##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938822_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99979 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84814811110333 31.0 31.0 34.0 30.0 34.0 2 32.05125076266016 33.0 31.0 34.0 30.0 34.0 3 32.10793266585983 33.0 31.0 34.0 30.0 34.0 4 35.79042598945779 37.0 35.0 37.0 35.0 37.0 5 35.68552396003161 37.0 35.0 37.0 33.0 37.0 6 35.724042048830256 37.0 35.0 37.0 33.0 37.0 7 35.63174266595985 37.0 35.0 37.0 33.0 37.0 8 35.640824573160366 37.0 35.0 37.0 33.0 37.0 9 37.21737564888627 39.0 37.0 39.0 33.0 39.0 10-11 37.16802528530991 39.0 37.0 39.0 33.0 39.0 12-13 37.0864831614639 39.0 37.0 39.0 33.0 39.0 14-15 38.20698846757819 40.0 37.5 41.0 33.0 41.0 16-17 38.12288580601926 40.0 37.0 41.0 33.0 41.0 18-19 38.25186789225737 40.0 37.5 41.0 33.0 41.0 20-21 38.26520569319557 40.0 37.5 41.0 33.0 41.0 22-23 38.13686374138569 40.0 37.0 41.0 33.0 41.0 24-25 37.979285649986494 40.0 37.0 41.0 33.0 41.0 26-27 37.66744516348433 40.0 36.5 41.0 32.0 41.0 28-29 37.334785304914035 39.5 36.0 41.0 32.0 41.0 30-31 36.92647956070775 39.0 35.5 41.0 31.0 41.0 32-33 36.52216965562768 39.0 35.0 41.0 30.0 41.0 34-35 36.20892387401354 39.0 35.0 41.0 30.0 41.0 36-37 35.86673201372288 39.0 35.0 41.0 29.5 41.0 38-39 35.440817571690054 39.0 35.0 41.0 27.0 41.0 40-41 35.161974014543055 39.0 35.0 41.0 26.0 41.0 42-43 34.79636223606958 38.5 35.0 41.0 24.5 41.0 44-45 34.28935576471059 38.0 34.0 41.0 21.0 41.0 46-47 33.64374018543894 38.0 33.0 40.0 11.5 41.0 48-49 33.11085327918863 38.0 33.0 40.0 2.0 41.0 50-51 32.61358385260905 38.0 33.0 40.0 2.0 41.0 52-53 32.33239480290861 38.0 32.5 40.0 2.0 41.0 54-55 31.927064683583552 37.0 31.5 40.0 2.0 41.0 56-57 31.43922723771992 37.0 31.0 40.0 2.0 41.0 58-59 30.9309454985547 36.5 30.5 40.0 2.0 41.0 60-61 30.28565498754738 36.0 29.5 39.5 2.0 41.0 62-63 29.739135218395862 35.0 28.0 39.0 2.0 41.0 64-65 29.146115684293704 35.0 27.5 39.0 2.0 40.0 66-67 28.507006471358984 35.0 26.0 38.0 2.0 40.0 68-69 28.050775662889208 35.0 25.5 38.0 2.0 40.0 70-71 27.61297872553236 34.5 24.0 37.0 2.0 39.5 72-73 27.195406035267407 34.0 22.5 37.0 2.0 39.0 74-75 26.74259094409826 34.0 20.5 36.0 2.0 39.0 76-77 25.031716660498702 31.5 18.0 34.5 2.0 36.5 78-79 25.812915712299585 33.0 18.0 35.0 2.0 37.0 80-81 25.843037037777933 33.0 18.0 35.0 2.0 37.0 82-83 25.55548665219696 33.0 15.0 35.0 2.0 36.5 84-85 25.213724882225268 33.0 8.5 35.0 2.0 36.0 86-87 24.74405125076266 33.0 3.5 35.0 2.0 35.5 88-89 24.210519209033897 32.0 2.0 35.0 2.0 35.0 90-91 23.73590954100361 32.0 2.0 35.0 2.0 35.0 92-93 23.46732813890917 32.0 2.0 34.5 2.0 35.0 94-95 23.18664919633123 31.5 2.0 34.0 2.0 35.0 96-97 22.67164104461937 31.0 2.0 34.0 2.0 35.0 98-99 22.017063583352503 31.0 2.0 34.0 2.0 35.0 100-101 19.2066984066654 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2542585532614048 1101 2 -0.17688776146581375 1101 3 -0.23384113389018069 1101 4 0.05666746449455218 1101 5 -0.38824043901525584 1101 6 -0.04807864755051128 1101 7 0.09706351704820548 1101 8 -0.03705212523478707 1101 9 0.11128699484178384 1101 10-11 -0.3638268074425568 1101 12-13 -0.07624827349618357 1101 14-15 -0.061253076299600195 1101 16-17 -0.02028663612577475 1101 18-19 0.07069163985144655 1101 20-21 0.10172216357584318 1101 22-23 -0.7124868926624188 1101 24-25 -0.22558094087776936 1101 26-27 -0.3791876042461624 1101 28-29 0.2946199537820462 1101 30-31 0.13944904962369975 1101 32-33 0.7893374917071299 1101 34-35 0.30920673577155355 1101 36-37 0.06907697710247618 1101 38-39 0.07001893184438046 1101 40-41 -0.04950816607880171 1101 42-43 -0.004502347423219533 1101 44-45 0.6926328608465511 1101 46-47 0.42317634355214295 1101 48-49 0.44573367437075717 1101 50-51 0.10920145186921104 1101 52-53 0.2884470966232584 1101 54-55 0.9105935704653092 1101 56-57 0.3212636932869657 1101 58-59 0.3984535548958874 1101 60-61 0.7847078909621636 1101 62-63 0.8891459754176978 1101 64-65 0.855100075156944 1101 66-67 0.2751584038135739 1101 68-69 -0.05819479406902062 1101 70-71 0.1797067005328259 1101 72-73 0.38714266042477163 1101 74-75 0.34618143763685083 1101 76-77 0.4366575397702839 1101 78-79 -0.08947279676187136 1101 80-81 -0.12046425825025864 1101 82-83 0.22152491591483425 1101 84-85 0.22136079678898923 1101 86-87 0.15549191303395915 1101 88-89 -0.36696610795278417 1101 90-91 -0.21650146925355074 1101 92-93 -0.45714981579704883 1101 94-95 -0.8931369119426407 1101 96-97 -1.2903218748738254 1101 98-99 -0.595317505352007 1101 100-101 -0.06575238182496079 1102 1 -0.1741050444894725 1102 2 -0.9462956562026541 1102 3 -0.6348279759954423 1102 4 -0.7552842898914136 1102 5 -0.2691614916468339 1102 6 -0.7146356650943702 1102 7 -0.46313385137284513 1102 8 -0.4198372129540857 1102 9 -0.40088405778979563 1102 10-11 -0.4182675969162446 1102 12-13 -0.2914895015663532 1102 14-15 -0.6751236903346935 1102 16-17 -0.4868436536696237 1102 18-19 -0.9359969566397908 1102 20-21 -0.64608485396802 1102 22-23 -0.860184261083468 1102 24-25 -1.0700984847374215 1102 26-27 -2.723211727053183 1102 28-29 -2.3033515374460265 1102 30-31 -2.07990402055173 1102 32-33 -1.2276032977665494 1102 34-35 -1.584269141421423 1102 36-37 -1.4201774088624362 1102 38-39 -1.170770541839829 1102 40-41 -0.6001112362542358 1102 42-43 -1.1478685754933977 1102 44-45 -0.8743079286271325 1102 46-47 -0.3073061125882077 1102 48-49 -0.705143518611699 1102 50-51 0.33266636414990813 1102 52-53 0.5160238510092299 1102 54-55 0.2254510266056613 1102 56-57 0.5035553599536335 1102 58-59 -0.013333725805868113 1102 60-61 -0.3878798283360787 1102 62-63 0.06469422103172917 1102 64-65 -0.30783852133428624 1102 66-67 0.3527351581995397 1102 68-69 0.8909828375099274 1102 70-71 1.0472505601819506 1102 72-73 0.7999167832317902 1102 74-75 0.8483744200929877 1102 76-77 0.7739930660860743 1102 78-79 0.529715799729356 1102 80-81 1.2248208294690386 1102 82-83 1.2409876352130773 1102 84-85 0.77421167398197 1102 86-87 1.7186498077708023 1102 88-89 1.2114330148542365 1102 90-91 1.1220950219745234 1102 92-93 0.9232778157818977 1102 94-95 1.3584530003380664 1102 96-97 1.2041956689858218 1102 98-99 0.38406845956027524 1102 100-101 0.37448884624521384 1103 1 0.2279323069536474 1103 2 0.3469697654159063 1103 3 0.107220694066811 1103 4 0.2566002601934727 1103 5 -0.335149041165792 1103 6 -0.15526950776556703 1103 7 0.1545231944675578 1103 8 -0.0703182542670433 1103 9 0.11162301634715988 1103 10-11 0.1682632463208833 1103 12-13 -0.04231010145316105 1103 14-15 -0.39945872146089556 1103 16-17 -0.3264022275236158 1103 18-19 0.08782873662563873 1103 20-21 -0.329729449327381 1103 22-23 0.07145127938058948 1103 24-25 0.14337067202545484 1103 26-27 0.19557718070007013 1103 28-29 0.02664280324440682 1103 30-31 -0.4789985310214533 1103 32-33 -0.6227928846369579 1103 34-35 -0.47725159756178215 1103 36-37 0.12888880505946787 1103 38-39 0.4557716200164279 1103 40-41 1.096493178007222 1103 42-43 1.3464721149423724 1103 44-45 1.8692121619218156 1103 46-47 1.3190096768854715 1103 48-49 1.2736906636180763 1103 50-51 1.6004648927294198 1103 52-53 1.3299457525372347 1103 54-55 0.6873072801427256 1103 56-57 0.8852757900611614 1103 58-59 1.625772103282987 1103 60-61 1.5031218694567876 1103 62-63 1.52389059907361 1103 64-65 1.9525463117160804 1103 66-67 1.8139688876845455 1103 68-69 1.8882097758234515 1103 70-71 1.0103518618231497 1103 72-73 1.0768268002097159 1103 74-75 1.890536276346527 1103 76-77 1.8467717870821119 1103 78-79 1.6575030096897443 1103 80-81 2.0075599352981257 1103 82-83 2.3295558298933265 1103 84-85 2.1521403666814614 1103 86-87 2.279147827012455 1103 88-89 2.093719375918184 1103 90-91 1.659842616767957 1103 92-93 1.993623033665319 1103 94-95 2.576789163326179 1103 96-97 2.225135114373483 1103 98-99 2.873768516153369 1103 100-101 3.358642779465363 1104 1 0.34621187684612664 1104 2 0.4652493353083784 1104 3 0.5803389736366995 1104 4 0.2888583247096008 1104 5 0.2024853674363598 1104 6 0.4576337180408814 1104 7 0.44484577511272505 1104 8 0.3705419607867242 1104 9 0.24065527441167944 1104 10-11 0.3671879775036828 1104 12-13 0.3017759200522079 1104 14-15 0.6005412785391044 1104 16-17 0.8241354068849844 1104 18-19 0.5018072312492947 1104 20-21 0.37994797002745173 1104 22-23 0.6359674084128457 1104 24-25 0.691757768799647 1104 26-27 0.3837492237108222 1104 28-29 0.2739546312013985 1104 30-31 0.4134745872581149 1104 32-33 -0.07978213194878947 1104 34-35 0.038877434696289015 1104 36-37 0.26329740721001116 1104 38-39 0.38050280281212423 1104 40-41 -0.15081864994976257 1104 42-43 -0.35782896032644373 1104 44-45 -0.23831471979860908 1104 46-47 0.0018053758102070105 1104 48-49 -0.2424383686399878 1104 50-51 0.148851989503612 1104 52-53 -0.3367209141294296 1104 54-55 -0.42559594566372283 1104 56-57 -0.31364894112163455 1104 58-59 -0.562399939727765 1104 60-61 0.3794659554782953 1104 62-63 0.4324927496112423 1104 64-65 0.18910545150102465 1104 66-67 -0.34732143489610223 1104 68-69 -0.16017732095074066 1104 70-71 0.3168034747263775 1104 72-73 0.4746762625753078 1104 74-75 0.4658050935508271 1104 76-77 0.4542986688025401 1104 78-79 0.18438473011984868 1104 80-81 -0.003192752873918181 1104 82-83 0.29729776537719843 1104 84-85 0.3241833774341494 1104 86-87 0.47269621410922724 1104 88-89 0.15285916086442342 1104 90-91 0.6490899285959131 1104 92-93 0.7893219583964992 1104 94-95 0.9585095934337069 1104 96-97 0.1982533939433786 1104 98-99 1.0081771183039088 1104 100-101 1.530685790218051 1105 1 0.12084883505474764 1105 2 0.2983055375032606 1105 3 0.1794964949758011 1105 4 0.21270268786224733 1105 5 0.1446203857270092 1105 6 0.1511266789099679 1105 7 -0.034701946869319045 1105 8 0.1110363627377211 1105 9 0.26238235566652435 1105 10-11 0.3647492234852763 1105 12-13 0.35620447564470936 1105 14-15 0.17398103022960498 1105 16-17 0.34902178311821785 1105 18-19 0.4896134611572833 1105 20-21 0.3701929539538469 1105 22-23 0.41670125166748306 1105 24-25 0.6324517051703396 1105 26-27 0.32887725829679226 1105 28-29 0.34417966168582126 1105 30-31 0.10685669567181577 1105 32-33 0.45025888345971055 1105 34-35 0.3388996051873647 1105 36-37 0.38329519016090075 1105 38-39 0.8316168699887143 1105 40-41 0.8196391706909623 1105 42-43 0.6204439584187043 1105 44-45 1.2014923969290265 1105 46-47 0.9942674088442374 1105 48-49 0.9543247396628587 1105 50-51 0.8594162285015088 1105 52-53 0.5861812031524707 1105 54-55 0.483671319606195 1105 56-57 -0.07376422767523394 1105 58-59 1.270822541150185 1105 60-61 1.288788646975913 1105 62-63 0.944021404970961 1105 64-65 0.8497306593493796 1105 66-67 0.9362945721984559 1105 68-69 1.120445669848504 1105 70-71 0.6857204462372977 1105 72-73 0.11174532365501122 1105 74-75 0.31826047543753333 1105 76-77 0.2506072820383274 1105 78-79 0.13400228914878554 1105 80-81 0.7114730269731098 1105 82-83 0.955040809385622 1105 84-85 1.061352205723697 1105 86-87 0.7276568614875671 1105 88-89 0.679962586205292 1105 90-91 0.5292029981004021 1105 92-93 0.9022251842029512 1105 94-95 1.5460276069577077 1105 96-97 1.4933980563976519 1105 98-99 1.167139539321532 1105 100-101 2.2041144566630138 1106 1 0.27115811340526363 1106 2 -0.043137761465814606 1106 3 0.29990886610981704 1106 4 0.09208413116121505 1106 5 0.3492595609847413 1106 6 0.14483801911615046 1106 7 0.05581351704820747 1106 8 0.06753120809855062 1106 9 0.17753699484178043 1106 10-11 -0.17195180744256078 1106 12-13 0.43666839317048556 1106 14-15 0.166455257033725 1106 16-17 0.3976300305408955 1106 18-19 0.051108306518109714 1106 20-21 0.11338883024250634 1106 22-23 0.06876310733758118 1106 24-25 0.2585857257888904 1106 26-27 0.2831040624205059 1106 28-29 0.5619116204487113 1106 30-31 0.592990716290366 1106 32-33 1.0337124917071279 1106 34-35 0.7744150691048901 1106 36-37 0.6682436437691521 1106 38-39 0.605018931844377 1106 40-41 0.2777835005878657 1106 42-43 0.3702893192434402 1106 44-45 0.7070078608465522 1106 46-47 1.2040096768854767 1106 48-49 1.1340670077040969 1106 50-51 0.9050347852025453 1106 52-53 1.4886554299565944 1106 54-55 1.8641352371319684 1106 56-57 1.488555359953633 1106 58-59 1.6482452215625507 1106 60-61 1.1205412242955006 1106 62-63 1.4070626420843624 1106 64-65 1.1842667418236097 1106 66-67 1.4353667371469108 1106 68-69 1.704930205930978 1106 70-71 2.073040033866164 1106 72-73 1.9186009937581048 1106 74-75 1.8128481043035123 1106 76-77 2.0197825397702864 1106 78-79 1.642610536571464 1106 80-81 2.0874524084164037 1106 82-83 2.067566582581499 1106 84-85 1.5205274634556538 1106 86-87 1.6004919130339594 1106 88-89 1.9651172253805527 1106 90-91 2.1618318640797867 1106 92-93 1.8072251842029523 1106 94-95 2.120821421390694 1106 96-97 1.8305114584595081 1106 98-99 1.7380158279813287 1106 100-101 1.3463309515083708 1107 1 -0.06271943761514365 1107 2 -0.07293367983316301 1107 3 -0.19111154205344505 1107 4 -0.0630179096551089 1107 5 -0.17012819411729652 1107 6 -0.1129170829246604 1107 7 0.33622168031351407 1107 8 -0.23369328169737003 1107 9 0.21651658667851592 1107 10-11 -0.018482419687451568 1107 12-13 0.16758676051743038 1107 14-15 0.19523076723780974 1107 16-17 0.3128341121735474 1107 18-19 -0.32960597919618095 1107 20-21 0.041348013915978754 1107 22-23 -0.5587879130705815 1107 24-25 -0.027026519109064395 1107 26-27 0.16024691956336312 1107 28-29 0.21415651840789707 1107 30-31 0.23309275710669652 1107 32-33 0.10279412436018731 1107 34-35 -0.5350747268134768 1107 36-37 -0.17624615214921846 1107 38-39 0.30216178898723456 1107 40-41 0.06900799038378835 1107 42-43 0.25814646210058356 1107 44-45 0.6285384730914458 1107 46-47 0.9057443707630242 1107 48-49 0.5392710893367436 1107 50-51 1.0228919280596784 1107 52-53 0.6839615524055702 1107 54-55 0.7797474820299328 1107 56-57 0.49845331913730817 1107 58-59 0.17640848686867727 1107 60-61 -0.12558122468409394 1107 62-63 -0.5736516436299226 1107 64-65 -0.788182237768229 1107 66-67 -0.1609597934653344 1107 68-69 -0.8678248961098376 1107 70-71 -1.3798171089909808 1107 72-73 -1.3059908429765912 1107 74-75 -1.2090906712066882 1107 76-77 -1.4010337867603262 1107 78-79 -1.9481037491428204 1107 80-81 -1.6980577956652283 1107 82-83 -1.4029436215001354 1107 84-85 -1.0035541691974075 1107 86-87 -0.8547121685986916 1107 88-89 -1.2893725705378145 1107 90-91 -1.679086503267154 1107 92-93 -1.1385911423276625 1107 94-95 -0.06458674187461355 1107 96-97 -0.2541824190915136 1107 98-99 -0.422698457732956 1107 100-101 0.4378615637532697 1108 1 0.4682367650906585 1108 2 0.34382853066901475 1108 3 0.3992347088064463 1108 4 0.09096053565559714 1108 5 0.23184383064766223 1108 6 0.05011891799255608 1108 7 0.4057011574976457 1108 8 0.2938233429300112 1108 9 0.10461564652717925 1108 10-11 -0.28745742542007946 1108 12-13 -0.07215183154861649 1108 14-15 0.22836536939328056 1108 16-17 0.5442030642487623 1108 18-19 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2076.0 30 2396.0 31 2876.0 32 3638.0 33 4301.0 34 5679.0 35 7702.0 36 11178.0 37 16911.0 38 13585.0 39 1162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.657930356838484 21.443396131826702 15.71132077363466 22.18735273770015 2 15.488252533031938 23.10985306914452 39.14121965612779 22.26067474169576 3 19.10301163244281 26.92565438742136 33.30699446883846 20.66433951129737 4 12.536632692865501 20.92439412276578 34.475239800358075 32.06373338401064 5 11.650592284296463 38.93268769009124 34.213422442772526 15.203297582839765 6 27.22653436824838 38.04913179162999 17.07109706329519 17.65323677682644 7 22.87880454895528 36.569679632722874 20.855379629722243 19.696136188599606 8 24.41512717670711 34.60726752618049 21.07542583942628 19.902179457686113 9 23.145860630732454 17.98177617299633 21.53452224967243 37.33784094659879 10-11 22.538233028936077 28.227927864851623 27.391752267976276 21.84208683823603 12-13 24.820712349593414 26.25001250262555 27.629802258474278 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19.352918817268836 27.41717680950666 32.343556195734806 20.886348177489698 40-41 19.211687155531994 27.379392435957712 32.86737419103158 20.54154621747872 42-43 20.18584460270465 27.113507241738017 32.5163039129391 20.18434424261823 44-45 19.147308144332477 27.519191818159083 32.055712535320446 21.27778750218799 46-47 20.79786755218596 26.371538022984826 30.94699886976265 21.883595555066567 48-49 20.044709388971686 26.327028676021964 31.307074485641984 22.321187449364366 50-51 19.18602906610388 26.569579611718463 31.985216895548064 22.259174426629592 52-53 19.266045869632624 27.108192720471298 32.59084407725623 21.034917332639854 54-55 18.861460906790427 27.01517318636914 31.244061252863105 22.879304653977336 56-57 20.14633146126417 27.8389853818571 29.70488655060838 22.30979660627035 58-59 18.711116445618035 28.94636820100422 31.227870131428915 21.11464522194883 60-61 19.365760467302806 30.19514293144492 29.353457760707357 21.08563884054492 62-63 18.068794446833834 31.148041088628613 28.982586343132056 21.800578121405493 64-65 18.11039373465295 31.080682346704542 28.522132258435562 22.286791660206944 66-67 18.302801158272196 30.21059949088036 28.265642425970107 23.220956924877346 68-69 18.405865231698655 29.50769661628942 28.397463467328137 23.688974684683785 70-71 19.02759093205039 28.42961236665883 27.981015918422457 24.56178078286832 72-73 18.827953870312765 28.106902449514397 27.962872203162664 25.102271477010174 74-75 18.652103462761808 28.473264118105984 27.45003900858189 25.424593410550322 76-77 19.074615215259293 27.716510217560998 27.6264435682405 25.582430998939216 78-79 19.009199857770298 27.707748013041062 27.402755450051835 25.880296679136805 80-81 19.036092759943386 27.6529453832574 27.072320548918015 26.238641307881196 82-83 19.125016253413214 27.3517438662119 27.392752478020384 26.130487402354497 84-85 18.912971724062054 27.28773042338891 27.283229478190417 26.516068374358614 86-87 18.88806018710863 27.47913924025288 27.188314971192877 26.44448560144561 88-89 19.028646839514472 27.042636573017077 27.208727024572354 26.719989562896096 90-91 18.83392988920627 26.757162435554392 27.640701007535178 26.768206667704153 92-93 18.671420998409666 26.875643885215894 28.031386591184148 26.421548525190293 94-95 18.678515880914397 26.454187650344824 28.15305290637487 26.71424356236591 96-97 18.65441742765981 26.03696776323028 28.34445233499035 26.96416247411956 98-99 18.697926564578562 26.02046429750248 28.14591064123466 27.135698496684302 100-101 18.589089599711937 25.17453839844766 29.117405829282443 27.11896617255796 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 219.0 1 162.0 2 95.0 3 90.5 4 89.5 5 78.5 6 115.5 7 205.0 8 264.5 9 293.0 10 257.0 11 201.0 12 203.5 13 211.5 14 217.5 15 235.5 16 264.0 17 275.5 18 284.5 19 315.0 20 353.0 21 375.5 22 419.5 23 482.0 24 542.5 25 649.5 26 817.5 27 1033.0 28 1182.5 29 1286.0 30 1537.0 31 1810.5 32 2029.5 33 2205.0 34 2391.0 35 2629.0 36 2754.5 37 2828.5 38 2905.5 39 2902.5 40 2856.5 41 2907.0 42 2974.0 43 3167.0 44 3396.0 45 3538.0 46 3659.5 47 3787.5 48 4026.0 49 4120.5 50 3880.5 51 3446.0 52 3067.5 53 2750.0 54 2553.5 55 2347.0 56 2069.5 57 1854.0 58 1639.5 59 1427.0 60 1205.5 61 963.5 62 739.0 63 573.0 64 433.0 65 328.5 66 265.0 67 212.5 68 156.5 69 120.0 70 100.0 71 78.5 72 62.5 73 45.0 74 40.5 75 33.0 76 15.5 77 14.0 78 10.5 79 3.0 80 2.5 81 2.5 82 1.5 83 1.5 84 1.5 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24205083067444164 2 0.0 3 0.0 4 0.0 5 0.027005671190950098 6 0.0030006301323277887 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002000420088218526 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.001050220546315E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0030006301323277887 36-37 0.012002520529311155 38-39 0.00700147030876484 40-41 0.006001260264655577 42-43 0.0030006301323277887 44-45 0.0015003150661638944 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.001050220546315E-4 58-59 0.001000210044109263 60-61 0.001000210044109263 62-63 0.0 64-65 5.001050220546315E-4 66-67 0.0025005251102731573 68-69 0.0 70-71 5.001050220546315E-4 72-73 0.0 74-75 0.001000210044109263 76-77 0.053011132337790944 78-79 0.14052951119735144 80-81 5.001050220546315E-4 82-83 0.0 84-85 0.0 86-87 0.09051900899188829 88-89 0.33457025975454846 90-91 0.3795797117394653 92-93 0.0 94-95 5.001050220546315E-4 96-97 0.0 98-99 0.0 100-101 0.001000210044109263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 99979.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.83413516838536 #Duplication Level Percentage of deduplicated Percentage of total 1 90.91619151761306 69.85466948059093 2 6.313624410945351 9.702037427859851 3 1.3473404670780285 3.1056521869592615 4 0.5024863964175063 1.544324308104702 5 0.265562758728423 1.0202142449914482 6 0.15360983102918588 0.7081487112293582 7 0.11846181884454166 0.6371337980976005 8 0.07550313728553204 0.464097460466698 9 0.06118357676586217 0.4230888486582182 >10 0.19526673435913458 2.670560817771732 >50 0.026035564581217942 1.3972934316206405 >100 0.016923116977791662 2.7675811920503306 >500 0.005207112916243589 2.7295732103741788 >1k 0.0026035564581217943 2.975624881225057 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1931 1.9314055951749867 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1044 1.0442192860500705 TruSeq Adapter, Index 11 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 802 0.8021684553756289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 780 0.7801638344052251 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 581 0.5811220356274818 TruSeq Adapter, Index 11 (96% over 28bp) ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 566 0.5661188849658428 RNA PCR Primer, Index 11 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 486 0.4861020814371018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 329 0.3290691045119475 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 304 0.30406385340921593 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 241 0.24105062063033236 TruSeq Adapter, Index 11 (96% over 26bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.21704557957171006 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 201 0.20104221886596188 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.1980415887336341 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 162 0.16203402714570062 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 159 0.15903339701337282 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 149 0.14903129657228018 TruSeq Adapter, Index 11 (95% over 24bp) TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 111 0.11102331489612818 No Hit CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA 106 0.10602226467558189 RNA PCR Primer, Index 11 (96% over 28bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.10402184458736334 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.001000210044109263 0.0 0.0 0.001000210044109263 0.0 20-21 0.001000210044109263 0.0 0.0 0.003000630132327789 0.0 22-23 0.001000210044109263 0.0 0.0 0.01300273057342042 0.0 24-25 0.001000210044109263 0.0 0.0 0.023004831014513047 0.0 26-27 0.001000210044109263 0.0 0.0 0.0445093469628622 0.0 28-29 0.001000210044109263 0.0 0.0 0.08351753868312345 0.0 30-31 0.001000210044109263 0.0 0.0 0.15753308194720891 0.0 32-33 0.001000210044109263 0.0 0.0 0.2950619630122326 0.0 34-35 0.0015003150661638946 0.0 0.0 0.5131077526280519 0.0 36-37 0.002000420088218526 0.0 0.0 0.8246731813680872 0.0 38-39 0.0025005251102731573 0.0 0.0 1.1817481671150942 0.0 40-41 0.0030006301323277887 0.0 0.0 1.5143180067814241 0.0 42-43 0.0030006301323277887 0.0 0.0 1.8833955130577422 0.0 44-45 0.0030006301323277887 0.0 0.0 2.307984676782124 0.0 46-47 0.0030006301323277887 0.0 0.0 2.8055891737264824 0.0 48-49 0.0030006301323277887 0.0 0.0 3.268686424149071 0.0 50-51 0.0030006301323277887 0.0 0.0 3.756288820652337 0.0 52-53 0.0030006301323277887 0.0 0.0 4.231888696626291 0.0 54-55 0.0030006301323277887 0.0 0.0 4.79000590123926 0.0 56-57 0.0030006301323277887 0.0 0.0 5.418637913961932 0.0 58-59 0.0030006301323277887 0.0 0.0 6.035267406155293 0.0 60-61 0.0030006301323277887 0.0 0.0 6.677902359495494 0.0 62-63 0.0030006301323277887 0.0 0.0 7.283029436181598 0.0 64-65 0.0030006301323277887 0.0 0.0 7.929165124676182 0.0 66-67 0.0030006301323277887 0.0 0.0 8.695326018463877 0.0 68-69 0.0030006301323277887 0.0 0.0 9.518998989787855 0.0 70-71 0.0030006301323277887 0.0 0.0 10.346172696266215 0.0 72-73 0.0030006301323277887 0.0 0.0 11.182348293141558 0.0 74-75 0.0030006301323277887 0.0 0.0 12.034527250722652 0.0 76-77 0.0030006301323277887 0.0 0.0 12.991728262935215 0.0 78-79 0.0030006301323277887 0.0 0.0 14.037447864051451 0.0 80-81 0.0030006301323277887 0.0 0.0 15.078666519969193 0.0 82-83 0.0030006301323277887 0.0 0.0 16.13888916672501 0.0 84-85 0.0030006301323277887 0.0 0.0 17.226117484671782 0.0 86-87 0.0030006301323277887 0.0 0.0 18.340851578831554 0.0 88-89 0.0030006301323277887 0.0 0.0 19.5065963852409 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTATA 60 0.003935674 31.66366 1 GTACAAA 135 4.163776E-7 28.14548 1 GGTATCA 995 0.0 27.208473 1 GTACATG 355 0.0 25.420124 1 GTATCAA 1295 0.0 22.372395 1 ACATGGG 395 0.0 20.420647 3 GAGTACT 855 0.0 20.25557 12-13 GAGTAAA 235 4.2036845E-9 20.21085 1 TACATGG 400 0.0 20.165388 2 CATGGGC 125 0.006031597 18.979189 4 TATCAAC 1515 0.0 18.791277 2 ATCAACG 1530 0.0 18.60705 3 GTACTTT 900 0.0 18.45199 14-15 CAACGCA 1575 0.0 18.376675 5 TCAACGC 1565 0.0 18.190916 4 AACGCAG 1605 0.0 18.033186 6 CATGGGA 185 1.516501E-4 17.953287 4 AGTACTT 865 0.0 17.827272 12-13 CATGGGG 160 0.0011894263 17.792992 4 AGAGTAC 1115 0.0 17.021696 10-11 >>END_MODULE