FastQCFastQC Report
Thu 26 May 2016
SRR938821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218402
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.9450462907848829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.7857070905944085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10860.4972481936978599No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9210.4216994349868591No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.30265290610891843No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4730.216573108304869No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.19825825770826275No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.187269347350299No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.15338687374657742No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2940.134614151885056No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.10439464840065567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.031.4281481
TAGGCAT804.5301378E-429.6332235
CCGTGAC650.0058862429.1773222
GTATAAA650.0058862429.1773221
GTATCAA25400.029.1198881
TAGTAGT856.459175E-427.890094
CTAGTAG700.00845204727.0932313
GTGTAGC1109.56038E-525.861721
GAGTACT16600.025.68826712-13
TATCAAC29250.025.1249182
ATCAACG29400.024.996733
TCTACAC950.001236492224.954293
TCAACGC29500.024.9119954
CCAAGAC1151.2930737E-424.7372993
CATGGGG3650.024.680824
CAACGCA29750.024.5432785
AACGCAG30100.024.2412266
GTACATG11750.023.403941
GTCCATG1252.2758037E-422.7583121
TACATGG11950.022.6154792
Produced by FastQC (version 0.11.5)