##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938827_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 445156 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15236905713952 31.0 31.0 33.0 30.0 34.0 2 31.400540484684022 31.0 31.0 34.0 30.0 34.0 3 31.397590956878037 31.0 31.0 34.0 28.0 34.0 4 35.24370333096712 37.0 35.0 37.0 33.0 37.0 5 35.082530618479815 35.0 35.0 37.0 32.0 37.0 6 35.17753551563946 37.0 35.0 37.0 32.0 37.0 7 35.10625039312061 37.0 35.0 37.0 32.0 37.0 8 35.09004259181051 37.0 35.0 37.0 32.0 37.0 9 36.592452982774574 38.0 35.0 39.0 32.0 39.0 10-11 36.46328815067078 38.0 35.0 39.0 32.0 39.0 12-13 36.36898862421263 38.0 35.0 39.0 32.0 39.0 14-15 37.27027828446657 39.0 36.0 40.0 32.0 41.0 16-17 37.122157176360645 39.0 36.0 40.0 31.0 41.0 18-19 37.16717397945889 38.5 36.0 40.0 31.5 41.0 20-21 37.25427041306868 39.0 36.0 40.0 31.0 41.0 22-23 37.15967323814573 39.0 36.0 40.0 31.0 41.0 24-25 37.00754903898858 39.0 36.0 40.0 31.0 41.0 26-27 36.68718157230275 39.0 35.0 40.0 30.0 41.0 28-29 36.40016196569293 38.0 35.0 40.0 30.0 41.0 30-31 35.985292796233225 38.0 35.0 40.0 29.5 41.0 32-33 35.54495615020352 38.0 34.0 40.0 28.5 41.0 34-35 35.32335069054444 38.0 34.0 40.0 27.0 41.0 36-37 35.075772313526045 38.0 34.0 40.0 26.5 41.0 38-39 34.8696827628966 38.0 33.5 40.0 25.5 41.0 40-41 34.537092614723825 38.0 33.0 40.0 25.0 41.0 42-43 34.16064817726819 37.5 33.0 40.0 24.0 41.0 44-45 33.66721104511677 37.0 33.0 40.0 22.5 41.0 46-47 33.07239259944828 37.0 32.0 40.0 19.0 41.0 48-49 32.517958872844574 37.0 31.0 40.0 14.0 41.0 50-51 32.13005665429647 36.5 31.0 39.0 9.0 40.5 52-53 31.787921537618274 36.0 30.5 39.0 8.0 40.0 54-55 31.380304881884104 36.0 30.0 39.0 6.5 40.0 56-57 30.926444886736334 35.0 29.0 39.0 2.0 40.0 58-59 30.443821941072343 35.0 28.5 38.5 2.0 40.0 60-61 29.885207657540278 34.5 28.0 38.0 2.0 40.0 62-63 29.310840469408475 34.0 26.5 38.0 2.0 40.0 64-65 28.77249548472895 34.0 26.0 37.5 2.0 39.5 66-67 28.21113946571539 33.5 26.0 37.0 2.0 39.0 68-69 27.6041926875073 33.0 24.5 36.0 2.0 39.0 70-71 26.96939499860723 32.0 23.0 36.0 2.0 38.5 72-73 26.491064930046996 32.0 22.0 35.0 2.0 38.0 74-75 25.971780679132706 31.5 20.5 35.0 2.0 37.5 76-77 24.36111947272417 29.5 19.5 33.5 2.0 35.5 78-79 25.327108923613295 31.0 20.0 34.5 2.0 36.0 80-81 25.325903728131262 31.0 19.5 35.0 2.0 36.0 82-83 24.857848709216544 31.0 18.0 34.0 2.0 36.0 84-85 24.26664697319591 31.0 12.5 34.0 2.0 35.0 86-87 23.514489302626494 30.5 6.5 34.0 2.0 35.0 88-89 22.744864721580747 29.5 2.0 34.0 2.0 35.0 90-91 22.040612729020836 29.0 2.0 34.0 2.0 35.0 92-93 21.438793366819723 29.0 2.0 34.0 2.0 35.0 94-95 20.682932500067395 29.0 2.0 34.0 2.0 35.0 96-97 19.689986207082462 27.0 2.0 33.0 2.0 35.0 98-99 18.44815413023749 25.0 2.0 33.0 2.0 34.0 100-101 15.596670605360817 10.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11820121943746997 1101 2 0.15123210859711023 1101 3 0.13746649993185045 1101 4 0.24984556980478345 1101 5 0.1693313660777207 1101 6 0.2575369201458386 1101 7 0.3615374118545347 1101 8 0.09992914430115007 1101 9 0.34597480256296365 1101 10-11 0.1884809200444053 1101 12-13 0.11601447963703748 1101 14-15 0.22123255759624527 1101 16-17 0.41475006710252416 1101 18-19 0.2008140668334235 1101 20-21 0.3047564613797107 1101 22-23 0.3483502036855981 1101 24-25 0.2765349693815793 1101 26-27 0.27771312655335834 1101 28-29 0.5182522007016814 1101 30-31 0.2318262625548826 1101 32-33 0.30747146033387907 1101 34-35 0.1435183210847697 1101 36-37 -0.015081944658419388 1101 38-39 0.06096435524265331 1101 40-41 0.28257910191473457 1101 42-43 0.37083905845383924 1101 44-45 0.5529088106100275 1101 46-47 0.525828853420073 1101 48-49 0.3448974473121851 1101 50-51 0.45933622335448376 1101 52-53 0.3250857590970355 1101 54-55 0.3269379615852053 1101 56-57 0.23201799615293695 1101 58-59 0.17400567447973003 1101 60-61 0.7665336423037807 1101 62-63 0.4099134819263348 1101 64-65 0.34303023939316546 1101 66-67 0.47297453082962093 1101 68-69 0.6927598455143134 1101 70-71 0.3399158575421417 1101 72-73 0.36756073802177625 1101 74-75 0.13692749555483275 1101 76-77 0.2597422224295123 1101 78-79 0.4188414463336585 1101 80-81 0.4919308077072735 1101 82-83 0.7688471610751328 1101 84-85 1.1532305015330273 1101 86-87 0.8982792977212135 1101 88-89 0.6072904352938622 1101 90-91 1.0888925508122895 1101 92-93 0.9324203597035243 1101 94-95 0.8931480600241102 1101 96-97 -0.0035849055968739663 1101 98-99 0.11975160546795749 1101 100-101 0.1122992254984787 1102 1 0.3103278045569837 1102 2 0.28309609692614046 1102 3 0.1390196365046279 1102 4 0.20532531394123765 1102 5 0.28388641040495344 1102 6 0.27088222370323933 1102 7 -0.012701169673910329 1102 8 0.17332158061581993 1102 9 0.4857032281044269 1102 10-11 0.5926824686921464 1102 12-13 0.4930047164230231 1102 14-15 0.3504099787610926 1102 16-17 0.23106697878470328 1102 18-19 0.27587186057149893 1102 20-21 0.48294970568450424 1102 22-23 0.44473444787816874 1102 24-25 0.2781891047199281 1102 26-27 0.11346220073134106 1102 28-29 0.43923525535323193 1102 30-31 0.5700509286978459 1102 32-33 0.8123261343323165 1102 34-35 0.8256505172468209 1102 36-37 0.781775871523827 1102 38-39 0.964539711543857 1102 40-41 0.8499565006354217 1102 42-43 1.0957947312178362 1102 44-45 1.2507160151886367 1102 46-47 1.3589093157699779 1102 48-49 1.1438829708224532 1102 50-51 1.536600279016028 1102 52-53 1.5998674895425609 1102 54-55 1.6090634244962132 1102 56-57 1.389674824791694 1102 58-59 1.3929036657265073 1102 60-61 1.0771048464335102 1102 62-63 1.273387839461968 1102 64-65 1.4669198140761424 1102 66-67 1.6295248618811335 1102 68-69 1.4418385123306088 1102 70-71 1.561058265801595 1102 72-73 2.0273542516566074 1102 74-75 1.9342588837267556 1102 76-77 1.984024570089705 1102 78-79 1.689684224921919 1102 80-81 1.584544431318541 1102 82-83 1.7515359726563275 1102 84-85 2.155454718792594 1102 86-87 1.8530273619115398 1102 88-89 1.6849248197625002 1102 90-91 1.5122434654122223 1102 92-93 1.9812296964782945 1102 94-95 1.8709238427645403 1102 96-97 2.3853276743604823 1102 98-99 2.2734515269133624 1102 100-101 2.563952238628321 1103 1 0.14390050750078487 1103 2 0.0502102806906386 1103 3 -0.06937911461044877 1103 4 0.15752870377174588 1103 5 0.11044751656017127 1103 6 0.08310791505024895 1103 7 0.13191524424223644 1103 8 0.14109588926881145 1103 9 -0.0879542206020858 1103 10-11 -0.06787952952373644 1103 12-13 0.08007206700286673 1103 14-15 0.20519365405101553 1103 16-17 0.22698580126463952 1103 18-19 0.03201369821645983 1103 20-21 0.16242785019832695 1103 22-23 -0.005693741238694372 1103 24-25 0.18891390400627728 1103 26-27 0.2882860187509664 1103 28-29 0.46789939451469564 1103 30-31 0.2770134621501299 1103 32-33 0.23099473379708257 1103 34-35 0.4613284833485167 1103 36-37 0.39965053700107944 1103 38-39 0.5696489889747198 1103 40-41 0.5071598904659211 1103 42-43 0.12439196582978695 1103 44-45 0.21017408833761664 1103 46-47 0.3883428572507981 1103 48-49 0.49587003594288603 1103 50-51 0.5834892174638355 1103 52-53 0.5560405493106231 1103 54-55 0.3973930409101314 1103 56-57 0.7382542181904448 1103 58-59 0.1422011679566566 1103 60-61 0.2877426698054997 1103 62-63 0.2739498376778471 1103 64-65 0.7660678961457208 1103 66-67 0.6740989029159259 1103 68-69 0.5654424373975111 1103 70-71 0.19059662440998082 1103 72-73 0.2583466691409946 1103 74-75 0.6869618275269325 1103 76-77 0.4186766664322583 1103 78-79 0.7902930563224544 1103 80-81 0.3838709255201458 1103 82-83 0.9990136711398243 1103 84-85 1.1548793396668167 1103 86-87 1.3045349444271501 1103 88-89 1.5467780757839087 1103 90-91 1.2997947589001768 1103 92-93 1.4437386170848967 1103 94-95 1.670100916805577 1103 96-97 1.2154124201231937 1103 98-99 1.4073654430596605 1103 100-101 1.4338407310458052 1104 1 -0.05544636240404088 1104 2 0.004334764063784746 1104 3 0.26489741820720525 1104 4 0.20183538105533216 1104 5 -0.027946864056367815 1104 6 -0.08081446768967027 1104 7 -0.052037093375787435 1104 8 0.10032028543155036 1104 9 -0.08659866757436419 1104 10-11 0.1195375257979947 1104 12-13 0.014766167029414135 1104 14-15 -0.25636030264712417 1104 16-17 0.14834002717750394 1104 18-19 -0.03608151279480154 1104 20-21 0.11753819031891055 1104 22-23 -0.11966446887680604 1104 24-25 -0.24855291812241376 1104 26-27 -0.038684272835126876 1104 28-29 -0.16343046936123784 1104 30-31 -0.30077916667480764 1104 32-33 0.03911525478444844 1104 34-35 0.15012479017747182 1104 36-37 -0.34661221278089016 1104 38-39 0.04815209821523325 1104 40-41 0.024900572223778283 1104 42-43 0.17703339835547638 1104 44-45 0.12707536995137758 1104 46-47 0.4282558364340545 1104 48-49 0.7075377453426483 1104 50-51 0.3775281019290091 1104 52-53 0.21007118670492275 1104 54-55 0.24744091183872996 1104 56-57 0.4514386302785667 1104 58-59 0.36025324773521916 1104 60-61 0.40543405872598726 1104 62-63 -0.05552538319198419 1104 64-65 0.7499481903860428 1104 66-67 0.5002113095439142 1104 68-69 0.7655144807752094 1104 70-71 1.0125712765163009 1104 72-73 0.6476344824348956 1104 74-75 0.6443424819841432 1104 76-77 0.9074862444097356 1104 78-79 1.1569819995598536 1104 80-81 1.0072878670891754 1104 82-83 0.75687919648772 1104 84-85 1.1978826005775964 1104 86-87 1.473552500260766 1104 88-89 1.3193413981211464 1104 90-91 1.2115558403091953 1104 92-93 1.235530885692942 1104 94-95 1.6605764978922934 1104 96-97 1.7170950121680875 1104 98-99 1.2915770420434676 1104 100-101 1.7663730557718509 1105 1 0.10687422679234615 1105 2 0.07361058539450482 1105 3 0.22072277074472169 1105 4 0.19049385326708546 1105 5 0.31168061501760747 1105 6 -0.10506100515542016 1105 7 0.07176955770633242 1105 8 -0.011184628727761492 1105 9 0.010055682273559796 1105 10-11 0.17953048761585677 1105 12-13 0.0389775135844701 1105 14-15 0.17980087172384174 1105 16-17 -0.15491764569784294 1105 18-19 0.034275172221413186 1105 20-21 0.08101002502216659 1105 22-23 -0.05980724342875021 1105 24-25 -0.0743478556585444 1105 26-27 0.03406539410804044 1105 28-29 0.21742567522313294 1105 30-31 -0.179694783898249 1105 32-33 -0.09078445112007927 1105 34-35 0.19059433747153776 1105 36-37 0.042154345799410464 1105 38-39 0.0839010362038195 1105 40-41 -0.011841252173574901 1105 42-43 0.28675865788844135 1105 44-45 0.38474321802718947 1105 46-47 0.2711032838362115 1105 48-49 0.04473453794250304 1105 50-51 0.3777593564849724 1105 52-53 0.0945570223633716 1105 54-55 0.425728119942697 1105 56-57 0.19707872780766067 1105 58-59 0.1955648129766523 1105 60-61 -0.05500633038493774 1105 62-63 -0.052111864855007184 1105 64-65 0.1533893587242794 1105 66-67 0.3236585106096932 1105 68-69 0.35174980742226936 1105 70-71 -0.07395060468924086 1105 72-73 -0.10683853367751439 1105 74-75 -0.0337009033814617 1105 76-77 0.19651422118373674 1105 78-79 0.04220935797928149 1105 80-81 -0.013610506588019433 1105 82-83 0.032647740840832995 1105 84-85 0.18755406828993415 1105 86-87 0.4937370011782427 1105 88-89 0.8949189061019425 1105 90-91 0.9993043761159477 1105 92-93 1.4723355510022422 1105 94-95 1.1318582011323706 1105 96-97 1.0572377867200302 1105 98-99 1.376987895925783 1105 100-101 1.9187245626969194 1106 1 0.004294776162069525 1106 2 -0.057391012495266125 1106 3 0.042966410889800244 1106 4 0.05715375637916509 1106 5 0.01554852279654284 1106 6 -0.04600502828677833 1106 7 -0.03581154154037591 1106 8 0.10203727504376303 1106 9 0.11209743031424324 1106 10-11 0.00530216692192198 1106 12-13 0.019389414058771592 1106 14-15 -0.1306640381095221 1106 16-17 0.07633251282296527 1106 18-19 -0.0606470891730595 1106 20-21 -0.2188276496677588 1106 22-23 -0.13146372249132554 1106 24-25 0.20526763626322975 1106 26-27 0.05442453885656562 1106 28-29 0.1649261190814002 1106 30-31 0.18063189578825956 1106 32-33 0.1573754393103357 1106 34-35 -0.0834691215793697 1106 36-37 0.010051979400742539 1106 38-39 0.10755741381307615 1106 40-41 0.2490535660981692 1106 42-43 0.282350511186948 1106 44-45 0.19214170130008057 1106 46-47 0.15542113708398375 1106 48-49 -0.17032874356509353 1106 50-51 0.12517102868930152 1106 52-53 -0.40461866942144553 1106 54-55 -0.5219143585618831 1106 56-57 -0.7864401805546279 1106 58-59 -0.7008589575359423 1106 60-61 -0.36384798711763366 1106 62-63 -0.7529564637339448 1106 64-65 -0.6562309522301426 1106 66-67 -0.27999634519977334 1106 68-69 -0.4099946823943519 1106 70-71 -0.8057232478018257 1106 72-73 -0.9369727056916162 1106 74-75 -0.8386340710077107 1106 76-77 -0.6439022446899045 1106 78-79 -0.5081368514710967 1106 80-81 -0.8868420484248354 1106 82-83 -0.3121185360759995 1106 84-85 -0.194199552325383 1106 86-87 -0.5524876671962211 1106 88-89 0.006167542822971939 1106 90-91 0.280621747460458 1106 92-93 0.012632809226307273 1106 94-95 -0.21991850011296776 1106 96-97 0.1465014892589025 1106 98-99 0.4451112631614329 1106 100-101 0.5385916588493025 1107 1 -0.03734349633676004 1107 2 0.07395508401651085 1107 3 -0.21048880440897833 1107 4 0.029570596960105888 1107 5 0.034656023115978485 1107 6 -0.05152591929576289 1107 7 0.0236245502863639 1107 8 0.05167311984124012 1107 9 -0.3020971691150365 1107 10-11 -0.13573610131778935 1107 12-13 -0.22721127166038713 1107 14-15 -0.32226876999759213 1107 16-17 -0.25064851277438294 1107 18-19 -0.059547702487087406 1107 20-21 0.05991345940843473 1107 22-23 0.04441418968551858 1107 24-25 0.09105653272389702 1107 26-27 -0.013221016746314262 1107 28-29 0.31541698325790435 1107 30-31 -0.0781392401204144 1107 32-33 0.18708283741076315 1107 34-35 0.08904674366191045 1107 36-37 0.36037567291409545 1107 38-39 0.2543907542449446 1107 40-41 -0.042968027666468345 1107 42-43 0.09688956736480492 1107 44-45 0.04126219195129721 1107 46-47 0.13526482719003496 1107 48-49 0.20719478810149639 1107 50-51 -0.45104867828288775 1107 52-53 -0.32387630390332234 1107 54-55 -0.5735855328026958 1107 56-57 -0.465275522775336 1107 58-59 -0.5366643581066626 1107 60-61 -0.7220510622060132 1107 62-63 -0.3339011376979144 1107 64-65 -0.2999098780787719 1107 66-67 -0.11301734268689145 1107 68-69 -0.31217588687495024 1107 70-71 -0.7858384571378316 1107 72-73 -0.5104213193463707 1107 74-75 -0.4803910666208111 1107 76-77 -0.575766889691824 1107 78-79 -0.5761524185736135 1107 80-81 -0.807446631243522 1107 82-83 -0.6263860730239124 1107 84-85 -0.12312273900283444 1107 86-87 -0.4785838595383147 1107 88-89 -0.11929563703988322 1107 90-91 0.3820820950050745 1107 92-93 0.3908324770145377 1107 94-95 0.6176145081071454 1107 96-97 0.40914842907746163 1107 98-99 0.8808224305876422 1107 100-101 1.4589646517365615 1108 1 -0.051961790398145524 1108 2 -0.1560792608230699 1108 3 -0.14817900526107763 1108 4 -0.4889604003444745 1108 5 -0.21487662621886017 1108 6 -0.061535918426280034 1108 7 -0.11975428633653706 1108 8 -0.30181788094130013 1108 9 0.05757807651578162 1108 10-11 0.1708731065771758 1108 12-13 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2316 96-97 -3.1355762804417786 2316 98-99 -3.212337946770848 2316 100-101 -4.134662093812953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 18.0 5 56.0 6 132.0 7 313.0 8 652.0 9 1065.0 10 1630.0 11 2369.0 12 3139.0 13 4233.0 14 4982.0 15 6258.0 16 7568.0 17 9296.0 18 8543.0 19 6435.0 20 6281.0 21 6551.0 22 7068.0 23 7747.0 24 8821.0 25 9621.0 26 10759.0 27 12470.0 28 14747.0 29 16946.0 30 19543.0 31 22625.0 32 26637.0 33 31191.0 34 37808.0 35 44867.0 36 48993.0 37 40895.0 38 14368.0 39 526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.98121181538018 24.381611909095824 16.373429812388487 19.263746463135508 2 19.127676589779764 25.237669491144676 35.22203452272911 20.41261939634645 3 21.48482779070708 27.236968613250184 31.799863418666714 19.478340177376023 4 15.92700087160456 21.09283037856392 34.571026786115425 28.40914196371609 5 12.597830872772692 39.12920414416519 34.35042996163143 13.92253502143069 6 29.085534059969987 38.356441337418794 16.82286659058847 15.735158012022751 7 24.59699521066772 35.91123111897851 21.872557036185068 17.619216634168698 8 25.03976134209131 34.78892792638985 20.902784641788497 19.268526089730344 9 24.972369236851797 17.157356072927243 21.824708641465016 36.04556604875594 10-11 24.157823324856903 27.635817556092697 27.65457502538436 20.55178409366604 12-13 25.453099587560317 25.905637574243634 28.195733630457635 20.44552920773841 14-15 22.39020385057323 27.45810724141843 27.64321151420462 22.508477393803723 16-17 20.01983596577341 30.003526893121663 28.81505376827189 21.16158337283304 18-19 19.794521471124728 27.685126113092938 32.52960759823523 19.990744817547107 20-21 20.99578574701902 26.49835113982514 32.77446558060545 19.73139753255039 22-23 20.830113488305223 26.812286030065863 32.66562733064364 19.691973150985273 24-25 20.852128242683463 27.129253565042365 32.730885352550565 19.287732839723603 26-27 20.242005049915086 26.963132025626972 33.03796871209194 19.756894212366003 28-29 19.953566839489977 27.425441867570022 33.09738608487811 19.52360520806189 30-31 20.490232637547287 26.942577433528918 32.615307892064806 19.95188203685899 32-33 20.45956922966331 27.178337481691813 32.56049564647 19.80159764217488 34-35 20.94213667350742 27.311337338936237 32.38000882895087 19.366517158605475 36-37 20.92657768830763 26.886165591414763 32.3345080034465 19.85274871683111 38-39 20.46015178134141 27.24403186599388 32.35979996135821 19.936016391306495 40-41 20.783506729540107 27.22897060838817 32.12184387826396 19.865678783807766 42-43 20.62584031513292 27.394526059456965 31.75939103057315 20.220242594836968 44-45 20.886602014157056 27.209316431127554 31.388478913801897 20.515602640913492 46-47 21.362197109503906 27.640326767359657 30.472303062910466 20.52517306022597 48-49 21.24650824262533 27.698972606201654 30.354561495926703 20.699957655246312 50-51 20.85372414807859 28.137695704085424 30.221012657374786 20.787567490461196 52-53 21.376775781973063 28.50955620052296 29.588166844881346 20.525501172622633 54-55 21.038467413670713 28.56335756453917 29.469781380010602 20.92839364177951 56-57 21.11754081715174 28.79114288024872 29.156071130120676 20.93524517247886 58-59 21.199541281827507 28.74064788887429 29.24216028852969 20.817650540768522 60-61 20.813766068931432 29.082056149297152 29.433621062444892 20.670556719326523 62-63 20.560882027873376 28.826860696025662 29.58760524400435 21.024652032096615 64-65 20.481134703339954 29.120577954694532 29.207401450278102 21.190885891687408 66-67 20.520003324714537 28.901990560506434 28.91603074013088 21.661975374648154 68-69 20.659344115357705 28.511786357320883 29.000156125569887 21.828713401751525 70-71 20.775019656295633 28.354374929798944 28.869369875322924 22.0012355385825 72-73 20.731608694480137 28.10812389364627 28.935698945987475 22.224568465886115 74-75 20.418572365642607 28.671634213623985 28.520563577712082 22.389229843021322 76-77 20.439374678971674 28.239927437764056 28.840341725870754 22.480356157393512 78-79 20.48128606611897 28.268421809552912 28.73705664515433 22.513235479173783 80-81 20.513370593679518 27.925154327921 28.66130075748726 22.90017432091222 82-83 20.533475905075974 27.809913827961434 28.612553801364015 23.04405646559858 84-85 20.276824304288834 27.666817924502872 28.695558410984013 23.36079936022428 86-87 20.304709515060058 27.459727023094416 28.530337139297384 23.705226322548146 88-89 20.1994094075831 27.491153502222044 28.31611335229896 23.993323737895896 90-91 20.176094313241187 27.132731567061512 28.459236676971294 24.231937442726007 92-93 20.222685979746426 27.130713727322558 28.282444805865808 24.364155487065208 94-95 20.037806971039366 26.852272012507978 28.483048639128754 24.626872377323906 96-97 19.861576615838043 26.56585556524005 28.601995704876494 24.970572114045414 98-99 19.82880139636599 26.70471329681426 28.229236749854824 25.237248556964925 100-101 20.12849456930732 25.90086599049769 28.655749121092654 25.314890319102336 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1035.0 1 722.5 2 419.5 3 451.5 4 429.0 5 434.5 6 644.5 7 1026.5 8 1370.5 9 1515.0 10 1308.5 11 1070.5 12 1078.0 13 1135.0 14 1213.0 15 1260.0 16 1296.5 17 1396.0 18 1468.5 19 1556.0 20 1774.0 21 2003.5 22 2214.5 23 2454.5 24 2822.0 25 3284.5 26 3947.0 27 4726.0 28 5307.5 29 5942.5 30 6715.0 31 7573.5 32 8629.5 33 9409.5 34 9933.0 35 10628.0 36 11450.5 37 12171.0 38 12548.5 39 12650.5 40 12691.5 41 13031.0 42 13261.5 43 13613.0 44 14458.5 45 14990.0 46 15088.0 47 15663.0 48 16747.0 49 16711.0 50 15646.5 51 14601.5 52 13465.5 53 12716.0 54 12257.0 55 11422.0 56 10294.5 57 9180.0 58 8225.5 59 7271.5 60 6129.5 61 4979.0 62 4090.0 63 3123.5 64 2223.0 65 1673.0 66 1241.5 67 963.5 68 765.5 69 557.0 70 404.0 71 294.0 72 232.5 73 195.0 74 148.5 75 102.5 76 67.5 77 45.5 78 27.5 79 20.5 80 16.0 81 8.0 82 7.5 83 5.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2830468420059485 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.369605261975577E-4 16-17 0.001123201753991859 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006626890348551968 36-37 0.014938583328091726 38-39 0.009434894733531617 40-41 0.006514570173152783 42-43 0.002358723683382904 44-45 0.0015724824555886027 46-47 0.0016848026309877885 48-49 1.1232017539918591E-4 50-51 1.1232017539918591E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.616008769959295E-4 60-61 7.862412277943013E-4 62-63 0.0 64-65 0.0 66-67 0.001123201753991859 68-69 3.369605261975577E-4 70-71 0.0013478421047902309 72-73 0.0 74-75 0.0 76-77 0.06660586401171724 78-79 0.03144964911177205 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.11299409645158101 88-89 0.2684452192040543 90-91 0.22890851746354088 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.1232017539918591E-4 100-101 2.2464035079837181E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 445156.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.15212400075507 #Duplication Level Percentage of deduplicated Percentage of total 1 87.34108895831864 60.3982181400663 2 7.487691743805576 10.355795758941461 3 2.0120088466178325 4.174040557557976 4 0.929253985165072 2.5703954724132347 5 0.5493339072201514 1.8993803234953595 6 0.3377171643097854 1.401231553412019 7 0.2552055884210336 1.2353605947323885 8 0.1909044058212381 1.056115611491258 9 0.12282076552193899 0.7643985126516708 >10 0.7211187486561028 8.603903667106334 >50 0.0333997317911007 1.6035335693836326 >100 0.01556492348124221 2.044836046344156 >500 0.0019456154351552763 1.002572865797206 >1k 0.0019456154351552763 2.890217326607065 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3410 0.7660235962224479 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3346 0.751646613771352 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2200 0.494208771756418 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 1490 0.334714122689574 RNA PCR Primer, Index 26 (100% over 22bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1357 0.30483695603339056 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1086 0.24395942096703177 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.19094429817861602 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 832 0.18690077186424536 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 828 0.18600221046105186 RNA PCR Primer, Index 26 (100% over 24bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.1617410525748277 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 639 0.14354518416015957 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 602 0.1352334911806198 RNA PCR Primer, Index 26 (100% over 29bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2464035079837181E-4 2 0.0 0.0 0.0 0.0 2.2464035079837181E-4 3 0.0 0.0 0.0 0.0 2.2464035079837181E-4 4 0.0 0.0 0.0 0.0 2.2464035079837181E-4 5 0.0 0.0 0.0 0.0 2.2464035079837181E-4 6 0.0 0.0 0.0 0.0 2.2464035079837181E-4 7 0.0 0.0 0.0 0.0 2.2464035079837181E-4 8 0.0 0.0 0.0 0.0 2.2464035079837181E-4 9 0.0 0.0 0.0 0.0 2.2464035079837181E-4 10-11 0.0 0.0 0.0 0.0 2.2464035079837181E-4 12-13 0.0 0.0 0.0 1.1232017539918591E-4 2.2464035079837181E-4 14-15 0.0 0.0 0.0 2.2464035079837181E-4 2.2464035079837181E-4 16-17 0.0 0.0 0.0 2.2464035079837181E-4 2.2464035079837181E-4 18-19 0.0 0.0 0.0 2.2464035079837181E-4 6.739210523951154E-4 20-21 0.0 0.0 0.0 4.4928070159674363E-4 6.739210523951154E-4 22-23 0.0 0.0 0.0 8.985614031934873E-4 6.739210523951154E-4 24-25 0.0 0.0 0.0 8.985614031934873E-4 6.739210523951154E-4 26-27 0.0 0.0 0.0 0.0020217631571853465 6.739210523951154E-4 28-29 0.0 0.0 0.0 0.00628992982235441 6.739210523951154E-4 30-31 0.0 0.0 0.0 0.021790114027442065 6.739210523951154E-4 32-33 0.0 0.0 0.0 0.05402600436700842 6.739210523951154E-4 34-35 0.0 0.0 0.0 0.10659184645382742 6.739210523951154E-4 36-37 0.0 0.0 0.0 0.1904950174770193 6.739210523951154E-4 38-39 0.0 0.0 0.0 0.304499995507193 6.739210523951154E-4 40-41 0.0 0.0 0.0 0.43636388142583726 6.739210523951154E-4 42-43 0.0 0.0 0.0 0.5753039383946301 6.739210523951154E-4 44-45 0.0 0.0 0.0 0.7701794427122177 6.739210523951154E-4 46-47 0.0 0.0 0.0 1.009533736487883 6.739210523951154E-4 48-49 0.0 0.0 0.0 1.2327139250060652 6.739210523951154E-4 50-51 0.0 0.0 0.0 1.4724051793079278 6.739210523951154E-4 52-53 0.0 0.0 0.0 1.7131073151883833 6.739210523951154E-4 54-55 0.0 0.0 0.0 1.9898642273719775 6.739210523951154E-4 56-57 0.0 0.0 0.0 2.336821249180063 6.739210523951154E-4 58-59 0.0 0.0 0.0 2.707253187646578 6.739210523951154E-4 60-61 0.0 0.0 0.0 3.103069485753309 6.739210523951154E-4 62-63 0.0 0.0 0.0 3.5088822794705674 6.739210523951154E-4 64-65 0.0 0.0 0.0 3.934014143356486 6.739210523951154E-4 66-67 0.0 0.0 0.0 4.432491081778073 6.739210523951154E-4 68-69 2.2464035079837181E-4 0.0 0.0 4.981062818427697 6.739210523951154E-4 70-71 2.2464035079837181E-4 0.0 0.0 5.568834296291637 6.739210523951154E-4 72-73 2.2464035079837181E-4 0.0 0.0 6.179182129410814 6.739210523951154E-4 74-75 2.2464035079837181E-4 0.0 0.0 6.797392374807933 6.739210523951154E-4 76-77 2.2464035079837181E-4 0.0 0.0 7.470414865799855 6.739210523951154E-4 78-79 2.2464035079837181E-4 0.0 0.0 8.246771918159027 6.739210523951154E-4 80-81 2.2464035079837181E-4 0.0 0.0 9.076256413482014 6.739210523951154E-4 82-83 2.2464035079837181E-4 0.0 0.0 9.905516268454205 6.739210523951154E-4 84-85 2.2464035079837181E-4 0.0 0.0 10.74095373307335 6.739210523951154E-4 86-87 2.2464035079837181E-4 0.0 0.0 11.600090754701723 6.739210523951154E-4 88-89 2.2464035079837181E-4 0.0 0.0 12.493934710528444 6.739210523951154E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 60 0.003963974 31.64117 5 GCGTGCC 110 2.7456772E-6 30.202932 9 ATAGCGT 90 8.981039E-4 26.367641 6 AGCGTGC 115 1.2870487E-4 24.762655 8 GTATCAA 4710 0.0 23.853601 1 GGTATCA 3530 0.0 23.735634 1 AGTACTT 3370 0.0 22.040844 12-13 GAGTACT 3275 0.0 21.810669 12-13 CTATAGC 135 3.81158E-4 21.094114 4 TAAGGTG 140 4.8717053E-4 20.340754 5 GTAAGGT 140 4.8717053E-4 20.340754 3 TATCAAC 5555 0.0 20.249208 2 GTACTTT 3505 0.0 20.244028 14-15 AGAGTAC 4440 0.0 20.096416 10-11 ATCAACG 5640 0.0 19.859882 3 ATATAAC 120 0.0046979305 19.835886 1 CAACGCA 5685 0.0 19.70268 5 TCAACGC 5690 0.0 19.685366 4 AACGCAG 5800 0.0 19.312025 6 GTATACA 125 0.006053563 18.984701 4 >>END_MODULE