FastQCFastQC Report
Thu 26 May 2016
SRR938826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424415
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34430.8112342871953159No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32900.7751846659519575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.5207167512929562No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT14210.3348138025281859RNA PCR Primer, Index 26 (100% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12720.2997066550428236No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10230.2410376636075539No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.20734422675918618No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT7800.18378238280927867RNA PCR Primer, Index 26 (100% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.16964527643933414No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG7140.1682315658023397RNA PCR Primer, Index 26 (100% over 29bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7100.1672890920443434No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT4950.11663112755204222No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG651.3165311E-436.58661
GGTATCA32250.030.528541
GGTCGTG650.00586641129.2037627
GAATAGG1151.2684958E-424.815261
GTATCAA43600.024.544911
ACTAGCC1050.002205772822.5981483
GTAGGTC1100.002889866721.5709613
GAGTACT35200.021.30383512-13
AGTACTT35900.020.75623512-13
TATCAAC52600.020.480112
GTACTTT36900.020.3866714-15
ATCAACG53850.020.0047133
TCAACGC54450.019.7842754
TAAGGTA1200.00478415119.7733824
CAACGCA54800.019.7445145
CTAGACA1456.1756023E-419.6370124
GTAAGGT1707.995361E-519.5407523
AGAGTAC46050.019.27326810-11
AACGCAG56400.019.1002456
GACCGAG1250.00605726418.9824477
Produced by FastQC (version 0.11.5)