##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938820_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 218852 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07798877780418 33.0 31.0 34.0 28.0 34.0 2 31.10544112002632 33.0 31.0 34.0 28.0 34.0 3 31.075850346352787 33.0 31.0 34.0 27.0 34.0 4 34.62870341600716 37.0 35.0 37.0 32.0 37.0 5 34.62975435454097 37.0 35.0 37.0 32.0 37.0 6 34.70393233783562 37.0 35.0 37.0 32.0 37.0 7 34.62428947416519 37.0 35.0 37.0 32.0 37.0 8 34.611541132820356 37.0 35.0 37.0 32.0 37.0 9 36.11635260358599 39.0 37.0 39.0 32.0 39.0 10-11 36.01383583426242 39.0 37.0 39.0 32.0 39.0 12-13 35.90899786156855 39.0 37.0 39.0 31.5 39.0 14-15 36.96762652386087 40.0 37.0 41.0 31.0 41.0 16-17 36.840047155155084 40.0 37.0 41.0 31.0 41.0 18-19 36.83943029992872 40.0 37.0 41.0 31.0 41.0 20-21 36.74905187067059 40.0 37.0 41.0 31.0 41.0 22-23 36.66225120172537 40.0 36.5 41.0 30.5 41.0 24-25 36.5502371465648 40.0 36.0 41.0 30.0 41.0 26-27 36.359261053131796 40.0 36.0 41.0 30.0 41.0 28-29 36.1166381847093 39.5 35.5 41.0 29.5 41.0 30-31 35.772510646464276 39.0 35.0 41.0 27.5 41.0 32-33 35.42600250397528 39.0 35.0 41.0 27.0 41.0 34-35 35.02050015535613 38.0 35.0 41.0 25.5 41.0 36-37 34.70475024217279 38.0 35.0 40.0 24.0 41.0 38-39 34.446712847038185 38.0 35.0 40.0 23.0 41.0 40-41 34.17547931935738 38.0 34.0 40.0 19.5 41.0 42-43 33.93196543783013 38.0 34.0 41.0 15.0 41.0 44-45 33.550972803538464 38.0 33.5 41.0 5.5 41.0 46-47 32.96265741231517 38.0 33.0 40.5 2.0 41.0 48-49 32.4547662347157 38.0 33.0 40.0 2.0 41.0 50-51 31.273276003874766 37.0 31.5 39.5 2.0 40.5 52-53 31.28824273938552 37.5 31.0 39.5 2.0 40.5 54-55 31.70610046972383 38.0 31.5 40.0 2.0 41.0 56-57 31.57624102132948 38.0 31.0 40.0 2.0 41.0 58-59 31.357673222086156 37.5 31.0 40.0 2.0 41.0 60-61 31.039768884908522 37.0 31.0 40.0 2.0 41.0 62-63 30.650779979164 36.0 30.0 40.0 2.0 41.0 64-65 30.24201515179208 36.0 29.0 39.5 2.0 41.0 66-67 29.82641465465246 35.0 29.0 39.0 2.0 41.0 68-69 29.375050262277703 35.0 28.0 39.0 2.0 40.5 70-71 28.937352640140368 35.0 27.5 38.0 2.0 40.0 72-73 28.472773838027525 35.0 26.0 37.0 2.0 40.0 74-75 27.975355491382302 35.0 26.0 37.0 2.0 39.0 76-77 27.4746266883556 34.0 25.0 36.0 2.0 39.0 78-79 26.97764242501782 34.0 23.5 36.0 2.0 38.5 80-81 26.4225572533036 34.0 20.5 35.0 2.0 37.0 82-83 25.951291283607187 34.0 18.5 35.0 2.0 37.0 84-85 25.548681300604976 33.5 13.5 35.0 2.0 36.5 86-87 25.046853581415753 33.0 4.5 35.0 2.0 36.0 88-89 24.520411054045656 33.0 2.0 35.0 2.0 35.5 90-91 23.98943806773527 32.5 2.0 35.0 2.0 35.0 92-93 23.46828678741798 32.0 2.0 35.0 2.0 35.0 94-95 22.878525213386215 32.0 2.0 35.0 2.0 35.0 96-97 22.246652989234732 31.0 2.0 35.0 2.0 35.0 98-99 21.49602242611445 31.0 2.0 34.0 2.0 35.0 100-101 19.44081616800395 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41839980645563557 1101 2 0.6356875367268096 1101 3 0.7526234745824816 1101 4 0.4603929496978836 1101 5 0.4300802598057132 1101 6 0.730816099830939 1101 7 0.6027202343605822 1101 8 0.542483258061111 1101 9 0.534370607440465 1101 10-11 0.6830524517310295 1101 12-13 0.5406601447388297 1101 14-15 0.9758586812555095 1101 16-17 0.8935259310037438 1101 18-19 0.9280885887772428 1101 20-21 1.1942828735427113 1101 22-23 0.9467740422941802 1101 24-25 0.7419512075631829 1101 26-27 0.8919135855557982 1101 28-29 0.8786356524537098 1101 30-31 1.2986350196227718 1101 32-33 0.7263688301504629 1101 34-35 1.2609070179973045 1101 36-37 1.1502531920914834 1101 38-39 1.0449077268107558 1101 40-41 0.7537276403047315 1101 42-43 0.6067803725572105 1101 44-45 0.5183088579855735 1101 46-47 0.5866396168203494 1101 48-49 0.3893717484682284 1101 50-51 0.56278258317192 1101 52-53 0.6606295592379574 1101 54-55 0.4997574360599195 1101 56-57 0.5235321235009103 1101 58-59 0.4300283392946369 1101 60-61 0.4930378258526851 1101 62-63 0.26076379493689217 1101 64-65 0.6064072948699746 1101 66-67 0.2962113221842486 1101 68-69 0.003929869263561869 1101 70-71 0.011255987108171439 1101 72-73 -0.3504723773356737 1101 74-75 -0.07877056991492637 1101 76-77 -0.6054647917010669 1101 78-79 -0.3214684413668003 1101 80-81 -0.2696034972568313 1101 82-83 0.26443661461512136 1101 84-85 -0.048629154783778716 1101 86-87 -0.1077446601068921 1101 88-89 -0.23184317902730456 1101 90-91 -0.3767791976051882 1101 92-93 -0.15500013822705938 1101 94-95 -0.18114443222287235 1101 96-97 -0.11735651467942887 1101 98-99 0.48591993940163647 1101 100-101 0.8754027899526733 1102 1 0.14442285336607696 1102 2 0.2780067455100408 1102 3 -0.024483865949232353 1102 4 0.2615906631540099 1102 5 0.27442287844286284 1102 6 0.04707596735656949 1102 7 0.3854531987013914 1102 8 0.3512574164859359 1102 9 0.3979945980039332 1102 10-11 0.3004646647788505 1102 12-13 0.33400920562259984 1102 14-15 0.45519358734387794 1102 16-17 0.502408972470036 1102 18-19 0.3347213807199054 1102 20-21 0.5130606614022568 1102 22-23 0.06686296344471288 1102 24-25 0.02679378083239925 1102 26-27 -0.2232982826957226 1102 28-29 0.07625837271503144 1102 30-31 -0.04173790479425321 1102 32-33 0.07812910598750022 1102 34-35 0.1395024267623839 1102 36-37 0.17611291443972732 1102 38-39 0.29987551557764647 1102 40-41 0.5905845498410685 1102 42-43 0.5077648567237958 1102 44-45 0.552992643485986 1102 46-47 0.3346207437598352 1102 48-49 0.34413991832577295 1102 50-51 0.44580136549656757 1102 52-53 0.2732781392216239 1102 54-55 -0.11602153136590232 1102 56-57 0.03922942864563694 1102 58-59 0.02319139164923456 1102 60-61 -0.1737800672604628 1102 62-63 -0.3991808561836976 1102 64-65 -0.44263544172380165 1102 66-67 -0.6869344903554513 1102 68-69 -0.02090907457093394 1102 70-71 -0.2532163838200532 1102 72-73 -0.24406188826934638 1102 74-75 -0.42451959450434984 1102 76-77 -0.5313335876361016 1102 78-79 -0.5535027395248626 1102 80-81 -0.22954361158402392 1102 82-83 -0.7787309745328663 1102 84-85 -0.9316706210754013 1102 86-87 -0.7255198166262637 1102 88-89 -0.7256277720769368 1102 90-91 -0.830617324816874 1102 92-93 -0.7719133040014903 1102 94-95 -0.9455260672832111 1102 96-97 -0.9908026629418387 1102 98-99 -1.252851496947148 1102 100-101 -1.4054247211624684 1103 1 0.3856895260818014 1103 2 0.45811744326886483 1103 3 0.40682908205911517 1103 4 -0.05362574189090452 1103 5 0.2992391383103836 1103 6 0.20745161384962785 1103 7 0.0606641595942321 1103 8 0.1499598935751294 1103 9 0.08577247659933818 1103 10-11 0.2624917040674788 1103 12-13 0.4355199578229403 1103 14-15 0.4034287747134542 1103 16-17 0.49165677212523917 1103 18-19 0.11266802802958154 1103 20-21 0.2947501632623428 1103 22-23 0.3322880609857748 1103 24-25 0.6064371888716025 1103 26-27 0.7306986322847706 1103 28-29 1.0141496711452973 1103 30-31 0.6631210009311772 1103 32-33 0.8688921946364445 1103 34-35 0.9384771114552493 1103 36-37 1.019412070596161 1103 38-39 1.379019876343456 1103 40-41 1.1999893225477223 1103 42-43 1.3334158865758994 1103 44-45 0.7566266149949215 1103 46-47 0.6450508317736237 1103 48-49 0.557596047533643 1103 50-51 0.45296949905976547 1103 52-53 0.7190407741912317 1103 54-55 0.9086359407328182 1103 56-57 0.5398872636878345 1103 58-59 0.4510563766778155 1103 60-61 -0.020980865736103027 1103 62-63 -0.08970349478273576 1103 64-65 -0.12490111634497936 1103 66-67 -0.09864849089986549 1103 68-69 -0.41429442980185627 1103 70-71 -0.6873421437329483 1103 72-73 -0.8761733119151138 1103 74-75 -0.4619481400083849 1103 76-77 -0.9886423617945255 1103 78-79 -1.108851618936896 1103 80-81 -0.4728745252942126 1103 82-83 -0.7192082451979651 1103 84-85 -0.7168534538491969 1103 86-87 -0.8951278376769842 1103 88-89 -0.8439927117375845 1103 90-91 -0.825377328446308 1103 92-93 -1.1783646242083705 1103 94-95 -1.0806771425032444 1103 96-97 -0.7318424959878413 1103 98-99 -1.2594071634020985 1103 100-101 -1.2694570231314373 1104 1 0.37004547894649065 1104 2 0.22761042263588038 1104 3 0.6079442565590938 1104 4 0.5227885431784003 1104 5 0.28427232265279656 1104 6 0.6082597767463795 1104 7 0.9455348083819857 1104 8 0.7425148621967352 1104 9 0.6028161354552708 1104 10-11 0.9652435213052755 1104 12-13 0.8033469477547683 1104 14-15 0.9406539316054108 1104 16-17 1.140512250927891 1104 18-19 1.0025840513263518 1104 20-21 0.8205978313286195 1104 22-23 0.9589529217018296 1104 24-25 0.8906486656877064 1104 26-27 1.075104700278814 1104 28-29 1.041058335193334 1104 30-31 1.1547233306074602 1104 32-33 1.309544142805656 1104 34-35 1.24939408287608 1104 36-37 1.81890640442613 1104 38-39 1.4432552820051185 1104 40-41 1.310942412980694 1104 42-43 1.543809132320625 1104 44-45 2.353764183282429 1104 46-47 2.332768110206054 1104 48-49 2.6658063504818585 1104 50-51 2.5288532861381903 1104 52-53 3.1735759676536865 1104 54-55 2.8397614994487874 1104 56-57 3.1301960812416674 1104 58-59 3.2795700793280425 1104 60-61 3.2928256266563345 1104 62-63 3.1163554695045903 1104 64-65 3.7321804674736967 1104 66-67 2.7761652704437623 1104 68-69 2.39745779377483 1104 70-71 2.4606103597660827 1104 72-73 2.8366918287232927 1104 74-75 2.929943141765058 1104 76-77 3.159569747104296 1104 78-79 3.0987649777491875 1104 80-81 2.7912457510162305 1104 82-83 3.010361938557512 1104 84-85 2.775437394496098 1104 86-87 2.777622625097859 1104 88-89 2.69808593741136 1104 90-91 2.622656442830049 1104 92-93 2.9104655712857834 1104 94-95 3.088337259952848 1104 96-97 3.273338324816713 1104 98-99 3.431989739392609 1104 100-101 4.0366628919888825 1105 1 -0.029422909130211394 1105 2 0.3394176922179746 1105 3 0.3815872749334588 1105 4 0.5260583784208919 1105 5 0.40496088666773034 1105 6 0.019984773054623872 1105 7 0.042870420052132374 1105 8 0.1625093773118138 1105 9 0.07552883296386881 1105 10-11 0.27213338059687686 1105 12-13 0.4192362374621652 1105 14-15 0.48041934729639735 1105 16-17 0.28411075735153446 1105 18-19 0.08666100848095937 1105 20-21 0.24967405013333632 1105 22-23 0.11813061087762122 1105 24-25 0.5368946838722124 1105 26-27 0.33651220613287336 1105 28-29 0.09116064939286161 1105 30-31 0.26329240851392655 1105 32-33 0.15353081031615545 1105 34-35 0.43511017679418273 1105 36-37 0.6273784420608806 1105 38-39 0.44891948047357033 1105 40-41 0.32474955598851807 1105 42-43 0.3466591629094111 1105 44-45 0.6533127350618528 1105 46-47 1.0428286548973205 1105 48-49 1.1255979248547945 1105 50-51 1.417024920844856 1105 52-53 0.9820901967109172 1105 54-55 0.7943846245674528 1105 56-57 0.8518592671159801 1105 58-59 1.3577718809017831 1105 60-61 1.2607601455686357 1105 62-63 0.8539564652221614 1105 64-65 0.881812347312529 1105 66-67 0.8013535496665938 1105 68-69 0.6203339830455512 1105 70-71 1.067871503575546 1105 72-73 0.7798177912151161 1105 74-75 0.7352930141360048 1105 76-77 0.3546523368138068 1105 78-79 0.33272696327752627 1105 80-81 0.9952518265810646 1105 82-83 0.21852386923700706 1105 84-85 0.39165366772775556 1105 86-87 0.5385027381708731 1105 88-89 0.3521057251885047 1105 90-91 0.21757659556299558 1105 92-93 0.5176317843946592 1105 94-95 0.8349250286594696 1105 96-97 1.4549407550426459 1105 98-99 1.421948180841465 1105 100-101 1.8665365286785445 1106 1 0.19089894110430095 1106 2 0.2070790216696956 1106 3 -0.13038102524444994 1106 4 0.1952925689432945 1106 5 0.1373319039732479 1106 6 -0.037875488954107084 1106 7 0.03993899517747934 1106 8 -0.14919206211888536 1106 9 -0.03265085327258532 1106 10-11 0.09718796574971833 1106 12-13 0.23692182698182052 1106 14-15 -0.2744225579276005 1106 16-17 0.052750576209696476 1106 18-19 0.04757197403166202 1106 20-21 0.3144585398632458 1106 22-23 0.1322621004457929 1106 24-25 0.009106797732790994 1106 26-27 0.20352660043984372 1106 28-29 -0.28885309797896497 1106 30-31 -0.10342884330557922 1106 32-33 -0.2828471615421648 1106 34-35 -0.1268136465925167 1106 36-37 0.22510608236493823 1106 38-39 0.2665242031001256 1106 40-41 0.22549555307730884 1106 42-43 -0.5102367613991845 1106 44-45 -0.4465578779091999 1106 46-47 -0.31507810557494054 1106 48-49 -0.24914936610429805 1106 50-51 0.17458597534914233 1106 52-53 -0.20766396793407793 1106 54-55 -0.3823600786510504 1106 56-57 -0.09826607656831854 1106 58-59 -0.0453091477250922 1106 60-61 0.11498746240512503 1106 62-63 -0.06973551278204582 1106 64-65 0.11462813253006132 1106 66-67 0.09138266174810639 1106 68-69 0.425940080409287 1106 70-71 0.7126232422137448 1106 72-73 0.3353834551323054 1106 74-75 0.6136186651144939 1106 76-77 0.29584188881121776 1106 78-79 0.49137769916625373 1106 80-81 0.45579889111284544 1106 82-83 0.13294647962031192 1106 84-85 0.04848489852186688 1106 86-87 0.07311376841508732 1106 88-89 0.21459157348221325 1106 90-91 0.42992294846611756 1106 92-93 0.4920879545386043 1106 94-95 0.4160345224327209 1106 96-97 0.582215136410916 1106 98-99 0.5810436846422071 1106 100-101 0.798840830797257 1107 1 0.025876441969653996 1107 2 0.28989314420344314 1107 3 0.04234310075070624 1107 4 0.3435705197913421 1107 5 0.33662231588048286 1107 6 0.20745161384962785 1107 7 0.48589780445404074 1107 8 0.46304400572466164 1107 9 0.10446406538439135 1107 10-11 0.37697768537588416 1107 12-13 0.18084706062668232 1107 14-15 0.3309988681713989 1107 16-17 0.33978761324673457 1107 18-19 0.30192036447817827 1107 20-21 0.16624549036514935 1107 22-23 0.22481142547175637 1107 24-25 0.13447457204916446 1107 26-27 0.18163321172402647 1107 28-29 0.5305048113322144 1107 30-31 0.7799434308377258 1107 32-33 0.5674903254775643 1107 34-35 1.0739911301468368 1107 36-37 0.790440107979336 1107 38-39 1.063599315595802 1107 40-41 1.4803631543234204 1107 42-43 0.8100514005945882 1107 44-45 1.1047574561164097 1107 46-47 1.6987891495306329 1107 48-49 1.4477829634214956 1107 50-51 1.843156414947618 1107 52-53 1.8732463816678582 1107 54-55 1.9086359407328182 1107 56-57 2.0959620300429656 1107 58-59 2.268813386023613 1107 60-61 2.177617265105024 1107 62-63 2.2934740753107263 1107 64-65 2.4007998182344608 1107 66-67 1.8592954343337809 1107 68-69 2.015612112254221 1107 70-71 1.8500410338371438 1107 72-73 2.2546678095802157 1107 74-75 2.383846252514978 1107 76-77 2.2333202550279054 1107 78-79 2.3958212782593655 1107 80-81 2.354228278444104 1107 82-83 1.7527543716244658 1107 84-85 1.9233334620386522 1107 86-87 2.0604796389585296 1107 88-89 2.497128783589517 1107 90-91 2.8568656622078947 1107 92-93 2.3146260299972354 1107 94-95 2.1669864088986266 1107 96-97 2.4994659152271126 1107 98-99 2.5396582571586457 1107 100-101 2.3800756871489384 1108 1 0.026190963896326025 1108 2 0.1921217567124316 1108 3 -0.2657086605720842 1108 4 -0.5742943256435993 1108 5 -0.14828063163928817 1108 6 -0.08595241203102688 1108 7 -0.18726898203050268 1108 8 -0.12960516753199158 1108 9 0.20470669658496377 1108 10-11 -0.03261972655797507 1108 12-13 -0.12846278840279268 1108 14-15 -0.20595745821250233 1108 16-17 -0.14819315598403904 1108 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-2.2624510616878943 2316 52-53 -2.9748752969101098 2316 54-55 -2.325796897823164 2316 56-57 -2.309104856524744 2316 58-59 -1.8574006509930676 2316 60-61 -1.890322995111216 2316 62-63 -2.2262986936099303 2316 64-65 -2.2831932182324657 2316 66-67 -2.53704871080091 2316 68-69 -2.57779085640988 2316 70-71 -2.0126490889409396 2316 72-73 -2.235885608048523 2316 74-75 -1.976223382815789 2316 76-77 -1.8339511395112211 2316 78-79 -1.7144774206594526 2316 80-81 -1.3790867298021858 2316 82-83 -1.1306156118829556 2316 84-85 -1.0720761036567836 2316 86-87 -0.7907206542428646 2316 88-89 -1.0110075550580895 2316 90-91 -1.6676533042571933 2316 92-93 -1.5214469038491956 2316 94-95 -1.5730722313799177 2316 96-97 -1.7136181969224182 2316 98-99 -2.4024802804994074 2316 100-101 -2.4442691909892353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4209.0 3 800.0 4 575.0 5 551.0 6 577.0 7 642.0 8 713.0 9 820.0 10 942.0 11 1278.0 12 1865.0 13 2395.0 14 2488.0 15 2968.0 16 3614.0 17 4232.0 18 3657.0 19 2203.0 20 2114.0 21 2182.0 22 2131.0 23 2388.0 24 2651.0 25 2912.0 26 3166.0 27 3667.0 28 4246.0 29 4697.0 30 5525.0 31 6786.0 32 7910.0 33 9794.0 34 12391.0 35 17058.0 36 23242.0 37 33609.0 38 32231.0 39 5623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.011726444047454 20.53914158765059 14.956254866932344 20.49287710136961 2 15.902163067656508 22.648133061157235 39.374147542634304 22.075556328551944 3 19.303041125243812 27.08854152358452 32.0893198904792 21.51909746069247 4 12.715448196322162 18.64456425230437 37.92300158353852 30.716985967834948 5 12.172913355532247 38.13968826862212 34.49564647847973 15.1917518973659 6 27.685447170105025 37.20480912745286 18.831323425724985 16.27842027671712 7 23.9980028765906 36.28260212354682 22.828586348104107 16.890808651758476 8 24.65666199415478 33.599483275155976 21.700611091260573 20.04324363942867 9 25.865461167491787 17.42681442572545 23.1352434638579 33.57248094292486 10-11 23.186003598917576 27.840557878012262 28.244405880979308 20.729032642090853 12-13 25.17354751263829 26.673199501795004 28.689830756832002 19.463422228734707 14-15 22.286887346893938 27.534603343158974 29.01874805470624 21.15976125524085 16-17 19.377974533297586 30.470448863805615 29.61231677333168 20.53925982956511 18-19 18.839554178488907 28.31910084570518 33.72904915990538 19.11229581590054 20-21 20.314109519427543 27.435252967810758 33.4271861995083 18.823451313253397 22-23 19.9842438177596 27.728819614614775 33.18691687239782 19.1000196952278 24-25 19.60078039486881 27.828358926682267 33.539118208755625 19.03174246969329 26-27 19.315399499307652 27.86178690313529 33.863217452704745 18.959596144852313 28-29 19.04868858921352 28.204957783665353 33.66924578982591 19.07710783729522 30-31 19.407376177732154 28.022693111412543 33.372209187227334 19.197721523627965 32-33 18.985035545728792 28.235622633908747 33.39032551922399 19.38901630113847 34-35 19.689462497735853 28.007300290956948 33.098932240735344 19.204304970571847 36-37 19.63046644722506 27.759287082382357 32.9423908430916 19.667855627300984 38-39 19.224488672589914 28.107526684433353 33.01874676048456 19.64923788249217 40-41 19.521337814725314 27.87098726654959 33.02099620793537 19.58667871078973 42-43 19.219223351289866 28.213772179069974 32.41509633003328 20.151908139606885 44-45 19.344562330567367 27.90850752498773 32.37946487956809 20.367465264876817 46-47 19.757835149913756 28.17878637748174 31.692676795478736 20.370701677125766 48-49 19.629875179797526 28.102095171676527 31.477815222054247 20.7902144264717 50-51 19.369134814114755 28.39810891840868 31.199405424128496 21.033350843348067 52-53 19.55498973494363 28.60636089414949 30.889217676541765 20.949431694365114 54-55 19.279855402231355 27.948840281855546 31.18508697886083 21.58621733705226 56-57 19.401680980610244 28.328536537822064 30.795656511219917 21.474125970347778 58-59 19.666207332983294 27.718227445837222 31.01029717590034 21.605268045279143 60-61 19.431915069171318 27.870220119731933 31.195164883522647 21.5026999275741 62-63 19.427727422846868 27.609537264687532 31.28509045539613 21.677644857069463 64-65 19.337846797635134 27.970019340141 30.84993381221021 21.84220005001365 66-67 19.259563474673687 27.835659434072653 30.494825066364424 22.40995202488924 68-69 19.276809868316892 27.60802076091221 30.481920246707922 22.63324912406297 70-71 19.274902427775903 27.487259970034255 30.476492034756593 22.761345567433246 72-73 19.392191547305217 27.06275624568383 30.46888801705131 23.076164189959645 74-75 19.004739771408122 27.527381012466563 30.217075106793978 23.250804109331337 76-77 19.04586044825933 27.23435783215163 30.250279273224407 23.469502446364633 78-79 19.091638765307525 26.906159702793193 30.302022657432463 23.700178874466815 80-81 19.255820780507495 27.00906372126305 30.010274663779974 23.72484083444948 82-83 19.095884252633123 26.87965613417006 29.90660207714044 24.11785753605637 84-85 18.992405423321383 26.438347680683716 30.1656769173955 24.403569978599396 86-87 19.215146615291108 26.272175777800715 30.131305833648696 24.38137177325948 88-89 19.469395994091148 25.83838736019231 30.16313572929378 24.529080916422757 90-91 19.369899533573314 25.51672872688228 30.55656886160228 24.556802877942125 92-93 19.66187864063192 25.479089380309674 30.416698212118497 24.442333766939907 94-95 19.468248887787574 24.96495297618638 30.449267282025865 25.11753085400018 96-97 19.743665048320928 24.62187611854 30.44172578676383 25.192733046375242 98-99 19.69227662981879 24.65136768491623 30.27651968814673 25.37983599711825 100-101 20.16639026706395 23.864639889164906 30.94712165940688 25.02184818436427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 892.0 1 594.0 2 316.5 3 327.5 4 273.0 5 264.5 6 392.5 7 576.0 8 753.0 9 838.0 10 744.5 11 646.0 12 630.5 13 663.0 14 698.5 15 699.5 16 752.0 17 780.5 18 840.0 19 948.5 20 1025.0 21 1150.5 22 1296.0 23 1432.5 24 1594.5 25 1810.5 26 2171.5 27 2633.0 28 2968.0 29 3208.5 30 3528.0 31 3989.5 32 4413.0 33 4822.5 34 5306.0 35 5814.5 36 6260.0 37 6671.5 38 6770.0 39 6478.5 40 6332.0 41 6389.5 42 6537.0 43 6706.5 44 6835.5 45 6919.5 46 7085.0 47 7406.0 48 8069.0 49 8232.5 50 7324.5 51 6367.0 52 5822.0 53 5494.5 54 5148.0 55 4704.0 56 4172.5 57 3666.5 58 3408.0 59 3063.5 60 2573.5 61 2222.0 62 1877.5 63 1437.0 64 1064.5 65 848.0 66 707.0 67 588.0 68 455.5 69 342.0 70 292.0 71 260.5 72 203.5 73 168.5 74 145.0 75 119.5 76 91.5 77 62.5 78 41.0 79 34.0 80 29.0 81 20.0 82 13.5 83 8.5 84 8.5 85 7.5 86 4.0 87 3.0 88 2.5 89 3.5 90 3.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24765595013982053 2 0.16723630581397475 3 0.2037906895984501 4 0.16175314824630344 5 0.23897428399100762 6 0.1983075320307788 7 0.24582823095059672 8 0.2531391077074918 9 0.1361650795971707 10-11 0.20698919817959172 12-13 0.21384314513918082 14-15 0.17180560378703416 16-17 0.2949481841609855 18-19 0.23463345091660118 20-21 0.1941951638550253 22-23 0.23988814358561952 24-25 0.2289218284502769 26-27 0.3445250671686802 28-29 0.3152815601410999 30-31 0.29060735108657904 32-33 0.1832288487196827 34-35 0.35571984720267574 36-37 0.3995851077440462 38-39 0.38427795953429716 40-41 0.3495512949390456 42-43 0.377195547676055 44-45 0.3861056787235209 46-47 0.39295962568311005 48-49 0.4105514228793888 50-51 0.40278361632518783 52-53 0.40186975673057596 54-55 0.3966150640615576 56-57 0.3767386178787491 58-59 0.35754756639189955 60-61 0.3189369985195475 62-63 0.385648748926215 64-65 0.41649151024436604 66-67 0.4233454572039552 68-69 0.43065633396085023 70-71 0.42791475517701455 72-73 0.4210608082174255 74-75 0.41534918575110125 76-77 0.40004203754135215 78-79 0.3755962933854843 80-81 0.3838210297369912 82-83 0.39295962568311005 84-85 0.3963865991629046 86-87 0.38816186281139764 88-89 0.39935664284539324 90-91 0.41969001882550766 92-93 0.416948440041672 94-95 0.4251731763931789 96-97 0.42677243068374976 98-99 0.42448778169722 100-101 0.3977573885548224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 218852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.92664323893219 #Duplication Level Percentage of deduplicated Percentage of total 1 90.2086926700515 68.49243225409478 2 6.304960463124136 9.574289674383978 3 1.404859758566136 3.1999885706815028 4 0.6286808432685106 1.90934504391997 5 0.3403741291107409 1.2921732534376733 6 0.2478995135695231 1.1293306755538808 7 0.15523184439814403 0.8250363008257522 8 0.12122820546181881 0.736356056527639 9 0.09764499014632584 0.6672470697818217 >10 0.44765251645961357 5.940832738210615 >50 0.028315888657032936 1.4386236239778083 >100 0.011446848606034591 1.7693469562944875 >500 0.0012049314322141672 0.7245722799303175 >1k 0.001807397148321251 2.3004255023797766 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2070 0.9458446804232998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1842 0.841664686637545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1117 0.5103905835907371 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 956 0.4368248862244805 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.28695191270813153 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 449 0.2051614789903679 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 427 0.19510902344963718 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 373 0.17043481439511635 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 317 0.14484674574598358 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 291 0.1329665710160291 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0013707893919178257 0.0 22-23 0.0 0.0 0.0 0.00959552574342478 0.0 24-25 0.0 0.0 0.0 0.017363332297625794 0.0 26-27 0.0 0.0 0.0 0.02627346334509166 0.0 28-29 0.0 0.0 0.0 0.06716868020397346 0.0 30-31 0.0 0.0 0.0 0.1535284118947965 0.0 32-33 0.0 0.0 0.0 0.3027159907151865 0.0 34-35 0.0 0.0 0.0 0.5638513698755323 0.0 36-37 0.0 0.0 0.0 0.905177928463071 0.0 38-39 0.0 0.0 0.0 1.3433736040794693 0.0 40-41 0.0 0.0 0.0 1.7747153327362784 0.0 42-43 0.0 0.0 0.0 2.187551404602197 0.0 44-45 0.0 0.0 0.0 2.691544971030651 0.0 46-47 0.0 0.0 0.0 3.288066821413558 0.0 48-49 0.0 0.0 0.0 3.8578582786540676 0.0 50-51 0.0 0.0 0.0 4.4207957889349885 0.0 52-53 0.0 0.0 0.0 4.983961764114562 0.0 54-55 0.0 0.0 0.0 5.622521155849615 0.0 56-57 0.0 0.0 0.0 6.3785115054922965 0.0 58-59 0.0 0.0 0.0 7.0997751905397255 0.0 60-61 0.0 0.0 0.0 7.90442856359549 0.0 62-63 0.0 0.0 0.0 8.685093122292692 0.0 64-65 0.0 0.0 0.0 9.452735181766673 0.0 66-67 0.0 0.0 0.0 10.37070714455431 0.0 68-69 0.0 0.0 0.0 11.32112112295067 0.0 70-71 0.0 0.0 0.0 12.34395847422002 0.0 72-73 0.0 0.0 0.0 13.386215341874873 0.0 74-75 0.0 0.0 0.0 14.407681903752309 0.0 76-77 0.0 0.0 0.0 15.468215963299398 0.0 78-79 0.0 0.0 0.0 16.62881764845649 0.0 80-81 2.2846489865297097E-4 0.0 0.0 17.788733938917623 0.0 82-83 4.5692979730594193E-4 0.0 0.0 18.971496719244055 0.0 84-85 4.5692979730594193E-4 0.0 0.0 20.19195620784823 0.0 86-87 4.5692979730594193E-4 0.0 0.0 21.39710854824265 0.0 88-89 4.5692979730594193E-4 0.0 0.0 22.65754939411109 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACC 40 5.4234546E-4 47.41993 4 TAATACC 55 0.00259646 34.487225 4 AGCTCCG 60 0.003976709 31.613289 5 GGTATCA 2130 0.0 30.77212 1 GTCGGGA 65 0.00588213 29.181498 2 GTTATTC 85 6.4537826E-4 27.89408 3 CGACGAG 35 0.008365861 27.156206 90-91 AAGCCGA 35 0.008422237 27.118849 24-25 GGGGCGC 70 0.008446162 27.097103 6 ATGGGGA 180 6.7557266E-9 26.344408 5 GTACATG 1340 0.0 25.87474 1 TACATGG 1385 0.0 24.651516 2 ACATGGG 1360 0.0 24.40732 3 GTATCAA 2745 0.0 24.012278 2 CATGGGG 480 0.0 23.709967 4 CGATACC 55 0.0028644295 21.604008 94-95 ATCAACG 3245 0.0 21.189184 4 AACGCTT 90 5.1397965E-6 21.11907 42-43 GAGTACT 1775 0.0 21.114883 12-13 TATCAAC 3230 0.0 20.993963 3 >>END_MODULE