##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938825_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 436681 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.340949113884047 33.0 31.0 34.0 30.0 34.0 2 31.329631928112285 33.0 31.0 34.0 28.0 34.0 3 31.31018523819447 33.0 31.0 34.0 28.0 34.0 4 34.870170673787044 37.0 35.0 37.0 32.0 37.0 5 34.91426464627497 37.0 35.0 37.0 32.0 37.0 6 35.05524398817443 37.0 35.0 37.0 32.0 37.0 7 34.991314025570155 37.0 35.0 37.0 32.0 37.0 8 34.965338542322655 37.0 35.0 37.0 32.0 37.0 9 36.445833457375066 39.0 37.0 39.0 32.0 39.0 10-11 36.349324793155645 39.0 37.0 39.0 32.0 39.0 12-13 36.24843421170145 39.0 37.0 39.0 32.0 39.0 14-15 37.25365884936601 40.0 37.0 41.0 32.0 41.0 16-17 37.22673072563267 40.0 37.0 41.0 32.0 41.0 18-19 37.289777892786724 40.0 37.0 41.0 32.0 41.0 20-21 37.221770583102995 40.0 37.0 41.0 31.5 41.0 22-23 37.0920580469496 40.0 37.0 41.0 31.0 41.0 24-25 37.02271223158324 40.0 36.0 41.0 31.0 41.0 26-27 36.859277138231334 40.0 36.0 41.0 31.0 41.0 28-29 36.704116964099654 39.5 36.0 41.0 30.0 41.0 30-31 36.38251034508028 39.0 35.5 41.0 30.0 41.0 32-33 35.991003730411904 39.0 35.0 41.0 30.0 41.0 34-35 35.50822797419627 38.0 35.0 41.0 28.0 41.0 36-37 35.13963625621449 38.0 35.0 40.0 25.5 41.0 38-39 34.86574982653241 38.0 35.0 40.0 25.0 41.0 40-41 34.590061165931196 38.0 35.0 40.0 24.0 41.0 42-43 34.146607019769576 38.0 34.0 40.0 20.5 41.0 44-45 33.710984677602184 38.0 33.5 40.0 12.0 41.0 46-47 33.13928703103639 38.0 33.0 40.0 3.5 41.0 48-49 32.59746473970702 38.0 33.0 40.0 2.0 41.0 50-51 31.343875735376624 37.0 31.5 39.0 2.0 40.5 52-53 31.406951527545278 37.5 31.0 39.5 2.0 40.5 54-55 31.85227202465873 38.0 32.0 40.0 2.0 41.0 56-57 31.6947749043352 38.0 31.5 40.0 2.0 41.0 58-59 31.449442499215674 37.5 31.0 40.0 2.0 41.0 60-61 31.121443341936107 37.0 31.0 40.0 2.0 41.0 62-63 30.757139651141223 36.0 30.0 40.0 2.0 41.0 64-65 30.34365131526217 36.0 29.5 39.0 2.0 41.0 66-67 29.87176680460107 35.0 29.0 39.0 2.0 41.0 68-69 29.40487678648716 35.0 28.0 39.0 2.0 40.0 70-71 28.915831007073812 35.0 27.5 37.5 2.0 40.0 72-73 28.43642040757441 35.0 26.0 37.0 2.0 39.5 74-75 27.961170740197076 34.5 26.0 37.0 2.0 39.0 76-77 27.48244370604629 34.0 25.5 36.0 2.0 39.0 78-79 26.99683292838479 34.0 24.5 35.5 2.0 37.5 80-81 26.477729738642168 34.0 21.5 35.0 2.0 37.0 82-83 25.959839104517943 34.0 19.0 35.0 2.0 37.0 84-85 25.484071896876667 33.0 15.0 35.0 2.0 36.0 86-87 25.068874075125777 33.0 6.0 35.0 2.0 36.0 88-89 24.628335100450904 33.0 2.0 35.0 2.0 35.5 90-91 24.122202477323263 33.0 2.0 35.0 2.0 35.0 92-93 23.60623544418008 32.0 2.0 35.0 2.0 35.0 94-95 23.033232496948575 32.0 2.0 35.0 2.0 35.0 96-97 22.402527245288894 31.0 2.0 35.0 2.0 35.0 98-99 21.7180962762291 31.0 2.0 35.0 2.0 35.0 100-101 19.85703179208621 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.054409518762394526 1101 2 0.058003748202310135 1101 3 0.0974183562472426 1101 4 0.12600446475575922 1101 5 -0.054607179842221854 1101 6 -0.06658052879735266 1101 7 -0.14361233864410394 1101 8 0.04721449531361799 1101 9 0.2267091624482802 1101 10-11 0.08371337478210705 1101 12-13 0.06400195298525801 1101 14-15 0.3152150857542324 1101 16-17 0.006394609803678009 1101 18-19 -0.13434125339961156 1101 20-21 0.15372099211572987 1101 22-23 0.17766923911770505 1101 24-25 0.0010569492352487941 1101 26-27 0.09881095655224215 1101 28-29 -0.17265554210280953 1101 30-31 -0.26807542005978036 1101 32-33 -0.16136269846727913 1101 34-35 -0.1341635142988835 1101 36-37 0.15174380473192883 1101 38-39 -0.013856916048645473 1101 40-41 0.11310045209514641 1101 42-43 -0.2095440821658272 1101 44-45 0.12440231728908913 1101 46-47 0.20667164927415627 1101 48-49 0.11971242568602491 1101 50-51 0.1654672571821898 1101 52-53 0.11408334335355264 1101 54-55 -0.02284745602157301 1101 56-57 0.08880071053766159 1101 58-59 -0.23623794866888304 1101 60-61 -0.26421909071947525 1101 62-63 -0.3471786657635114 1101 64-65 -0.2809113778406882 1101 66-67 -0.4012419116519368 1101 68-69 -0.579050351262989 1101 70-71 -0.8580160900270037 1101 72-73 -0.9262656595926515 1101 74-75 -1.0572440905738993 1101 76-77 -0.7649118761503964 1101 78-79 -0.486972199460709 1101 80-81 -0.8654317306309096 1101 82-83 -1.03717068798424 1101 84-85 -0.8735675133774983 1101 86-87 -1.1621882204916183 1101 88-89 -0.8948382228899483 1101 90-91 -0.8231348138759671 1101 92-93 -0.8160481109729076 1101 94-95 -0.7143840687439642 1101 96-97 -0.8957679061900485 1101 98-99 -0.7813498250279771 1101 100-101 -0.1679046525999972 1102 1 0.17562500747677134 1102 2 0.1103880520497924 1102 3 0.1248853884001555 1102 4 0.05485812208770824 1102 5 0.07828287794411892 1102 6 -0.005849309357436994 1102 7 -0.255073295822946 1102 8 -0.2039399967098916 1102 9 -0.010307432602843392 1102 10-11 0.154652896686585 1102 12-13 0.0406034248082463 1102 14-15 0.022091413111375857 1102 16-17 0.0729631137795792 1102 18-19 0.28060293586299423 1102 20-21 0.04985683940174823 1102 22-23 0.013543072278181967 1102 24-25 0.044362747625143584 1102 26-27 0.14905605459145477 1102 28-29 0.08061654758949288 1102 30-31 -0.3013550417321298 1102 32-33 -0.19299392778520286 1102 34-35 0.0515615313868949 1102 36-37 -0.09687018746444664 1102 38-39 -0.15877962064685391 1102 40-41 0.03577418153654577 1102 42-43 -0.10643621433356287 1102 44-45 -0.47500747181546643 1102 46-47 -0.2526186159503254 1102 48-49 0.03204203007186379 1102 50-51 0.20955712234739465 1102 52-53 -0.049990346575821576 1102 54-55 -0.1613340219325714 1102 56-57 0.07452699097427029 1102 58-59 0.15321480976446722 1102 60-61 0.04095574999766782 1102 62-63 0.03357218621455971 1102 64-65 0.24125857326318112 1102 66-67 0.13494554821866345 1102 68-69 -0.2796294494009821 1102 70-71 0.09702354547455272 1102 72-73 -0.08642518939953803 1102 74-75 -0.3579676545586885 1102 76-77 -0.8424048380706814 1102 78-79 -0.8304798796108486 1102 80-81 -0.6115391786478988 1102 82-83 -0.6690822057399579 1102 84-85 -0.8203497527215973 1102 86-87 -1.14020039513478 1102 88-89 -1.1401893860252237 1102 90-91 -1.1834752346790474 1102 92-93 -1.4742166050707972 1102 94-95 -1.4024397066162422 1102 96-97 -1.639538588266408 1102 98-99 -1.4221484618630669 1102 100-101 -1.2019652541706023 1103 1 0.2176659617895318 1103 2 0.4427675555753403 1103 3 0.43504910906083083 1103 4 0.36240182922573894 1103 5 0.14735705934081267 1103 6 0.2408545445979442 1103 7 0.2584735022066127 1103 8 0.0043933366234441 1103 9 0.19640801219576787 1103 10-11 0.39495160237575533 1103 12-13 0.47557396474836366 1103 14-15 0.566704085766645 1103 16-17 0.8750555024591904 1103 18-19 0.8328945421606875 1103 20-21 0.7054699467136345 1103 22-23 0.8948274825940246 1103 24-25 0.9451946321030675 1103 26-27 0.8759045526113525 1103 28-29 1.128960022910654 1103 30-31 0.881677654104827 1103 32-33 0.6671975626040592 1103 34-35 0.8378432854678337 1103 36-37 0.8581005939515691 1103 38-39 0.8620597495839917 1103 40-41 1.0082097074710603 1103 42-43 0.8851966457070048 1103 44-45 1.0805078377566417 1103 46-47 0.81134835014646 1103 48-49 0.7473600538330913 1103 50-51 0.6708183509767842 1103 52-53 0.742726740258913 1103 54-55 0.9077341225666089 1103 56-57 1.0983365147837958 1103 58-59 0.847409729111515 1103 60-61 0.9373263672100727 1103 62-63 0.827164623189347 1103 64-65 0.9624625721040942 1103 66-67 0.5945888883133215 1103 68-69 0.7225559075003964 1103 70-71 0.6368127370132335 1103 72-73 0.5892726759535023 1103 74-75 0.6478036194681636 1103 76-77 0.2694467991359275 1103 78-79 0.6447205453867326 1103 80-81 0.6097795212458514 1103 82-83 0.7562957040446463 1103 84-85 0.6129787510937206 1103 86-87 0.09861323376506093 1103 88-89 0.03486856819094086 1103 90-91 -0.1319503143660974 1103 92-93 0.16328580968820816 1103 94-95 0.16973140320699542 1103 96-97 0.37894639193256907 1103 98-99 0.49562899394684123 1103 100-101 0.46396491099891435 1104 1 -0.02299131848623759 1104 2 -0.01986156552773366 1104 3 -0.08066669058747067 1104 4 -0.129469509674216 1104 5 -0.038338593339432236 1104 6 -0.02290187506456931 1104 7 -0.14928133364800544 1104 8 -0.023975457702796632 1104 9 -0.2347720411412908 1104 10-11 -0.1537621359931549 1104 12-13 -0.06810885442968839 1104 14-15 0.024229502389644608 1104 16-17 0.02125785720886597 1104 18-19 -0.18802103854946495 1104 20-21 -0.04846919313893494 1104 22-23 0.3947226717775507 1104 24-25 0.496412184421132 1104 26-27 0.38131686671772513 1104 28-29 0.4264655254785268 1104 30-31 0.44135285703021054 1104 32-33 0.5988900243772264 1104 34-35 0.5533867295512636 1104 36-37 0.7467798612075072 1104 38-39 0.44297952272680163 1104 40-41 0.24070047829638241 1104 42-43 0.025308327454787616 1104 44-45 0.3830442595153656 1104 46-47 0.4488484957170371 1104 48-49 0.2527432538237733 1104 50-51 0.5794378760673276 1104 52-53 0.6998358250275665 1104 54-55 0.41653240832747684 1104 56-57 0.3085695441657563 1104 58-59 0.5951213596184495 1104 60-61 0.5156637182913677 1104 62-63 0.4798157545638837 1104 64-65 0.5738444444911011 1104 66-67 0.4934231591211784 1104 68-69 0.7666958182947461 1104 70-71 0.4701722730506894 1104 72-73 0.3574282384964462 1104 74-75 0.4763738834749667 1104 76-77 0.5474574898392071 1104 78-79 0.47511044162402527 1104 80-81 0.7503628516677701 1104 82-83 0.8054432427373044 1104 84-85 0.4048623179222588 1104 86-87 0.34753601148461044 1104 88-89 0.0464501634115706 1104 90-91 0.0189044760704995 1104 92-93 0.18076010192405434 1104 94-95 0.31089119311953795 1104 96-97 0.17656835096318702 1104 98-99 0.038531554824459135 1104 100-101 -0.0642757812183028 1105 1 0.6249178393636221 1105 2 0.5881685084946646 1105 3 0.47898030505534095 1105 4 0.6644691796254776 1105 5 0.5573950146020579 1105 6 0.5315353166241081 1105 7 0.5312264699850147 1105 8 0.6624729619161869 1105 9 0.5662699196495353 1105 10-11 0.6931022928384891 1105 12-13 0.618632997292984 1105 14-15 1.0061169905554692 1105 16-17 0.6252573921466578 1105 18-19 0.6853131806166317 1105 20-21 0.6869768513409511 1105 22-23 0.6309576610645138 1105 24-25 0.8915323531722805 1105 26-27 0.7147360151002573 1105 28-29 0.6193500737868547 1105 30-31 0.6671294832253949 1105 32-33 0.4826300103146295 1105 34-35 0.4597846567025954 1105 36-37 0.506004978995577 1105 38-39 0.6049734215536304 1105 40-41 0.29119848240443247 1105 42-43 0.10907212014777912 1105 44-45 0.5293893688880331 1105 46-47 0.6250849244822376 1105 48-49 0.35527009178834845 1105 50-51 0.7117010126904226 1105 52-53 0.5577150306570395 1105 54-55 0.6041324105420642 1105 56-57 0.3910765616221994 1105 58-59 0.35635344226664856 1105 60-61 0.4342778329292045 1105 62-63 0.29320677775609383 1105 64-65 0.34211612940038805 1105 66-67 0.4145840429608221 1105 68-69 0.3534409918902881 1105 70-71 0.5303563048077926 1105 72-73 0.34351235939046987 1105 74-75 0.4728056383650383 1105 76-77 0.7233755724541879 1105 78-79 0.27818981777630114 1105 80-81 0.4186101761759957 1105 82-83 0.4569538644258131 1105 84-85 0.31056818822527177 1105 86-87 0.7459725166528877 1105 88-89 0.7668398190994488 1105 90-91 0.8815561057259664 1105 92-93 0.8171257515439798 1105 94-95 0.6283238283059198 1105 96-97 0.7356450917629829 1105 98-99 1.1776931141115874 1105 100-101 1.4584735114996015 1106 1 -0.24385618212825833 1106 2 -0.030115278469018847 1106 3 -0.16370875739213275 1106 4 0.11152385058006331 1106 5 -0.21171852931735202 1106 6 -0.21113357617421258 1106 7 -0.028996740799023257 1106 8 -0.033884175338279476 1106 9 -0.34914081284488674 1106 10-11 -0.01832885582302879 1106 12-13 -0.35410996293919794 1106 14-15 -0.2890658249034459 1106 16-17 -0.14413628578886062 1106 18-19 -0.21497591768799396 1106 20-21 -0.2381685382134151 1106 22-23 -0.46972810700692946 1106 24-25 -0.46087343775811007 1106 26-27 -0.3150646415338798 1106 28-29 -0.3599032009797938 1106 30-31 -0.36010234448098544 1106 32-33 -0.29631623757035896 1106 34-35 -0.06363454704447946 1106 36-37 0.04645352958218041 1106 38-39 -0.342521059806252 1106 40-41 -0.02892466450905573 1106 42-43 -0.057428333669328424 1106 44-45 -0.05681627189051852 1106 46-47 0.14127386927343366 1106 48-49 0.1621961252027404 1106 50-51 0.3821401507740809 1106 52-53 0.21265155228898536 1106 54-55 0.27716278827476515 1106 56-57 0.22923193515289597 1106 58-59 0.011550488533583092 1106 60-61 0.13992141281782366 1106 62-63 0.11851800664799228 1106 64-65 -0.12367950723217547 1106 66-67 -0.312604644107072 1106 68-69 -0.17780235492682905 1106 70-71 -0.340230183768341 1106 72-73 -0.23395310008534054 1106 74-75 -0.07857934421062751 1106 76-77 -0.22771302833242757 1106 78-79 -0.029419742637401214 1106 80-81 -0.27607736234243063 1106 82-83 0.06652268214821433 1106 84-85 0.04530368568725862 1106 86-87 0.26101571331817297 1106 88-89 0.4043856210110839 1106 90-91 0.4837602001647454 1106 92-93 0.6498745385636902 1106 94-95 0.760222128452714 1106 96-97 0.5278668906715787 1106 98-99 0.5931906881510081 1106 100-101 0.13454614464729886 1107 1 -0.16005858102712267 1107 2 0.02319358522196424 1107 3 -0.11688871850288152 1107 4 -0.20071534017390746 1107 5 -0.14012297754876357 1107 6 -0.020521616636507645 1107 7 -0.48909156066312676 1107 8 -0.2764285811847742 1107 9 -0.3091591650766503 1107 10-11 -0.22301632168569085 1107 12-13 -0.052936730980093216 1107 14-15 -0.22606195782604033 1107 16-17 -0.1170885918341753 1107 18-19 -0.09486711517111956 1107 20-21 -0.27248065724074877 1107 22-23 -0.07440683371162038 1107 24-25 -0.3678887968282538 1107 26-27 -0.21283287906419446 1107 28-29 -0.5493189882686877 1107 30-31 -0.1830196938943658 1107 32-33 -0.16232309780937726 1107 34-35 -0.3318993872271321 1107 36-37 -0.23676073272364562 1107 38-39 0.11996064258172368 1107 40-41 0.16789209719595988 1107 42-43 0.05898817460009553 1107 44-45 -0.1875376893819407 1107 46-47 0.029210554073848982 1107 48-49 0.33389873165661044 1107 50-51 0.5084592174320086 1107 52-53 0.4120026698088104 1107 54-55 0.3543623250993946 1107 56-57 0.45009776722997685 1107 58-59 0.11634937866051942 1107 60-61 -0.12090968110627998 1107 62-63 -0.21295279364040098 1107 64-65 -0.25676049737877804 1107 66-67 0.02483609347786242 1107 68-69 0.054140359892596024 1107 70-71 0.008402138007458149 1107 72-73 0.12133736767809111 1107 74-75 0.0355322111410139 1107 76-77 0.10582239802923965 1107 78-79 0.04858270432683298 1107 80-81 -0.07918959497881772 1107 82-83 0.08422493903576012 1107 84-85 0.24250278555398452 1107 86-87 0.19570143134923867 1107 88-89 0.3983883738458438 1107 90-91 0.09440886416596683 1107 92-93 0.3353959385767986 1107 94-95 0.551847830316337 1107 96-97 0.5178277829396087 1107 98-99 0.4996216160311029 1107 100-101 0.3893253716687717 1108 1 -0.08884744817215662 1108 2 -0.23566703665235522 1108 3 -0.3270120372367664 1108 4 -0.18943959699992519 1108 5 -0.009535582773501972 1108 6 -0.11394254665235337 1108 7 0.13778384703419988 1108 8 0.027069073584897296 1108 9 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2316 86-87 -1.835208645959863 2316 88-89 -2.3026966661650015 2316 90-91 -2.428182573059946 2316 92-93 -2.960969177386854 2316 94-95 -2.8610957928130993 2316 96-97 -2.836544024104107 2316 98-99 -3.0161205061263168 2316 100-101 -3.1630165063546443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4358.0 3 829.0 4 688.0 5 686.0 6 744.0 7 936.0 8 1117.0 9 1373.0 10 1674.0 11 2617.0 12 3786.0 13 5244.0 14 5516.0 15 6337.0 16 7802.0 17 10070.0 18 7998.0 19 4687.0 20 4475.0 21 4343.0 22 4627.0 23 5102.0 24 5464.0 25 5865.0 26 6787.0 27 7672.0 28 8716.0 29 9808.0 30 11513.0 31 13491.0 32 16086.0 33 19622.0 34 24995.0 35 34745.0 36 46827.0 37 67712.0 38 61731.0 39 10634.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.463133508423844 19.758237473191656 14.920922551122223 18.857706467262283 2 15.404575253289323 23.542612203731718 38.397056800990235 22.655755741988724 3 21.468878640910216 27.53064461415749 31.58435176441466 19.416124980517637 4 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19.911087176960578 24-25 20.664107265642905 27.04194361677745 33.392848957139584 18.901100160440066 26-27 19.76880374791426 26.26164347140473 35.31237966884867 18.657173111832336 28-29 18.33279666678508 28.9900775893779 33.769656605983236 18.907469137853784 30-31 19.182695117046737 27.359525007132458 33.454938351526344 20.00284152429446 32-33 19.1537258630862 27.777084037416156 33.752785303906016 19.31640479559163 34-35 20.3527474193415 27.137226257252717 33.321044483339996 19.188981840065786 36-37 19.672027417716365 26.74182225653144 34.30746451699744 19.278685808754755 38-39 19.212012467938763 27.91794624064042 33.68160748996469 19.188433801456124 40-41 19.254075608897924 28.019883038245986 33.274423182619934 19.451618170236156 42-43 18.777153439579465 28.21967758076297 31.93353769705475 21.06963128260282 44-45 19.530991165102154 27.162479293208175 32.16178906681392 21.14474047487576 46-47 20.490382281448692 28.23471351611274 31.756014453324067 19.5188897491145 48-49 19.972754175468634 28.03558525595887 31.367100666878372 20.62455990169412 50-51 18.44554196880224 28.7270673205795 32.13008309662931 20.69730761398895 52-53 19.358320025403955 29.426708208883102 30.105297553028453 21.10967421268449 54-55 18.677529833635706 28.04390517200717 30.873973381252757 22.404591613104365 56-57 19.82276355439008 27.83670324109217 30.348607181468957 21.991926023048787 58-59 20.822802712703208 27.41058267006713 30.32519783002141 21.441416787208254 60-61 19.5987235666564 27.77817372552085 31.82714811868623 20.79595458913652 62-63 18.866472665370768 27.937451835152576 31.59176913078985 21.60430636868681 64-65 18.69518288290569 28.845857316121727 30.76582288677209 21.693136914200494 66-67 19.19732295225525 27.765394693084254 29.79481414057493 23.24246821408557 68-69 18.84133527598819 27.551633418266942 30.379991575691033 23.227039730053836 70-71 19.788046455319208 27.57099258427277 29.966488830377326 22.674472130030704 72-73 19.31748618034364 26.648002651164717 31.285814887946866 22.748696280544777 74-75 18.329210341613837 28.51181093302344 30.089631922311327 23.069346803051396 76-77 18.7723530379425 27.0670628525151 30.109549298334613 24.05103481120778 78-79 18.853766440767238 27.05549121913241 30.53540809672927 23.55533424337108 80-81 19.50433525512968 26.75264721408535 30.032927007391613 23.710090523393358 82-83 19.032074021928675 26.278405823107466 30.780662645336132 23.90885750962773 84-85 18.887029336830057 26.579593620777906 30.493624803740577 24.03975223865146 86-87 18.89129066807169 26.52407844346303 30.231126828129117 24.35350406033617 88-89 18.946491584335554 26.233240867833395 29.72572147826677 25.094546069564277 90-91 19.269645016504843 25.27685445020877 30.21012749399118 25.243373039295207 92-93 20.008192345219904 25.612239731953828 30.16566486864633 24.213903054179937 94-95 19.567441180768668 24.89986318566821 30.45574476360117 25.076950869961944 96-97 18.894704925936622 24.686411530712167 31.140448281059957 25.278435262291254 98-99 19.63422562031492 24.881747524780955 30.000172589358144 25.483854265545986 100-101 19.598179567532245 23.777558430084074 30.91525509523218 25.709006907151498 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1249.0 1 827.5 2 434.5 3 458.0 4 406.0 5 407.0 6 592.5 7 1007.0 8 1506.0 9 1667.5 10 1481.0 11 1355.0 12 1396.5 13 1465.0 14 1551.0 15 1636.0 16 1760.0 17 1931.0 18 2055.0 19 2146.5 20 2350.0 21 2605.5 22 2872.5 23 3221.5 24 3562.0 25 4071.5 26 4667.5 27 5281.5 28 5790.0 29 6315.0 30 6981.5 31 7571.5 32 8161.0 33 8844.5 34 9709.5 35 10201.5 36 10724.0 37 11506.5 38 11519.0 39 11073.5 40 10767.0 41 10914.0 42 11332.0 43 11785.0 44 12225.0 45 12623.0 46 14092.0 47 18339.5 48 25016.0 49 25911.0 50 19498.0 51 14041.0 52 11464.5 53 10338.0 54 9471.5 55 8424.0 56 7473.0 57 6562.0 58 5892.0 59 5363.5 60 4679.5 61 3927.5 62 3262.5 63 2571.0 64 1911.0 65 1490.0 66 1127.5 67 901.0 68 721.5 69 518.0 70 425.5 71 368.5 72 335.0 73 280.5 74 216.5 75 205.0 76 157.0 77 98.0 78 64.5 79 41.0 80 27.5 81 18.5 82 17.0 83 10.5 84 8.5 85 7.5 86 6.5 87 5.0 88 2.5 89 2.0 90 0.5 91 0.0 92 0.0 93 1.0 94 1.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16396408362168263 2 0.09640904916861508 3 0.08908104543133316 4 0.10671405442416776 5 0.12915606586959358 6 0.040075020438260424 7 0.04396802242369144 8 0.04854802475949263 9 0.04854802475949263 10-11 0.047288524117147304 12-13 0.05278452692010873 14-15 0.04866252481788766 16-17 0.04957852528504789 18-19 0.05553252832158945 20-21 0.05473102791282424 22-23 0.06835653486183278 24-25 0.08724904449701269 26-27 0.08816504496417293 28-29 0.07911954035096558 30-31 0.06766953451146261 32-33 0.054616527854429206 34-35 0.303883154980409 36-37 0.47437374193060844 38-39 0.4502142296092571 40-41 0.4209022146601295 42-43 0.45765673340493407 44-45 0.46738923836851154 46-47 0.4672747383101165 48-49 0.48525124747813625 50-51 0.4732287413466581 52-53 0.48158724560949523 54-55 0.47231274087949787 56-57 0.4467792278574062 58-59 0.4209022146601295 60-61 0.3936512007621124 62-63 0.45250423077715773 64-65 0.49647225320084915 66-67 0.4975027537264044 68-69 0.5092962597410925 70-71 0.503571256821341 72-73 0.4934952516825784 74-75 0.4868542482956666 76-77 0.46635873784295634 78-79 0.4352147219595082 80-81 0.4423137255800001 82-83 0.4577712334633291 84-85 0.45536673223703344 86-87 0.4693357393612271 88-89 0.4819307457846803 90-91 0.48353374660221077 92-93 0.4875412486460369 94-95 0.49246475115702304 96-97 0.4933807516241833 98-99 0.4862817480036915 100-101 0.4715112404707326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 436681.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.74820044116868 #Duplication Level Percentage of deduplicated Percentage of total 1 88.05256795675544 57.892978873803834 2 7.453033386141351 9.800470659334874 3 1.7562203895370114 3.4640499057044027 4 0.8020984258750296 2.109461123119093 5 0.4324316393629728 1.4215801050969952 6 0.2735333231769054 1.0790594255744488 7 0.1997112938947994 0.9191460726952292 8 0.1607263932899331 0.8453976897770104 9 0.1324496220905095 0.7837491871407476 >10 0.6679533548941711 7.9988564749299425 >50 0.04246961925121512 1.874961484279706 >100 0.01775369327617243 2.4450944239732406 >500 0.0034811163286612608 1.6234255124255543 >1k 0.005569786125858017 7.741769062144985 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4949 1.1333215779940047 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4894 1.1207265715705514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3762 0.861498439364204 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3214 0.7360063753632514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2478 0.5674622894057676 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1944 0.4451762270398758 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1575 0.3606751839443438 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1540 0.35266017985669174 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1443 0.33044716852805595 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1277 0.29243314914090607 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1245 0.28510514540362414 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1205 0.2759451407320218 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1161 0.26586913559325914 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1060 0.24274012379746313 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.2379311213448719 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1039 0.2379311213448719 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.20610010511105362 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 829 0.18984109681895936 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 819 0.1875510956510588 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 799 0.18297109331525757 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 710 0.1625900829209423 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 686 0.15709408011798084 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 671 0.15365907836612996 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 584 0.1337360682053948 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 550 0.12595006423453275 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.12480506365058247 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 484 0.11083605652638882 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 484 0.11083605652638882 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 455 0.1041950531394771 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.2900011679005957E-4 0.0 20-21 0.0 0.0 0.0 0.001030500525555268 0.0 22-23 0.0 0.0 0.0 0.0037785019270359828 0.0 24-25 0.0 0.0 0.0 0.009847005021972562 0.0 26-27 0.0 0.0 0.0 0.01957950998555009 0.0 28-29 0.0 0.0 0.0 0.05324252715368885 0.0 30-31 0.0 0.0 0.0 0.13969007124193633 0.0 32-33 0.0 0.0 0.0 0.30731815673225993 0.0 34-35 0.0 0.0 0.0 0.5637982875371266 0.0 36-37 0.0 0.0 0.0 0.9006574593353043 0.0 38-39 0.0 0.0 0.0 1.368962698170976 0.0 40-41 0.0 0.0 0.0 1.8918844648610769 0.0 42-43 0.0 0.0 0.0 2.3901887189962467 0.0 44-45 0.0 0.0 0.0 2.9705895150006527 0.0 46-47 0.0 0.0 0.0 3.604919838509118 0.0 48-49 0.0 0.0 0.0 4.211083147652405 0.0 50-51 0.0 0.0 0.0 4.847474472211982 0.0 52-53 0.0 0.0 0.0 5.4599352845669955 0.0 54-55 0.0 0.0 0.0 6.103769112922247 0.0 56-57 0.0 0.0 0.0 6.892789015322398 0.0 58-59 0.0 0.0 0.0 7.643909398393793 0.0 60-61 0.0 0.0 0.0 8.469340319363564 0.0 62-63 0.0 0.0 0.0 9.276222230873337 0.0 64-65 0.0 0.0 0.0 10.113904658091375 0.0 66-67 0.0 0.0 0.0 11.020859620638408 0.0 68-69 0.0 0.0 0.0 12.03372713720084 0.0 70-71 0.0 0.0 0.0 13.098692180333012 0.0 72-73 0.0 0.0 0.0 14.065072673187064 0.0 74-75 0.0 0.0 0.0 15.103473702771588 0.0 76-77 0.0 0.0 0.0 16.137180229961917 0.0 78-79 0.0 0.0 0.0 17.368971858175648 0.0 80-81 0.0 0.0 0.0 18.604312988199624 0.0 82-83 0.0 0.0 0.0 19.777251586398307 0.0 84-85 0.0 0.0 0.0 20.956258687691932 0.0 86-87 0.0 0.0 0.0 22.12770878513148 0.0 88-89 0.0 0.0 0.0 23.346332906629783 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCGTA 25 0.0050873477 56.8061 9 GACGTGT 45 9.781163E-4 42.078587 6 TAGCTAA 35 0.008314029 27.193827 32-33 TGTAGGG 90 9.075611E-4 26.320223 2 GTACATG 3150 0.0 24.989534 1 TACATGG 3115 0.0 24.33459 2 CATGGGG 1055 0.0 23.800468 4 CCTTTAG 160 1.8825776E-6 23.70995 1 CATTCCG 100 0.0016850987 23.669209 9 GGACACG 100 0.0016850987 23.669209 6 ACATGGG 3060 0.0 22.759253 3 TAGGCAT 130 2.989662E-4 21.866034 5 GAGTACT 3925 0.0 21.829943 12-13 CATGGGA 1600 0.0 21.615486 4 ACTGATC 115 0.0037937923 20.581919 8 AGTACTT 4035 0.0 20.35493 12-13 ATGGGGT 235 1.15926014E-7 20.160172 5 GTACTTT 4245 0.0 19.961308 14-15 ATGGGAG 600 0.0 19.74017 5 CACTGAT 120 0.004853583 19.724339 7 >>END_MODULE