FastQCFastQC Report
Thu 26 May 2016
SRR938827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445156
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.7660235962224479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33460.751646613771352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.494208771756418No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT14900.334714122689574RNA PCR Primer, Index 26 (100% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13570.30483695603339056No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10860.24395942096703177No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.19094429817861602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.18690077186424536No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT8280.18600221046105186RNA PCR Primer, Index 26 (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.1617410525748277No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT6390.14354518416015957No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG6020.1352334911806198RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG600.00396397431.641175
GCGTGCC1102.7456772E-630.2029329
ATAGCGT908.981039E-426.3676416
AGCGTGC1151.2870487E-424.7626558
GTATCAA47100.023.8536011
GGTATCA35300.023.7356341
AGTACTT33700.022.04084412-13
GAGTACT32750.021.81066912-13
CTATAGC1353.81158E-421.0941144
TAAGGTG1404.8717053E-420.3407545
GTAAGGT1404.8717053E-420.3407543
TATCAAC55550.020.2492082
GTACTTT35050.020.24402814-15
AGAGTAC44400.020.09641610-11
ATCAACG56400.019.8598823
ATATAAC1200.004697930519.8358861
CAACGCA56850.019.702685
TCAACGC56900.019.6853664
AACGCAG58000.019.3120256
GTATACA1250.00605356318.9847014
Produced by FastQC (version 0.11.5)