FastQCFastQC Report
Thu 26 May 2016
SRR938826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424415
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35460.8355029864637207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.6566685908839226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.4274118492513224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13050.30748206354629315No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.1995688182557167No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7490.17647821118480733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.14608343248942662No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.13029699704298858No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.11545303535454685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.039.5294951
GTATCAA29850.035.3554461
ATCAACG35650.029.2950863
GCGGTAT650.005816729329.2546081
TAAACGT650.005857195229.2131444
ACGATCA650.00586396129.2062449
TATCAAC36200.028.8499952
TCAACGC36250.028.8102024
CAACGCA36300.028.770525
AACGCAG36900.028.16746
TACATGG15200.026.8587952
GTACATG16300.026.2483841
TTAGGAC1858.991265E-925.6601923
ACGCAGA40450.025.5780247
CGCAGAG40550.025.2808678
ACATGGG15600.024.9528923
GTATATA1951.5503247E-824.3788411
GCAGAGT42100.024.1246349
GAGTACT26200.024.09378612-13
GACTATT1601.8685314E-623.730079
Produced by FastQC (version 0.11.5)