FastQCFastQC Report
Thu 26 May 2016
SRR938820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218852
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT20400.9321367865041215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.888728455760057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.8357245992725677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.5222707583206916No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT11000.5026227770365361RNA PCR Primer, Index 46 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9880.4514466397382706No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.26136384405899876No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT5570.25450989709940963RNA PCR Primer, Index 46 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4910.22435253047721745No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG4540.20744612797689763RNA PCR Primer, Index 46 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.1928243744631075No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.16221007804360937No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2630.12017253669146272No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.117887887704933No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT2390.10920622155612013RNA PCR Primer, Index 46 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG156.6327496E-494.9204565
GGCTTAG405.334172E-447.5798571
CGGGTCG550.002591484534.500766
GGTATCA17650.033.696781
GCTTAGA600.003960227631.6401522
GCCTTAG650.00578614729.2799131
TTATCCG650.005870899729.1929479
TAGTAAG650.005870899729.1929477
GTCTTAA1056.802412E-527.1884921
CCGGGTC700.0084112827.120135
GTCGGGA700.0084300627.107749
GTATCAA23850.025.3360251
TAGGGCA950.001229369724.9790694
TGTGGAC1151.2844062E-424.7618583
TAGGAGG1151.2844062E-424.7618584
GTTATAG1000.00163332223.7899281
ATGGGAT1801.9604158E-723.719277
GTAGACC1050.00220127122.600113
CTAGGAG1503.0383395E-522.1481063
CCTATAG1100.002842142521.6272091
Produced by FastQC (version 0.11.5)