FastQCFastQC Report
Thu 26 May 2016
SRR938820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218852
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.9458446804232998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18420.841664686637545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.5103905835907371No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9560.4368248862244805No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.28695191270813153No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4490.2051614789903679No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.19510902344963718No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.17043481439511635No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.14484674574598358No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2910.1329665710160291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACC405.4234546E-447.419934
TAATACC550.0025964634.4872254
AGCTCCG600.00397670931.6132895
GGTATCA21300.030.772121
GTCGGGA650.0058821329.1814982
GTTATTC856.4537826E-427.894083
CGACGAG350.00836586127.15620690-91
AAGCCGA350.00842223727.11884924-25
GGGGCGC700.00844616227.0971036
ATGGGGA1806.7557266E-926.3444085
GTACATG13400.025.874741
TACATGG13850.024.6515162
ACATGGG13600.024.407323
GTATCAA27450.024.0122782
CATGGGG4800.023.7099674
CGATACC550.002864429521.60400894-95
ATCAACG32450.021.1891844
AACGCTT905.1397965E-621.1190742-43
GAGTACT17750.021.11488312-13
TATCAAC32300.020.9939633