##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938822_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99979 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.505566168895466 31.0 31.0 34.0 27.0 34.0 2 30.547915062163053 33.0 31.0 34.0 26.0 34.0 3 30.501085227897857 33.0 31.0 34.0 26.0 34.0 4 33.98054591464207 37.0 35.0 37.0 30.0 37.0 5 33.98163614359015 37.0 35.0 37.0 30.0 37.0 6 34.047279928785045 37.0 35.0 37.0 30.0 37.0 7 33.97006371337981 37.0 35.0 37.0 30.0 37.0 8 33.93243581152042 37.0 35.0 37.0 30.0 37.0 9 35.3861110833275 39.0 35.0 39.0 30.0 39.0 10-11 35.28036387641404 39.0 35.0 39.0 30.0 39.0 12-13 35.16991068124307 39.0 35.0 39.0 29.0 39.0 14-15 36.16979565708799 40.0 36.0 41.0 27.0 41.0 16-17 36.042803988837655 40.0 36.0 41.0 27.0 41.0 18-19 36.05638184018644 40.0 36.0 41.0 27.0 41.0 20-21 35.9774852719071 40.0 36.0 41.0 27.0 41.0 22-23 35.84352213964932 40.0 36.0 41.0 27.0 41.0 24-25 35.70557317036578 39.5 35.5 41.0 27.0 41.0 26-27 35.450544614369015 39.0 35.0 41.0 25.5 41.0 28-29 35.18411866491964 39.0 35.0 41.0 24.5 41.0 30-31 34.760644735394436 38.5 35.0 41.0 24.0 41.0 32-33 34.34583762590144 38.0 35.0 40.5 21.5 41.0 34-35 33.848518188819654 38.0 34.0 40.0 16.0 41.0 36-37 33.48834255193591 38.0 33.5 40.0 11.0 41.0 38-39 33.15718800948199 38.0 33.0 40.0 5.0 41.0 40-41 32.80950999709939 38.0 33.0 40.0 2.0 41.0 42-43 32.48208123705978 38.0 33.0 40.0 2.0 41.0 44-45 32.02376999169826 38.0 32.5 40.0 2.0 41.0 46-47 31.424684183678572 38.0 31.0 40.0 2.0 41.0 48-49 30.88612608647816 38.0 30.0 40.0 2.0 41.0 50-51 29.712229568209324 36.5 28.0 39.0 2.0 40.5 52-53 29.710944298302643 36.5 28.0 39.5 2.0 40.5 54-55 30.085743006031265 37.0 28.0 40.0 2.0 41.0 56-57 29.89966392942518 37.0 28.0 40.0 2.0 41.0 58-59 29.675656887946467 36.5 27.0 40.0 2.0 41.0 60-61 29.345897638504084 36.0 26.0 40.0 2.0 41.0 62-63 28.96218205823223 35.5 26.0 39.5 2.0 41.0 64-65 28.549125316316427 35.0 24.5 39.0 2.0 41.0 66-67 28.137933966132888 35.0 22.5 39.0 2.0 40.5 68-69 27.71468508386761 35.0 21.5 38.0 2.0 40.0 70-71 27.29413176767121 35.0 20.0 37.5 2.0 40.0 72-73 26.869037497874555 34.0 18.5 37.0 2.0 39.5 74-75 26.39522799787955 34.0 12.5 36.5 2.0 39.0 76-77 25.94442332889907 34.0 7.5 36.0 2.0 39.0 78-79 25.4917732723872 34.0 2.0 35.0 2.0 37.5 80-81 25.029246141689754 33.0 2.0 35.0 2.0 37.0 82-83 24.59410976305024 33.0 2.0 35.0 2.0 36.5 84-85 24.242896008161715 33.0 2.0 35.0 2.0 36.0 86-87 23.805429140119422 32.5 2.0 35.0 2.0 36.0 88-89 23.316116384440733 32.0 2.0 35.0 2.0 35.0 90-91 22.842791986317128 32.0 2.0 35.0 2.0 35.0 92-93 22.351288770641837 31.0 2.0 35.0 2.0 35.0 94-95 21.839196231208554 31.0 2.0 35.0 2.0 35.0 96-97 21.25585372928315 31.0 2.0 34.0 2.0 35.0 98-99 20.58311745466548 30.0 2.0 34.0 2.0 35.0 100-101 18.673086348133108 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25682687268517057 1101 2 0.20403876298490786 1101 3 0.4071912936486335 1101 4 0.9436475593905698 1101 5 -0.12701041784230682 1101 6 0.1279375961826119 1101 7 0.40791553580434936 1101 8 0.5845538153007794 1101 9 0.5041511809172476 1101 10-11 0.23308432428274273 1101 12-13 0.763261014408414 1101 14-15 0.3865683076553026 1101 16-17 0.6092529055501146 1101 18-19 0.8566229447332248 1101 20-21 0.9523218165273235 1101 22-23 0.5426268389685802 1101 24-25 0.39072273613447805 1101 26-27 0.7740272252428468 1101 28-29 0.46405428807547366 1101 30-31 0.7466244439206164 1101 32-33 0.8062148019379194 1101 34-35 0.2639871310856492 1101 36-37 0.9421212697409871 1101 38-39 0.19298130532101254 1101 40-41 0.20295513345484295 1101 42-43 0.21864942916685948 1101 44-45 0.4262098135808259 1101 46-47 0.3787928412371855 1101 48-49 1.21856359189146 1101 50-51 1.2042724012579242 1101 52-53 1.1038976701059475 1101 54-55 0.3731271435357968 1101 56-57 0.4548710279991788 1101 58-59 0.697796263875631 1101 60-61 1.1681602097468868 1101 62-63 0.7122266917766602 1101 64-65 0.045383620121683066 1101 66-67 0.43234834621106444 1101 68-69 0.4449427765434635 1101 70-71 0.48173429302481807 1101 72-73 -0.34064264054206106 1101 74-75 -0.6871631743678002 1101 76-77 -0.791650466175664 1101 78-79 -0.858169602402981 1101 80-81 -0.40360072011038284 1101 82-83 0.018028669352293747 1101 84-85 -0.4470973651615928 1101 86-87 -0.2158782758221065 1101 88-89 -0.11240503651250577 1101 90-91 -0.36198662014946237 1101 92-93 0.08597961587543068 1101 94-95 0.9826434770757189 1101 96-97 0.6729134796416929 1101 98-99 -0.39766568837957195 1101 100-101 0.800537246885284 1102 1 0.07645406566762958 1102 2 0.023117710353329102 1102 3 -0.6875455484566295 1102 4 0.017441419039691652 1102 5 -0.1273393652107302 1102 6 0.3414244382878735 1102 7 -0.49274235893249596 1102 8 -0.2408847811904522 1102 9 0.040335391443562685 1102 10-11 -0.21888935992778613 1102 12-13 -0.05214468734597233 1102 14-15 0.16112971116407948 1102 16-17 0.14839764239221864 1102 18-19 0.1934650499963837 1102 20-21 -0.23687774487618896 1102 22-23 -0.2944893891015923 1102 24-25 -0.7859220007076289 1102 26-27 -1.0797556694939914 1102 28-29 -0.943237378591185 1102 30-31 -1.7142856437986893 1102 32-33 -1.516701864728745 1102 34-35 -1.417153219791551 1102 36-37 -1.0202142565748034 1102 38-39 -1.1543871157316232 1102 40-41 -0.5104768840890159 1102 42-43 -0.45103258837700366 1102 44-45 0.25362209428258353 1102 46-47 0.14211520965823965 1102 48-49 0.31538376733006146 1102 50-51 -0.6172736513736545 1102 52-53 0.07703363501822835 1102 54-55 -0.08378075120104711 1102 56-57 -0.28208072638679127 1102 58-59 -0.7603177712120903 1102 60-61 -0.4463682990250426 1102 62-63 -0.6065233082233412 1102 64-65 0.178113883279579 1102 66-67 -0.07121525028016151 1102 68-69 0.10486602215749485 1102 70-71 0.43299525793710103 1102 72-73 0.38139683314215134 1102 74-75 0.14161972036903947 1102 76-77 0.5945885689120551 1102 78-79 -0.3251652164380694 1102 80-81 -0.7281621236191533 1102 82-83 -0.8481994008231446 1102 84-85 -0.04331447042475034 1102 86-87 0.33779496979192913 1102 88-89 -0.5740278435300539 1102 90-91 -1.321306795588061 1102 92-93 -0.784031349036848 1102 94-95 -0.757104329941825 1102 96-97 -1.001757572989888 1102 98-99 -1.0751327936427302 1102 100-101 -0.49458336714980433 1103 1 0.3492327866636664 1103 2 0.423796827501036 1103 3 0.5792343044013215 1103 4 0.4386072368099292 1103 5 0.8504761412974773 1103 6 0.534859639193364 1103 7 0.19622198741725327 1103 8 -0.031037582548684384 1103 9 0.5451458045731599 1103 10-11 0.6920897006268305 1103 12-13 0.4311037563439015 1103 14-15 0.5774285227090701 1103 16-17 0.5625459163028026 1103 18-19 0.7544724070988167 1103 20-21 0.8886457412585003 1103 22-23 0.18694807552772374 1103 24-25 0.827886714629102 1103 26-27 1.1414667413718789 1103 28-29 1.2266550945270893 1103 30-31 1.3556634224152333 1103 32-33 1.3220078126906145 1103 34-35 1.725008636461986 1103 36-37 1.5736736890958198 1103 38-39 1.4456694773640208 1103 40-41 1.7209322839924752 1103 42-43 1.6717744291668524 1103 44-45 1.2844087383120169 1103 46-47 1.648114077796322 1103 48-49 -0.011611139291332506 1103 50-51 0.8264162184622279 1103 52-53 0.7097444442994956 1103 54-55 1.0750760682669807 1103 56-57 1.078190920472295 1103 58-59 1.2293822853810035 1103 60-61 0.3548201559834432 1103 62-63 0.6708960466153684 1103 64-65 1.0704172222722192 1103 66-67 0.3569115182540763 1103 68-69 0.4834172389090554 1103 70-71 -0.4848315671902341 1103 72-73 0.7215213379525629 1103 74-75 0.6806123632666079 1103 76-77 -0.05425127262727969 1103 78-79 -0.30003788197287307 1103 80-81 -0.29607383838995105 1103 82-83 0.10539426075014546 1103 84-85 -0.5484078490325608 1103 86-87 0.050754788694025166 1103 88-89 0.1826218452079189 1103 90-91 0.45622574544193384 1103 92-93 0.21551590619801075 1103 94-95 0.07051310073163108 1103 96-97 0.1591365979212611 1103 98-99 -0.17269929053010813 1103 100-101 0.5197912791433446 1104 1 -0.22065968645461354 1104 2 -0.23211715099358798 1104 3 -0.17345386764169035 1104 4 -0.18504867716856666 1104 5 -0.33231955762725107 1104 6 -0.0995489629571722 1104 7 -0.41668123838920224 1104 8 -0.23533865781750052 1104 9 -0.422596130910712 1104 10-11 -0.2272651380828492 1104 12-13 -0.09577796408620998 1104 14-15 0.0828048667950938 1104 16-17 0.5356641958726911 1104 18-19 0.9050100415074169 1104 20-21 0.35638767674237215 1104 22-23 0.46114162391481983 1104 24-25 0.585951230758134 1104 26-27 0.5554452359955349 1104 28-29 -0.009904045257862037 1104 30-31 0.45781396004964137 1104 32-33 0.7521153395723275 1104 34-35 0.2733957332361783 1104 36-37 0.6166844417839954 1104 38-39 0.5693253913425167 1104 40-41 -0.04788492030860425 1104 42-43 -0.3174728826611002 1104 44-45 -0.3876342724406783 1104 46-47 0.21263020682858524 1104 48-49 -0.31806275219456026 1104 50-51 -0.03379883530121219 1104 52-53 0.19899175612745168 1104 54-55 -0.08621425431366703 1104 56-57 0.42227694197767107 1104 58-59 0.6487371240906832 1104 60-61 1.032239510822155 1104 62-63 1.2031541111315 1104 64-65 1.333858082487275 1104 66-67 0.93218033545838 1104 68-69 1.2898688518122796 1104 70-71 0.40764155108933764 1104 72-73 -0.11718833946678942 1104 74-75 0.3741607503633837 1104 76-77 0.6823078671576681 1104 78-79 0.5279191072744389 1104 80-81 0.6662917530078971 1104 82-83 0.3634587768791775 1104 84-85 -0.33873042967772093 1104 86-87 -0.12666456614468657 1104 88-89 0.40305195273480265 1104 90-91 0.418591336839782 1104 92-93 0.5703546158754307 1104 94-95 0.16728729428001898 1104 96-97 0.212900038781477 1104 98-99 0.5369781288247317 1104 100-101 0.831619236132596 1105 1 0.7736868383209092 1105 2 0.876074845459133 1105 3 0.7473974792156426 1105 4 0.43838553189916496 1105 5 -0.0029769126876644236 1105 6 0.17658450339910559 1105 7 0.5683536801342441 1105 8 0.5991586262973385 1105 9 -0.0931426335157397 1105 10-11 1.0726461799528408 1105 12-13 1.0824727841678694 1105 14-15 0.6022040464869249 1105 16-17 0.9626678540037261 1105 18-19 0.8669322230837437 1105 20-21 0.812878088004986 1105 22-23 1.2645447942950412 1105 24-25 0.5276428392272621 1105 26-27 0.563814596376865 1105 28-29 0.4196492193469581 1105 30-31 0.6825673133364205 1105 32-33 0.7616486507351681 1105 34-35 1.3228359283365023 1105 36-37 2.095955032627586 1105 38-39 1.2806101713003955 1105 40-41 0.4068318516679028 1105 42-43 0.5085441886170301 1105 44-45 0.3684348994914828 1105 46-47 0.4868791814433706 1105 48-49 1.6649768221320116 1105 50-51 0.9727968857940077 1105 52-53 0.826084096222786 1105 54-55 0.34638745281414884 1105 56-57 0.4446691379648122 1105 58-59 -0.1473497842343363 1105 60-61 -0.1475077455795777 1105 62-63 0.3182189598178944 1105 64-65 0.0030189294000351197 1105 66-67 -0.03323369846247459 1105 68-69 -0.2529201959651317 1105 70-71 0.3851385026468144 1105 72-73 0.776947565643507 1105 74-75 0.630008217384777 1105 76-77 0.7991463551301763 1105 78-79 0.9445580608272586 1105 80-81 0.9761243658002741 1105 82-83 1.2014479133385478 1105 84-85 1.234764748240469 1105 86-87 1.5614520506383762 1105 88-89 1.0046482280923001 1105 90-91 1.3249764382697826 1105 92-93 1.5617635505833363 1105 94-95 0.9597160715774358 1105 96-97 1.1274882219097364 1105 98-99 0.8225340538884716 1105 100-101 1.5431811472289247 1106 1 -0.12775646064816115 1106 2 0.22153876298490616 1106 3 0.2871912936486325 1106 4 0.26323089272390376 1106 5 -0.395760417842304 1106 6 0.4966875961826105 1106 7 0.5004155358043505 1106 8 0.3712204819674412 1106 9 -0.013348819082750651 1106 10-11 0.40558432428274216 1106 12-13 0.7918026810750831 1106 14-15 0.5582349743219694 1106 16-17 0.8305029055501123 1106 18-19 0.6366229447332259 1106 20-21 0.8912801498606555 1106 22-23 1.128668505635254 1106 24-25 0.9719727361344752 1106 26-27 0.2834022252428454 1106 28-29 -0.46823737859119063 1106 30-31 0.4130827772539405 1106 32-33 0.3032981352712554 1106 34-35 0.9373204644189812 1106 36-37 1.198996269740988 1106 38-39 2.0179813053210154 1106 40-41 1.5789968001215016 1106 42-43 1.6734410958335246 1106 44-45 1.557043146914161 1106 46-47 1.3731678412371906 1106 48-49 1.0106469252247976 1106 50-51 0.816147401257922 1106 52-53 0.6859810034392808 1106 54-55 0.7293771435357961 1106 56-57 1.300287694665844 1106 58-59 0.9736295972089621 1106 60-61 1.5133685430802188 1106 62-63 1.4884766917766612 1106 64-65 1.0910086201216842 1106 66-67 0.6608900128777329 1106 68-69 0.9522344432101271 1106 70-71 0.8669426263581528 1106 72-73 1.0706073594579415 1106 74-75 1.263461825632202 1106 76-77 1.2006412004910025 1106 78-79 1.0314137309303497 1106 80-81 0.9997326132229531 1106 82-83 0.274695336018965 1106 84-85 -0.08647236516159396 1106 86-87 0.07016339084455936 1106 88-89 0.07676163015415938 1106 90-91 -0.14656995348279978 1106 92-93 0.33702128254209995 1106 94-95 0.6486851437423837 1106 96-97 0.17166347964169404 1106 98-99 0.5467093116204289 1106 100-101 0.6869955802186176 1107 1 -0.5363278892195886 1107 2 -0.6594816451783565 1107 3 -0.5213801349227936 1107 4 -0.9647282909495658 1107 5 -0.6875971525361848 1107 6 -1.1227001589194288 1107 7 -0.7132579335834066 1107 8 -0.6110244159917428 1107 9 -0.9333488190827524 1107 10-11 -0.8294156757172573 1107 12-13 -1.2323809923942974 1107 14-15 -1.314111964453538 1107 16-17 -0.9207215842458041 1107 18-19 -0.40868317771575136 1107 20-21 -0.32882189095567327 1107 22-23 0.06356646481892625 1107 24-25 0.01891151164468141 1107 26-27 -0.2339447135326651 1107 28-29 -0.8075230928769059 1107 30-31 -0.5740600798889162 1107 32-33 -0.5728243137083382 1107 34-35 -0.37461831109122556 1107 36-37 -0.34008536291207037 1107 38-39 -1.1896717559034755 1107 40-41 -0.42916646518461476 1107 42-43 -0.7891099245746389 1107 44-45 -0.7289772612491063 1107 46-47 -0.8766280771301638 1107 48-49 -0.7548632788568383 1107 50-51 -0.9733423946604418 1107 52-53 -0.787896547581127 1107 54-55 -0.29715346870910153 1107 56-57 -0.2643041420688519 1107 58-59 -0.16402346401552492 1107 60-61 -0.16591717120549632 1107 62-63 -0.15958453271313644 1107 64-65 -0.007868930898723647 1107 66-67 -0.9484977422243084 1107 68-69 -0.8038880057694655 1107 70-71 -1.061526761396948 1107 72-73 -0.38337223237879314 1107 74-75 -0.9725585825310681 1107 76-77 -1.4996649219579794 1107 78-79 -1.6211372894778115 1107 80-81 -1.2029204480015352 1107 82-83 -0.843365888470835 1107 84-85 -0.9238193039371048 1107 86-87 -0.6923876295636013 1107 88-89 -0.6919118392335974 1107 90-91 0.08975657712944596 1107 92-93 -0.37012157460075557 1107 94-95 -0.047437305237210126 1107 96-97 0.04727572453965223 1107 98-99 0.13007665855920436 1107 100-101 0.4747506822594332 1108 1 0.15628848317206234 1108 2 0.21176348208603102 1108 3 -0.09842668387945608 1108 4 -0.179128657837893 1108 5 0.3087339641801634 1108 6 -0.22072813415447 1108 7 -0.17565187992598652 1108 8 -0.05495929331345195 1108 9 0.13968488878241914 1108 10-11 0.4121573579906084 1108 12-13 0.05264537770429456 1108 14-15 -0.342832441408369 1108 16-17 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-1.8822053119946034 2316 90-91 -2.0778096229042866 2316 92-93 -1.7505820232430267 2316 94-95 -1.4561908893154687 2316 96-97 -1.415154702176487 2316 98-99 -1.7159766387927924 2316 100-101 -1.5927564858970875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3123.0 3 533.0 4 452.0 5 381.0 6 401.0 7 433.0 8 465.0 9 496.0 10 600.0 11 871.0 12 1148.0 13 1437.0 14 1500.0 15 1686.0 16 1912.0 17 2055.0 18 1720.0 19 1074.0 20 999.0 21 964.0 22 1005.0 23 1098.0 24 1127.0 25 1257.0 26 1416.0 27 1628.0 28 1815.0 29 2084.0 30 2450.0 31 2888.0 32 3339.0 33 4181.0 34 5387.0 35 7510.0 36 10481.0 37 14894.0 38 13157.0 39 2011.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.221221452141904 19.959679441530177 15.208473335272465 20.610625771055453 2 16.452753814862685 22.53348963499554 38.50030558978829 22.51345096035348 3 20.17877901150439 26.160460175572215 31.57894736842105 22.081813444502345 4 13.422576701869701 18.411454680266928 36.846956974810126 31.319011643053248 5 12.7217125382263 37.60766029979445 33.95698601293427 15.71364114904497 6 28.23126183818887 36.58939899582093 17.862762193960897 17.316576972029303 7 23.85960339261725 35.67862370420869 22.654542538046638 17.807230365127424 8 24.55507093798566 33.38847947059708 21.291422269012884 20.76502732240437 9 25.006761291356565 17.85281419971352 23.057506035078582 34.08291847385133 10-11 23.36799378617424 27.70163614041242 27.46009871965122 21.470271353762122 12-13 25.45266840066353 26.710567858914796 27.858714336545738 19.978049403875932 14-15 22.539275408784867 27.56241984610452 28.589391631933314 21.3089131131773 16-17 19.43976411120472 30.49003089019938 29.396337304930398 20.673867693665503 18-19 19.001192994415984 28.75818303942897 32.77225836850495 19.468365597650102 20-21 20.205649343816237 27.876409955753324 32.704960338339276 19.21298036209117 22-23 19.870557624917907 27.87322467927669 32.506980964651504 19.749236731153903 24-25 19.797498809553645 28.06897070248866 32.83827472995664 19.295255758001055 26-27 19.52893714813291 27.841781488654956 33.26542695715877 19.363854406053367 28-29 19.21963650592195 28.587911288582994 32.899575104217355 19.292877101277696 30-31 19.485209296726886 27.88666980971201 32.770761653509346 19.85735924005176 32-33 19.41564656187522 28.179444909330513 32.56585375630728 19.839054772486985 34-35 19.8425086198676 27.953967146635616 32.42475495485549 19.7787692786413 36-37 19.968168216415965 27.35374450223927 32.6566987327536 20.02138854859117 38-39 19.586692232785534 27.482406561524332 32.704721366114185 20.22617983957595 40-41 19.9444065347409 27.398346244932366 32.5256893991089 20.13155782121784 42-43 19.678253231271174 27.381101769356253 31.914669343706866 21.025975655665704 44-45 19.929820686331603 27.07074155137447 31.976767534788458 21.022670227505472 46-47 20.228013192837313 27.18838949994729 31.591524053835613 20.992073253379786 48-49 20.279172524603332 26.79704759991966 31.407913235589476 21.515866639887527 50-51 19.731689193260095 26.934508866708175 31.60333781856888 21.730464121462855 52-53 19.78390536822445 27.034924587793462 31.252384873375778 21.92878517060631 54-55 19.73803116716202 26.573419551771227 31.515081532653227 22.17346774841353 56-57 19.878133250348835 26.91106939578586 31.080036539948004 22.130760813917302 58-59 20.129557337401025 26.610936606219955 31.179563860427308 22.07994219595171 60-61 19.634920794165154 26.87681210309299 31.609097384552104 21.879169718189754 62-63 19.518488398943806 26.849090890836617 31.40819051634991 22.224230193869662 64-65 19.501968424858394 27.055798818945085 30.89573374040895 22.54649901578757 66-67 19.507761222511814 27.01688838001115 30.056595909225585 23.41875448825145 68-69 19.486545936661944 26.81371219654282 30.270889203603822 23.42885266319141 70-71 19.774905080455614 26.482050663934594 29.891118744852246 23.85192551075755 72-73 19.81670725889472 26.147588319481756 29.779797624726946 24.25590679689658 74-75 19.638848855835814 26.789561165204557 29.131620309228133 24.439969669731497 76-77 19.998694667717626 26.057946712319826 29.16162523910173 24.781733380860814 78-79 20.032322340057114 26.110327589927877 29.02285149290063 24.834498577114378 80-81 20.159221760976614 25.9553556638674 28.841124591529926 25.044297983626056 82-83 20.03976023374198 25.581593823107124 29.266443768386598 25.1122021747643 84-85 19.830109394689412 25.48861120454648 29.259439622063688 25.421839778700416 86-87 19.72248856670967 25.374123364073114 29.557879306623025 25.34550876259419 88-89 20.11727967386611 25.04342761896155 29.487102248195118 25.35219045897722 90-91 19.97147720938641 24.705858721797338 29.92331989213564 25.39934417668061 92-93 20.250977950296523 24.65740354823969 29.844180756348514 25.24743774511527 94-95 20.25150536608394 24.278202700870324 30.118370237192458 25.351921695853274 96-97 20.266076056168263 23.855441049438518 30.44506719701079 25.43341569738243 98-99 20.833563511633628 23.836762571501747 30.047559021901254 25.282114894963364 100-101 21.16908154351297 23.01513188942776 30.860720346216024 24.95506622084325 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 317.0 1 212.5 2 110.5 3 110.5 4 107.0 5 100.0 6 139.5 7 226.5 8 308.0 9 339.5 10 286.5 11 244.0 12 249.0 13 251.0 14 260.5 15 276.0 16 292.0 17 322.0 18 336.5 19 374.0 20 415.5 21 451.5 22 510.5 23 603.0 24 709.5 25 828.5 26 975.0 27 1139.0 28 1378.0 29 1566.0 30 1715.5 31 1895.0 32 2012.5 33 2108.0 34 2209.0 35 2394.0 36 2611.0 37 2731.0 38 2734.0 39 2670.5 40 2652.0 41 2672.0 42 2648.5 43 2750.5 44 2899.5 45 3031.5 46 3153.5 47 3343.0 48 3795.5 49 3885.5 50 3505.0 51 3156.0 52 2947.5 53 2845.5 54 2714.0 55 2493.0 56 2253.5 57 2004.0 58 1802.0 59 1607.0 60 1371.5 61 1146.0 62 948.0 63 762.0 64 595.5 65 500.0 66 411.0 67 324.5 68 262.0 69 205.5 70 169.5 71 147.0 72 132.0 73 110.5 74 79.5 75 58.5 76 56.5 77 52.0 78 37.0 79 25.0 80 13.0 81 8.0 82 11.0 83 7.5 84 3.0 85 4.0 86 5.0 87 4.5 88 3.0 89 2.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2780583922623751 2 0.17203612758679324 3 0.19104011842486923 4 0.17703717780733955 5 0.24405125076266015 6 0.19604116864541551 7 0.23304894027745826 8 0.24405125076266015 9 0.14603066643995238 10-11 0.2015423238880165 12-13 0.2095440042408906 14-15 0.17103591754268396 16-17 0.27105692195361025 18-19 0.23004831014513047 20-21 0.19754148371157942 22-23 0.24355114574060555 24-25 0.22654757499074807 26-27 0.3320697346442753 28-29 0.30756458856359836 30-31 0.28806049270346773 32-33 0.19354064353514236 34-35 0.3545744606367337 36-37 0.39308254733494036 38-39 0.36807729623220875 40-41 0.327068684423729 42-43 0.36657698116604487 44-45 0.37707918662919215 46-47 0.3795797117394653 48-49 0.39908380759959594 50-51 0.39308254733494036 52-53 0.39308254733494036 54-55 0.3870812870702847 56-57 0.36207603596755317 58-59 0.3320697346442753 60-61 0.30206343332099744 62-63 0.3760789765850829 64-65 0.40708548795247 66-67 0.41358685323918026 68-69 0.41808779843767196 70-71 0.42108842856999973 72-73 0.4115864331509617 74-75 0.4085858030186339 76-77 0.3875813920923394 78-79 0.35757509076906147 80-81 0.36957761129837263 82-83 0.38208023684973846 84-85 0.38558097200412084 86-87 0.3795797117394653 88-89 0.38808149711439405 90-91 0.4095860130627431 92-93 0.4085858030186339 94-95 0.4185879034597265 96-97 0.42108842856999973 98-99 0.4185879034597265 100-101 0.38808149711439405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 99979.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.66435951549825 #Duplication Level Percentage of deduplicated Percentage of total 1 92.14853533703582 76.17399653927325 2 5.472672934286786 9.047900059012392 3 1.1434171839268212 2.8355954750497605 4 0.462206734666715 1.5283209473989539 5 0.23594322842934407 0.9752047930065314 6 0.146405798153593 0.7261524920233249 7 0.09437729137173763 0.5461146840836576 8 0.05081854150785872 0.33607057482071234 9 0.03508899294590245 0.26105482151251763 >10 0.17544496472951226 2.4785204893027535 >50 0.021779374931939453 1.2002520529311156 >100 0.00967972219197309 1.6383440522509725 >500 0.003629895821989909 2.25247301933406 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 926 0.9261945008451775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 770 0.7701617339641325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 556 0.5561167845247502 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 319 0.3190670040708549 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252 0.25205293111553423 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 236 0.23604957040978605 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.2050430590423989 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.19704137868952482 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 173 0.17303633763090248 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.1400294061752968 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 116 0.1160243651166745 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.1000210044109263 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.001000210044109263 0.0 20-21 0.0 0.0 0.0 0.003000630132327789 0.0 22-23 0.0 0.0 0.0 0.012002520529311155 0.0 24-25 0.0 0.0 0.0 0.022004620970403784 0.0 26-27 0.0 0.0 0.0 0.04350913691875294 0.0 28-29 0.0 0.0 0.0 0.08351753868312345 0.0 30-31 0.0 0.0 0.0 0.1555326618589904 0.0 32-33 0.0 0.0 0.0 0.2905610178137409 0.0 34-35 5.001050220546315E-4 0.0 0.0 0.5046059672531231 0.0 36-37 0.001000210044109263 0.0 0.0 0.8101701357285029 0.0 38-39 0.001000210044109263 0.0 0.0 1.1572430210344171 0.0 40-41 0.001000210044109263 0.0 0.0 1.4853119155022556 0.0 42-43 0.001000210044109263 0.0 0.0 1.8448874263595356 0.0 44-45 0.001000210044109263 0.0 0.0 2.2649756448854257 0.0 46-47 0.001000210044109263 0.0 0.0 2.756078776543074 0.0 48-49 0.001000210044109263 0.0 0.0 3.213174766701007 0.0 50-51 0.001000210044109263 0.0 0.0 3.69727642804989 0.0 52-53 0.001000210044109263 0.0 0.0 4.166374938737135 0.0 54-55 0.001000210044109263 0.0 0.0 4.71649046299723 0.0 56-57 0.002000420088218526 0.0 0.0 5.341121635543464 0.0 58-59 0.002000420088218526 0.0 0.0 5.953750287560387 0.0 60-61 0.002000420088218526 0.0 0.0 6.598385660988807 0.0 62-63 0.002000420088218526 0.0 0.0 7.1980115824323105 0.0 64-65 0.002000420088218526 0.0 0.0 7.8326448554196375 0.0 66-67 0.002000420088218526 0.0 0.0 8.590303963832405 0.0 68-69 0.002000420088218526 0.0 0.0 9.410976305024054 0.0 70-71 0.002000420088218526 0.0 0.0 10.236149591414197 0.0 72-73 0.002000420088218526 0.0 0.0 11.072825293311595 0.0 74-75 0.002000420088218526 0.0 0.0 11.918002780583922 0.0 76-77 0.002000420088218526 0.0 0.0 12.865701797377449 0.0 78-79 0.002000420088218526 0.0 0.0 13.907420558317247 0.0 80-81 0.002000420088218526 0.0 0.0 14.94763900419088 0.0 82-83 0.002000420088218526 0.0 0.0 15.981356084777804 0.0 84-85 0.002000420088218526 0.0 0.0 17.052581042018822 0.0 86-87 0.002000420088218526 0.0 0.0 18.16531471609038 0.0 88-89 0.002000420088218526 0.0 0.0 19.315056161793976 0.001000210044109263 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACG 20 0.0020782752 71.165535 2 GCGTATG 20 0.0020782752 71.165535 8 TACATCG 25 0.005031466 56.932434 5 GGTATCA 680 0.0 43.300827 1 GCCGTAT 30 0.003937092 31.676693 92-93 ATGGGGA 75 3.0798675E-4 31.62913 5 GTATCAA 960 0.0 30.64072 2 ATCAACG 1170 0.0 25.546604 4 TATCAAC 1155 0.0 25.467611 3 TCAACGC 1230 0.0 24.300428 5 CAACGCA 1235 0.0 24.202047 6 AACGCAG 1265 0.0 23.628086 7 GTACATG 415 0.0 22.88734 1 TACATGG 395 0.0 21.619911 2 GAGTACT 825 0.0 21.576115 12-13 CATGGGG 165 6.306391E-5 20.127628 4 ACGCAGA 1520 0.0 19.664162 8 CGCAGAG 1500 0.0 19.61006 9 GTACTTT 970 0.0 18.840176 14-15 AGTACAT 330 5.857146E-10 18.68994 7 >>END_MODULE