FastQCFastQC Report
Thu 26 May 2016
SRR938827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445156
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36780.8262272102364114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29050.6525802190692701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.4407443682664055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12860.28888749112670614No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.20352415782332486No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7900.1774658771307137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.14421910521255468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.13500885082982148No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.11995794732633054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.038.7385061
GTATCAA28050.034.48821
CGGTATC753.1095993E-431.6070772
GCGGTAT953.5257723E-529.950281
TATCAAC35050.026.78262
ATCAACG35150.026.7064063
CAACGCA35600.026.3688265
TCAACGC35600.026.3688264
AACGCAG36250.026.1428856
GTACATG16250.023.6376841
ACGCAGA41200.023.2319497
CGCAGAG41400.022.8908128
TAAGGTG1252.2817985E-422.7570975
TAAGGCG1503.072172E-522.1249525
TACATGG16250.021.8818232
GTATAGC2005.45715E-721.3395771
GCAGAGT43250.021.1447569
GAGTACT27350.021.13654712-13
AGAGTAC35100.020.92454710-11
AGTACTT27700.020.86947612-13
Produced by FastQC (version 0.11.5)