##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938825_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 436681 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874278935882256 31.0 31.0 34.0 30.0 34.0 2 32.08012943086601 33.0 31.0 34.0 30.0 34.0 3 32.141460242144724 34.0 31.0 34.0 30.0 34.0 4 35.808395602281756 37.0 35.0 37.0 35.0 37.0 5 35.734078194379876 37.0 35.0 37.0 33.0 37.0 6 35.79930429764519 37.0 35.0 37.0 33.0 37.0 7 35.72970429214919 37.0 35.0 37.0 33.0 37.0 8 35.7211213677719 37.0 35.0 37.0 33.0 37.0 9 37.29203468893769 39.0 37.0 39.0 34.0 39.0 10-11 37.24117605299979 39.0 37.0 39.0 33.0 39.0 12-13 37.18807321591734 39.0 37.0 39.0 33.0 39.0 14-15 38.26602371067209 40.0 38.0 41.0 33.0 41.0 16-17 38.237553271152166 40.0 37.5 41.0 33.0 41.0 18-19 38.35417616062984 40.0 38.0 41.0 33.5 41.0 20-21 38.41286545556138 40.0 38.0 41.0 34.0 41.0 22-23 38.34593101142482 40.0 38.0 41.0 33.5 41.0 24-25 38.21802299619173 40.0 37.5 41.0 33.0 41.0 26-27 37.98017087988715 40.0 37.0 41.0 33.0 41.0 28-29 37.770113652757956 40.0 37.0 41.0 33.0 41.0 30-31 37.41541422686126 39.5 36.0 41.0 32.0 41.0 32-33 37.01161374092301 39.0 35.0 41.0 31.5 41.0 34-35 36.73137026799884 39.0 35.0 41.0 31.0 41.0 36-37 36.49141936562388 39.0 35.0 41.0 30.5 41.0 38-39 36.23700023587012 39.0 35.0 41.0 30.0 41.0 40-41 35.98007240983693 39.0 35.0 41.0 30.0 41.0 42-43 35.61215280719793 39.0 35.0 41.0 28.0 41.0 44-45 35.10223710214092 39.0 35.0 41.0 25.0 41.0 46-47 34.440603094707576 39.0 34.5 41.0 21.5 41.0 48-49 33.84426618057575 38.0 34.0 40.5 12.0 41.0 50-51 33.425360846934026 38.0 33.0 40.0 4.5 41.0 52-53 33.18887128132435 38.0 33.0 40.0 2.0 41.0 54-55 32.91720615277514 38.0 33.0 40.0 2.0 41.0 56-57 32.55204714654404 38.0 33.0 40.0 2.0 41.0 58-59 32.171138657280714 37.0 32.0 40.0 2.0 41.0 60-61 31.687094240418062 37.0 31.0 40.0 2.0 41.0 62-63 31.274606635049384 36.0 31.0 39.5 2.0 41.0 64-65 30.850740013877406 36.0 31.0 39.0 2.0 40.5 66-67 30.32530955090787 35.0 30.0 39.0 2.0 40.0 68-69 29.80053288327177 35.0 29.0 38.0 2.0 40.0 70-71 29.282476224062876 35.0 29.0 37.5 2.0 39.5 72-73 28.93348806107891 35.0 28.5 37.0 2.0 39.0 74-75 28.542534481692584 34.0 28.0 36.5 2.0 39.0 76-77 26.632545267598086 32.0 25.0 34.5 2.0 36.5 78-79 27.536792303764074 34.0 26.0 35.0 2.0 37.0 80-81 27.51603229817647 34.0 26.0 35.0 2.0 37.0 82-83 27.190478862144218 34.0 26.0 35.0 2.0 36.5 84-85 26.81638885135831 34.0 25.0 35.0 2.0 36.0 86-87 26.348672142822792 33.5 24.5 35.0 2.0 36.0 88-89 25.858514567842427 33.0 21.5 35.0 2.0 35.0 90-91 25.439349319068153 33.0 19.5 35.0 2.0 35.0 92-93 25.171901227669625 33.0 18.0 35.0 2.0 35.0 94-95 24.797160856552036 33.0 9.5 35.0 2.0 35.0 96-97 24.33187956425858 32.0 2.0 35.0 2.0 35.0 98-99 23.70775806595661 32.0 2.0 34.5 2.0 35.0 100-101 21.208959171569177 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04910854615113891 1101 2 0.07318235608083512 1101 3 0.06762203084482366 1101 4 0.05765268554934977 1101 5 0.050166104951657076 1101 6 0.09857045454903357 1101 7 0.2020280673213719 1101 8 0.16999811550903843 1101 9 0.07477873490842057 1101 10-11 0.04929784758268596 1101 12-13 0.23980685050054262 1101 14-15 0.12445010190999284 1101 16-17 0.05285728777136711 1101 18-19 0.12428770385413657 1101 20-21 0.09142384532000136 1101 22-23 0.09597283058696604 1101 24-25 -0.0032980925563634855 1101 26-27 0.05149609534947075 1101 28-29 0.2053490429186411 1101 30-31 0.1438098261128502 1101 32-33 0.10370240182548685 1101 34-35 -0.0013366586926011337 1101 36-37 0.35448246317202603 1101 38-39 0.11376200662303404 1101 40-41 0.08713878736938341 1101 42-43 -0.1975315412505836 1101 44-45 -0.022571716030306277 1101 46-47 -0.32336733564278575 1101 48-49 -0.11575760153884573 1101 50-51 -0.48688782205581305 1101 52-53 -0.41718808836473187 1101 54-55 -0.2056046278436554 1101 56-57 -0.6331354357492245 1101 58-59 -0.6620055748639793 1101 60-61 -0.7392389236790677 1101 62-63 -0.4760438074232063 1101 64-65 -0.4818192685861682 1101 66-67 -0.7718755438584708 1101 68-69 -1.04795994166804 1101 70-71 -0.8153836484219674 1101 72-73 -0.8326097358057538 1101 74-75 -0.45673084158878297 1101 76-77 -0.560161403354666 1101 78-79 -0.8029387647930477 1101 80-81 -0.580892563684845 1101 82-83 -0.5272052514251584 1101 84-85 -0.5000456821018382 1101 86-87 -0.3391275714194357 1101 88-89 -0.2348694386283654 1101 90-91 -0.24009438171127329 1101 92-93 -0.6749296871590005 1101 94-95 -0.9257540124376362 1101 96-97 -0.9913961607644701 1101 98-99 -0.784634536904278 1101 100-101 -0.46180691071562663 1102 1 0.1439435834406133 1102 2 0.1471315831268214 1102 3 0.1596171412315428 1102 4 0.08290333996728805 1102 5 0.15397778075548274 1102 6 0.07677710244748681 1102 7 -0.009034065340252084 1102 8 -0.12596548403656982 1102 9 -0.22019706397536254 1102 10-11 0.36668746085872783 1102 12-13 0.3628621179475715 1102 14-15 0.3368272442026736 1102 16-17 0.25399511065871394 1102 18-19 0.24710836864397834 1102 20-21 0.27678155263466664 1102 22-23 0.28292645334589395 1102 24-25 0.08025749592587772 1102 26-27 0.08319214908582495 1102 28-29 0.2597490774916622 1102 30-31 0.25291858296572656 1102 32-33 0.48068726378034654 1102 34-35 0.5124338999086788 1102 36-37 0.10450715818857503 1102 38-39 0.18670491174477633 1102 40-41 0.4572430003392043 1102 42-43 0.31233411785940746 1102 44-45 0.4460440048172316 1102 46-47 0.5586151802855 1102 48-49 0.6352765022789981 1102 50-51 0.3958695000165875 1102 52-53 0.25906333159871764 1102 54-55 0.3615719909146762 1102 56-57 0.5162323718272077 1102 58-59 0.47045232013280724 1102 60-61 0.15951768223785834 1102 62-63 0.5147171695116484 1102 64-65 0.30714946752288697 1102 66-67 0.5450201690866585 1102 68-69 0.21737875048388489 1102 70-71 0.2578401625129878 1102 72-73 0.4586587237191182 1102 74-75 0.5508690497531425 1102 76-77 0.5313490673323464 1102 78-79 0.48340303893095893 1102 80-81 0.4212867715253097 1102 82-83 0.5608417431913253 1102 84-85 0.6281801044344384 1102 86-87 0.3544350551425275 1102 88-89 0.3019076147622606 1102 90-91 0.4556259719213642 1102 92-93 0.754507389938837 1102 94-95 0.5024853563577381 1102 96-97 0.6736005054082916 1102 98-99 0.49222746734326606 1102 100-101 0.8672634453865093 1103 1 0.08006113390907643 1103 2 0.19757589878411608 1103 3 0.08974608936252082 1103 4 0.1538731071845234 1103 5 -0.0017379538142066053 1103 6 0.049079816636613316 1103 7 0.23829762595695314 1103 8 0.14788026115805764 1103 9 0.14975608969989906 1103 10-11 0.09765312298564055 1103 12-13 0.3693095244963942 1103 14-15 0.36814666852412614 1103 16-17 0.1863275263836215 1103 18-19 0.09495440360969098 1103 20-21 0.3579114183740657 1103 22-23 0.4290435208625567 1103 24-25 0.3024153245745822 1103 26-27 0.3409918206786031 1103 28-29 0.41655749051204793 1103 30-31 0.4620246391813154 1103 32-33 1.0202470532133603 1103 34-35 0.7514769309141798 1103 36-37 0.9240280700015759 1103 38-39 0.8268937458991346 1103 40-41 0.6159168146925325 1103 42-43 0.2343374019316542 1103 44-45 0.06055791382910769 1103 46-47 0.0583254092046559 1103 48-49 0.27291172878339864 1103 50-51 0.41593011046766293 1103 52-53 0.09136435545654109 1103 54-55 -0.1186873542026774 1103 56-57 -0.09552267501911871 1103 58-59 -0.13952787884333162 1103 60-61 0.10698460003946408 1103 62-63 0.3461851015120345 1103 64-65 0.21065569760112268 1103 66-67 0.4237449348550335 1103 68-69 0.35191101166421745 1103 70-71 0.5168773980968773 1103 72-73 0.6135474033288908 1103 74-75 0.6488237488741717 1103 76-77 0.5335525349262831 1103 78-79 0.40002744731500073 1103 80-81 0.4225786675940846 1103 82-83 0.16710925019413025 1103 84-85 0.13930610655942743 1103 86-87 0.12656028454463097 1103 88-89 0.4581545963135021 1103 90-91 0.5070361911814807 1103 92-93 0.4515577618117277 1103 94-95 0.8739537713099281 1103 96-97 0.5117090246780727 1103 98-99 0.870680572723348 1103 100-101 1.0801425625506198 1104 1 0.19009502273973666 1104 2 0.24026883802878274 1104 3 0.21932719546600765 1104 4 0.03656068943189439 1104 5 0.05150594095573524 1104 6 0.1189478714643144 1104 7 0.04859004813492618 1104 8 0.06821821781296222 1104 9 0.13794158155376834 1104 10-11 0.12782116966139512 1104 12-13 0.34659178002516455 1104 14-15 0.3779202771605341 1104 16-17 0.27124757902194574 1104 18-19 0.3506753273423584 1104 20-21 0.49831715269029075 1104 22-23 0.30792297677795943 1104 24-25 0.189665088750246 1104 26-27 0.39465787012601794 1104 28-29 0.18118733364519102 1104 30-31 0.2446964998062171 1104 32-33 0.16045190013134203 1104 34-35 0.3549019154837012 1104 36-37 0.1466083958467621 1104 38-39 0.05934084555092767 1104 40-41 0.10638776462789679 1104 42-43 0.3708447561572754 1104 44-45 0.2416826503463625 1104 46-47 0.24864438345613848 1104 48-49 -0.33658937173017733 1104 50-51 -0.4819899771075953 1104 52-53 -0.35407288033285766 1104 54-55 -0.3827785861955988 1104 56-57 -0.18460895761236173 1104 58-59 -0.213023552485744 1104 60-61 0.0010383330513725753 1104 62-63 -0.4754006861412492 1104 64-65 -0.4148989263027296 1104 66-67 -0.3916628374492923 1104 68-69 -0.516429134372185 1104 70-71 -0.528846320590894 1104 72-73 -0.6433177051503023 1104 74-75 -0.34516418623628553 1104 76-77 -0.7392409062457368 1104 78-79 -0.5015173476633947 1104 80-81 -0.5153180122321643 1104 82-83 -0.9848664336964497 1104 84-85 -0.8023841425409479 1104 86-87 -0.6257571990641217 1104 88-89 -0.28857006567161747 1104 90-91 -0.33053033457286674 1104 92-93 -0.2479001333541646 1104 94-95 0.02465716834494458 1104 96-97 0.3248051361967832 1104 98-99 0.11187355272777211 1104 100-101 0.5771525428694773 1105 1 -0.07293087125929887 1105 2 -0.0345164859368694 1105 3 0.02611045987048044 1105 4 0.012106168639739678 1105 5 0.21977541128310207 1105 6 -0.1332265711531022 1105 7 0.22174267158402472 1105 8 -0.011733725759491165 1105 9 0.20390290388063192 1105 10-11 0.24119669078019967 1105 12-13 0.034717745444467596 1105 14-15 0.10486232708829135 1105 16-17 -0.08149933302039614 1105 18-19 -0.13847045304862604 1105 20-21 -0.10534465850552266 1105 22-23 -0.11888509094395516 1105 24-25 -0.2174304317041873 1105 26-27 -0.23316226153087172 1105 28-29 0.15188011023263215 1105 30-31 -0.3987724213048338 1105 32-33 -0.5066244641895352 1105 34-35 -0.785549637768689 1105 36-37 -0.6452402817710166 1105 38-39 -0.798560505897683 1105 40-41 -0.7424171916064282 1105 42-43 -0.5375878181281024 1105 44-45 -0.607656573774868 1105 46-47 -0.7771748734409059 1105 48-49 -0.8878126514152598 1105 50-51 -0.9205796206152712 1105 52-53 -0.7565925209062172 1105 54-55 -0.4718933624397792 1105 56-57 -0.6540504954180477 1105 58-59 -0.3206633064456881 1105 60-61 -0.5802478961246358 1105 62-63 -0.7182378977611634 1105 64-65 -0.5489946294601715 1105 66-67 -0.4965288275611819 1105 68-69 0.15147492993927258 1105 70-71 -0.4414727593770813 1105 72-73 -0.5270687639972813 1105 74-75 -0.6787826469911273 1105 76-77 -0.34628272308873775 1105 78-79 -0.2306961253249078 1105 80-81 -0.20338030842142274 1105 82-83 -0.4171998832953321 1105 84-85 -0.41786905293342613 1105 86-87 -0.5673143823373827 1105 88-89 -0.16681512023337675 1105 90-91 0.01115474080596357 1105 92-93 0.1642889255038824 1105 94-95 -0.3248066260089786 1105 96-97 -0.2059886253259684 1105 98-99 -0.3641978896847924 1105 100-101 0.20198298894164068 1106 1 -0.25155552529378866 1106 2 -0.23660516516101282 1106 3 -0.3288081331844417 1106 4 -0.13257184532213984 1106 5 0.0011140036656982488 1106 6 -0.19062603105471254 1106 7 -0.24368318814727274 1106 8 -0.16880486859422206 1106 9 -0.36853415000594936 1106 10-11 -0.4894376446858857 1106 12-13 -0.42682265548333476 1106 14-15 -0.4025948404206545 1106 16-17 -0.41618384609144954 1106 18-19 -0.6192252461416459 1106 20-21 -0.5334902833724655 1106 22-23 -0.45778562095751596 1106 24-25 -0.3395520546886246 1106 26-27 -0.6233469258742943 1106 28-29 -0.6836027847843411 1106 30-31 -0.6262328383683595 1106 32-33 -0.71262941695894 1106 34-35 -0.6652152698070566 1106 36-37 -0.10393171975494653 1106 38-39 -0.18430784742587036 1106 40-41 -0.5394264808504019 1106 42-43 -0.3806483382809489 1106 44-45 -0.20578191693903136 1106 46-47 -0.5918914338434007 1106 48-49 -0.34654050682128457 1106 50-51 -0.25777413447070074 1106 52-53 -0.5939775258925977 1106 54-55 -0.6015225770560519 1106 56-57 -0.7496462284167151 1106 58-59 -0.6676821202462122 1106 60-61 -0.8341613683297382 1106 62-63 -0.764899820825157 1106 64-65 -0.6297348555843492 1106 66-67 -0.6602699613195746 1106 68-69 -0.622574575344057 1106 70-71 -0.14370430694944147 1106 72-73 -0.27441309352452947 1106 74-75 -0.19876740770065027 1106 76-77 -0.34896920830326295 1106 78-79 -0.7336963606374809 1106 80-81 -0.7189585610574838 1106 82-83 -0.28658179560218144 1106 84-85 -0.26197727430653117 1106 86-87 -0.18244105893947093 1106 88-89 -0.24517700856075564 1106 90-91 -0.19595954266724647 1106 92-93 0.11332974663271145 1106 94-95 0.15540190575261903 1106 96-97 0.2528820984282767 1106 98-99 -0.38040364251694925 1106 100-101 -0.33403944419037046 1107 1 -0.22353560001359085 1107 2 -0.3510553628844626 1107 3 -0.30267805081787813 1107 4 -0.17327447362389847 1107 5 -0.1398376906385579 1107 6 -0.31437250808165373 1107 7 -0.38631717036308544 1107 8 -0.1846882882267451 1107 9 -0.1722854335697761 1107 10-11 -0.10381683675073106 1107 12-13 -0.16628104616739847 1107 14-15 -0.322385756065934 1107 16-17 -0.3416770623200662 1107 18-19 -0.25947234583358636 1107 20-21 -0.2976040235135997 1107 22-23 -0.45532227080130383 1107 24-25 -0.49038445948642106 1107 26-27 -0.2843877595899684 1107 28-29 -0.2264683546866877 1107 30-31 -0.40337338587661264 1107 32-33 -0.4757490778796054 1107 34-35 -0.34663472754230185 1107 36-37 -0.3598361133666188 1107 38-39 -0.22596632113192783 1107 40-41 -0.26153218096619213 1107 42-43 -0.29268871319082734 1107 44-45 -0.6062155976538932 1107 46-47 -0.6770818382479433 1107 48-49 -0.6825283593916964 1107 50-51 -0.5542883028305781 1107 52-53 -0.5405102649631957 1107 54-55 -0.4950960187549498 1107 56-57 -0.5977214288711536 1107 58-59 -0.628224821956902 1107 60-61 -0.5917917456428832 1107 62-63 -0.3714465425485187 1107 64-65 -0.4619211744325291 1107 66-67 -0.4947830809804934 1107 68-69 -0.37463373944359546 1107 70-71 -0.3746638664958759 1107 72-73 -0.4049761137775292 1107 74-75 -0.0930672919068094 1107 76-77 -0.45134298209016066 1107 78-79 -0.6016258356325643 1107 80-81 -0.5704201852299953 1107 82-83 -0.40643934732707265 1107 84-85 -0.14980471963217568 1107 86-87 -0.29337920754884905 1107 88-89 -0.2792433387698381 1107 90-91 -0.2580457981565303 1107 92-93 0.13684354357837591 1107 94-95 0.2884610480042582 1107 96-97 0.27387246350660277 1107 98-99 0.5509502715334342 1107 100-101 0.8034875925796356 1108 1 -0.046649320387583515 1108 2 0.006708747325831155 1108 3 -0.004631891714964809 1108 4 0.0830700733272991 1108 5 0.039782102484174686 1108 6 -0.10226117953197189 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-0.36643264536158426 2316 70-71 -0.6079067798283049 2316 72-73 -0.6966145241939117 2316 74-75 -0.8166081391422146 2316 76-77 -0.8412023789887542 2316 78-79 -1.0725399818417074 2316 80-81 -0.9077998136037913 2316 82-83 -1.4149275249119526 2316 84-85 -1.3407943665420703 2316 86-87 -1.8582736421977977 2316 88-89 -1.5875167688525735 2316 90-91 -2.413321805209293 2316 92-93 -2.340777506197835 2316 94-95 -2.0254048591343974 2316 96-97 -1.788171480613837 2316 98-99 -2.5367232218432996 2316 100-101 -3.3303656557588965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 4.0 4 5.0 5 29.0 6 100.0 7 211.0 8 381.0 9 722.0 10 1282.0 11 2063.0 12 2903.0 13 4092.0 14 4518.0 15 5909.0 16 7473.0 17 9835.0 18 8742.0 19 5333.0 20 4420.0 21 4307.0 22 4494.0 23 4731.0 24 5170.0 25 5680.0 26 5796.0 27 6746.0 28 7738.0 29 9053.0 30 10873.0 31 12942.0 32 15442.0 33 19414.0 34 25041.0 35 35258.0 36 49744.0 37 79239.0 38 69338.0 39 7643.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27410196640966 20.895573854153735 15.448522549114266 19.38180163032234 2 15.356289831707814 24.092415287131796 38.22447049447995 22.32682438668044 3 20.91412266620256 27.793973174926318 32.274360459923834 19.017543698947286 4 12.245781245348434 19.21700280067143 37.80288127946945 30.734334674510684 5 11.184136703909719 40.31615756124036 33.831790254213026 14.667915480636895 6 26.5779825547711 38.530643650628264 17.41271088048255 17.478662914118086 7 23.594798033346997 36.98466386217857 21.68264705815 17.73789104632443 8 25.234896869797403 31.889411263599744 20.783821599749018 22.091870266853835 9 24.968798734087354 18.276270320897865 22.818258637311907 33.93667230770288 10-11 23.88975018377259 27.545851548384288 27.038616289694307 21.52578197814881 12-13 27.224106384294256 26.169446346417637 27.360361453784343 19.246085815503765 14-15 22.692220055624194 27.522525049778956 29.3532685796595 20.43198631493735 16-17 18.92788727427202 30.653776703486958 29.62725899734468 20.791077024896346 18-19 18.543941888664214 29.14841336542403 32.91994806253564 19.387696683376117 20-21 20.784050599865804 26.394095460988687 32.458820053998224 20.36303388514728 22-23 19.66801853068945 27.452648500850735 32.905599281855636 19.97373368660418 24-25 20.909657163925154 27.939388249088008 32.63389064328423 18.51706394370261 26-27 20.062173674086285 27.03444169643675 34.54589172849684 18.357492900980123 28-29 18.207570285860847 29.648072620517034 33.223680444077026 18.92067664954509 30-31 19.732482063565854 27.706724130429304 32.82751024203022 19.73328356397462 32-33 18.977468678509027 28.543032556946603 33.27394596971244 19.205552794831927 34-35 20.265041560889365 27.918909603491137 33.03377888622084 18.782269949398657 36-37 19.50324842274274 27.55564920928134 33.88838435489062 19.05271801308531 38-39 19.00779504538807 27.945930532827486 33.67695508830835 19.369319333476096 40-41 19.0395271264481 28.303708122876586 33.12702751291368 19.529737237761637 42-43 18.922310786625186 28.22733659066108 32.00467644475692 20.845676177956815 44-45 19.17310365517131 27.498746189905855 32.148148826685656 21.18000132823718 46-47 20.635582206721086 28.293644750446845 31.186214777503864 19.884558265328206 48-49 20.04140322111564 27.997210778577497 31.170121896762165 20.791264103544695 50-51 18.14187815951131 28.825849740369037 32.041380652770066 20.99089144734959 52-53 19.257650321401666 29.654828123962346 30.215992910156388 20.87152864447961 54-55 18.524620947556684 28.313459939864572 30.4828925462752 22.67902656630355 56-57 19.922552160501603 28.328001447280737 29.59677659435606 22.152669797861595 58-59 20.37869922757907 28.277750933752255 30.060410507489483 21.283139331179196 60-61 19.40796328653313 29.313637706415925 30.53467192148008 20.743727085570864 62-63 18.38344237555561 29.469796029595972 30.377323492434982 21.76943810241343 64-65 18.05791870953854 30.480488045048904 29.464414526851407 21.99717871856115 66-67 18.82300269883127 29.247911182459085 28.555741686237106 23.37334443247254 68-69 18.533013493940036 28.98580760400982 28.97836504056197 23.50281386148817 70-71 19.702408555610898 28.899919275887537 28.387636043029957 23.010036125471604 72-73 19.243566814219076 27.997096278519102 29.552121571582003 23.20721533567982 74-75 18.303635834854276 30.03611331841779 28.033392797030327 23.626858049697606 76-77 18.61874045854021 28.565468740687766 28.42128668161209 24.394504119159937 78-79 18.881805311842832 28.666953776796312 28.50511071302579 23.946130198335066 80-81 19.35728753631087 28.535966227024108 27.873468130589757 24.233278106075264 82-83 18.79403958496019 28.332123449382955 28.61161809192523 24.262218873731626 84-85 18.58118397640383 28.644823108859786 28.0778188196876 24.69617409504879 86-87 18.5239293843781 28.824544604922913 27.710496591345628 24.94102941935336 88-89 18.53007362037891 28.68104766313594 26.96982471940056 25.81905399708459 90-91 18.93026417312709 27.90845335635816 27.296588529228057 25.8646939412867 92-93 19.29738184166474 28.324451945470493 27.3358584412878 25.042307771576965 94-95 18.804022620657438 27.79572250192074 27.626033221084985 25.77422165633684 96-97 18.059542388542653 27.9237337137793 28.162237608503244 25.85448628917481 98-99 18.5807472511367 28.2429602420992 26.961245120860678 26.21504738590342 100-101 18.440110974091922 27.529292145136523 27.6727615396682 26.357835341103353 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1032.0 1 701.5 2 405.0 3 417.5 4 363.5 5 386.5 6 590.5 7 989.0 8 1300.5 9 1388.0 10 1281.5 11 1126.0 12 1198.5 13 1323.5 14 1379.5 15 1470.5 16 1620.5 17 1764.5 18 1879.5 19 2027.5 20 2176.0 21 2434.0 22 2770.5 23 3134.5 24 3465.5 25 3976.0 26 4637.5 27 5238.0 28 5745.0 29 6302.5 30 7030.0 31 7670.0 32 8244.0 33 8917.5 34 9709.0 35 10239.5 36 10658.5 37 11274.5 38 11384.0 39 11050.5 40 10905.5 41 11194.5 42 11733.5 43 12352.5 44 12958.0 45 13315.0 46 14328.5 47 18484.5 48 24828.5 49 25923.5 50 21132.5 51 16055.0 52 12209.0 53 10133.0 54 9232.0 55 8350.0 56 7325.0 57 6344.5 58 5796.0 59 5241.0 60 4483.5 61 3747.0 62 3102.5 63 2358.0 64 1653.0 65 1263.5 66 941.0 67 723.0 68 572.0 69 395.5 70 292.0 71 248.0 72 228.5 73 194.0 74 135.0 75 109.0 76 81.0 77 47.0 78 29.0 79 15.5 80 8.0 81 5.5 82 5.0 83 4.5 84 2.5 85 3.0 86 2.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3835751956233498 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1450005839502978E-4 16-17 0.0024045012262956253 18-19 4.5800023358011913E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.2900011679005957E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007442503795676935 36-37 0.019350509868760035 38-39 0.013167506715428424 40-41 0.007213503678886876 42-43 0.005610502861356459 44-45 0.002748001401480715 46-47 0.0024045012262956253 48-49 0.0 50-51 8.015004087652086E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.5800023358011913E-4 60-61 4.5800023358011913E-4 62-63 0.0 64-65 0.0 66-67 0.0024045012262956253 68-69 8.015004087652086E-4 70-71 0.0019465009927155062 72-73 0.0 74-75 0.0 76-77 0.09778304986935545 78-79 0.033663017168138756 80-81 1.1450005839502978E-4 82-83 0.0 84-85 0.0 86-87 0.15079657690625423 88-89 0.3223176643820088 90-91 0.27686114119918204 92-93 0.0 94-95 1.1450005839502978E-4 96-97 1.1450005839502978E-4 98-99 1.1450005839502978E-4 100-101 5.725002919751489E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 436681.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.19963100294246 #Duplication Level Percentage of deduplicated Percentage of total 1 87.16323939739206 55.08684566936316 2 7.972759589788185 10.077509282995685 3 1.9511551252026507 3.6993685182692246 4 0.8341814434148205 2.108798376532743 5 0.4646094794793616 1.4681573831782413 6 0.3062468746880937 1.161281368565513 7 0.21640254496113745 0.9573592692749088 8 0.15619796143922587 0.7897322821096727 9 0.12323271822716833 0.7009436087501958 >10 0.7341602476680352 8.560777492591837 >50 0.04596491374014819 2.0208953514409895 >100 0.020991850363025637 2.7320400760540973 >500 0.0047050699089540215 2.189246112517897 >1k 0.005790855272558796 7.240683908865744 >5k 3.6192845453492475E-4 1.2063612994900628 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5274 1.2077466159507741 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4334 0.9924865061681181 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3885 0.8896654537293814 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2959 0.6776113455817863 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2640 0.6045603083257572 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2505 0.5736452925590992 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1803 0.4128872105724774 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1587 0.3634231853458245 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1551 0.35517918114138236 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1444 0.330676168644846 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1439 0.3295311680608957 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1411 0.32311916479077407 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1350 0.3091501576665804 RNA PCR Primer, Index 17 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1325 0.30342515474682896 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1256 0.28762414668831476 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1133 0.2594571323231375 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1033 0.23655712064413154 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 0.21617611024981623 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 917 0.2099931070964846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 851 0.1948790993883407 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 837 0.19167309775327987 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 791 0.18113909238093712 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 781 0.17884909121303652 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 753 0.17243708794291485 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 740 0.16946008642464408 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 692 0.1584680808187212 RNA PCR Primer, Index 17 (95% over 23bp) GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 636 0.1456440742784779 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 564 0.12915606586959358 RNA PCR Primer, Index 17 (96% over 28bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 564 0.12915606586959358 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 501 0.11472905851181985 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 498 0.11404205816144966 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 492 0.11266805746070932 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 442 0.10121805162120633 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 441 0.10098905150441627 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.2900011679005957E-4 0.0 20-21 0.0 0.0 0.0 0.0011450005839502978 0.0 22-23 0.0 0.0 0.0 0.004007502043826042 0.0 24-25 0.0 0.0 0.0 0.009618004905182501 0.0 26-27 0.0 0.0 0.0 0.019236009810365003 0.0 28-29 0.0 0.0 0.0 0.052670026861713695 0.0 30-31 0.0 0.0 0.0 0.14094957188428167 0.0 32-33 0.0 0.0 0.0 0.311440158834481 0.0 34-35 0.0 0.0 0.0 0.5744467929678645 0.0 36-37 0.0 0.0 0.0 0.9180614682113488 0.0 38-39 0.0 0.0 0.0 1.396213712068993 0.0 40-41 0.0 0.0 0.0 1.930470984540202 0.0 42-43 0.0 0.0 0.0 2.435759742237469 0.0 44-45 0.0 0.0 0.0 3.0245190425047115 0.0 46-47 0.0 0.0 0.0 3.6654903694000884 0.0 48-49 0.0 0.0 0.0 4.275088680295227 0.0 50-51 0.0 0.0 0.0 4.919037008708875 0.0 52-53 0.0 0.0 0.0 5.534818322757344 0.0 54-55 0.0 0.0 0.0 6.185636654674694 0.0 56-57 0.0 0.0 0.0 6.982442561045707 0.0 58-59 0.0 0.0 0.0 7.743638949255864 0.0 60-61 0.0 0.0 0.0 8.57490937320378 0.0 62-63 0.0 0.0 0.0 9.393584790728243 0.0 64-65 0.0 0.0 0.0 10.24672472582961 0.0 66-67 0.0 0.0 0.0 11.164328193807378 0.0 68-69 0.0 0.0 0.0 12.18189021276401 0.0 70-71 0.0 0.0 0.0 13.256931261034943 0.0 72-73 0.0 0.0 0.0 14.238654761713928 0.0 74-75 0.0 0.0 0.0 15.292742299298574 0.0 76-77 0.0 0.0 0.0 16.339959833379517 0.0 78-79 0.0 0.0 0.0 17.581483966556824 0.0 80-81 0.0 0.0 0.0 18.831481104055364 0.0 82-83 0.0 0.0 0.0 20.01598420815195 0.0 84-85 0.0 0.0 0.0 21.203807813941985 0.0 86-87 0.0 0.0 0.0 22.380524914067706 0.0 88-89 0.0 0.0 0.0 23.612087542164645 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCCGC 45 9.662848E-4 42.18278 9 GTGCTAT 60 0.003882605 31.77535 1 ATGGGCG 85 6.435843E-4 27.915075 5 AGTCGGT 35 0.008323414 27.187548 80-81 TACCTGG 320 0.0 23.727816 2 TTGGACT 100 0.0016610201 23.727814 4 GAGTACT 4155 0.0 22.557127 12-13 GTTATAG 215 4.2486135E-8 22.168848 1 CATGGGG 1070 0.0 21.73202 4 GTACATG 3180 0.0 21.583258 1 TGCTATA 110 0.002890171 21.57074 2 TACATGG 3195 0.0 21.239922 2 AGTACTT 4265 0.0 21.140842 12-13 ATGGGAT 485 0.0 20.547798 5 GTACTTT 4580 0.0 20.412136 14-15 ATGGGGA 520 0.0 20.077381 5 ACATGGG 3100 0.0 20.05383 3 ATAGGAT 190 8.298779E-6 19.981316 6 TAGAACC 145 6.1764213E-4 19.636812 4 ATGTAGA 245 1.6877493E-7 19.454296 1 >>END_MODULE