42-43 0.09201030241221986 1104 44-45 0.24855458883948955 1104 46-47 0.05496241900372212 1104 48-49 0.17013723241884549 1104 50-51 0.10038792928756379 1104 52-53 -0.059797062911094656 1104 54-55 -0.044934463482753983 1104 56-57 -0.036580881101976104 1104 58-59 0.08570413801334098 1104 60-61 0.1020752733856014 1104 62-63 -0.04868698534864535 1104 64-65 0.0885308548931718 1104 66-67 0.07683212697165942 1104 68-69 0.10266207901980806 1104 70-71 -0.04059628292581863 1104 72-73 0.07543064590355542 1104 74-75 0.09133658906594633 1104 76-77 -0.0017396588122942092 1104 78-79 -0.14717437909853714 1104 80-81 0.15285848220296216 1104 82-83 0.17775964945777645 1104 84-85 0.2103601394085004 1104 86-87 0.11991486500819803 1104 88-89 0.26915189828559605 1104 90-91 0.3372915989480063 1104 92-93 0.4266645264750899 1104 94-95 0.5529851146216203 1104 96-97 0.3884841574235587 1104 98-99 0.42566835095362876 1104 100-101 1.4266849845119474 1105 1 0.19734370934182976 1105 2 0.2759895048182841 1105 3 0.22227579132487563 1105 4 0.19021855636648866 1105 5 0.013647526715267588 1105 6 0.09925191558642865 1105 7 0.17432426946985657 1105 8 0.22457271033341897 1105 9 0.22120036164970003 1105 10-11 0.198908683363328 1105 12-13 0.19324391936527263 1105 14-15 0.26767412002802615 1105 16-17 0.29761258547505065 1105 18-19 0.389503042682243 1105 20-21 0.2848406061932067 1105 22-23 0.1777109237796708 1105 24-25 0.31829874381066503 1105 26-27 0.251423578763351 1105 28-29 0.3924381734908877 1105 30-31 0.3801897153082976 1105 32-33 0.4339620105062423 1105 34-35 0.5863671084428503 1105 36-37 0.4713707659954238 1105 38-39 0.5676468073086198 1105 40-41 0.39286370576143526 1105 42-43 0.7344289383553786 1105 44-45 0.7317351455630714 1105 46-47 0.5268634365800438 1105 48-49 0.5682219183157429 1105 50-51 0.4798976425160504 1105 52-53 0.5709005234987927 1105 54-55 0.5611541749833222 1105 56-57 0.6916799884632425 1105 58-59 0.6987307968309295 1105 60-61 0.6205661488376251 1105 62-63 0.8022401864619653 1105 64-65 0.5800265238865308 1105 66-67 0.3668939384256973 1105 68-69 0.2745567893049028 1105 70-71 0.29175591621638475 1105 72-73 0.4954752174282362 1105 74-75 0.3814719855843194 1105 76-77 0.42202621453729705 1105 78-79 0.41176725743329 1105 80-81 0.5107552108212481 1105 82-83 0.6305600867633032 1105 84-85 0.5139616027000642 1105 86-87 0.7232177831816067 1105 88-89 0.7501459273832332 1105 90-91 0.6749629470263834 1105 92-93 0.6740995613754315 1105 94-95 0.8183854173715943 1105 96-97 1.132870668230055 1105 98-99 1.5235680229744872 1105 100-101 2.2265861702826975 1106 1 0.18281839458803972 1106 2 -0.008570359136122363 1106 3 0.11202063166231824 1106 4 0.10938028853692572 1106 5 0.041586387797067914 1106 6 0.07152261218955402 1106 7 0.05077610551044387 1106 8 0.1304794553091284 1106 9 0.08708344329745188 1106 10-11 0.10282648482503731 1106 12-13 0.09496553774403793 1106 14-15 0.17556285416979023 1106 16-17 0.1348483897963888 1106 18-19 0.0769018569195552 1106 20-21 0.06811762476224459 1106 22-23 0.13651985724232674 1106 24-25 0.20730874138128286 1106 26-27 0.26402995710583355 1106 28-29 0.23045657376832196 1106 30-31 0.2229208453805711 1106 32-33 0.4029521093692452 1106 34-35 0.37911733979081674 1106 36-37 0.22863620511150629 1106 38-39 0.4936984438057763 1106 40-41 0.6039782373814617 1106 42-43 0.6579733006045814 1106 44-45 0.5072432302243683 1106 46-47 0.6100838003944347 1106 48-49 0.5294681189236528 1106 50-51 0.5733158621658845 1106 52-53 0.7686427712553225 1106 54-55 0.8413448539316732 1106 56-57 0.746264665531136 1106 58-59 0.6704011013942832 1106 60-61 0.5392410766514857 1106 62-63 0.7223676457512127 1106 64-65 0.5520496457026027 1106 66-67 0.6057322099642377 1106 68-69 0.6917805765129756 1106 70-71 0.5391220728357204 1106 72-73 0.681815579907397 1106 74-75 0.7400335297091942 1106 76-77 0.5745216200314154 1106 78-79 0.44799755576554645 1106 80-81 0.409963064227302 1106 82-83 0.30604193035166816 1106 84-85 0.3527222637154743 1106 86-87 0.5164228288855846 1106 88-89 0.6749865800905148 1106 90-91 0.4671058319866326 1106 92-93 0.3624390670789275 1106 94-95 0.6939744506430543 1106 96-97 0.8743668915554927 1106 98-99 1.1345533891719057 1106 100-101 1.5058287397937242 1107 1 0.027010167147331288 1107 2 0.028553502602541414 1107 3 0.04461485471878035 1107 4 -0.045018137815368675 1107 5 -0.13008255201631158 1107 6 0.014998231003104934 1107 7 0.08369487089919403 1107 8 0.0278781652421074 1107 9 0.11672437614284803 1107 10-11 -0.037202503422115285 1107 12-13 0.1331359472255329 1107 14-15 0.16921469811939716 1107 16-17 0.11705392727180453 1107 18-19 0.06234246477248462 1107 20-21 0.08016620258263885 1107 22-23 0.15420223787960907 1107 24-25 0.006964393414278902 1107 26-27 0.08497621631659058 1107 28-29 -0.041714258833550844 1107 30-31 -0.1381696348610575 1107 32-33 -0.1814937154679157 1107 34-35 -0.11248597135119809 1107 36-37 -0.10769408085485566 1107 38-39 -0.11763983193366556 1107 40-41 -0.3877866697346093 1107 42-43 -0.23751056455505193 1107 44-45 -0.04166048288200841 1107 46-47 -0.19157537164000615 1107 48-49 -0.13089405411458443 1107 50-51 -0.16892955665576892 1107 52-53 -0.20655382217551477 1107 54-55 -0.1730236839173429 1107 56-57 -0.19664694282143813 1107 58-59 -0.12348976055594107 1107 60-61 -0.05089796790668544 1107 62-63 -0.012472266203737092 1107 64-65 -0.3855004873102459 1107 66-67 -0.39352777950276874 1107 68-69 -0.5126527347640781 1107 70-71 -0.45111419938372066 1107 72-73 -0.40176599905181476 1107 74-75 -0.4372165479378971 1107 76-77 -0.3442613237160792 1107 78-79 -0.1840664164345469 1107 80-81 -0.13284546479920678 1107 82-83 -0.24712854664903716 1107 84-85 -0.3211820120509792 1107 86-87 -0.2806738451181694 1107 88-89 -0.3856524217054975 1107 90-91 -0.07721692783853484 1107 92-93 0.03263698838753726 1107 94-95 -0.019807570341917824 1107 96-97 0.173433776084142 1107 98-99 0.4285747882599438 1107 100-101 1.2803420388272961 1108 1 0.2641335824026996 1108 2 0.14290506406307202 1108 3 0.25318245465035005 1108 4 0.202078476961006 1108 5 0.09884200748645355 1108 6 0.18285476290707692 1108 7 0.131093703842879 1108 8 0.19998951077820948 1108 9 0.21216544360022027 1108 10-11 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4 3.0 5 36.0 6 93.0 7 237.0 8 557.0 9 996.0 10 1769.0 11 2677.0 12 3730.0 13 4911.0 14 5668.0 15 7368.0 16 8734.0 17 11415.0 18 11012.0 19 7911.0 20 7138.0 21 7293.0 22 8004.0 23 8650.0 24 9691.0 25 11610.0 26 13085.0 27 15418.0 28 18763.0 29 22725.0 30 27502.0 31 32702.0 32 40232.0 33 50480.0 34 66753.0 35 93262.0 36 137975.0 37 210432.0 38 171887.0 39 16141.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.63964664187978 20.400553741330874 14.926847754002734 23.03295186278661 2 15.993677100030284 22.00081784917241 38.349024849884174 23.656480200913137 3 18.637220238680793 25.244534973661015 31.69242698670802 24.42581780095017 4 13.270856600563622 18.147957402403012 36.699920722639185 31.88126527439418 5 13.074839329573585 37.553275438599876 34.87305541031493 14.498829821511606 6 30.47162159164451 37.007620364824604 17.43502050004485 15.085737543486038 7 26.185929522233348 33.99233845067733 21.83724801469042 17.9844840123989 8 25.172105170774238 34.62144577987898 20.9739734931997 19.232475556147083 9 25.36296879249585 16.826089388599875 21.526696795921556 36.28424502298272 10-11 24.09723146481802 26.758809720831817 28.25567623974554 20.888282574604624 12-13 25.085642696356135 24.714765456674247 28.341656491774252 21.85793535519537 14-15 22.31455173571127 26.461519617771245 26.849853307949147 24.37407533856834 16-17 21.136943851564514 29.25381037208212 27.80349156717375 21.805754209179614 18-19 21.043992184139512 27.608437348702726 30.269870937999706 21.07769952915806 20-21 21.832522235274375 26.89937754733977 30.25959960110564 21.008500616280212 22-23 21.615377493330865 26.9318311141459 30.123323553863273 21.329467838659962 24-25 21.67032755066366 27.081604359305118 30.23168452904797 21.016383560983254 26-27 21.381919361916566 27.163055586364536 30.275496573773573 21.179528477945325 28-29 21.163969114620404 27.509581758877232 30.141985560332774 21.184463566169594 30-31 21.43849832090164 27.09120561384017 29.944563714115418 21.52573235114277 32-33 21.03839840442256 27.15360519102758 30.05074908425968 21.757247320290183 34-35 21.70465176840501 27.1741688448221 29.728783480097608 21.392395906675283 36-37 21.795716432624097 26.87266206619813 29.920343858335922 21.411277642841846 38-39 21.36979470104135 27.029502320754062 29.898228278860884 21.702474699343707 40-41 21.60083256333442 27.09264485463899 29.8086030258546 21.497919556171983 42-43 21.522067695679052 27.097272631697734 29.43714572038423 21.943513952238984 44-45 21.46279666174461 27.170729213785492 29.58139673948163 21.785077384988266 46-47 21.880849988571274 27.26710716286947 28.908792981894393 21.943249866664864 48-49 21.637488378193826 27.26861514364745 28.82540671499281 22.268489763165917 50-51 21.417714535918815 27.69740737954567 28.740647296757732 22.144230787777786 52-53 21.649518838499866 27.63447735772993 28.636824816321493 22.079178987448717 54-55 21.2363024730293 27.49501864271757 28.638078594533912 22.63060028971921 56-57 21.37317742641712 27.753366420474162 28.218907507103193 22.65454864600553 58-59 21.351663623834586 27.520463166300658 28.48621273977372 22.641660470091036 60-61 21.18186763267692 27.691297227542865 28.593266862260595 22.53356827751962 62-63 20.95214580974444 27.737501181453972 28.593886530267405 22.716466478534187 64-65 20.804883659137257 27.798789694318003 28.310582434895075 23.08574421164966 66-67 20.83808950623089 27.70496633666422 28.12910363079849 23.3278405263064 68-69 21.024860564992675 27.38192027297724 28.1501027618885 23.44311640014158 70-71 21.118767206322524 27.239769516653332 27.916328507726902 23.72513476929724 72-73 20.998518612819787 27.2478314459149 27.960507915198303 23.793142026067013 74-75 20.81385339836959 27.589615737938917 27.65804365595079 23.938487207740696 76-77 20.81333710338465 27.093415888450227 27.953257797202763 24.139989210962355 78-79 20.888093085784483 27.16677676563985 27.81254587457216 24.132584274003506 80-81 20.968917166614347 26.94586580521836 27.682610500854267 24.402606527313033 82-83 20.795026515189875 26.743764939960467 27.856790487805235 24.604418057044427 84-85 20.633186930615913 26.759870610088477 27.746304729830086 24.860637729465523 86-87 20.599448727293794 26.603880148872594 27.72781030812379 25.068860815709826 88-89 20.56086253191888 26.422177456622503 27.522117598897104 25.494842412561507 90-91 20.556452151620793 26.24445866520027 27.59553266035381 25.603556522825123 92-93 20.585478106054612 26.13096053283851 27.639165096223028 25.644396264883852 94-95 20.309000101749465 25.81075274832053 27.779821875748954 26.10042527418105 96-97 20.224522961041217 25.530903251628708 27.93902774228082 26.30554604504926 98-99 20.095846522115686 25.63798022116648 27.630667800853725 26.635505455864113 100-101 20.219809047460373 25.069091179138443 28.04863170290528 26.66246807049591 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1045.0 1 722.5 2 483.5 3 554.0 4 478.5 5 486.0 6 733.0 7 1190.0 8 1644.5 9 1877.5 10 1689.0 11 1416.5 12 1390.0 13 1449.5 14 1542.5 15 1647.5 16 1801.0 17 1932.0 18 2040.0 19 2221.0 20 2513.0 21 2897.5 22 3348.5 23 3915.5 24 4655.5 25 5586.0 26 6973.0 27 8656.5 28 10008.0 29 11436.5 30 13089.5 31 15012.0 32 17606.5 33 19218.5 34 19908.5 35 21290.0 36 23455.5 37 25480.5 38 26763.0 39 27900.5 40 29030.0 41 30102.5 42 31139.5 43 32454.0 44 33957.0 45 35988.5 46 37694.5 47 39028.5 48 41451.0 49 41917.5 50 40106.5 51 38619.0 52 37323.0 53 36509.5 54 35085.5 55 32068.5 56 28944.5 57 25752.5 58 23245.0 59 20596.0 60 16752.5 61 13455.0 62 10876.0 63 8135.0 64 5516.0 65 3887.0 66 2860.5 67 2178.0 68 1644.0 69 1288.0 70 991.5 71 659.5 72 513.5 73 445.5 74 332.0 75 223.5 76 150.0 77 111.0 78 84.5 79 60.5 80 45.5 81 29.5 82 20.0 83 14.0 84 7.5 85 5.0 86 6.5 87 9.0 88 8.0 89 5.0 90 2.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23609608184664171 2 0.0 3 0.0 4 0.0 5 0.026232897982960187 6 0.003761334637262674 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0024111119469632527 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.3755567257485536E-4 26-27 0.0010608892566638312 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005400890761197686 36-37 0.015431116460564817 38-39 0.012923560035723035 40-41 0.006558224495740047 42-43 0.007619113752403879 44-45 0.003568445681505614 46-47 4.8222238939265053E-4 48-49 0.0021217785133276624 50-51 0.0 52-53 0.0 54-55 0.0 56-57 7.715558230282408E-4 58-59 2.8933343363559034E-4 60-61 0.0023146674690847227 62-63 7.715558230282408E-4 64-65 0.0019771117965098673 66-67 0.005352668522258421 68-69 2.8933343363559034E-4 70-71 1.4466671681779517E-4 72-73 0.0 74-75 8.197780619675058E-4 76-77 0.06066357658559544 78-79 0.13830138127781216 80-81 7.233335840889758E-4 82-83 7.233335840889758E-4 84-85 0.0 86-87 0.10425648058669104 88-89 0.3455123419998341 90-91 0.3806663541865583 92-93 3.857779115141204E-4 94-95 5.304446283319156E-4 96-97 9.64444778785301E-5 98-99 0.0 100-101 0.0011091114956030962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1036866.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.88467442508663 #Duplication Level Percentage of deduplicated Percentage of total 1 81.33465264176351 51.1470315085481 2 11.189906349855129 14.073472353156985 3 3.1872405689313488 6.012857564750273 4 1.3931566083243203 3.5043279895053123 5 0.7303029177757534 2.2962430608009528 6 0.4746803789600945 1.7910072652129374 7 0.32413982803807245 1.4268399288064426 8 0.2243230434588548 1.1285185243163713 9 0.17362926049445482 0.9826757565176113 >10 0.8901064327549156 9.810688212906818 >50 0.04838514900241465 2.058041956296572 >100 0.026713369394575814 3.2632754584895993 >500 0.0016887757617380617 0.7570960087511756 >1k 0.001074675484742403 1.7479244119408357 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4798 0.46274060486118745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4232 0.4081530303819394 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2709 0.2612680905729381 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2372 0.22876630152787342 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1478 0.14254493830446752 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1461 0.1409053821805325 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1055 0.10174892416184927 RNA PCR Primer, Index 27 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.9288895575706022E-4 2 0.0 0.0 0.0 0.0 1.9288895575706022E-4 3 0.0 0.0 0.0 0.0 1.9288895575706022E-4 4 0.0 0.0 0.0 0.0 1.9288895575706022E-4 5 0.0 0.0 0.0 0.0 1.9288895575706022E-4 6 0.0 0.0 0.0 0.0 1.9288895575706022E-4 7 0.0 0.0 0.0 0.0 1.9288895575706022E-4 8 0.0 0.0 0.0 0.0 1.9288895575706022E-4 9 0.0 0.0 0.0 0.0 2.8933343363559034E-4 10-11 0.0 0.0 0.0 0.0 2.8933343363559034E-4 12-13 9.644447787853011E-5 0.0 0.0 0.0 2.8933343363559034E-4 14-15 9.644447787853011E-5 0.0 0.0 0.0 2.8933343363559034E-4 16-17 9.644447787853011E-5 0.0 0.0 4.8222238939265055E-5 3.375556725748554E-4 18-19 9.644447787853011E-5 0.0 0.0 9.644447787853011E-5 3.8577791151412044E-4 20-21 9.644447787853011E-5 9.644447787853011E-5 0.0 5.304446283319156E-4 3.8577791151412044E-4 22-23 9.644447787853011E-5 9.644447787853011E-5 0.0 0.0010126670177245663 4.3400015045338546E-4 24-25 9.644447787853011E-5 9.644447787853011E-5 0.0 0.003713112398323409 4.8222238939265053E-4 26-27 9.644447787853011E-5 9.644447787853011E-5 0.0 0.007812002708160938 4.8222238939265053E-4 28-29 9.644447787853011E-5 9.644447787853011E-5 0.0 0.021796452000547806 5.786668672711807E-4 30-31 9.644447787853011E-5 9.644447787853011E-5 0.0 0.05830068687757145 5.786668672711807E-4 32-33 9.644447787853011E-5 9.644447787853011E-5 0.0 0.13222537917146476 6.751113451497107E-4 34-35 9.644447787853011E-5 9.644447787853011E-5 0.0 0.2582300895197644 6.751113451497107E-4 36-37 9.644447787853011E-5 9.644447787853011E-5 0.0 0.4563270470822652 6.751113451497107E-4 38-39 9.644447787853011E-5 9.644447787853011E-5 0.0 0.7120978024161271 7.715558230282409E-4 40-41 9.644447787853011E-5 9.644447787853011E-5 0.0 0.9912563436355325 7.715558230282409E-4 42-43 9.644447787853011E-5 9.644447787853011E-5 0.0 1.2791913323418842 7.715558230282409E-4 44-45 9.644447787853011E-5 9.644447787853011E-5 0.0 1.6243661186691434 7.715558230282409E-4 46-47 9.644447787853011E-5 9.644447787853011E-5 0.0 2.0314100375554798 7.715558230282409E-4 48-49 9.644447787853011E-5 9.644447787853011E-5 0.0 2.4325226210522866 9.162225398460361E-4 50-51 9.644447787853011E-5 9.644447787853011E-5 0.0 2.8658476601605223 0.0010608892566638312 52-53 9.644447787853011E-5 9.644447787853011E-5 0.0 3.2801249148877485 0.0012055559734816266 54-55 9.644447787853011E-5 9.644447787853011E-5 0.0 3.7406472967577296 0.0014948894071172168 56-57 9.644447787853011E-5 9.644447787853011E-5 0.0 4.2857514857271815 0.0015431116460564818 58-59 9.644447787853011E-5 9.644447787853011E-5 0.0 4.875509467954393 0.0015431116460564818 60-61 9.644447787853011E-5 9.644447787853011E-5 0.0 5.495502793996524 0.0015431116460564818 62-63 9.644447787853011E-5 9.644447787853011E-5 0.0 6.106285672401255 0.001639556123935012 64-65 9.644447787853011E-5 9.644447787853011E-5 0.0 6.759070120922086 0.001639556123935012 66-67 9.644447787853011E-5 9.644447787853011E-5 0.0 7.485489928303175 0.001639556123935012 68-69 9.644447787853011E-5 9.644447787853011E-5 0.0 8.291765763367687 0.001639556123935012 70-71 9.644447787853011E-5 9.644447787853011E-5 0.0 9.148144504690094 0.001687778362874277 72-73 9.644447787853011E-5 9.644447787853011E-5 0.0 9.999893911074334 0.001736000601813542 74-75 9.644447787853011E-5 9.644447787853011E-5 0.0 10.867026211680198 0.001784222840752807 76-77 9.644447787853011E-5 9.644447787853011E-5 0.0 11.801090979933761 0.001832445079692072 78-79 9.644447787853011E-5 9.644447787853011E-5 0.0 12.819641110808918 0.001832445079692072 80-81 9.644447787853011E-5 9.644447787853011E-5 0.0 13.880867923145324 0.001832445079692072 82-83 9.644447787853011E-5 9.644447787853011E-5 0.0 14.962492742553039 0.002025334035449132 84-85 9.644447787853011E-5 9.644447787853011E-5 0.0 16.058246677969958 0.002025334035449132 86-87 9.644447787853011E-5 9.644447787853011E-5 0.0 17.163066394307464 0.002025334035449132 88-89 9.644447787853011E-5 9.644447787853011E-5 0.0 18.33370946679706 0.002025334035449132 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4815 0.0 26.881721 1 GTATCAA 6700 0.0 26.776327 1 TATCAAC 7775 0.0 23.011806 2 ATCAACG 7890 0.0 22.49595 3 CAACGCA 7980 0.0 22.242237 5 TCAACGC 7985 0.0 22.228312 4 AACGCAG 8135 0.0 21.813185 6 CCGTTAT 155 3.9399783E-5 21.427494 9 GAGTACT 4690 0.0 21.094011 12-13 GTACTAG 120 0.0047132703 19.82774 1 TACATGG 3385 0.0 19.768341 2 ACGCAGA 8945 0.0 19.678799 7 GTACATG 3460 0.0 19.667284 1 CGCAGAG 8945 0.0 19.51967 8 AGAGTAC 6710 0.0 19.481619 10-11 GTATAAT 470 0.0 19.237125 1 AGTACTT 4915 0.0 19.114708 12-13 ACCGCTA 75 7.775005E-4 18.981382 60-61 GTACTTT 5250 0.0 18.889174 14-15 GCAGAGT 9390 0.0 18.190388 9 >>END_MODULE