##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938826_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 424415 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.227546151761835 33.0 31.0 34.0 31.0 34.0 2 32.356719248848414 34.0 31.0 34.0 31.0 34.0 3 32.369819634084564 34.0 31.0 34.0 31.0 34.0 4 35.86042199262514 37.0 37.0 37.0 35.0 37.0 5 35.86465841216734 37.0 37.0 37.0 35.0 37.0 6 35.93980655726117 37.0 37.0 37.0 35.0 37.0 7 35.89141288597246 37.0 37.0 37.0 35.0 37.0 8 35.88264788002309 37.0 37.0 37.0 35.0 37.0 9 37.61861150053603 39.0 38.0 39.0 35.0 39.0 10-11 37.53618745803047 39.0 38.0 39.0 35.0 39.0 12-13 37.465679818102565 39.0 38.0 39.0 35.0 39.0 14-15 38.80854470270843 40.0 39.0 41.0 36.0 41.0 16-17 38.70199451009036 40.0 39.0 41.0 36.0 41.0 18-19 38.65969982210808 40.0 38.5 41.0 36.0 41.0 20-21 38.567139474335264 40.0 38.5 41.0 35.0 41.0 22-23 38.558519373726185 40.0 39.0 41.0 35.0 41.0 24-25 38.49683917863412 40.0 38.5 41.0 35.0 41.0 26-27 38.35720815711038 40.0 38.0 41.0 35.0 41.0 28-29 38.177946114062884 40.0 38.0 41.0 35.0 41.0 30-31 37.902678981657104 40.0 38.0 41.0 34.0 41.0 32-33 37.65386355336169 40.0 37.5 41.0 34.0 41.0 34-35 37.34630491382255 40.0 37.0 41.0 33.0 41.0 36-37 37.11071121425963 40.0 37.0 41.0 33.0 41.0 38-39 36.90928336651626 40.0 36.5 41.0 33.0 41.0 40-41 36.74532356302204 40.0 36.5 41.0 32.0 41.0 42-43 36.64006573754462 40.0 36.5 41.0 31.5 41.0 44-45 36.375171706937785 40.0 36.0 41.0 31.0 41.0 46-47 35.929977734057466 40.0 36.0 41.0 29.5 41.0 48-49 35.5118033057267 40.0 35.5 41.0 26.5 41.0 50-51 34.36419070956493 38.5 34.0 40.0 23.0 40.5 52-53 34.40186963231743 38.5 35.0 39.5 23.0 40.5 54-55 34.823918805885754 39.0 35.0 40.5 23.0 41.0 56-57 34.66696629478223 39.0 35.0 41.0 21.5 41.0 58-59 34.43673173662571 39.0 35.0 41.0 20.0 41.0 60-61 34.12374916060931 38.0 35.0 40.0 17.5 41.0 62-63 33.739860749502256 38.0 34.5 40.0 10.5 41.0 64-65 33.33680242215756 37.0 34.0 40.0 6.0 41.0 66-67 32.93332233780616 36.5 34.0 39.5 2.0 41.0 68-69 32.49665539625131 36.0 33.5 39.0 2.0 41.0 70-71 32.06197118386485 35.5 33.0 39.0 2.0 40.5 72-73 31.634103412933094 35.0 33.0 38.0 2.0 40.0 74-75 31.179884075727767 35.0 32.0 37.0 2.0 39.5 76-77 30.730923742092056 35.0 32.0 37.0 2.0 39.0 78-79 30.282306233285816 35.0 31.0 36.0 2.0 39.0 80-81 29.814608343248942 35.0 31.0 36.0 2.0 37.5 82-83 29.415953724538483 34.5 31.0 35.0 2.0 37.0 84-85 29.070855177126163 34.0 30.0 35.0 2.0 37.0 86-87 28.675536915519007 34.0 30.0 35.0 2.0 36.0 88-89 28.23713582224945 34.0 29.0 35.0 2.0 36.0 90-91 27.803787566414947 34.0 29.0 35.0 2.0 35.5 92-93 27.376630185078284 34.0 27.5 35.0 2.0 35.0 94-95 26.915580269311874 34.0 26.5 35.0 2.0 35.0 96-97 26.38093611206013 34.0 25.0 35.0 2.0 35.0 98-99 25.70914317354476 33.0 22.0 35.0 2.0 35.0 100-101 23.352580610958615 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49705757498558967 1101 2 0.4410019563389156 1101 3 0.45441863556504103 1101 4 0.34934692959050295 1101 5 0.216837740442827 1101 6 0.20551414677532875 1101 7 0.3803929871525398 1101 8 0.4202104052726483 1101 9 0.560354119602998 1101 10-11 0.4823522694609679 1101 12-13 0.4792802716848499 1101 14-15 0.36996118005020406 1101 16-17 0.5539142145557889 1101 18-19 0.5903847386263905 1101 20-21 0.6254360387613715 1101 22-23 0.6026314982731336 1101 24-25 0.5325492935182297 1101 26-27 0.4579482883171906 1101 28-29 0.5814407875810232 1101 30-31 0.4739657625403382 1101 32-33 0.5593243092694422 1101 34-35 0.6533433868317218 1101 36-37 0.47902253107293546 1101 38-39 0.5998858052622538 1101 40-41 0.667707768224922 1101 42-43 0.8314214828293629 1101 44-45 0.7207054255218566 1101 46-47 0.4612324673308237 1101 48-49 0.40500834234122607 1101 50-51 0.47848214905441466 1101 52-53 0.5411434761734242 1101 54-55 0.8017933928554797 1101 56-57 0.8210604599081321 1101 58-59 0.8274174486667292 1101 60-61 0.649582305616633 1101 62-63 0.5956988949214761 1101 64-65 0.5879274914938719 1101 66-67 0.20871837746427246 1101 68-69 0.48719327412687363 1101 70-71 0.1429333391609191 1101 72-73 0.10376435444159426 1101 74-75 0.1895608373448745 1101 76-77 0.310475856462034 1101 78-79 0.1470850607557459 1101 80-81 0.15369085267617422 1101 82-83 0.29668493623614367 1101 84-85 0.18756750922560173 1101 86-87 0.037446843843714106 1101 88-89 -0.09732693936752312 1101 90-91 -0.004188517572842443 1101 92-93 0.10639352036292493 1101 94-95 -0.4010161386022304 1101 96-97 -0.2172818847812117 1101 98-99 -0.4141990391766299 1101 100-101 0.2887925192359404 1102 1 0.16480610822418384 1102 2 0.10461667100461369 1102 3 -0.008204154690474752 1102 4 0.102686152375 1102 5 0.10012098143341319 1102 6 0.18253568488231764 1102 7 0.11836362311724002 1102 8 -0.008011908705519488 1102 9 0.18030207477914928 1102 10-11 0.19016187374746352 1102 12-13 0.137954569031713 1102 14-15 0.22677789968992812 1102 16-17 0.21269413792890646 1102 18-19 0.38523979091127813 1102 20-21 0.37804548172812247 1102 22-23 0.30964410617971794 1102 24-25 0.2865486789668097 1102 26-27 0.4837378428634338 1102 28-29 0.5231904346940652 1102 30-31 0.5619402394514452 1102 32-33 0.23668481089703874 1102 34-35 0.2523869911758325 1102 36-37 0.4742597575255303 1102 38-39 0.6630045577228572 1102 40-41 0.6701539709692739 1102 42-43 0.7717931904181086 1102 44-45 0.6934082995100042 1102 46-47 0.8838710054666237 1102 48-49 0.7809265685921787 1102 50-51 0.8135783167501387 1102 52-53 0.7600390120334666 1102 54-55 0.39637429759040543 1102 56-57 0.32360508690422307 1102 58-59 0.2400575231810862 1102 60-61 0.1381386859087428 1102 62-63 0.17131752735250672 1102 64-65 0.13540398970303613 1102 66-67 0.10856329925299946 1102 68-69 0.3376148179952878 1102 70-71 0.21856637514098765 1102 72-73 0.21658015225417415 1102 74-75 -0.13774541091470027 1102 76-77 -0.2582626807135249 1102 78-79 -0.4533796937597643 1102 80-81 -0.7642209207848047 1102 82-83 -0.47253659995037367 1102 84-85 -0.6845769871783105 1102 86-87 -0.6637683355764956 1102 88-89 -0.6017152206400276 1102 90-91 -0.4878476911932239 1102 92-93 -0.7078919195694731 1102 94-95 -0.8041666748943719 1102 96-97 -0.8079666295023102 1102 98-99 -0.9787509447661655 1102 100-101 -1.2612454101098507 1103 1 0.1628840450853417 1103 2 0.14997642804090106 1103 3 0.25278122923230484 1103 4 0.3785535300184222 1103 5 0.3019516066171022 1103 6 0.12339958695040565 1103 7 0.3339379327840035 1103 8 0.28578383942956975 1103 9 0.4227572771789312 1103 10-11 0.3647687138663329 1103 12-13 0.2799143270010447 1103 14-15 0.291346625110144 1103 16-17 0.3273298089948611 1103 18-19 0.39654357485645164 1103 20-21 0.4006297236095051 1103 22-23 0.2992570343869332 1103 24-25 0.0871742825272861 1103 26-27 0.2690872431517448 1103 28-29 0.1800881688055611 1103 30-31 0.46614265322685355 1103 32-33 0.3125409920561566 1103 34-35 0.2818104188862023 1103 36-37 0.20395096781883382 1103 38-39 0.0824248597480235 1103 40-41 0.1466320235274381 1103 42-43 0.4179446321987257 1103 44-45 0.3497415442045053 1103 46-47 0.6644525695221759 1103 48-49 0.7545985022716692 1103 50-51 0.7101834494051502 1103 52-53 0.6027074141551978 1103 54-55 0.6145541224587276 1103 56-57 0.4858024902529081 1103 58-59 0.5359596099258539 1103 60-61 0.5519607457819404 1103 62-63 0.3601019157212235 1103 64-65 0.17950647485602644 1103 66-67 0.3698822451040016 1103 68-69 0.29281721777508096 1103 70-71 0.17952096873193568 1103 72-73 0.3464663679824298 1103 74-75 0.29309063989867923 1103 76-77 0.16452589700640274 1103 78-79 0.012380057678281986 1103 80-81 -0.018644698385305247 1103 82-83 0.15347050048675825 1103 84-85 0.45644506523056805 1103 86-87 0.4446906083151241 1103 88-89 0.3187804103984888 1103 90-91 0.08935906588505205 1103 92-93 0.24302315976228073 1103 94-95 0.11687711735486062 1103 96-97 0.10624264211973156 1103 98-99 0.14671032706114673 1103 100-101 0.2063897524491054 1104 1 0.032932014447474955 1104 2 0.048625274724571454 1104 3 0.09343208847983675 1104 4 0.09822585335732015 1104 5 -0.03428333579035581 1104 6 0.02614194946590942 1104 7 0.15841989298213122 1104 8 0.08164538285112144 1104 9 0.22403124964784382 1104 10-11 0.1785406102681364 1104 12-13 0.2124641281870936 1104 14-15 0.1861046778080535 1104 16-17 0.19741197240333008 1104 18-19 0.2047345144111432 1104 20-21 0.1523418683577873 1104 22-23 0.028640466882997373 1104 24-25 -0.14233859885845135 1104 26-27 -0.24048220495634354 1104 28-29 -0.11362647699296957 1104 30-31 -0.0406082284910525 1104 32-33 0.04475031823804443 1104 34-35 0.07486805050884726 1104 36-37 -0.10617926264904298 1104 38-39 0.0034732492084401656 1104 40-41 0.24730418078097216 1104 42-43 0.2720044424706174 1104 44-45 -0.0023887448817347945 1104 46-47 -0.09594242056154911 1104 48-49 -0.07817551416101054 1104 50-51 0.20157631945799892 1104 52-53 0.1229820411958471 1104 54-55 0.03049294442498507 1104 56-57 -0.1677287777600327 1104 58-59 -0.06832246164717048 1104 60-61 0.052048673329636586 1104 62-63 0.010497101199504755 1104 64-65 0.23366740180777867 1104 66-67 0.023740798988932 1104 68-69 0.2528883413914542 1104 70-71 0.28194679207572193 1104 72-73 0.3134056100469742 1104 74-75 0.09202720505787809 1104 76-77 0.11765074435441392 1104 78-79 0.11008954506067781 1104 80-81 0.1671437674743821 1104 82-83 0.13637103489085334 1104 84-85 -0.09382262530354524 1104 86-87 0.0060567093145671436 1104 88-89 0.24011700682081738 1104 90-91 -0.06921093909750553 1104 92-93 0.15572087462301454 1104 94-95 -0.05348250631523399 1104 96-97 -0.05472583096955219 1104 98-99 -0.12496137101519622 1104 100-101 -0.3860953731407406 1105 1 -0.1402830024810271 1105 2 0.05471753755077913 1105 3 0.02583293939917297 1105 4 -0.14641844837947104 1105 5 -0.3671605816880614 1105 6 -0.36328348023836554 1105 7 -0.3362368928997981 1105 8 -0.3233402853502625 1105 9 -0.18685692237369267 1105 10-11 -0.166853239542597 1105 12-13 -0.23513242419138436 1105 14-15 -0.17453980370043354 1105 16-17 0.05014400272482078 1105 18-19 -0.1004528975756287 1105 20-21 -0.11176771247455264 1105 22-23 -0.2412517699761949 1105 24-25 -0.201266260727877 1105 26-27 -0.10185326371173176 1105 28-29 -0.270994120086236 1105 30-31 -0.3424249212912045 1105 32-33 -0.603314139900121 1105 34-35 -0.37759731847254585 1105 36-37 -0.29976341078091195 1105 38-39 -0.12617959168362347 1105 40-41 -0.38506271326359354 1105 42-43 -0.6659843118114424 1105 44-45 -0.7707828843246389 1105 46-47 -0.47326571997130173 1105 48-49 -0.4040741229278808 1105 50-51 -0.43593404238245625 1105 52-53 -0.36603940157183956 1105 54-55 -0.1132020629156969 1105 56-57 -0.17887961619521775 1105 58-59 -0.1352924098129975 1105 60-61 -0.05924498372590392 1105 62-63 -0.13805423859895427 1105 64-65 -0.2277990358164388 1105 66-67 -0.14978382473893248 1105 68-69 -0.04855382705462574 1105 70-71 -0.16829140541346632 1105 72-73 -0.2343886911904569 1105 74-75 -0.34666315818081017 1105 76-77 -0.4250000890871206 1105 78-79 -0.0329712677071079 1105 80-81 -0.08578251614542154 1105 82-83 -0.22502249532577423 1105 84-85 -0.46572281206635324 1105 86-87 -0.3917505754338002 1105 88-89 -0.427123098245751 1105 90-91 -0.4408314810092797 1105 92-93 -0.3695120795252187 1105 94-95 -0.420351582588367 1105 96-97 -0.2667091122023848 1105 98-99 -0.463561349043097 1105 100-101 0.4118062917448526 1106 1 -0.06269329696259263 1106 2 -0.16401050006367512 1106 3 -0.16390722392673496 1106 4 -0.1390935288051125 1106 5 -0.09491113848093846 1106 6 -0.017864029607331133 1106 7 -0.037434616235600515 1106 8 -0.23565406906716646 1106 9 -0.09223182957487808 1106 10-11 -0.13429446696155622 1106 12-13 -0.11337543334753519 1106 14-15 0.035202834260466886 1106 16-17 0.1007502882976965 1106 18-19 0.042796298167992575 1106 20-21 -0.036058729549537816 1106 22-23 -0.1430087608200452 1106 24-25 -0.17740088087140293 1106 26-27 -0.35012844312276314 1106 28-29 -0.20187261550817226 1106 30-31 -0.3636176953570285 1106 32-33 -0.24778580532947103 1106 34-35 -0.0885599514841715 1106 36-37 -0.08816232194151752 1106 38-39 0.09209806236240325 1106 40-41 0.10528126100020785 1106 42-43 0.27635421825537065 1106 44-45 0.3001772740519968 1106 46-47 0.21578154555702866 1106 48-49 0.5289993737313594 1106 50-51 0.25419216400209876 1106 52-53 0.08610610696565146 1106 54-55 0.18302906799249996 1106 56-57 0.28780684755138 1106 58-59 0.297397518422585 1106 60-61 0.37176466735056835 1106 62-63 0.12620213358615473 1106 64-65 0.29641279293881695 1106 66-67 0.28288878105171733 1106 68-69 0.44815490840689165 1106 70-71 0.40629656786047263 1106 72-73 0.27019185817851366 1106 74-75 0.15359172922329734 1106 76-77 0.20646489482775365 1106 78-79 -0.11690098807335048 1106 80-81 0.2269544301550006 1106 82-83 0.20669490135821533 1106 84-85 0.39067164475126503 1106 86-87 0.24534420308636484 1106 88-89 0.34429239296929737 1106 90-91 0.7371368436628316 1106 92-93 0.6958554037709952 1106 94-95 0.7584457448058401 1106 96-97 0.535109744516248 1106 98-99 0.26013080636397845 1106 100-101 0.5920760269589067 1107 1 -0.33657805020218134 1107 2 -0.1570184956523164 1107 3 -0.3200197135876124 1107 4 -0.24226419326609516 1107 5 -0.16260344617324307 1107 6 -0.3190716392020363 1107 7 -0.3193811285046948 1107 8 -0.26404668004042264 1107 9 -0.35990759466999833 1107 10-11 -0.3342641388667076 1107 12-13 -0.2621044104636141 1107 14-15 -0.25561326720079336 1107 16-17 -0.22169250188703415 1107 18-19 -0.2772726293202865 1107 20-21 -0.2921050488216608 1107 22-23 -0.26514827005632213 1107 24-25 -0.2807038861098903 1107 26-27 -0.4063291599686423 1107 28-29 -0.449195471863284 1107 30-31 -0.19927250649570993 1107 32-33 -0.16519137467052758 1107 34-35 -0.6259186501331939 1107 36-37 -0.49242479781689497 1107 38-39 -0.32183080446969115 1107 40-41 -0.3990087858704854 1107 42-43 -0.3071941688414057 1107 44-45 -0.14392253295103075 1107 46-47 -0.3610533041810484 1107 48-49 -0.24184429872521207 1107 50-51 -0.09129997826423164 1107 52-53 -0.09534964158687131 1107 54-55 -0.12681102023469037 1107 56-57 -0.39081460268297974 1107 58-59 -0.45460137873759976 1107 60-61 -0.686787855395508 1107 62-63 -0.643282288801494 1107 64-65 -0.42180612076396073 1107 66-67 -0.7721732536877397 1107 68-69 -0.41671964356443425 1107 70-71 -0.680626509062602 1107 72-73 -0.6681786959984919 1107 74-75 -0.648837275463773 1107 76-77 -0.7186109254093545 1107 78-79 -0.5829831496393858 1107 80-81 -0.7226430333134353 1107 82-83 -0.4335835656944447 1107 84-85 -0.16017754190437472 1107 86-87 -0.3814823753531158 1107 88-89 -0.354668180507403 1107 90-91 -0.19751714034599033 1107 92-93 0.23940379086777241 1107 94-95 -0.022348299859171306 1107 96-97 -0.13432780717936765 1107 98-99 -6.025821113446739E-4 1107 100-101 0.9326908601543877 1108 1 -0.012355132711384442 1108 2 -0.04922789136801953 1108 3 -0.11811012247746078 1108 4 -0.01204038870849189 1108 5 -0.03123964089639486 1108 6 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-0.2983982769493636 2316 78-79 -0.026864720351621685 2316 80-81 0.09243085613587354 2316 82-83 0.30596624985804866 2316 84-85 0.24115961046178214 2316 86-87 -0.04183021168448775 2316 88-89 0.2543583342815303 2316 90-91 -0.02719220348520679 2316 92-93 0.6793865610410244 2316 94-95 0.43013713946459475 2316 96-97 -0.21825326240759324 2316 98-99 -0.7368095167484512 2316 100-101 -0.3733410518409599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4140.0 3 661.0 4 507.0 5 431.0 6 496.0 7 516.0 8 608.0 9 666.0 10 714.0 11 1015.0 12 1507.0 13 2318.0 14 2487.0 15 3083.0 16 3940.0 17 5839.0 18 5184.0 19 2931.0 20 2591.0 21 2514.0 22 2591.0 23 2727.0 24 2822.0 25 2945.0 26 3240.0 27 3721.0 28 4445.0 29 5373.0 30 6383.0 31 7962.0 32 10899.0 33 15344.0 34 22511.0 35 37178.0 36 60891.0 37 95952.0 38 84868.0 39 12413.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.95194280546918 19.945128760887478 15.037647242153579 23.065281191489763 2 15.57915199097873 21.459256830240133 39.35506396751964 23.60652721126149 3 18.280237192389645 25.375479894570674 32.13187632583042 24.212406587209262 4 12.592596087608873 17.77962838889885 37.6282190410584 31.99955648243387 5 12.739224850115658 36.86942359439173 35.25350516923949 15.137846386253129 6 29.27298898039123 36.2849524528658 18.54149460817858 15.900563958564383 7 25.06290688836329 34.3063246798004 22.83838712875494 17.792381303081374 8 24.410896815467055 33.37433016217747 21.94825429050306 20.26651873185241 9 24.96532626335057 16.860163414726195 22.78913650601955 35.385373815903684 10-11 23.33473744821589 26.663079684109547 28.712025477015583 21.29015739065898 12-13 24.5973946287818 25.132392504837874 28.97968093642328 21.290531929957048 14-15 21.961672029971595 26.385690828276726 28.374281615974784 23.27835552577689 16-17 20.051410846342495 29.249512636039782 29.152453114465292 21.546623403152427 18-19 19.55852640722068 27.40516248418588 32.39876602654884 20.6375450820446 20-21 20.58749615072862 26.67247154488811 32.207989258615584 20.53204304576769 22-23 20.225124902185033 26.627387245754857 32.32384749481566 20.823640357244443 24-25 20.239736212276423 26.803981363029123 32.31753659653413 20.63874582816033 26-27 19.73636649286401 26.925866512945422 32.564608991275215 20.77315800291535 28-29 19.684419124281373 27.547841408859224 32.28828611784269 20.47945334901672 30-31 19.903582649599798 27.179751175698957 31.998611476805404 20.918054697895844 32-33 19.74840081263022 27.29720227744102 31.886769747692945 21.06762716223582 34-35 20.400613893414587 27.454598943054183 31.483776602343333 20.6610105611879 36-37 20.38148279037003 27.199027972961954 31.639338255717337 20.780150980950683 38-39 19.960695475016337 27.548383208482967 31.583891998822995 20.9070293176777 40-41 20.149935203703727 27.574633874345984 31.573693530648 20.701737391302295 42-43 19.990002871515628 27.740022664884673 30.903298461553007 21.366676002046695 44-45 20.09057249522261 27.81298859689449 30.89320978097859 21.20322912690431 46-47 20.41047871850085 27.967910520083105 30.349802749919043 21.271808011496997 48-49 20.35581812636969 27.997716295860254 30.13732957916959 21.509135998600463 50-51 20.053377649232253 28.44676477784331 30.07551589923491 21.424341673689522 52-53 20.39974469292232 28.468748522528486 29.61443903361543 21.517067750933762 54-55 20.242741281619125 28.39014000940803 29.732793120321105 21.634325588651745 56-57 20.244961625042095 28.56703948573421 29.448570466348794 21.7394284228749 58-59 20.308130695048433 28.13804330747266 29.772971874597587 21.780854122881323 60-61 20.152330606310773 28.095891041925757 30.19908800051021 21.55269035125326 62-63 19.929260472959797 28.256340381681465 30.26803261156011 21.54636653379863 64-65 19.834899303470355 28.438435385408344 29.94427289965339 21.782392411467914 66-67 19.779498777029193 28.3160596341609 29.582180209979775 22.322261378830135 68-69 19.86075775774829 28.097897534355404 29.784220631212584 22.25712407668372 70-71 20.00815723363381 27.922565390867444 29.609930545293334 22.45934683020541 72-73 19.986807543815267 27.840456392785285 29.77936917362738 22.393366889772064 74-75 19.60381011823282 28.317836110066928 29.575936394005375 22.502417377694876 76-77 19.656605388130227 28.03309844672266 29.610486926799055 22.699809238348056 78-79 19.809447959181263 27.942472804444872 29.68598971023706 22.562089526136802 80-81 20.020420800854648 27.854421434277636 29.13343953410321 22.991718230764505 82-83 19.81595904107711 27.66050990644309 29.64558051141924 22.877950541060564 84-85 19.74627532460165 27.499958634415695 29.52025377195035 23.233512269032307 86-87 19.647979124096047 27.478097673797453 29.61603416916526 23.25788903294124 88-89 19.81507724419472 27.339398144153705 29.246066875235645 23.59945773641593 90-91 19.56722187415034 27.292329152530353 29.391202581773907 23.7492463915454 92-93 19.82715004444781 26.999087401410982 29.488046377030884 23.685716177110326 94-95 19.698019345487904 26.637521545558364 29.501041516429005 24.163417592524723 96-97 19.704383414923637 26.415515910404746 29.73118349483918 24.148917179832438 98-99 19.71547430603417 26.37773540339922 29.527402207592157 24.379388082974447 100-101 19.816852284559737 25.827657243352682 30.03866005273652 24.316830419351064 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1229.0 1 845.0 2 499.0 3 525.5 4 488.5 5 494.0 6 681.0 7 1058.0 8 1494.0 9 1623.5 10 1362.0 11 1175.5 12 1148.5 13 1160.0 14 1214.5 15 1280.0 16 1323.5 17 1362.5 18 1452.5 19 1554.5 20 1712.0 21 1947.5 22 2182.0 23 2471.0 24 2781.0 25 3200.5 26 3827.0 27 4537.0 28 5137.5 29 5721.0 30 6393.0 31 7229.0 32 8269.0 33 8892.5 34 9397.5 35 10076.5 36 10940.5 37 11670.5 38 11933.5 39 12004.0 40 11959.0 41 12145.0 42 12474.5 43 12931.5 44 13485.0 45 13962.5 46 14159.5 47 14508.0 48 15685.5 49 15875.0 50 14509.5 51 13186.5 52 12341.5 53 11981.5 54 11597.5 55 10829.5 56 9757.0 57 8561.5 58 7873.5 59 7301.0 60 6148.0 61 4961.0 62 3900.5 63 2924.5 64 2206.0 65 1680.5 66 1274.0 67 999.5 68 799.0 69 602.0 70 454.5 71 353.5 72 293.0 73 230.0 74 170.5 75 141.5 76 110.0 77 89.5 78 75.0 79 51.5 80 25.5 81 21.0 82 24.0 83 16.5 84 14.0 85 10.5 86 6.5 87 5.0 88 5.5 89 4.5 90 3.5 91 3.0 92 1.0 93 1.0 94 1.5 95 2.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.206872989880188 2 0.12440653605551169 3 0.14702590624742293 4 0.12511339137400893 5 0.17789192182180177 6 0.147261524686922 7 0.18213305373278513 8 0.18967284379675553 9 0.10909133748807183 10-11 0.15562597928913915 12-13 0.15904244666187575 14-15 0.12888328640599414 16-17 0.22701836645735896 18-19 0.17506450054781286 20-21 0.14926428142266412 22-23 0.18460704734752542 24-25 0.17459326366881472 26-27 0.2681337841499476 28-29 0.244336321760541 30-31 0.22230599766737744 32-33 0.14278477433643957 34-35 0.2861585947716268 36-37 0.3255068741679724 38-39 0.3083067280845399 40-41 0.2746132912361721 42-43 0.30477245149205373 44-45 0.3130190968745214 46-47 0.31761365644475337 48-49 0.33410694720968864 50-51 0.32786305856296316 52-53 0.32868772310120986 54-55 0.32326849899273113 56-57 0.3033587408550593 58-59 0.2793256600261536 60-61 0.2496377366492702 62-63 0.3094848202820353 64-65 0.34423854010814886 66-67 0.346241296843891 68-69 0.34942214577712855 70-71 0.3481262443598836 72-73 0.34070426351566274 74-75 0.338348079120672 76-77 0.32373973587172933 78-79 0.30064912880081995 80-81 0.31030948482028203 82-83 0.319734222400245 84-85 0.3199698408397441 86-87 0.3165533734670075 88-89 0.32362192665197975 90-91 0.3416467372736591 92-93 0.3409398819551618 94-95 0.34730157982163684 96-97 0.3460056784043919 98-99 0.3452988230858947 100-101 0.32244383445448443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 424415.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.62485443917086 #Duplication Level Percentage of deduplicated Percentage of total 1 86.87268951488998 60.48498362213499 2 7.8607313494427595 10.946045519807585 3 2.03749193420836 4.255802380207252 4 0.9564081512214286 2.6635911325291395 5 0.5544706072994408 1.9302467662011114 6 0.3564411483369029 1.4890297841452662 7 0.25018836028435654 1.219352971702121 8 0.19154095426155032 1.066880884768026 9 0.1416192941254282 0.8874200465334955 >10 0.7282162216976423 8.785346978379938 >50 0.032224554774557115 1.5270853846943684 >100 0.015264262747062083 1.857961753227307 >500 0.0013568233552944074 0.6539590815668505 >1k 0.0013568233552944074 2.232293694102517 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3546 0.8355029864637207 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2787 0.6566685908839226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1814 0.4274118492513224 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1305 0.30748206354629315 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 847 0.1995688182557167 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 749 0.17647821118480733 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.14608343248942662 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 553 0.13029699704298858 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 490 0.11545303535454685 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.356184394990752E-4 0.0 0.0 0.0 6 0.0 2.356184394990752E-4 0.0 0.0 0.0 7 0.0 2.356184394990752E-4 0.0 0.0 0.0 8 0.0 2.356184394990752E-4 0.0 0.0 0.0 9 0.0 2.356184394990752E-4 0.0 0.0 0.0 10-11 0.0 2.356184394990752E-4 0.0 0.0 0.0 12-13 0.0 2.356184394990752E-4 0.0 0.0 2.356184394990752E-4 14-15 0.0 2.356184394990752E-4 0.0 1.178092197495376E-4 2.356184394990752E-4 16-17 2.356184394990752E-4 2.356184394990752E-4 0.0 2.356184394990752E-4 2.356184394990752E-4 18-19 2.356184394990752E-4 2.356184394990752E-4 0.0 2.356184394990752E-4 2.356184394990752E-4 20-21 2.356184394990752E-4 2.356184394990752E-4 0.0 4.712368789981504E-4 2.356184394990752E-4 22-23 2.356184394990752E-4 2.356184394990752E-4 0.0 8.246645382467633E-4 2.356184394990752E-4 24-25 2.356184394990752E-4 2.356184394990752E-4 0.0 0.0018849475159926016 2.356184394990752E-4 26-27 2.356184394990752E-4 2.356184394990752E-4 0.0 0.002356184394990752 2.356184394990752E-4 28-29 2.356184394990752E-4 2.356184394990752E-4 0.0 0.006008270207226418 2.356184394990752E-4 30-31 2.356184394990752E-4 2.356184394990752E-4 0.0 0.020263185796920467 2.356184394990752E-4 32-33 2.356184394990752E-4 2.356184394990752E-4 0.0 0.04818397087756088 2.356184394990752E-4 34-35 3.534276592486128E-4 2.356184394990752E-4 0.0 0.09672136941437037 2.356184394990752E-4 36-37 4.712368789981504E-4 2.356184394990752E-4 0.0 0.1704699409775809 2.356184394990752E-4 38-39 4.712368789981504E-4 2.356184394990752E-4 0.0 0.2779119493891592 3.534276592486128E-4 40-41 4.712368789981504E-4 2.356184394990752E-4 0.0 0.3882991882944759 4.712368789981504E-4 42-43 4.712368789981504E-4 2.356184394990752E-4 0.0 0.5130591520092362 4.712368789981504E-4 44-45 4.712368789981504E-4 2.356184394990752E-4 0.0 0.6827044284485704 7.068553184972256E-4 46-47 4.712368789981504E-4 2.356184394990752E-4 0.0 0.8805061084080441 0.0010602829777458384 48-49 4.712368789981504E-4 2.356184394990752E-4 0.0 1.0780721699280185 0.001178092197495376 50-51 4.712368789981504E-4 2.356184394990752E-4 0.0 1.3040302534076318 0.001178092197495376 52-53 4.712368789981504E-4 3.534276592486128E-4 0.0 1.5377637453907143 0.001178092197495376 54-55 4.712368789981504E-4 4.712368789981504E-4 0.0 1.8163825500983708 0.001178092197495376 56-57 4.712368789981504E-4 4.712368789981504E-4 0.0 2.1350564895208697 0.001178092197495376 58-59 4.712368789981504E-4 4.712368789981504E-4 0.0 2.472815522542794 0.001178092197495376 60-61 4.712368789981504E-4 4.712368789981504E-4 0.0 2.8419118080180956 0.001178092197495376 62-63 4.712368789981504E-4 4.712368789981504E-4 0.0 3.228326048796579 0.001178092197495376 64-65 4.712368789981504E-4 4.712368789981504E-4 0.0 3.6615105498156284 0.001178092197495376 66-67 4.712368789981504E-4 4.712368789981504E-4 0.0 4.147355772062721 0.001178092197495376 68-69 4.712368789981504E-4 4.712368789981504E-4 0.0 4.712015362322255 0.001178092197495376 70-71 4.712368789981504E-4 4.712368789981504E-4 0.0 5.306009448299424 0.0014137106369944512 72-73 4.712368789981504E-4 4.712368789981504E-4 0.0 5.895055547047112 0.0014137106369944512 74-75 4.712368789981504E-4 4.712368789981504E-4 0.0 6.533934945748854 0.0014137106369944512 76-77 4.712368789981504E-4 4.712368789981504E-4 0.0 7.222765453624401 0.0014137106369944512 78-79 4.712368789981504E-4 4.712368789981504E-4 0.0 7.979454072075681 0.0014137106369944512 80-81 4.712368789981504E-4 4.712368789981504E-4 0.0 8.821436565625625 0.0014137106369944512 82-83 4.712368789981504E-4 4.712368789981504E-4 0.0 9.661180684000328 0.0014137106369944512 84-85 4.712368789981504E-4 4.712368789981504E-4 0.0 10.505637171165016 0.0014137106369944512 86-87 4.712368789981504E-4 4.712368789981504E-4 0.0 11.382373384541076 0.0014137106369944512 88-89 4.712368789981504E-4 4.712368789981504E-4 0.0 12.327910182250863 0.0014137106369944512 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1840 0.0 39.529495 1 GTATCAA 2985 0.0 35.355446 1 ATCAACG 3565 0.0 29.295086 3 GCGGTAT 65 0.0058167293 29.254608 1 TAAACGT 65 0.0058571952 29.213144 4 ACGATCA 65 0.005863961 29.206244 9 TATCAAC 3620 0.0 28.849995 2 TCAACGC 3625 0.0 28.810202 4 CAACGCA 3630 0.0 28.77052 5 AACGCAG 3690 0.0 28.1674 6 TACATGG 1520 0.0 26.858795 2 GTACATG 1630 0.0 26.248384 1 TTAGGAC 185 8.991265E-9 25.660192 3 ACGCAGA 4045 0.0 25.578024 7 CGCAGAG 4055 0.0 25.280867 8 ACATGGG 1560 0.0 24.952892 3 GTATATA 195 1.5503247E-8 24.378841 1 GCAGAGT 4210 0.0 24.124634 9 GAGTACT 2620 0.0 24.093786 12-13 GACTATT 160 1.8685314E-6 23.73007 9 >>END_MODULE