##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938824_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 434874 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.931058191568134 31.0 31.0 34.0 30.0 34.0 2 32.11627045994932 33.0 31.0 34.0 30.0 34.0 3 32.1713944728818 34.0 31.0 34.0 30.0 34.0 4 35.82763973012873 37.0 35.0 37.0 35.0 37.0 5 35.74735900513712 37.0 35.0 37.0 33.0 37.0 6 35.81254340337661 37.0 35.0 37.0 33.0 37.0 7 35.72102953959078 37.0 35.0 37.0 33.0 37.0 8 35.740292590497475 37.0 35.0 37.0 33.0 37.0 9 37.30967820564118 39.0 37.0 39.0 34.0 39.0 10-11 37.257672337274705 39.0 37.0 39.0 33.0 39.0 12-13 37.19644540717541 39.0 37.0 39.0 33.0 39.0 14-15 38.27271347562743 40.0 38.0 41.0 33.0 41.0 16-17 38.24145039712653 40.0 37.5 41.0 33.0 41.0 18-19 38.45202978333954 40.0 38.0 41.0 33.5 41.0 20-21 38.46958429338153 40.0 38.0 41.0 34.0 41.0 22-23 38.36600716529386 40.0 38.0 41.0 34.0 41.0 24-25 38.23627073589132 40.0 37.5 41.0 33.0 41.0 26-27 37.97944577049904 40.0 37.0 41.0 33.0 41.0 28-29 37.76591495467653 40.0 37.0 41.0 33.0 41.0 30-31 37.419418038328345 39.5 36.0 41.0 32.5 41.0 32-33 37.036138053781094 39.0 35.0 41.0 31.5 41.0 34-35 36.77457953338208 39.0 35.0 41.0 31.0 41.0 36-37 36.51001439497418 39.0 35.0 41.0 30.5 41.0 38-39 36.228517915534155 39.0 35.0 41.0 30.0 41.0 40-41 35.94747674038917 39.0 35.0 41.0 30.0 41.0 42-43 35.548089791525825 39.0 35.0 41.0 28.0 41.0 44-45 35.04071984068949 39.0 35.0 41.0 25.0 41.0 46-47 34.35170072250813 38.5 34.5 41.0 21.0 41.0 48-49 33.72265414809807 38.0 34.0 40.0 10.0 41.0 50-51 33.30802255365922 38.0 33.0 40.0 2.0 41.0 52-53 33.05770177108772 38.0 33.0 40.0 2.0 41.0 54-55 32.747949980914015 38.0 33.0 40.0 2.0 41.0 56-57 32.36888271085418 38.0 32.5 40.0 2.0 41.0 58-59 31.980945055349366 37.0 32.0 40.0 2.0 41.0 60-61 31.489730358678607 36.5 31.0 40.0 2.0 41.0 62-63 31.093770839369565 36.0 31.0 39.5 2.0 41.0 64-65 30.63502991671151 36.0 30.5 39.0 2.0 40.5 66-67 30.11511495283691 35.0 29.5 39.0 2.0 40.0 68-69 29.740531740228207 35.0 29.0 38.0 2.0 40.0 70-71 29.272472026380058 35.0 29.0 37.5 2.0 40.0 72-73 28.79684345350607 35.0 28.0 37.0 2.0 39.0 74-75 28.33357363282238 34.0 27.5 36.5 2.0 39.0 76-77 26.514906616629183 32.0 25.0 34.5 2.0 36.5 78-79 27.317994407575526 34.0 26.0 35.0 2.0 37.0 80-81 27.293096391138583 34.0 26.0 35.0 2.0 37.0 82-83 26.94589467293975 34.0 25.5 35.0 2.0 36.5 84-85 26.55881013810897 34.0 24.5 35.0 2.0 36.0 86-87 26.05735569383316 33.0 22.5 35.0 2.0 36.0 88-89 25.492151749702213 33.0 19.0 35.0 2.0 35.0 90-91 24.9753422830521 32.5 16.5 35.0 2.0 35.0 92-93 24.709804449104798 32.0 9.5 35.0 2.0 35.0 94-95 24.370235976397762 32.0 3.5 35.0 2.0 35.0 96-97 23.823990397218502 32.0 2.0 34.0 2.0 35.0 98-99 23.133954892681558 32.0 2.0 34.0 2.0 35.0 100-101 20.22007294066787 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0644224520669141 1101 2 0.07737713104933874 1101 3 -0.2607152288877863 1101 4 0.11944157239685893 1101 5 -0.2501502534853728 1101 6 0.09918198508241005 1101 7 0.002117562463581635 1101 8 0.02896943502516791 1101 9 0.18715445902922312 1101 10-11 -0.1553212080521007 1101 12-13 -0.03855377604514132 1101 14-15 -0.02670864702374587 1101 16-17 0.06923909563980857 1101 18-19 0.03542976911325724 1101 20-21 -0.24512885047198552 1101 22-23 -0.2710833666778498 1101 24-25 -0.21564114282683278 1101 26-27 -0.0580603920863112 1101 28-29 0.0700224274709953 1101 30-31 0.045598261802943796 1101 32-33 0.09147011723604948 1101 34-35 0.41366297742552405 1101 36-37 0.46464621206145296 1101 38-39 0.498575069854752 1101 40-41 0.6567782300798228 1101 42-43 0.7012451173496643 1101 44-45 0.5621918217266781 1101 46-47 0.2867976931031819 1101 48-49 -0.09136783437938334 1101 50-51 -0.12179138730426331 1101 52-53 -0.16472698712589562 1101 54-55 0.26789642732134666 1101 56-57 0.1246024938690411 1101 58-59 -0.29792145796118064 1101 60-61 -0.27509009970725984 1101 62-63 -0.3103657378770457 1101 64-65 -0.22910444185126622 1101 66-67 -0.318103646939516 1101 68-69 -0.5179960497268645 1101 70-71 -0.4177893084455597 1101 72-73 -0.6048105370304384 1101 74-75 -0.635337208294775 1101 76-77 -0.6503771337740005 1101 78-79 -0.40861075544161096 1101 80-81 -0.6504681348075074 1101 82-83 -0.6291569648818616 1101 84-85 -0.4083473094270893 1101 86-87 -0.3475079287042888 1101 88-89 -0.25502418886570055 1101 90-91 -0.2085585150848459 1101 92-93 -0.30219390447794225 1101 94-95 -0.4268893124872619 1101 96-97 -0.5808650202833725 1101 98-99 -0.4070478164485003 1101 100-101 -0.0961147354770091 1102 1 0.19360748761770097 1102 2 0.33086524964537034 1102 3 0.06217191594180349 1102 4 0.09749549289682591 1102 5 0.01723583764172787 1102 6 -0.035964443229481446 1102 7 0.11733447983877454 1102 8 0.03549149010837027 1102 9 0.3529309287625253 1102 10-11 0.1856696356365859 1102 12-13 0.3580743759786884 1102 14-15 0.38363580468760716 1102 16-17 0.5375644353680258 1102 18-19 0.48078257777542177 1102 20-21 0.3803071643525229 1102 22-23 0.24712025224518896 1102 24-25 0.14765912604898546 1102 26-27 0.10049458211614137 1102 28-29 -0.012139116021479879 1102 30-31 0.3283992937046989 1102 32-33 0.4296722827763446 1102 34-35 0.36858994494242125 1102 36-37 0.3984991297273197 1102 38-39 0.664960143613925 1102 40-41 0.4087221295057333 1102 42-43 0.39154742096132367 1102 44-45 0.3370719176499364 1102 46-47 0.22749967697063056 1102 48-49 0.46866595676952016 1102 50-51 0.5281432104720594 1102 52-53 0.5433792551530061 1102 54-55 0.37877136373585074 1102 56-57 0.7038027700117695 1102 58-59 0.6222969127967559 1102 60-61 0.5301384447299178 1102 62-63 0.7036049037914367 1102 64-65 0.729846579150113 1102 66-67 0.7026161408666063 1102 68-69 0.4114967196939645 1102 70-71 0.4168702366456394 1102 72-73 0.375187282895439 1102 74-75 0.5423206654062689 1102 76-77 0.3480423124871663 1102 78-79 0.41928147622759937 1102 80-81 0.7930861127035733 1102 82-83 0.6059549455897617 1102 84-85 0.6738505686340979 1102 86-87 0.7286129927403735 1102 88-89 0.469069263645018 1102 90-91 0.51905030694844 1102 92-93 0.7100417615347041 1102 94-95 0.9145647969524582 1102 96-97 1.018074010310336 1102 98-99 1.3629725309099783 1102 100-101 1.6978591763026571 1103 1 0.2924879985935398 1103 2 0.298312445734652 1103 3 0.18775990708734724 1103 4 0.042057151262440584 1103 5 -0.11072717656229258 1103 6 0.009195582596007057 1103 7 0.003938658034677189 1103 8 0.19362075592648864 1103 9 0.21755461442937474 1103 10-11 0.17112838402249508 1103 12-13 0.1527098214684557 1103 14-15 0.08962293805783617 1103 16-17 0.1199869588876723 1103 18-19 -0.10951729770881258 1103 20-21 0.04100168565855711 1103 22-23 -0.12280976527924992 1103 24-25 0.1305612658755777 1103 26-27 0.09468096378004276 1103 28-29 0.023293115116686636 1103 30-31 0.09219402714870739 1103 32-33 -0.027107485716555857 1103 34-35 0.07690598441853069 1103 36-37 0.2698011265913678 1103 38-39 0.40272473962942 1103 40-41 0.27619353699512317 1103 42-43 0.2868244685540162 1103 44-45 0.28975592429078745 1103 46-47 0.36703516396565306 1103 48-49 0.49553484627329425 1103 50-51 0.4712665963787188 1103 52-53 0.44506079453687875 1103 54-55 0.3910874723873903 1103 56-57 -0.03759641832986915 1103 58-59 0.11418032914060205 1103 60-61 0.18405665631449608 1103 62-63 0.050441857305550286 1103 64-65 0.2511670227951974 1103 66-67 0.025452102866236004 1103 68-69 -0.4283131631689763 1103 70-71 -0.37359738925364283 1103 72-73 -0.3432283691982718 1103 74-75 -0.47975494333750746 1103 76-77 -0.4552285323754006 1103 78-79 -0.39634871192956567 1103 80-81 -0.41841199650137284 1103 82-83 -0.2919507683006657 1103 84-85 -0.26565840111318195 1103 86-87 -0.0653595215558802 1103 88-89 -0.1345526465191611 1103 90-91 0.29416098763465826 1103 92-93 0.463321829787791 1103 94-95 0.2107855126875613 1103 96-97 0.04764362385027354 1103 98-99 0.21148122395554125 1103 100-101 0.9296112746240013 1104 1 0.1009815883371239 1104 2 -0.05540906423685499 1104 3 0.03747500680244542 1104 4 0.020956723912014752 1104 5 0.0368867835516653 1104 6 0.20524259114301913 1104 7 0.09092227626829441 1104 8 0.1564946875504205 1104 9 0.04447769135245494 1104 10-11 0.10172845524756724 1104 12-13 -0.06790983665120365 1104 14-15 -0.06974315880825799 1104 16-17 0.02872983638055615 1104 18-19 -0.03317292448943476 1104 20-21 -0.14453962488275351 1104 22-23 0.11138717204269 1104 24-25 0.049720810035118745 1104 26-27 -0.04122537525130099 1104 28-29 -0.11768801023944064 1104 30-31 0.03539203284670833 1104 32-33 -0.2860551352892102 1104 34-35 -0.28856766230511255 1104 36-37 -0.266835269420028 1104 38-39 -0.17335253957285346 1104 40-41 0.20844068799227955 1104 42-43 -0.1883803035257543 1104 44-45 -0.27577114123628377 1104 46-47 -0.7382443944389081 1104 48-49 -1.0479124471739922 1104 50-51 -1.0808612526241248 1104 52-53 -1.2529003877993006 1104 54-55 -1.4831557612308437 1104 56-57 -1.5164959909794398 1104 58-59 -1.068333915873641 1104 60-61 -0.8933560929732529 1104 62-63 -0.7970029432642498 1104 64-65 -1.1383005697973942 1104 66-67 -1.3066347917206578 1104 68-69 -1.1176383056191206 1104 70-71 -1.0263751670314214 1104 72-73 -1.0648273720472723 1104 74-75 -1.0149668379244012 1104 76-77 -1.0607306691275369 1104 78-79 -1.1732361763170296 1104 80-81 -0.952867124706497 1104 82-83 -0.7958236315485294 1104 84-85 -0.45916801649779515 1104 86-87 -0.49039513409149293 1104 88-89 -1.0411142562057698 1104 90-91 -1.204770636296967 1104 92-93 -1.2528462613802986 1104 94-95 -0.8039936895916391 1104 96-97 -0.9530241111924624 1104 98-99 -0.7971993316000159 1104 100-101 -0.08138409574636896 1105 1 0.11867565226406995 1105 2 0.08059423358150752 1105 3 0.18058129802517442 1105 4 -0.08216351961919344 1105 5 0.016973034185859603 1105 6 -0.06682742407767961 1105 7 -0.11411323869872803 1105 8 -0.138977154154297 1105 9 -0.1615725369580474 1105 10-11 -0.002612615275012331 1105 12-13 0.16073361844772904 1105 14-15 0.12449517198829341 1105 16-17 -0.06299073693248403 1105 18-19 -0.001272873753769943 1105 20-21 0.062208218851395714 1105 22-23 -0.028659124253607615 1105 24-25 0.14050594448463727 1105 26-27 0.11370866838137772 1105 28-29 0.21494770767024818 1105 30-31 0.13773538647431138 1105 32-33 0.15325232285386647 1105 34-35 -0.09796010269070621 1105 36-37 0.2918912653339021 1105 38-39 0.16013934272039876 1105 40-41 0.16618739017390993 1105 42-43 0.5750170835873831 1105 44-45 0.3435845157906101 1105 46-47 0.517495077910155 1105 48-49 0.56632207844752 1105 50-51 0.9285407006990596 1105 52-53 0.6847264513686397 1105 54-55 0.836320393005586 1105 56-57 1.1954998233280136 1105 58-59 1.2727683164957142 1105 60-61 1.0264181333078248 1105 62-63 1.1192039314186495 1105 64-65 0.9598570912883488 1105 66-67 1.0877874558693996 1105 68-69 1.0817687212707874 1105 70-71 1.1909003276413337 1105 72-73 1.3491122525087853 1105 74-75 1.541983948478439 1105 76-77 1.522412405453892 1105 78-79 0.8801155486956524 1105 80-81 1.524016436937334 1105 82-83 1.8196634308563446 1105 84-85 1.810951846515902 1105 86-87 1.8545122733159118 1105 88-89 1.49701676865471 1105 90-91 1.3869340821200815 1105 92-93 1.495076747244763 1105 94-95 1.6410849506924805 1105 96-97 1.7102654626279339 1105 98-99 1.6287900139098817 1105 100-101 2.3962922106970623 1106 1 0.2610887575906382 1106 2 0.26529287384312994 1106 3 0.1035986415236465 1106 4 -0.07075305682216282 1106 5 0.22989409241262848 1106 6 0.08433842755592735 1106 7 0.19686996453305738 1106 8 -0.014575773154184901 1106 9 0.2193668266074411 1106 10-11 0.017535406877684068 1106 12-13 0.15890865312460534 1106 14-15 -0.02984981479905713 1106 16-17 0.09569265562995355 1106 18-19 0.04996536901470705 1106 20-21 0.08238408374826633 1106 22-23 0.008295790750089793 1106 24-25 0.2969543240369248 1106 26-27 0.16197533099818884 1106 28-29 0.08951614513544826 1106 30-31 0.1230496044981848 1106 32-33 0.04539965653143696 1106 34-35 0.14814191312397185 1106 36-37 -0.04428455906444384 1106 38-39 0.3380367599261973 1106 40-41 0.1825234875323929 1106 42-43 0.25119584412966134 1106 44-45 0.30766484463872956 1106 46-47 0.4357260005680672 1106 48-49 0.2379928455910516 1106 50-51 -0.0040899830174865315 1106 52-53 -0.2756533236619916 1106 54-55 0.20205508708976083 1106 56-57 -0.001629336631076228 1106 58-59 -0.22071648136596167 1106 60-61 -0.15837415982058545 1106 62-63 0.18218168759720754 1106 64-65 0.071185038316262 1106 66-67 0.33411980711320766 1106 68-69 0.4154235117414764 1106 70-71 0.39441813181065655 1106 72-73 0.10971274456601421 1106 74-75 0.4131353218850755 1106 76-77 0.11403528307743827 1106 78-79 0.10503176734725272 1106 80-81 0.1610002071486356 1106 82-83 0.14360110360791367 1106 84-85 0.05725382385697131 1106 86-87 -0.07859933052739976 1106 88-89 -0.2024866180354472 1106 90-91 -0.34162700486065134 1106 92-93 -0.06355754084157894 1106 94-95 0.012689401481697615 1106 96-97 0.30802386860552033 1106 98-99 0.47093937251429807 1106 100-101 0.864374301231539 1107 1 -0.07995448472223288 1107 2 -0.13173453354177767 1107 3 -0.2235282910159171 1107 4 -0.10042835980183895 1107 5 -0.22960230827989392 1107 6 -0.39787765238818196 1107 7 -0.1483598140411928 1107 8 -0.15952509935977588 1107 9 -0.38303372417266246 1107 10-11 -0.5048957202978457 1107 12-13 -0.19999697515957848 1107 14-15 -0.22481989650486156 1107 16-17 -0.3986071752886531 1107 18-19 -0.23871579612819716 1107 20-21 -0.4172438359431254 1107 22-23 -0.4236362932033799 1107 24-25 -0.6026447404468698 1107 26-27 -0.5540995507967068 1107 28-29 -0.5122212421014396 1107 30-31 -0.5905751158087895 1107 32-33 -0.8284828369634809 1107 34-35 -0.6036678652477789 1107 36-37 -0.7845927529309407 1107 38-39 -0.6492213878732116 1107 40-41 -0.5689724271776839 1107 42-43 -0.7469007246317929 1107 44-45 -0.2534474476797115 1107 46-47 -0.4003131412679295 1107 48-49 -0.11290166584472416 1107 50-51 -0.22100267829637943 1107 52-53 0.05230636004443312 1107 54-55 0.4755897993982785 1107 56-57 0.47289708360198546 1107 58-59 0.6255080425231156 1107 60-61 0.6702537497461769 1107 62-63 1.0404368081309805 1107 64-65 0.9130534383203113 1107 66-67 1.0101618850931438 1107 68-69 0.665102054604116 1107 70-71 0.3324528390568595 1107 72-73 0.27605289177819614 1107 74-75 0.3033994735925951 1107 76-77 0.08405624107033205 1107 78-79 0.13924796878697876 1107 80-81 0.11191598031632921 1107 82-83 0.11875018884720845 1107 84-85 0.07921668669855109 1107 86-87 0.4595351043661431 1107 88-89 0.6191628873761701 1107 90-91 0.6780299725310392 1107 92-93 0.8101452403954497 1107 94-95 0.5657424849390544 1107 96-97 0.5013613530388916 1107 98-99 0.4552740321747173 1107 100-101 1.1554246307883815 1108 1 0.17979684938130092 1108 2 0.27283167650388407 1108 3 0.24166398493721175 1108 4 0.09478537183704816 1108 5 -0.0634032655335659 1108 6 -0.08277615049018294 1108 7 0.10927345877610861 1108 8 0.036849439893124725 1108 9 0.30080299255727283 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-0.4156001394041233 2316 70-71 -0.7506299095788478 2316 72-73 -0.8756900640075251 2316 74-75 -1.1187382923056113 2316 76-77 -0.9536845986668325 2316 78-79 -0.7795765105537065 2316 80-81 -0.9716679377146313 2316 82-83 -0.7240079133913468 2316 84-85 -0.6326944392620213 2316 86-87 -0.5631706535133567 2316 88-89 -1.3083624495210344 2316 90-91 -1.6576839154297573 2316 92-93 -1.6453202902872555 2316 94-95 -1.7695537618590294 2316 96-97 -1.8143892873442233 2316 98-99 -1.8890014996216955 2316 100-101 -1.8177210424491719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 5.0 5 34.0 6 76.0 7 197.0 8 370.0 9 723.0 10 1367.0 11 2047.0 12 3070.0 13 4135.0 14 4682.0 15 6049.0 16 7472.0 17 10005.0 18 9080.0 19 5322.0 20 4547.0 21 4497.0 22 4607.0 23 4750.0 24 5141.0 25 5661.0 26 5989.0 27 6708.0 28 7916.0 29 9424.0 30 10824.0 31 13285.0 32 16039.0 33 19790.0 34 25702.0 35 35345.0 36 50676.0 37 78423.0 38 64660.0 39 6251.0 >>END_MODULE >>Per base sequence content fail #Base G 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18-19 18.575840358356675 29.142924157342126 32.90516333466705 19.376072149634147 20-21 20.808785993184234 26.34257279119929 32.60772085707585 20.240920358540635 22-23 19.691335881197773 27.488651885373695 32.85733338852173 19.9626788449068 24-25 20.83964743729779 27.87813913487386 32.67425202300442 18.607961404823936 26-27 19.993906224848807 26.949778094602983 34.6994504104675 18.356865270080714 28-29 18.279087735757944 29.591099950790344 33.26664735072688 18.863164962724834 30-31 19.64971462998478 27.665484715112882 32.92735367025851 19.757446984643828 32-33 18.884665443323815 28.539301038921614 33.38403767528065 19.19199584247391 34-35 20.37071106613927 27.9309631128691 32.9375172477233 18.760808573268328 36-37 19.49485633855941 27.56866839770289 33.871464810824115 19.06501045291359 38-39 19.05883326970576 27.94361830818128 33.64089213517255 19.356656286940403 40-41 19.035238815821245 28.274417994954387 33.20044706818479 19.48989612103957 42-43 18.906926257594783 28.326641185912916 31.99064939126755 20.77578316522475 44-45 19.261919983074936 27.53151603511951 32.03643488219143 21.170129099614126 46-47 20.697239647528466 28.307754925587297 31.198720543056346 19.796284883827887 48-49 20.16200336884223 27.93057655492765 31.08717023001259 20.820249846217525 50-51 18.109519205010194 28.866095542726793 32.04299641160016 20.98138884066286 52-53 19.387684708674236 29.689174335554668 29.99294048391028 20.930200471860815 54-55 18.50202587416125 28.357639224235065 30.47790854362413 22.662426357979555 56-57 19.82070441984186 28.376394243317232 29.681961328539547 22.12094000830136 58-59 20.434241721146172 28.25319116155751 29.87780340467217 21.434763712624143 60-61 19.471374958607747 29.29847032635491 30.60830512528055 20.621849589756795 62-63 18.418816636216988 29.453595955577576 30.38686229578691 21.740725112418524 64-65 18.112737658323905 30.42107030179273 29.54643707975754 21.919754960125832 66-67 18.877379133192367 29.195915359027342 28.558681931753245 23.368023576027053 68-69 18.513451053525973 28.992832390146088 29.11286743486029 23.380849121467648 70-71 19.63341063550665 28.89851876465225 28.38825543520127 23.079815164639832 72-73 19.291219985559035 27.981898204997307 29.57040430101593 23.156477508427727 74-75 18.42643533449459 30.026846949393217 28.003035372436745 23.543682343675446 76-77 18.613651345496546 28.467971188392525 28.53147545019575 24.38690201591518 78-79 18.834437970506936 28.65897392503664 28.46487092611077 24.041717178345657 80-81 19.366388270571573 28.632218215934802 27.80093912691551 24.20045438657812 82-83 18.828522119854345 28.223064620535588 28.612606770536907 24.335806489073157 84-85 18.577450020005795 28.61587494308696 27.975114630904585 24.83156040600266 86-87 18.63166314674892 28.7422096643322 27.6337520788615 24.992375110057374 88-89 18.509977018708064 28.52114463446825 27.033475158977012 25.935403187846678 90-91 18.96850201046086 27.854000378548317 27.211045301153646 25.96645230983718 92-93 19.28917259846829 28.334561659885 27.248383725382208 25.1278820162645 94-95 18.916464021063646 27.769978556933356 27.472075148463055 25.841482273539945 96-97 18.122626464937504 27.91489939028246 28.099665764871855 25.86280837990818 98-99 18.63160363691552 28.277156141779 26.764993998261566 26.326246223043913 100-101 18.56338510748627 27.436736768156884 27.624379267803327 26.37549885655352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1019.0 1 681.5 2 381.5 3 388.5 4 363.5 5 387.0 6 555.0 7 907.5 8 1297.5 9 1425.5 10 1260.0 11 1141.0 12 1191.0 13 1278.0 14 1333.0 15 1440.5 16 1591.0 17 1725.5 18 1847.0 19 1978.0 20 2156.5 21 2376.5 22 2690.0 23 3076.0 24 3471.5 25 3922.0 26 4585.0 27 5350.5 28 5853.0 29 6319.0 30 7002.0 31 7602.5 32 8189.0 33 8897.0 34 9616.0 35 10216.5 36 10688.5 37 11244.5 38 11360.5 39 10989.0 40 10965.5 41 11264.0 42 11709.0 43 12248.5 44 12601.5 45 13091.5 46 14436.0 47 18623.5 48 24856.5 49 25693.0 50 20837.5 51 16059.0 52 12216.5 53 10090.0 54 9307.0 55 8351.5 56 7267.5 57 6408.5 58 5856.5 59 5190.0 60 4439.5 61 3733.5 62 3068.5 63 2375.0 64 1685.0 65 1225.0 66 885.0 67 706.0 68 573.0 69 396.5 70 294.5 71 244.5 72 204.5 73 184.5 74 156.0 75 118.0 76 80.0 77 52.0 78 29.5 79 18.0 80 15.5 81 8.0 82 4.0 83 5.0 84 3.5 85 2.5 86 2.0 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2382299240699605 2 0.0 3 0.0 4 0.0 5 0.024834779729300902 6 0.0025294683057621287 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0037942024586431933 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2995166416019352E-4 26-27 9.198066566407741E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00781835658144658 36-37 0.015406761498732968 38-39 0.011957486536330064 40-41 0.009428018230567934 42-43 0.008278259909766968 44-45 0.0036792266265630964 46-47 4.5990332832038705E-4 48-49 0.001494685817041258 50-51 1.1497583208009676E-4 52-53 0.0 54-55 0.0 56-57 0.0012647341528810644 58-59 2.2995166416019352E-4 60-61 0.002299516641601935 62-63 8.048308245606773E-4 64-65 0.001379709984961161 66-67 0.005863767436084935 68-69 2.2995166416019352E-4 70-71 1.1497583208009676E-4 72-73 0.0 74-75 3.4492749624029026E-4 76-77 0.059212553521249836 78-79 0.13072752107507002 80-81 4.5990332832038705E-4 82-83 0.001034782488720871 84-85 0.0 86-87 0.10152365972672545 88-89 0.339868559628766 90-91 0.3771207292227174 92-93 1.1497583208009676E-4 94-95 3.4492749624029026E-4 96-97 1.1497583208009676E-4 98-99 0.0 100-101 0.001034782488720871 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 434874.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.34424283980542 #Duplication Level Percentage of deduplicated Percentage of total 1 87.09263414032738 55.16816966543225 2 8.117426324292273 10.283844486403979 3 1.9047213862733714 3.6195940210281385 4 0.8009973377569766 2.029542795076623 5 0.4744805913594546 1.5027806900923888 6 0.3094094088200763 1.175958283753173 7 0.21077210351173148 0.9345839516092606 8 0.15970261972464472 0.8092993220792796 9 0.1378546607737301 0.7859069197785142 >10 0.7146270201784323 8.383776722934774 >50 0.046497296218629615 2.0398753002460626 >100 0.01997930696448297 2.656094820834117 >500 0.0047223816461505206 2.213373999965639 >1k 0.00581216202603141 7.2022669922990445 >5k 3.632601266269631E-4 1.1949320284667393 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5193 1.194138991983885 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4430 1.0186858722296572 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3843 0.8837042453676237 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2982 0.685715862525697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2446 0.5624617705358333 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2333 0.5364772324857315 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1798 0.41345309216002796 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1687 0.3879284574382465 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1480 0.3403284629570864 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1470 0.33802894631548447 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1387 0.3189429581901884 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1343 0.3088250849671399 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1323 0.30422605168393607 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1313 0.30192653504233413 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1272 0.29249851681176614 RNA PCR Primer, Index 17 (95% over 21bp) TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1182 0.27180286703734874 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1011 0.23248113246595567 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 945 0.21730432263138288 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 928 0.2133951443406596 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 913 0.20994586937825668 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 860 0.19775843117776642 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 790 0.1816618146865529 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 787 0.1809719596940723 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 725 0.1667149565161403 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 694 0.1595864549271743 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 668 0.15360771165900927 RNA PCR Primer, Index 17 (95% over 23bp) TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 636 0.14624925840588307 RNA PCR Primer, Index 17 (96% over 28bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 584 0.13429177186955302 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 573 0.13176230356379087 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 516 0.11865505870665986 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 488 0.11221641211017444 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.10577776551368903 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 453 0.10416810386456768 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 440 0.10117873223048515 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.449274962402903E-4 0.0 22-23 0.0 0.0 0.0 0.0032193232982427092 0.0 24-25 0.0 0.0 0.0 0.00816328407768687 0.0 26-27 0.0 0.0 0.0 0.015866664827053352 0.0 28-29 0.0 0.0 0.0 0.05024443861900228 0.0 30-31 0.0 0.0 0.0 0.12635843945602634 0.0 32-33 0.0 0.0 0.0 0.29491300928544817 0.0 34-35 0.0 0.0 0.0 0.5363622566536514 0.0 36-37 0.0 0.0 0.0 0.8573747798212816 0.0 38-39 0.0 0.0 0.0 1.3191177214549503 0.0 40-41 0.0 0.0 0.0 1.8378886758003468 0.0 42-43 0.0 0.0 0.0 2.326420986308678 0.0 44-45 0.0 0.0 0.0 2.888997732676591 0.0 46-47 0.0 0.0 0.0 3.5268836490569684 0.0 48-49 0.0 0.0 0.0 4.125447830865952 0.0 50-51 0.0 0.0 0.0 4.7804651462262635 0.0 52-53 0.0 0.0 0.0 5.38213367550141 0.0 54-55 0.0 0.0 0.0 6.033816691731397 0.0 56-57 0.0 0.0 0.0 6.80886877578333 0.0 58-59 0.0 0.0 0.0 7.58104646403326 0.0 60-61 0.0 0.0 0.0 8.42611882982197 0.0 62-63 0.0 0.0 0.0 9.222786370304963 0.0 64-65 0.0 0.0 0.0 10.03980463306613 0.0 66-67 0.0 0.0 0.0 10.938685688268325 0.0 68-69 0.0 0.0 0.0 11.955187019688461 0.0 70-71 0.0 0.0 0.0 13.02319752388048 0.0 72-73 0.0 0.0 0.0 14.041768420278057 0.0 74-75 0.0 0.0 0.0 15.077585691487648 0.0 76-77 0.0 0.0 0.0 16.108344026085717 0.0 78-79 0.0 0.0 0.0 17.333181565234987 0.0 80-81 0.0 0.0 0.0 18.579749536647398 0.0 82-83 0.0 0.0 0.0 19.775958093608722 0.0 84-85 0.0 0.0 0.0 20.949861339146512 0.0 86-87 0.0 0.0 0.0 22.1237645846843 0.0 88-89 0.0 0.0 0.0 23.343888114718286 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATTC 75 3.100619E-4 31.622469 3 GACTGCG 60 0.0039775986 31.618834 7 TATCGGC 30 0.0039797677 31.618834 94-95 CTATTAG 105 6.821484E-5 27.186258 1 TCTATAG 105 6.9620044E-5 27.104975 3 CCTCTAT 100 0.0016366991 23.787975 1 CATGGGT 320 0.0 23.716854 4 GTACATG 3175 0.0 23.07621 1 TACATGG 3145 0.0 23.075855 2 CATGGGG 1095 0.0 22.525593 4 ACATGGG 3170 0.0 22.145704 3 ATGGGTA 215 4.471258E-8 22.062187 5 GTGTAAG 130 2.9058548E-4 21.958134 1 GTCCAAG 175 3.9840543E-6 21.749008 1 AGTACTT 4170 0.0 21.560564 12-13 CATAAGC 135 3.8266278E-4 21.081648 4 GTACATA 230 8.83756E-8 20.685196 1 GAGTACT 4200 0.0 20.672298 12-13 ACATAAG 190 8.331843E-6 19.972088 3 ATGGGGA 585 0.0 19.459982 5 >>END_MODULE