FastQCFastQC Report
Thu 26 May 2016
SRR938822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99979
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT19311.9314055951749867No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT10441.0442192860500705TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.8021684553756289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.7801638344052251No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG5810.5811220356274818TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT5660.5661188849658428RNA PCR Primer, Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.4861020814371018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.3290691045119475No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3040.30406385340921593No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT2410.24105062063033236TruSeq Adapter, Index 11 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.21704557957171006No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.20104221886596188No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.1980415887336341No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1620.16203402714570062No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1590.15903339701337282No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1490.14903129657228018TruSeq Adapter, Index 11 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1110.11102331489612818No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA1060.10602226467558189RNA PCR Primer, Index 11 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.10402184458736334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA600.00393567431.663661
GTACAAA1354.163776E-728.145481
GGTATCA9950.027.2084731
GTACATG3550.025.4201241
GTATCAA12950.022.3723951
ACATGGG3950.020.4206473
GAGTACT8550.020.2555712-13
GAGTAAA2354.2036845E-920.210851
TACATGG4000.020.1653882
CATGGGC1250.00603159718.9791894
TATCAAC15150.018.7912772
ATCAACG15300.018.607053
GTACTTT9000.018.4519914-15
CAACGCA15750.018.3766755
TCAACGC15650.018.1909164
AACGCAG16050.018.0331866
CATGGGA1851.516501E-417.9532874
AGTACTT8650.017.82727212-13
CATGGGG1600.001189426317.7929924
AGAGTAC11150.017.02169610-11
Produced by FastQC (version 0.11.5)