FastQCFastQC Report
Thu 26 May 2016
SRR938822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99979
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.9261945008451775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.7701617339641325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.5561167845247502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.3190670040708549No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.25205293111553423No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2360.23604957040978605No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.2050430590423989No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.19704137868952482No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1730.17303633763090248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.1400294061752968No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1160.1160243651166745No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.1000210044109263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG200.002078275271.1655352
GCGTATG200.002078275271.1655358
TACATCG250.00503146656.9324345
GGTATCA6800.043.3008271
GCCGTAT300.00393709231.67669392-93
ATGGGGA753.0798675E-431.629135
GTATCAA9600.030.640722
ATCAACG11700.025.5466044
TATCAAC11550.025.4676113
TCAACGC12300.024.3004285
CAACGCA12350.024.2020476
AACGCAG12650.023.6280867
GTACATG4150.022.887341
TACATGG3950.021.6199112
GAGTACT8250.021.57611512-13
CATGGGG1656.306391E-520.1276284
ACGCAGA15200.019.6641628
CGCAGAG15000.019.610069
GTACTTT9700.018.84017614-15
AGTACAT3305.857146E-1018.689947
Produced by FastQC (version 0.11.5)