FastQCFastQC Report
Thu 26 May 2016
SRR938821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218402
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT22801.0439464840065567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.8873545114055732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.7779232790908508No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT12400.567760368494794RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.5315885385664967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10130.4638235913590535No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT6540.2994478072545123RNA PCR Primer, Index 46 (95% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.2564079083524876No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4560.20878929680131134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.19734251517843243No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.15933920019047446No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG3470.15888132892555928RNA PCR Primer, Index 46 (96% over 28bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2770.12683034038149832No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.12133588520251645No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT2340.10714187599014662RNA PCR Primer, Index 46 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG405.4036605E-447.4551283
GTGTAGA905.580059E-737.0197221
GGTATCA18550.030.7903751
CCGGCCA404.326653E-429.87489984-85
GTACACT804.4287095E-429.747991
TAGACTC650.00586085329.2031565
ATATAGG650.00586085329.2031564
GTACATG11350.027.6774251
CATGGGG4100.026.6211684
TACATGG11800.026.1405372
ACATGGG11750.025.4440273
GACATAT950.001208932825.0509381
GTATCAA25100.024.2724631
ATGGGAG2351.7644197E-1024.2324075
ATGGGAT1801.9537583E-723.7275645
CGGCTGC550.00288267621.58039532-33
CATGGGA6900.021.320424
CTTACAC1150.003734160420.6326643
GAGTGAT704.8685708E-420.34256710-11
GAGTACT19200.019.65388912-13
Produced by FastQC (version 0.11.5)