##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938821_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 218402 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.838202031116932 31.0 31.0 34.0 30.0 34.0 2 32.039894323312055 33.0 31.0 34.0 30.0 34.0 3 32.09193139256966 33.0 31.0 34.0 30.0 34.0 4 35.791274805175775 37.0 35.0 37.0 35.0 37.0 5 35.70409611633593 37.0 35.0 37.0 33.0 37.0 6 35.74619280043223 37.0 35.0 37.0 33.0 37.0 7 35.674874772209044 37.0 35.0 37.0 33.0 37.0 8 35.65536029889836 37.0 35.0 37.0 33.0 37.0 9 37.24053351159788 39.0 37.0 39.0 33.0 39.0 10-11 37.178157709178485 39.0 37.0 39.0 33.0 39.0 12-13 37.113055283376525 39.0 37.0 39.0 33.0 39.0 14-15 38.22292378274925 40.0 37.5 41.0 33.0 41.0 16-17 38.164471937070175 40.0 37.0 41.0 33.0 41.0 18-19 38.21991785789508 40.0 37.5 41.0 33.0 41.0 20-21 38.3002193203359 40.0 38.0 41.0 33.0 41.0 22-23 38.21328559262278 40.0 37.0 41.0 33.0 41.0 24-25 38.08222452175346 40.0 37.0 41.0 33.0 41.0 26-27 37.85573621120686 40.0 37.0 41.0 33.0 41.0 28-29 37.59849039843958 40.0 36.0 41.0 32.0 41.0 30-31 37.22448741311892 39.0 36.0 41.0 31.5 41.0 32-33 36.77719526377963 39.0 35.0 41.0 31.0 41.0 34-35 36.511604747209276 39.0 35.0 41.0 30.0 41.0 36-37 36.24304035677329 39.0 35.0 41.0 30.0 41.0 38-39 35.97599609893682 39.0 35.0 41.0 29.5 41.0 40-41 35.70869085447936 39.0 35.0 41.0 28.5 41.0 42-43 35.3522289173176 39.0 35.0 41.0 26.5 41.0 44-45 34.89104037508814 39.0 35.0 41.0 24.5 41.0 46-47 34.292888801384606 38.0 34.0 41.0 21.0 41.0 48-49 33.7810299356233 38.0 33.5 40.0 12.5 41.0 50-51 33.370241572879365 38.0 33.0 40.0 7.5 41.0 52-53 33.1266449025192 38.0 33.0 40.0 2.0 41.0 54-55 32.83328907244439 38.0 33.0 40.0 2.0 41.0 56-57 32.45203340628749 37.5 32.5 40.0 2.0 41.0 58-59 32.05594271114734 37.0 32.0 40.0 2.0 41.0 60-61 31.59523722310235 36.5 31.0 40.0 2.0 41.0 62-63 31.167979230959425 36.0 31.0 39.5 2.0 41.0 64-65 30.703564985668628 35.5 30.5 39.0 2.0 41.0 66-67 30.164941255116712 35.0 29.5 39.0 2.0 40.0 68-69 29.620651367661466 35.0 29.0 38.0 2.0 40.0 70-71 29.136125127059277 35.0 28.0 37.5 2.0 40.0 72-73 28.80613272772227 35.0 28.0 37.0 2.0 39.5 74-75 28.391708867134916 34.0 27.0 36.5 2.0 39.0 76-77 26.470034615067625 32.0 24.5 34.5 2.0 36.5 78-79 27.386690598071446 34.0 26.0 35.0 2.0 37.5 80-81 27.384124229631595 34.0 26.0 35.0 2.0 37.0 82-83 27.057609362551624 34.0 25.5 35.0 2.0 37.0 84-85 26.671282772135786 34.0 24.5 35.0 2.0 36.0 86-87 26.146550855761394 33.0 22.5 35.0 2.0 36.0 88-89 25.61665415151876 33.0 20.0 35.0 2.0 35.5 90-91 25.194370930669134 33.0 17.5 35.0 2.0 35.0 92-93 24.897706980705305 33.0 13.0 35.0 2.0 35.0 94-95 24.502312249887822 32.0 4.5 35.0 2.0 35.0 96-97 23.985437404419372 32.0 2.0 35.0 2.0 35.0 98-99 23.296682264814425 32.0 2.0 34.0 2.0 35.0 100-101 20.760180767575388 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06374479506669672 1101 2 0.20858694713124493 1101 3 0.48472443847130364 1101 4 0.1735241222814281 1101 5 0.23098568181100632 1101 6 0.2353236142591726 1101 7 0.25964890320253176 1101 8 0.0422015162934386 1101 9 0.18607683861817037 1101 10-11 -0.004960964955493807 1101 12-13 0.18743902830037484 1101 14-15 0.44854029324299205 1101 16-17 0.2706697032139047 1101 18-19 0.35641891944358406 1101 20-21 0.1323665903479565 1101 22-23 0.2146470213823335 1101 24-25 -0.019907026697076446 1101 26-27 0.137967782754167 1101 28-29 0.5450589798485126 1101 30-31 -0.0956639959256762 1101 32-33 -0.3287849219218799 1101 34-35 -0.32330838237887605 1101 36-37 -0.19738403388729608 1101 38-39 -0.263817667129409 1101 40-41 -0.012311606776265194 1101 42-43 0.16890084303869912 1101 44-45 0.07097402696275878 1101 46-47 0.0644920093566057 1101 48-49 0.07742409648402315 1101 50-51 -0.08664194200886044 1101 52-53 -0.41955965879938617 1101 54-55 -0.5770272743338438 1101 56-57 -0.41706209071294964 1101 58-59 -0.13403340500361338 1101 60-61 0.1274405074890943 1101 62-63 -0.1442945412263157 1101 64-65 0.17650247710062672 1101 66-67 0.13538067434858547 1101 68-69 0.04393589412694254 1101 70-71 -0.15207969728512438 1101 72-73 -0.16728937216059947 1101 74-75 0.39403402751587535 1101 76-77 0.2399957565026689 1101 78-79 0.408083830382445 1101 80-81 0.03684950737712356 1101 82-83 0.4402250038741009 1101 84-85 0.5403446789756217 1101 86-87 0.6660939370733416 1101 88-89 0.7401852731327629 1101 90-91 0.5900049301564998 1101 92-93 0.2547016397781796 1101 94-95 0.3652873971354147 1101 96-97 0.5389481882334763 1101 98-99 0.7787449852873429 1101 100-101 1.3486932818049056 1102 1 0.49924976628595275 1102 2 0.3842803013960321 1102 3 0.5390415499312695 1102 4 0.4349840908842495 1102 5 0.42046762843581575 1102 6 -0.062216940424235645 1102 7 0.2286180293145179 1102 8 0.32799429494336607 1102 9 0.13191671302947583 1102 10-11 0.3293142836054699 1102 12-13 0.34033018476296206 1102 14-15 0.6956360546244653 1102 16-17 0.8778779711364564 1102 18-19 0.6440301177690699 1102 20-21 0.2910923883594734 1102 22-23 0.6504398000322524 1102 24-25 0.6524634599591153 1102 26-27 0.6254481595202535 1102 28-29 0.4820160703770284 1102 30-31 0.5539697037080202 1102 32-33 0.8326358002131329 1102 34-35 0.7187376668100356 1102 36-37 0.7877153904978442 1102 38-39 0.8325585756753995 1102 40-41 1.0224476815544463 1102 42-43 0.8565512878319979 1102 44-45 0.9324209134096506 1102 46-47 0.9561325117113952 1102 48-49 0.6512859489173053 1102 50-51 0.7277091830565467 1102 52-53 0.7352414924303332 1102 54-55 0.6314761270267013 1102 56-57 0.8597301646507347 1102 58-59 1.0420654960952902 1102 60-61 1.5513416063901992 1102 62-63 1.227251769605715 1102 64-65 1.4985851563261576 1102 66-67 1.523842212810127 1102 68-69 1.5240510170992074 1102 70-71 1.742608947403518 1102 72-73 1.2282757769759804 1102 74-75 1.0262422954384256 1102 76-77 0.8383997334780737 1102 78-79 0.91954379898527 1102 80-81 1.1823230814221226 1102 82-83 1.298780733858404 1102 84-85 0.9589998333450609 1102 86-87 1.2468500856866314 1102 88-89 1.7006248335723235 1102 90-91 1.6524068139869605 1102 92-93 1.7035504100555166 1102 94-95 0.8027154452777481 1102 96-97 0.9925457811167888 1102 98-99 0.8274629340052932 1102 100-101 0.7769114921659721 1103 1 -0.16958374761798112 1103 2 -0.2347157260507018 1103 3 -0.21978835256060236 1103 4 -0.05999604634941136 1103 5 0.07543332002122582 1103 6 0.030379664848425136 1103 7 0.052453348924487386 1103 8 0.06378338847893161 1103 9 -0.10715876548262315 1103 10-11 -0.4703245751883216 1103 12-13 -0.10286230349994696 1103 14-15 -0.23748059398513988 1103 16-17 -0.052782917270910445 1103 18-19 -0.32400888548120577 1103 20-21 -0.37784946790633 1103 22-23 -0.2860715759157344 1103 24-25 -0.16971061010227118 1103 26-27 0.27412999411757966 1103 28-29 0.19053900284362157 1103 30-31 -0.03479880477880215 1103 32-33 0.47821182043703203 1103 34-35 0.5082284577092722 1103 36-37 0.018087365940246514 1103 38-39 0.2362422159787556 1103 40-41 0.0664346804710263 1103 42-43 0.3916180989551492 1103 44-45 0.9104274139513606 1103 46-47 0.9082090736471073 1103 48-49 0.8903253133087858 1103 50-51 1.2698182477008189 1103 52-53 0.6594740673671424 1103 54-55 0.6920395073083867 1103 56-57 0.7492931837912096 1103 58-59 1.0196386271895506 1103 60-61 0.8211863156715218 1103 62-63 0.7116865836179898 1103 64-65 0.9971405915013136 1103 66-67 0.9018125992312171 1103 68-69 0.7430831586665612 1103 70-71 0.6864514898376086 1103 72-73 1.0214502081785817 1103 74-75 0.6040321112564122 1103 76-77 0.7296240021671316 1103 78-79 0.7391176523619407 1103 80-81 0.8760753385546636 1103 82-83 0.7237355912076282 1103 84-85 1.0293501403150849 1103 86-87 0.7895369762608482 1103 88-89 0.40282779493021437 1103 90-91 0.5671774893210149 1103 92-93 0.6892853324101615 1103 94-95 0.5762076807418168 1103 96-97 0.12497626143458973 1103 98-99 0.1895431073530709 1103 100-101 1.1231375665610628 1104 1 -0.2673075310770301 1104 2 0.1379563252142333 1104 3 -0.0455009218149911 1104 4 -0.019700560956799507 1104 5 0.13034732220176437 1104 6 -0.19450518930016614 1104 7 -0.049520262093913914 1104 8 0.037728162971070844 1104 9 0.33497905353257096 1104 10-11 0.06965940451930663 1104 12-13 0.06797631430482909 1104 14-15 -0.06688673064713413 1104 16-17 0.11150295594627835 1104 18-19 -0.289376128433382 1104 20-21 -0.29546733943248427 1104 22-23 -0.11328604757984095 1104 24-25 0.13562058719207926 1104 26-27 -0.23824123165729105 1104 28-29 -0.13787699074826065 1104 30-31 -0.7623870832648834 1104 32-33 0.030667152746850945 1104 34-35 -0.4322405808506815 1104 36-37 -0.1638097036812809 1104 38-39 -0.5287489951108384 1104 40-41 -0.439661930792127 1104 42-43 -0.050897976963234726 1104 44-45 0.26655628830331324 1104 46-47 0.10217698856155977 1104 48-49 0.28810694782361423 1104 50-51 -0.2595189127023545 1104 52-53 -0.7503339116284806 1104 54-55 -0.46741802779163066 1104 56-57 -0.5313623131716056 1104 58-59 -0.39985940364951844 1104 60-61 -0.06407946542898202 1104 62-63 -0.19298155157547825 1104 64-65 0.2613752887845422 1104 66-67 0.3948593473343678 1104 68-69 0.359875540127522 1104 70-71 0.1869521239741836 1104 72-73 0.1619615225100688 1104 74-75 0.27692889162265644 1104 76-77 0.15311598089574474 1104 78-79 -0.07779488129179768 1104 80-81 -0.1148576209718506 1104 82-83 -0.25776319614523757 1104 84-85 -0.33200757946329773 1104 86-87 -0.1571844264047506 1104 88-89 -0.3180408609227534 1104 90-91 -0.13523445469696682 1104 92-93 0.11781702811360972 1104 94-95 -0.30285023706518643 1104 96-97 -0.4599757889403264 1104 98-99 0.048887165382129893 1104 100-101 -0.23916579547529437 1105 1 -0.05972459268840424 1105 2 -0.1052756710867726 1105 3 0.014838923289623551 1105 4 0.05712292765723248 1105 5 0.07712804119378802 1105 6 0.1825610458171667 1105 7 0.07164492112189436 1105 8 -0.1703171497095468 1105 9 0.23022815768238303 1105 10-11 0.13331678516895096 1105 12-13 0.37854156687677687 1105 14-15 0.28681804336743255 1105 16-17 0.49432575099886833 1105 18-19 0.06735719719370792 1105 20-21 -0.20452987555547253 1105 22-23 -0.18689603486455297 1105 24-25 0.33614075827056666 1105 26-27 0.10124553287860749 1105 28-29 -0.06401269760296202 1105 30-31 -0.05472322937515628 1105 32-33 -0.4472891528825542 1105 34-35 -0.1296050473863417 1105 36-37 -0.0942108133795827 1105 38-39 0.06232718105376733 1105 40-41 0.0827979004412569 1105 42-43 -0.09610662336249476 1105 44-45 -0.42092492774106915 1105 46-47 -0.23711326690023782 1105 48-49 0.11826531101861804 1105 50-51 0.06123013365066754 1105 52-53 0.09909390018518138 1105 54-55 0.10627287499418259 1105 56-57 -0.12569822809473408 1105 58-59 0.04131502705213563 1105 60-61 -0.4820542660300653 1105 62-63 0.011491919699523123 1105 64-65 0.219160515925914 1105 66-67 -0.14144859394409437 1105 68-69 -0.2370496708307499 1105 70-71 -0.3309995579123033 1105 72-73 -0.3463958928176396 1105 74-75 -0.1743830954308656 1105 76-77 -0.3804870707745813 1105 78-79 -0.24368321790028347 1105 80-81 -0.48903401676423996 1105 82-83 -0.2464350259914987 1105 84-85 -0.10960554501641795 1105 86-87 -0.4520867498355692 1105 88-89 -0.2821143784351783 1105 90-91 -0.20275266068471254 1105 92-93 -0.19763783259613632 1105 94-95 -0.5087917467172502 1105 96-97 0.18010050281785084 1105 98-99 0.07142791211661148 1105 100-101 -0.005077250798379396 1106 1 -0.06977025478886034 1106 2 0.04427327645749557 1106 3 -0.12994334645088657 1106 4 -0.057745097735349304 1106 5 -0.23854185523120464 1106 6 -0.018240825215244172 1106 7 -0.14138548315474964 1106 8 -0.2353355259207035 1106 9 -0.01525574921148376 1106 10-11 -0.2731093201879986 1106 12-13 -0.21766068281694828 1106 14-15 0.038839426594961424 1106 16-17 -0.1737553270721861 1106 18-19 -0.1547053932952096 1106 20-21 -0.15249735523027397 1106 22-23 -0.009237052795278089 1106 24-25 -0.2988185913229273 1106 26-27 -0.14216687384820403 1106 28-29 -0.00860563668302916 1106 30-31 -0.3838244655929941 1106 32-33 -0.10947870628493916 1106 34-35 -0.16384048563113396 1106 36-37 0.062109489549477814 1106 38-39 0.3400752599044168 1106 40-41 0.24387374407640294 1106 42-43 0.5085709576598916 1106 44-45 0.8673699826052896 1106 46-47 1.1169684793966752 1106 48-49 1.3159153834097523 1106 50-51 1.4596375286835226 1106 52-53 1.606544618527984 1106 54-55 1.6164076338760154 1106 56-57 1.9783111270673146 1106 58-59 1.9079594195234506 1106 60-61 1.8776851258844012 1106 62-63 1.6758643442456815 1106 64-65 1.5370466947877048 1106 66-67 2.273508528229865 1106 68-69 2.0813042661332766 1106 70-71 1.94984463409126 1106 72-73 2.1883741261154235 1106 74-75 1.8097688145813855 1106 76-77 1.7795307485816956 1106 78-79 1.7989607290871312 1106 80-81 1.6877674087842607 1106 82-83 1.6108987528257366 1106 84-85 1.758753960496449 1106 86-87 1.6355399346504527 1106 88-89 1.577758658651355 1106 90-91 1.9581672635830252 1106 92-93 1.5876389242810198 1106 94-95 1.5051741737638764 1106 96-97 1.6741755668561638 1106 98-99 1.5732655332325471 1106 100-101 1.0881513938168332 1107 1 0.046378693696571105 1107 2 -0.06570164154365798 1107 3 -0.1446709816685825 1107 4 0.14188188792289225 1107 5 -0.009073722232301407 1107 6 0.10549703574044145 1107 7 0.29188996864279204 1107 8 0.1905439518592189 1107 9 0.15575203263944104 1107 10-11 0.17521533091496622 1107 12-13 0.016856006359205367 1107 14-15 -0.023865570510963607 1107 16-17 -0.08799418487654265 1107 18-19 0.13608501123240302 1107 20-21 0.047033161201653684 1107 22-23 0.21627584192463445 1107 24-25 0.19648657299632077 1107 26-27 -0.01225498240823697 1107 28-29 -0.14279433048970702 1107 30-31 -0.1028739221496906 1107 32-33 0.07210853405205597 1107 34-35 0.35329888024448763 1107 36-37 0.27865074622192765 1107 38-39 0.39117179344354724 1107 40-41 0.6392046335226738 1107 42-43 0.5042941552931808 1107 44-45 0.39399548906873605 1107 46-47 0.6359086041635393 1107 48-49 0.21426897528061062 1107 50-51 0.6008041631937786 1107 52-53 0.7392862739399106 1107 54-55 0.4150442021440668 1107 56-57 0.10844811336868077 1107 58-59 0.4820799417435353 1107 60-61 0.11226612787809742 1107 62-63 0.4299964427729215 1107 64-65 0.30699974643089334 1107 66-67 0.9158971062734658 1107 68-69 0.7430831586665612 1107 70-71 0.9258881095559204 1107 72-73 0.9698070156903178 1107 74-75 0.9937034727587601 1107 76-77 1.1005160209464755 1107 78-79 1.1311364317046646 1107 80-81 1.0685636014654598 1107 82-83 0.981951553648944 1107 84-85 0.8791153985310487 1107 86-87 1.0853116241481686 1107 88-89 0.6117479827236423 1107 90-91 0.8981634048139711 1107 92-93 0.6752008253679058 1107 94-95 0.7287898403662254 1107 96-97 0.8104222708242617 1107 98-99 0.7411862998413348 1107 100-101 2.205297190974207 1108 1 0.007855315987395528 1108 2 0.030574646320509657 1108 3 0.07096989555824962 1108 4 0.0061818429039206535 1108 5 0.003467277188889284 1108 6 -0.01367461516958457 1108 7 0.17824922004160015 1108 8 0.04776949691034815 1108 9 -0.09288131998773963 1108 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-0.585781404633078 2316 76-77 -0.6101343353747204 2316 78-79 -0.9222275770490533 2316 80-81 -0.6153579891673111 2316 82-83 -0.9044371637818038 2316 84-85 -0.6890438892006543 2316 86-87 -0.3622315365424029 2316 88-89 -1.2487162635775384 2316 90-91 -1.8612309426786844 2316 92-93 -1.9500914328293497 2316 94-95 -1.694138165918492 2316 96-97 -2.0002318264018584 2316 98-99 -1.9961554131190304 2316 100-101 -3.2076425991610265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 11.0 6 45.0 7 126.0 8 267.0 9 474.0 10 795.0 11 1177.0 12 1640.0 13 2221.0 14 2407.0 15 2937.0 16 3685.0 17 4434.0 18 4027.0 19 2561.0 20 2309.0 21 2452.0 22 2593.0 23 2716.0 24 2963.0 25 2999.0 26 2942.0 27 3276.0 28 3996.0 29 4622.0 30 5612.0 31 6606.0 32 7874.0 33 9836.0 34 12753.0 35 17700.0 36 24483.0 37 37695.0 38 34524.0 39 3640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29044117647059 21.954963235294116 15.559283088235293 21.1953125 2 15.893169476469996 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20.07467880330766 27.586972646770636 32.347460188093514 19.990888361828187 22-23 19.842767007628133 28.712420215932088 32.16843252351169 19.27638025292809 24-25 19.67610186719902 28.95234475874763 32.78564298861732 18.58591038543603 26-27 19.533932839442862 28.9136546368623 33.16613400976182 18.38627851393302 28-29 18.669700826915506 28.76026776311572 33.40147983992821 19.168551570040567 30-31 19.297900202379097 29.270336352231208 32.600891933224055 18.83087151216564 32-33 20.11932125163689 28.45647017884452 32.74237415408284 18.681834415435755 34-35 19.512228627948403 28.333737825177096 32.91380660405438 19.24022694282012 36-37 18.999335943761306 28.818208880034806 32.69973208765543 19.482723088548465 38-39 19.066328715120125 27.90046456889426 33.07574831310433 19.95745840288129 40-41 18.876266095206695 27.952085279410955 33.30585929629833 19.865789329084016 42-43 19.371418498007063 27.871096835343057 32.78211876106073 19.975365905589147 44-45 18.878978383600657 28.243948003424922 32.398042115577454 20.479031497396967 46-47 20.03786647374759 27.686253142184718 31.423678679847434 20.852201704220256 48-49 19.639701101638263 27.648556331901723 31.20690286718986 21.504839699270153 50-51 18.829268962738436 28.070942573785956 31.688812373513066 21.410976089962546 52-53 19.028671898608987 28.58673455371288 31.71262167928865 20.671971868389484 54-55 18.935266160566293 28.101619948535273 31.067481067023195 21.89563282387524 56-57 19.584985485480903 28.763930733235043 29.81451635058287 21.836567430701184 58-59 18.796116327039876 29.135559966392172 30.86883560781405 21.1994880987539 60-61 19.26753998379128 29.81968947028631 29.82838906415264 21.084381481769775 62-63 18.418787373742 30.21492477175117 29.847254146024305 21.519033708482525 64-65 18.28623364254906 30.457825477788663 29.451424437505153 21.804516442157123 66-67 18.580000595234004 30.03550799788463 28.909142107540468 22.475349299340895 68-69 18.553215064914845 29.610144618970107 29.164634858277072 22.672005457837972 70-71 18.847570621727613 29.166275563817685 29.007621354444492 22.97853246001021 72-73 18.777529509803024 28.874735579344513 28.79002939533521 23.557705515517256 74-75 18.696944167177957 29.090850816384467 28.38687374657741 23.82533126986017 76-77 18.690633600608646 28.770862025899508 28.681261013935135 23.857243359556715 78-79 18.649521366738423 28.624559153574864 28.579214949846566 24.14670452984015 80-81 18.684581643025247 28.74263971941649 28.396260107508176 24.176518530050092 82-83 18.770661440829297 28.53522403640992 28.334218551112166 24.359895971648612 84-85 18.71457221087719 28.25477788664939 28.32551899707878 24.70513090539464 86-87 18.783575262468847 28.3127019101358 28.110705794147385 24.793017033247963 88-89 19.05365587026868 27.900225757481806 28.02768801831782 25.01843035393169 90-91 18.78675744790256 27.945301703755053 28.060538168061594 25.207402680280794 92-93 18.891997326031813 27.81682402175804 28.07803957839214 25.213139073818002 94-95 18.8553676248386 27.3729178304228 28.19708610727008 25.574628437468522 96-97 18.912601532952994 27.257076400399267 28.13298413018196 25.69733793646578 98-99 18.802941365005815 27.283175062499428 27.938159906960557 25.9757236655342 100-101 18.81025368647822 26.905510314812993 28.620486768513665 25.663749230195126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 736.0 1 481.0 2 244.5 3 254.5 4 219.5 5 227.5 6 331.5 7 494.0 8 680.5 9 781.5 10 676.0 11 553.0 12 546.0 13 579.0 14 628.5 15 674.5 16 699.0 17 727.5 18 786.0 19 851.0 20 956.5 21 1070.0 22 1200.5 23 1347.5 24 1508.5 25 1763.0 26 2131.0 27 2555.5 28 2862.0 29 3156.5 30 3603.0 31 4047.0 32 4472.0 33 4956.5 34 5509.0 35 5864.0 36 6214.5 37 6642.5 38 6610.0 39 6394.5 40 6507.0 41 6777.5 42 6901.0 43 7158.5 44 7428.5 45 7462.5 46 7585.0 47 7888.5 48 8356.5 49 8445.5 50 7740.5 51 6870.0 52 6048.0 53 5409.0 54 4963.0 55 4435.5 56 3982.0 57 3580.0 58 3236.5 59 2826.0 60 2409.5 61 2073.0 62 1669.5 63 1226.0 64 880.0 65 688.0 66 527.5 67 392.5 68 307.5 69 225.0 70 159.5 71 137.5 72 120.0 73 93.5 74 71.0 75 53.5 76 35.5 77 19.0 78 12.5 79 7.5 80 5.0 81 4.0 82 3.0 83 4.0 84 2.5 85 2.5 86 2.0 87 0.0 88 0.0 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36721275446195545 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0011446781622878911 18-19 2.2893563245757821E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009615296563218285 36-37 0.021519949451012352 38-39 0.012591459785166802 40-41 0.006410197708812191 42-43 0.0025182919570333603 44-45 0.0013736137947454694 46-47 0.0013736137947454694 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.2893563245757821E-4 60-61 4.5787126491515643E-4 62-63 0.0 64-65 0.0 66-67 2.2893563245757821E-4 68-69 2.2893563245757821E-4 70-71 0.0011446781622878911 72-73 0.0 74-75 0.0 76-77 0.09729764379447074 78-79 0.03296673107389127 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1504107105246289 88-89 0.3161601084239155 90-91 0.2696861750350272 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0011446781622878911 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 218402.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.51203548106314 #Duplication Level Percentage of deduplicated Percentage of total 1 89.40694683464442 64.83079701127261 2 6.771146128622932 9.819791766523387 3 1.5387154516865107 3.34726168283857 4 0.6420826595453762 1.8623488236291883 5 0.3955386224746825 1.4340655313507507 6 0.2580719491319713 1.1227993399274774 7 0.17964380999658272 0.91184368270979 8 0.12891550066032229 0.7478340286352246 9 0.10738829834206044 0.7008249689667432 >10 0.5222910203421394 6.622341887205224 >50 0.027787536927345965 1.4012928278721846 >100 0.016419908184340797 2.369833458901488 >500 0.0012630698603339073 0.5559377428224941 >1k 0.0037892095810017224 4.273027247344887 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 2280 1.0439464840065567 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.8873545114055732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1699 0.7779232790908508 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1240 0.567760368494794 RNA PCR Primer, Index 46 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1161 0.5315885385664967 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1013 0.4638235913590535 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 654 0.2994478072545123 RNA PCR Primer, Index 46 (95% over 23bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 560 0.2564079083524876 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 456 0.20878929680131134 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.19734251517843243 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 348 0.15933920019047446 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 347 0.15888132892555928 RNA PCR Primer, Index 46 (96% over 28bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 277 0.12683034038149832 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 265 0.12133588520251645 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 234 0.10714187599014662 RNA PCR Primer, Index 46 (96% over 26bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.5787126491515643E-4 0.0 0.0 0.0 0.0 7 4.5787126491515643E-4 0.0 0.0 0.0 0.0 8 4.5787126491515643E-4 0.0 0.0 0.0 0.0 9 4.5787126491515643E-4 0.0 0.0 0.0 0.0 10-11 4.5787126491515643E-4 0.0 0.0 2.2893563245757821E-4 0.0 12-13 4.5787126491515643E-4 0.0 0.0 4.5787126491515643E-4 0.0 14-15 4.5787126491515643E-4 0.0 0.0 4.5787126491515643E-4 0.0 16-17 4.5787126491515643E-4 0.0 0.0 4.5787126491515643E-4 0.0 18-19 4.5787126491515643E-4 0.0 0.0 4.5787126491515643E-4 0.0 20-21 4.5787126491515643E-4 0.0 0.0 6.868068973727346E-4 0.0 22-23 4.5787126491515643E-4 0.0 0.0 0.0052655195465242995 0.0 24-25 4.5787126491515643E-4 0.0 0.0 0.012362524152709223 0.0 26-27 4.5787126491515643E-4 0.0 0.0 0.02953269658702759 0.0 28-29 6.868068973727346E-4 0.0 0.0 0.06318623455829159 0.0 30-31 9.157425298303129E-4 0.0 0.0 0.14148222085878334 0.0 32-33 9.157425298303129E-4 0.0 0.0 0.29898993598959717 0.0 34-35 9.157425298303129E-4 0.0 0.0 0.5647842052728456 0.0 36-37 9.157425298303129E-4 0.0 0.0 0.9123084953434493 0.0 38-39 9.157425298303129E-4 0.0 0.0 1.3349236728601386 0.0 40-41 9.157425298303129E-4 0.0 0.0 1.7946264228349558 0.0 42-43 9.157425298303129E-4 0.0 0.0 2.2495215245281637 0.0 44-45 9.157425298303129E-4 0.0 0.0 2.789809617128048 0.0 46-47 0.0013736137947454694 0.0 0.0 3.402899240849443 0.0 48-49 0.0013736137947454694 0.0 0.0 3.9795880990100825 0.0 50-51 0.0013736137947454694 0.0 0.0 4.5883279457147825 0.0 52-53 0.0013736137947454694 0.0 0.0 5.191573337240501 0.0 54-55 0.0013736137947454694 0.0 0.0 5.862583675973664 0.0 56-57 0.0013736137947454694 0.0 0.0 6.63707292057765 0.0 58-59 0.0013736137947454694 0.0 0.0 7.423008946804517 0.0 60-61 0.0013736137947454694 0.0 0.0 8.204137324749773 0.0 62-63 0.0013736137947454694 0.0 0.0 8.944515160117582 0.0 64-65 0.0013736137947454694 0.0 0.0 9.733885220831311 0.0 66-67 0.0013736137947454694 0.0 0.0 10.623300152929001 0.0 68-69 0.0013736137947454694 0.0 0.0 11.620543767914214 0.0 70-71 0.0013736137947454694 0.0 0.0 12.66838215767255 0.0 72-73 0.0013736137947454694 0.0 0.0 13.661504931273523 0.0 74-75 0.0013736137947454694 0.0 0.0 14.697209732511608 0.0 76-77 0.0013736137947454694 0.0 0.0 15.787630149907052 0.0 78-79 0.0013736137947454694 0.0 0.0 16.987252863984764 0.0 80-81 0.0013736137947454694 0.0 0.0 18.19832235968535 0.0 82-83 0.0013736137947454694 0.0 0.0 19.382606386388403 0.0 84-85 0.0013736137947454694 0.0 0.0 20.550864918819425 0.0 86-87 0.0013736137947454694 0.0 0.0 21.754150603016456 0.0 88-89 0.0013736137947454694 0.0 0.0 23.00459702749975 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 40 5.4036605E-4 47.455128 3 GTGTAGA 90 5.580059E-7 37.019722 1 GGTATCA 1855 0.0 30.790375 1 CCGGCCA 40 4.326653E-4 29.874899 84-85 GTACACT 80 4.4287095E-4 29.74799 1 TAGACTC 65 0.005860853 29.203156 5 ATATAGG 65 0.005860853 29.203156 4 GTACATG 1135 0.0 27.677425 1 CATGGGG 410 0.0 26.621168 4 TACATGG 1180 0.0 26.140537 2 ACATGGG 1175 0.0 25.444027 3 GACATAT 95 0.0012089328 25.050938 1 GTATCAA 2510 0.0 24.272463 1 ATGGGAG 235 1.7644197E-10 24.232407 5 ATGGGAT 180 1.9537583E-7 23.727564 5 CGGCTGC 55 0.002882676 21.580395 32-33 CATGGGA 690 0.0 21.32042 4 CTTACAC 115 0.0037341604 20.632664 3 GAGTGAT 70 4.8685708E-4 20.342567 10-11 GAGTACT 1920 0.0 19.653889 12-13 >>END_MODULE