FastQCFastQC Report
Thu 26 May 2016
SRR938823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100914
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT19711.9531482252214758No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT10361.0266167231504053TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.804645539766534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.7412252016568563No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG6040.5985294409100819TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT5660.5608736151574608RNA PCR Primer, Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.5301543888855857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.31710169054838777No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2330.23088966843054481No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.2189983550349803No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.20215232772459718No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.20017044215866975No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT1980.1962066710268149TruSeq Adapter, Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1750.17341498701864955No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1510.14963236022752047TruSeq Adapter, Index 11 (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1480.14665953187862935No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1300.1288225617852825No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA1270.1258497334363914RNA PCR Primer, Index 11 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.11296747725786313No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1030.1020671066452623No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGAC459.6403545E-442.1713453
TAATAGT500.001618395437.9542124
CGCAGAA702.0546325E-433.887699
GGTATCA11250.031.362211
GTCCAGG804.3675117E-429.7993951
GCAAGAG856.411487E-427.9075093
AGTGGGA700.0084052627.1101538
CAGTACA700.0084052627.1101534
TCCAGGG1109.470212E-525.877872
GTATCAA13750.025.5328332
ATCAACG15850.022.1499354
TCAACGC16100.022.1006665
CAACGCA16100.021.805996
TATCAAC16200.021.6713853
AACGCAG16400.021.40717
TGCTTGA7100.020.71444754-55
CTTGAAA6800.020.58178956-57
GAGTACT7200.020.42674412-13
CCCCGAG600.004783394719.76781878-79
ACGCAGA18000.018.7135358
Produced by FastQC (version 0.11.5)