FastQCFastQC Report
Thu 26 May 2016
SRR938823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100914
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.9334681015518164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.8145549675961711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.543036645064114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.31809263333135146No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.25269040965574646No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2330.23088966843054481No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.19917949937570603No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1810.17936064371643182No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.16746933032086728No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.1268406762193551No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1020.10107616386229859No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.032.1175081
GTATCAA12700.027.6033521
CCCTTAT350.00830169627.19204568-69
TATCAAC14700.023.5021762
ATCAACG14850.023.264783
TCAACGC15200.022.7290784
CAACGCA15400.022.1265835
GAGTACT8450.021.8212512-13
AACGCAG15500.021.6570036
GTACATG3750.021.4759041
CATGGGG1553.971747E-521.3731674
GAGTAAA1551.0913936E-1121.35197612-13
TACATGG3550.021.3301662
ACGCAGA17000.019.7460927
GTCTCAT600.004879794519.69974110-11
GTACTTT9700.019.25294114-15
CGCAGAG17200.019.2416068
GCAGAGT17200.018.9667269
AGTACTT8550.018.80115512-13
ACATGGG3801.0913936E-1118.6814353
Produced by FastQC (version 0.11.5)