##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938823_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 100914 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.58277345066096 31.0 31.0 34.0 27.0 34.0 2 30.614572804566265 33.0 31.0 34.0 27.0 34.0 3 30.576104405731613 33.0 31.0 34.0 26.0 34.0 4 34.05652337634025 37.0 35.0 37.0 30.0 37.0 5 34.05483877360921 37.0 35.0 37.0 30.0 37.0 6 34.14299304358166 37.0 35.0 37.0 30.0 37.0 7 34.063975266068134 37.0 35.0 37.0 30.0 37.0 8 34.03964762074638 37.0 35.0 37.0 30.0 37.0 9 35.47973522008839 39.0 37.0 39.0 30.0 39.0 10-11 35.394439820044795 39.0 35.0 39.0 30.0 39.0 12-13 35.262961531601164 39.0 35.0 39.0 30.0 39.0 14-15 36.26788156251858 40.0 36.0 41.0 28.0 41.0 16-17 36.1836960183919 40.0 36.0 41.0 27.5 41.0 18-19 36.17016469469053 40.0 36.0 41.0 27.0 41.0 20-21 36.09421388509027 40.0 36.0 41.0 27.5 41.0 22-23 35.89435063519433 40.0 36.0 41.0 27.0 41.0 24-25 35.79232316626038 39.5 35.5 41.0 27.0 41.0 26-27 35.5590155974394 39.0 35.0 41.0 26.5 41.0 28-29 35.33390808014745 39.0 35.0 41.0 25.5 41.0 30-31 34.923147432467246 39.0 35.0 41.0 24.5 41.0 32-33 34.48421428146739 38.0 35.0 41.0 22.5 41.0 34-35 33.97728759141447 38.0 34.0 40.0 17.5 41.0 36-37 33.54551400202152 38.0 34.0 40.0 11.0 41.0 38-39 33.232133301623165 38.0 33.0 40.0 7.0 41.0 40-41 32.87008244643954 38.0 33.0 40.0 2.0 41.0 42-43 32.349550111976534 38.0 33.0 40.0 2.0 41.0 44-45 31.901435876092513 38.0 32.0 40.0 2.0 41.0 46-47 31.382375091662208 38.0 31.0 40.0 2.0 41.0 48-49 30.911627722615297 38.0 30.0 40.0 2.0 41.0 50-51 29.72726777255881 36.5 28.0 39.0 2.0 40.5 52-53 29.724369264918643 36.5 28.0 39.5 2.0 40.5 54-55 30.124620964385517 37.0 28.0 40.0 2.0 41.0 56-57 29.98361971579761 37.0 28.0 40.0 2.0 41.0 58-59 29.76587490338308 36.5 27.5 40.0 2.0 41.0 60-61 29.461531601165348 36.0 26.5 40.0 2.0 41.0 62-63 29.06162673167251 35.5 26.0 39.5 2.0 41.0 64-65 28.675401827298494 35.0 25.5 39.0 2.0 41.0 66-67 28.274342509463505 35.0 23.5 39.0 2.0 41.0 68-69 27.845219692014986 35.0 22.0 38.5 2.0 40.0 70-71 27.4250698614662 35.0 20.5 37.5 2.0 40.0 72-73 27.009577461997345 34.5 19.0 37.0 2.0 39.5 74-75 26.58414095170145 34.0 16.5 36.5 2.0 39.0 76-77 26.14824504033137 34.0 10.0 36.0 2.0 39.0 78-79 25.733500802663656 34.0 4.5 35.5 2.0 37.5 80-81 25.26927879184256 34.0 2.0 35.0 2.0 37.0 82-83 24.83549854331411 33.0 2.0 35.0 2.0 36.5 84-85 24.449164635233963 33.0 2.0 35.0 2.0 36.0 86-87 24.03852785540163 33.0 2.0 35.0 2.0 36.0 88-89 23.672894742057593 32.5 2.0 35.0 2.0 35.0 90-91 23.220237033513683 32.0 2.0 35.0 2.0 35.0 92-93 22.786887845095826 32.0 2.0 35.0 2.0 35.0 94-95 22.2888845948035 31.0 2.0 35.0 2.0 35.0 96-97 21.716709277206334 31.0 2.0 35.0 2.0 35.0 98-99 21.06507025784331 31.0 2.0 34.5 2.0 35.0 100-101 19.326758427968368 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7026711879860592 1101 2 0.26741233090232086 1101 3 0.06782806506206285 1101 4 0.8036161713430303 1101 5 0.7885960105908083 1101 6 -0.2563826608966764 1101 7 -0.7007709828342357 1101 8 0.07307286934295831 1101 9 0.5908029474494683 1101 10-11 0.3022800458013464 1101 12-13 -0.023836756602996445 1101 14-15 0.08259950729038934 1101 16-17 0.37822266788526804 1101 18-19 0.7024210307951435 1101 20-21 0.5279830249861277 1101 22-23 0.07452919240640199 1101 24-25 0.4750486995094647 1101 26-27 0.6709738893493835 1101 28-29 0.2611202282856837 1101 30-31 0.8505361995621286 1101 32-33 0.13023949761620912 1101 34-35 0.4051380030779086 1101 36-37 0.7295258181428395 1101 38-39 0.7627543310149889 1101 40-41 1.4770601919302493 1101 42-43 1.4457342144171008 1101 44-45 1.983630254686176 1101 46-47 2.117690631384203 1101 48-49 2.4962638744313885 1101 50-51 2.1338507839872953 1101 52-53 2.404164960409034 1101 54-55 1.7664036600825028 1101 56-57 2.310704210740159 1101 58-59 2.1730324719707887 1101 60-61 2.1705118723263155 1101 62-63 1.8633915173950868 1101 64-65 1.6223042232254024 1101 66-67 1.9530982929492033 1101 68-69 2.3434100347798825 1101 70-71 1.4062834297776305 1101 72-73 1.5100686138873378 1101 74-75 1.5333087651131017 1101 76-77 1.3053267167908977 1101 78-79 0.3681161245831994 1101 80-81 0.15803135491009357 1101 82-83 0.4921420326455568 1101 84-85 0.42931905048706653 1101 86-87 0.2264942146668112 1101 88-89 0.5385897090968719 1101 90-91 -0.35325070740019626 1101 92-93 -0.3049132384258506 1101 94-95 0.3388316913837741 1101 96-97 -0.15083473107458545 1101 98-99 -0.3259103485429655 1101 100-101 -0.2502278378500584 1102 1 0.0013695971528164819 1102 2 0.2167949096096322 1102 3 0.6195023336125125 1102 4 0.0837363181891817 1102 5 -0.22697858057617282 1102 6 -0.5922375947046632 1102 7 -0.2543364517406772 1102 8 -0.33954254950229057 1102 9 -0.1252945049924108 1102 10-11 -0.353577112948237 1102 12-13 -0.45302799033311203 1102 14-15 -0.0012251673119081374 1102 16-17 0.4402985383936624 1102 18-19 0.3364181383710658 1102 20-21 -0.37862505155186454 1102 22-23 -0.7467523739524822 1102 24-25 -0.05259619981193708 1102 26-27 -0.03516694945360399 1102 28-29 0.029114443437535442 1102 30-31 -0.2097597176700532 1102 32-33 -0.15166060250616198 1102 34-35 -0.017712147105648057 1102 36-37 -0.48890782297408464 1102 38-39 -0.221615454278151 1102 40-41 0.2530085732852356 1102 42-43 0.7234069255084279 1102 44-45 1.6054458070279196 1102 46-47 1.7954634648473196 1102 48-49 1.8325637965584356 1102 50-51 1.346110212177308 1102 52-53 0.32762696953129833 1102 54-55 0.10737595955963997 1102 56-57 -0.31088884744206524 1102 58-59 0.030079974473853355 1102 60-61 0.7352576727490536 1102 62-63 0.3393621481660247 1102 64-65 0.660851336363681 1102 66-67 0.9290133001802623 1102 68-69 1.3999791748399595 1102 70-71 0.4774815608266927 1102 72-73 0.42301777397188545 1102 74-75 -0.4383788530869168 1102 76-77 -0.20401803790929662 1102 78-79 -0.1473472195040202 1102 80-81 0.02865100114438235 1102 82-83 0.06973687078105328 1102 84-85 -0.4680555184305 1102 86-87 -0.43448030975192253 1102 88-89 -0.7586068644930712 1102 90-91 -0.9411359004138795 1102 92-93 -1.3618717432650982 1102 94-95 -1.7710804234232107 1102 96-97 -1.4973137610000506 1102 98-99 -1.021427091228471 1102 100-101 -0.35774814044211567 1103 1 -0.5437005348603847 1103 2 0.06013236835550728 1103 3 -0.25766350797164606 1103 4 -0.5053725927019102 1103 5 -0.321656798397953 1103 6 0.041838312886099516 1103 7 -0.23494701279678765 1103 8 -0.10810690593793737 1103 9 -0.31135061060297176 1103 10-11 -0.3011843736743103 1103 12-13 -0.8028629738314592 1103 14-15 -0.24792483727889447 1103 16-17 0.09340291133095491 1103 18-19 0.08023001955918829 1103 20-21 0.33486504745803813 1103 22-23 -0.29104899111419513 1103 24-25 0.23195413022107658 1103 26-27 -0.46508650390904904 1103 28-29 0.24590487248044468 1103 30-31 -0.0059127704753265675 1103 32-33 -0.09137801174708216 1103 34-35 -0.08093409430036758 1103 36-37 -0.020415661257906947 1103 38-39 -0.0796496952022423 1103 40-41 -0.3305825983319295 1103 42-43 -0.5296871338975109 1103 44-45 0.04016115356257899 1103 46-47 -0.8495963536345243 1103 48-49 -0.9075867809993241 1103 50-51 -0.19421569541345107 1103 52-53 -0.1254464628119365 1103 54-55 -0.37416281931824713 1103 56-57 0.38104597103978577 1103 58-59 0.13651561803820655 1103 60-61 -0.21162764078230012 1103 62-63 -0.12010567361615188 1103 64-65 -0.7980492411940716 1103 66-67 -0.5673253961893749 1103 68-69 -0.46692470304783384 1103 70-71 0.25553436610721647 1103 72-73 -0.425734288734386 1103 74-75 -0.9627703547370814 1103 76-77 -1.356658301935699 1103 78-79 -0.6318253548175541 1103 80-81 -0.3111282892846603 1103 82-83 -0.8026847463806632 1103 84-85 -0.8058752379024483 1103 86-87 -0.31066636585753216 1103 88-89 -0.03052311861848267 1103 90-91 -0.9106594152653678 1103 92-93 -0.7374389874895222 1103 94-95 -1.0498036082416924 1103 96-97 -0.2701582329472423 1103 98-99 -0.08088459947929394 1103 100-101 0.04793461533346388 1104 1 1.4667161318062831 1104 2 1.4663823683555037 1104 3 1.1277531586950218 1104 4 1.9633774072980898 1104 5 1.209593201602047 1104 6 0.8439216462194281 1104 7 1.160886320536548 1104 8 1.058559760728727 1104 9 1.0115660560636996 1104 10-11 1.5373572929923611 1104 12-13 1.6971370261685337 1104 14-15 1.2052001627210984 1104 16-17 1.9788195779976263 1104 18-19 1.9239800195591883 1104 20-21 2.147365047458038 1104 22-23 1.5995760088857978 1104 24-25 1.9923707968877338 1104 26-27 1.488038496090951 1104 28-29 1.4177798724804447 1104 30-31 0.9576288961913377 1104 32-33 0.6690386549195821 1104 34-35 0.023232572366296722 1104 36-37 1.0316676720754216 1104 38-39 0.9828503047977506 1104 40-41 1.164209068334742 1104 42-43 0.8088545327691605 1104 44-45 0.23286948689591114 1104 46-47 -0.1100130203011922 1104 48-49 -0.10029511433265625 1104 50-51 0.3526593045865454 1104 52-53 0.33288687052139565 1104 54-55 -0.3116628193182507 1104 56-57 -0.040829028960217784 1104 58-59 0.40214061803820655 1104 60-61 0.9237890258843713 1104 62-63 0.4788526597171803 1104 64-65 0.691534092139257 1104 66-67 0.4743412704772929 1104 68-69 0.18932529695216616 1104 70-71 0.12532603277388787 1104 72-73 -0.19135928873438246 1104 74-75 -0.05652035473708139 1104 76-77 -0.15353330193569903 1104 78-79 0.4358829785157816 1104 80-81 0.49095504404867185 1104 82-83 0.3587735869526689 1104 84-85 0.6003747620975552 1104 86-87 0.4601669674758 1104 88-89 0.7298935480481852 1104 90-91 0.3914239180679644 1104 92-93 0.8042276791771492 1104 94-95 0.7158213917583041 1104 96-97 0.8756751003860899 1104 98-99 0.8878654005207025 1104 100-101 1.7979346153334603 1105 1 0.4875494651396153 1105 2 0.7892990350221716 1105 3 0.3465031586950218 1105 4 0.5154607406314256 1105 5 0.7304265349353827 1105 6 0.9168383128860995 1105 7 0.26505298720321235 1105 8 1.1418930940620626 1105 9 0.41781605606369965 1105 10-11 1.1727739596590325 1105 12-13 1.613803692835205 1105 14-15 0.8874918293877627 1105 16-17 0.5361112446642977 1105 18-19 1.397938352892524 1105 20-21 1.2046567141247095 1105 22-23 1.6047843422191406 1105 24-25 1.3204957968877338 1105 26-27 1.2901218294242796 1105 28-29 0.9281965391471019 1105 30-31 1.1190872295246663 1105 32-33 1.3461219882529107 1105 34-35 1.3044825723662967 1105 36-37 1.588959338742093 1105 38-39 1.446391971464422 1105 40-41 1.5756674016680776 1105 42-43 0.7984378661024891 1105 44-45 1.8422444868959147 1105 46-47 1.7337369796988078 1105 48-49 1.660121552334008 1105 50-51 1.6807843045865454 1105 52-53 1.9578868705213957 1105 54-55 2.2664621806817493 1105 56-57 1.90708763770645 1105 58-59 2.1573489513715423 1105 60-61 2.0696223592177 1105 62-63 2.2757276597171803 1105 64-65 2.483200758805925 1105 66-67 2.146216270477293 1105 68-69 1.6580752969521662 1105 70-71 2.151367699440552 1105 72-73 2.444057377932282 1105 74-75 2.2820213119295865 1105 76-77 2.4818833647309724 1105 78-79 2.743174645182446 1105 80-81 2.0065800440486683 1105 82-83 2.1973152536193403 1105 84-85 1.923291428764223 1105 86-87 1.5955836341424643 1105 88-89 1.3548935480481852 1105 90-91 2.235173918067968 1105 92-93 2.2833943458438135 1105 94-95 1.5231130584249755 1105 96-97 1.3027584337194256 1105 98-99 1.044115400520706 1105 100-101 0.7979346153334639 1106 1 0.1636858287759786 1106 2 0.010574287547424177 1106 3 0.5418945728364335 1106 4 -0.3791099664392874 1106 5 0.01104522180406775 1106 6 -0.27760613155835046 1106 7 0.06429541144564155 1106 8 0.2088122859812529 1106 9 0.13183120757884836 1106 10-11 -0.29487124236117523 1106 12-13 0.1769350059665129 1106 14-15 0.31015597080190815 1106 16-17 0.1532198305228789 1106 18-19 0.2633108276399909 1106 20-21 -0.053392528299539777 1106 22-23 0.1835406553504484 1106 24-25 0.7082798877968273 1106 26-27 -0.1019236251211737 1106 28-29 0.20613214520771095 1106 30-31 0.31779303760548316 1106 32-33 0.5808126448185718 1106 34-35 1.1517047945885253 1106 36-37 0.9049315609643074 1106 38-39 0.9334500522725051 1106 40-41 1.1670499774256484 1106 42-43 1.358570441860067 1106 44-45 2.0871939818454095 1106 46-47 2.310915010001839 1106 48-49 1.8848690270814892 1106 50-51 1.8850141025663447 1106 52-53 1.6892631331476586 1106 54-55 2.320597281691853 1106 56-57 1.5322454659892806 1106 58-59 1.5733843049068916 1106 60-61 1.7407082178035616 1106 62-63 1.3379120031515228 1106 64-65 1.3747727285028972 1106 66-67 1.3880091997702202 1106 68-69 1.7324124181642908 1106 70-71 1.3893727499456041 1106 72-73 1.5968351557100604 1106 74-75 1.5885238371821089 1106 76-77 1.4893012940238997 1106 78-79 1.9980673219501242 1106 80-81 1.601908326876952 1106 82-83 1.6969995970536829 1106 84-85 1.1302043075520984 1106 86-87 1.5408172200010526 1106 88-89 1.3945084470380813 1106 90-91 1.51310952412857 1106 92-93 0.968211265035734 1106 94-95 1.7672734119603248 1106 96-97 1.9140273731133632 1106 98-99 1.2696520166823184 1106 100-101 1.5935469890708376 1107 1 0.7071007471908999 1107 2 0.6747157016888394 1107 3 0.46108649202835394 1107 4 1.15808894575963 1107 5 0.13507397083281347 1107 6 0.6796588257066105 1107 7 0.031078628228854654 1107 8 0.6034315556005225 1107 9 0.33929041503805735 1107 10-11 0.5529822929923611 1107 12-13 0.5128421543736721 1107 14-15 0.18637003451596712 1107 16-17 0.8766561164591664 1107 18-19 0.9119607887899619 1107 20-21 0.6185188936118848 1107 22-23 1.1604734447832428 1107 24-25 1.269614386631325 1107 26-27 1.432750034552491 1107 28-29 1.2859689750445469 1107 30-31 0.42157120388364433 1107 32-33 0.6966828856888156 1107 34-35 1.2740338544175813 1107 36-37 1.4102734413061881 1107 38-39 1.2420650483875022 1107 40-41 1.7291129144885886 1107 42-43 1.5328128661024891 1107 44-45 0.8791034612548856 1107 46-47 1.2277273643141946 1107 48-49 1.1761471933596503 1107 50-51 0.256906099458341 1107 52-53 0.287213793598319 1107 54-55 -0.08650256290799163 1107 56-57 0.42511648386029677 1107 58-59 0.42016946419205325 1107 60-61 0.10968646178180563 1107 62-63 -0.026355673616151876 1107 64-65 -0.15421911298894742 1107 66-67 0.2616008858619061 1107 68-69 -0.10835098509911134 1107 70-71 0.4061753917482456 1107 72-73 0.25655737793228184 1107 74-75 0.3741687478270208 1107 76-77 0.19302118524378997 1107 78-79 -0.05690548302267828 1107 80-81 0.1476056850743106 1107 82-83 0.13080884336292797 1107 84-85 0.1584677108155006 1107 86-87 0.6440611982450299 1107 88-89 0.6381467531763896 1107 90-91 0.8092925078115556 1107 92-93 0.3230578073822734 1107 94-95 0.022712417399333162 1107 96-97 0.39130010038608987 1107 98-99 1.0220801441104506 1107 100-101 0.27429679482064273 1108 1 0.050874547647868695 1108 2 0.1870919393126016 1108 3 0.27296768014716477 1108 4 -0.11428348379102005 1108 5 0.09975409269115687 1108 6 0.7047921082656359 1108 7 0.26051503340783455 1108 8 0.8287742821808735 1108 9 1.2905470791660107 1108 10-11 0.6563238771507756 1108 12-13 0.8093482472906501 1108 14-15 0.647289684173245 1108 16-17 0.7719817067104984 1108 18-19 1.3512696235195847 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0.06527696533104788 2316 84-85 0.287452464800257 2316 86-87 0.603607282791117 2316 88-89 0.8063293588589922 2316 90-91 1.0511930622121106 2316 92-93 0.9319022287266954 2316 94-95 1.0759002205871369 2316 96-97 0.880038839124829 2316 98-99 0.7075444545747587 2316 100-101 -0.08649669097284374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2962.0 3 540.0 4 424.0 5 375.0 6 390.0 7 384.0 8 486.0 9 518.0 10 620.0 11 918.0 12 1137.0 13 1492.0 14 1547.0 15 1699.0 16 2025.0 17 2239.0 18 1686.0 19 1014.0 20 962.0 21 924.0 22 961.0 23 963.0 24 1107.0 25 1221.0 26 1345.0 27 1523.0 28 1722.0 29 2065.0 30 2411.0 31 2742.0 32 3335.0 33 4222.0 34 5457.0 35 7656.0 36 10282.0 37 15164.0 38 14165.0 39 2231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.291172530144394 19.988091103061578 15.113382622934552 20.607353743859473 2 16.286496350364963 22.746747064423992 38.42728498889241 22.539471596318627 3 20.166800543440534 26.3043068653993 31.476908735707415 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48-49 19.975012568379448 26.952578633257176 31.396558503939755 21.675850294423622 50-51 19.559223165669575 26.92292377985049 31.57507042674126 21.94278262773868 52-53 20.024985814826245 26.966264172730618 31.207382263057827 21.80136774938531 54-55 19.799422655783395 26.417479593868205 31.502588094764082 22.280509655584314 56-57 19.69088529615199 26.892780191181377 31.177193584824913 22.23914092784172 58-59 20.037413121458318 26.482221304583604 31.207120433434994 22.273245140523088 60-61 19.65889760959742 26.65104897191799 31.530335133844638 22.159718284639947 62-63 19.52086153356159 26.70793853013775 31.356994983677044 22.414204952623617 64-65 19.521811203536387 27.169546452412597 30.83934429493785 22.469298049113164 66-67 19.62127396905677 26.970291312399198 30.16865454690269 23.239780171641343 68-69 19.334972916998566 26.95704556316712 30.234387446232276 23.47359407360204 70-71 19.80475517234513 26.46856767358968 29.72779226985802 23.998884884207172 72-73 19.775296559757475 26.0808315122432 29.78092161264791 24.362950315351416 74-75 19.624395852724945 26.521754279428382 29.311160112093898 24.542689755752775 76-77 19.90623460261687 26.039805501500545 29.045872381511696 25.008087514370892 78-79 19.795901105079587 26.073608948109523 29.263462715381056 24.867027231429837 80-81 20.0719521115407 25.935601367388678 28.95500256260978 25.03744395846084 82-83 19.896588104148417 25.734035353133212 29.2230671231786 25.14630941953977 84-85 19.687966557181248 25.56036627849109 29.24405295112969 25.50761421319797 86-87 19.87836075691064 25.53130070375569 29.358158887528496 25.232179651805176 88-89 20.027873177044448 24.878303718082723 29.61176646259519 25.482056642277634 90-91 20.190637365919216 24.526517508275056 30.147084442895896 25.135760682909837 92-93 20.222895854176933 24.421978984674045 30.057590554551744 25.297534606597278 94-95 20.418626645760224 24.185768685134025 30.108265511834542 25.28733915727121 96-97 20.417245344443945 23.6205068470648 30.561653450412912 25.400594358078344 98-99 20.58955290744557 23.927565232107835 30.252167766727396 25.230714093719204 100-101 21.150180415577953 23.06134129650367 30.882169963916883 24.906308324001493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 282.0 1 192.0 2 102.5 3 114.0 4 100.5 5 88.0 6 140.5 7 223.0 8 290.0 9 310.0 10 266.0 11 233.0 12 246.0 13 254.5 14 259.5 15 288.5 16 326.5 17 360.0 18 370.5 19 393.0 20 430.5 21 461.0 22 525.5 23 606.5 24 720.5 25 844.5 26 1009.5 27 1214.5 28 1370.0 29 1568.0 30 1775.0 31 1847.0 32 2001.0 33 2162.0 34 2260.5 35 2417.5 36 2562.0 37 2649.0 38 2680.5 39 2683.0 40 2680.0 41 2675.0 42 2676.5 43 2819.5 44 3018.5 45 3130.5 46 3274.5 47 3482.0 48 3794.5 49 3864.0 50 3575.0 51 3300.0 52 3077.0 53 2890.0 54 2689.0 55 2539.0 56 2274.5 57 1940.5 58 1783.0 59 1560.5 60 1307.5 61 1153.5 62 970.0 63 764.5 64 582.0 65 472.5 66 410.5 67 321.5 68 257.0 69 225.5 70 185.5 71 152.0 72 125.5 73 106.0 74 85.5 75 64.5 76 45.5 77 36.0 78 23.5 79 19.0 80 17.5 81 12.0 82 11.5 83 9.0 84 7.5 85 7.5 86 5.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14765047466159303 2 0.08125730820302435 3 0.07432070872227838 4 0.08819390768377033 5 0.10603087777711714 6 0.038646768535584755 7 0.0426105396674396 8 0.04756525358225816 9 0.044592425233367025 10-11 0.04409695384188517 12-13 0.049547139148185586 14-15 0.0470697821907763 16-17 0.04756525358225816 18-19 0.04806072497374002 20-21 0.04905166775670373 22-23 0.06342033810967755 24-25 0.07828447985413323 26-27 0.08125730820302435 28-29 0.07085240898190538 30-31 0.05896109558634085 32-33 0.04855619636522187 34-35 0.27944586479576666 36-37 0.45385179459738 38-39 0.4360148245040331 40-41 0.41570049745327703 42-43 0.44394236676774285 44-45 0.44790613789959766 46-47 0.4474106665081158 48-49 0.4593019799036804 50-51 0.45087896624848883 52-53 0.4528608518144162 54-55 0.44988802346552514 56-57 0.429573696414769 58-59 0.4107457835384585 60-61 0.38349485700695646 62-63 0.4379967100699606 64-65 0.46822046495035374 66-67 0.46772499355887187 68-69 0.47763442138850903 70-71 0.4716887646907268 72-73 0.46623857938442637 74-75 0.45831103712071664 76-77 0.4464197237251521 78-79 0.4196642685851319 80-81 0.4266008680658778 82-83 0.4379967100699606 84-85 0.439978595635888 86-87 0.4488970806825614 88-89 0.4548427373803437 90-91 0.45732009433775295 92-93 0.4593019799036804 94-95 0.4622748082525715 96-97 0.4652476366014627 98-99 0.46078839407812594 100-101 0.4474106665081158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 100914.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.7774144320907 #Duplication Level Percentage of deduplicated Percentage of total 1 92.1768381736778 76.30160334542283 2 5.470826250389063 9.057217036288325 3 1.1336701223453922 2.8152684463999047 4 0.4477218856992362 1.4824504033137127 5 0.23942346828836164 0.9909427829637116 6 0.13647137692436612 0.6778048635471788 7 0.08140397921804296 0.4716887646907268 8 0.05506739770632318 0.3646669441306459 9 0.049081810999114135 0.3656578869136096 >10 0.17956760121627122 2.6656360861723845 >50 0.016759642780185314 0.9404047010325625 >100 0.009576938731534465 1.5755990249123015 >500 0.0035913520243254244 2.2910597142121016 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 0.9334681015518164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 822 0.8145549675961711 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 548 0.543036645064114 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 321 0.31809263333135146 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.25269040965574646 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 233 0.23088966843054481 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 201 0.19917949937570603 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 181 0.17936064371643182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 169 0.16746933032086728 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.1268406762193551 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 102 0.10107616386229859 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.909427829637117E-4 0.0 0.0 0.0 0.0 7 9.909427829637117E-4 0.0 0.0 0.0 0.0 8 9.909427829637117E-4 0.0 0.0 0.0 0.0 9 9.909427829637117E-4 0.0 0.0 0.0 0.0 10-11 9.909427829637117E-4 0.0 0.0 0.0 0.0 12-13 9.909427829637117E-4 0.0 0.0 0.0 0.0 14-15 9.909427829637117E-4 0.0 0.0 0.0 0.0 16-17 9.909427829637117E-4 0.0 0.0 0.0 0.0 18-19 0.0019818855659274235 0.0 0.0 0.0 0.0 20-21 0.002972828348891135 0.0 0.0 0.0 0.0 22-23 0.002972828348891135 0.0 0.0 0.0034682997403729906 0.0 24-25 0.002972828348891135 0.0 0.0 0.016350555918901243 0.0 26-27 0.002972828348891135 0.0 0.0 0.03418752601224805 0.0 28-29 0.002972828348891135 0.0 0.0 0.09017579324969777 0.0 30-31 0.002972828348891135 0.0 0.0 0.1739104584101314 0.0 32-33 0.002972828348891135 0.0 0.0 0.3175971619398696 0.0 34-35 0.002972828348891135 0.0 0.0 0.5514596587193056 0.0 36-37 0.002972828348891135 0.0 0.0 0.8987851041480865 0.0 38-39 0.002972828348891135 0.0 0.0 1.2703886477594784 0.0 40-41 0.002972828348891135 0.0 0.0 1.6325782349327151 0.0 42-43 0.002972828348891135 0.0 0.0 2.016568563331153 0.0 44-45 0.002972828348891135 0.0 0.0 2.4788433715837246 0.0 46-47 0.002972828348891135 0.0 0.0 2.9891789048100366 0.0 48-49 0.002972828348891135 0.0 0.0 3.4346076857522245 9.909427829637117E-4 50-51 0.002972828348891135 0.0 0.0 3.873595338605149 9.909427829637117E-4 52-53 0.002972828348891135 0.0 0.0 4.317042233981409 9.909427829637117E-4 54-55 0.002972828348891135 0.0 0.0 4.879402263313317 9.909427829637117E-4 56-57 0.002972828348891135 0.0 0.0 5.495273202925263 9.909427829637117E-4 58-59 0.002972828348891135 0.0 0.0 6.138890540460194 9.909427829637117E-4 60-61 0.002972828348891135 0.0 0.0 6.773589392948452 9.909427829637117E-4 62-63 0.002972828348891135 0.0 0.0 7.4167112590919 9.909427829637117E-4 64-65 0.002972828348891135 0.0 0.0 8.136135719523555 9.909427829637117E-4 66-67 0.002972828348891135 0.0 0.0 8.909071090235251 9.909427829637117E-4 68-69 0.002972828348891135 0.0 0.0 9.771191311413679 9.909427829637117E-4 70-71 0.002972828348891135 0.0 0.0 10.66056245912361 9.909427829637117E-4 72-73 0.002972828348891135 0.0 0.0 11.550924549616505 9.909427829637117E-4 74-75 0.002972828348891135 0.0 0.0 12.448718710981627 9.909427829637117E-4 76-77 0.002972828348891135 0.0 0.0 13.390609826188635 9.909427829637117E-4 78-79 0.002972828348891135 0.0 0.0 14.479160473274273 9.909427829637117E-4 80-81 0.003963771131854847 0.0 0.0 15.569197534534355 9.909427829637117E-4 82-83 0.003963771131854847 0.0 0.0 16.59829161464217 9.909427829637117E-4 84-85 0.003963771131854847 0.0 0.0 17.678419248072615 9.909427829637117E-4 86-87 0.003963771131854847 0.0 0.0 18.78728422220901 9.909427829637117E-4 88-89 0.003963771131854847 0.0 0.0 19.939255207404322 9.909427829637117E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 885 0.0 32.117508 1 GTATCAA 1270 0.0 27.603352 1 CCCTTAT 35 0.008301696 27.192045 68-69 TATCAAC 1470 0.0 23.502176 2 ATCAACG 1485 0.0 23.26478 3 TCAACGC 1520 0.0 22.729078 4 CAACGCA 1540 0.0 22.126583 5 GAGTACT 845 0.0 21.82125 12-13 AACGCAG 1550 0.0 21.657003 6 GTACATG 375 0.0 21.475904 1 CATGGGG 155 3.971747E-5 21.373167 4 GAGTAAA 155 1.0913936E-11 21.351976 12-13 TACATGG 355 0.0 21.330166 2 ACGCAGA 1700 0.0 19.746092 7 GTCTCAT 60 0.0048797945 19.699741 10-11 GTACTTT 970 0.0 19.252941 14-15 CGCAGAG 1720 0.0 19.241606 8 GCAGAGT 1720 0.0 18.966726 9 AGTACTT 855 0.0 18.801155 12-13 ACATGGG 380 1.0913936E-11 18.681435 3 >>END_MODULE