##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938820_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 218852 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.891072505620237 31.0 31.0 34.0 30.0 34.0 2 32.08579770804014 33.0 31.0 34.0 30.0 34.0 3 32.13089210973626 34.0 31.0 34.0 30.0 34.0 4 35.81481549174785 37.0 35.0 37.0 35.0 37.0 5 35.72125454645148 37.0 35.0 37.0 33.0 37.0 6 35.76263867819348 37.0 35.0 37.0 33.0 37.0 7 35.673925758046536 37.0 35.0 37.0 33.0 37.0 8 35.67831685339864 37.0 35.0 37.0 33.0 37.0 9 37.261057701094806 39.0 37.0 39.0 33.0 39.0 10-11 37.212511194780035 39.0 37.0 39.0 33.0 39.0 12-13 37.11849103503738 39.0 37.0 39.0 33.0 39.0 14-15 38.24888280664558 40.0 37.5 41.0 33.0 41.0 16-17 38.177238499077006 40.0 37.0 41.0 33.0 41.0 18-19 38.32274322373111 40.0 38.0 41.0 33.0 41.0 20-21 38.35458894595435 40.0 38.0 41.0 33.5 41.0 22-23 38.23703004770347 40.0 37.5 41.0 33.0 41.0 24-25 38.11001727194633 40.0 37.0 41.0 33.0 41.0 26-27 37.85575868623545 40.0 37.0 41.0 33.0 41.0 28-29 37.59924286732586 40.0 36.5 41.0 32.0 41.0 30-31 37.2342770456747 39.0 36.0 41.0 32.0 41.0 32-33 36.817943176210406 39.0 35.0 41.0 31.0 41.0 34-35 36.575902893279476 39.0 35.0 41.0 30.5 41.0 36-37 36.26441841975399 39.0 35.0 41.0 30.0 41.0 38-39 35.92625610001279 39.0 35.0 41.0 29.5 41.0 40-41 35.6482188876501 39.0 35.0 41.0 28.5 41.0 42-43 35.26183448175023 39.0 35.0 41.0 26.5 41.0 44-45 34.77646765850895 38.5 35.0 41.0 24.0 41.0 46-47 34.161725732458464 38.0 34.0 40.5 20.0 41.0 48-49 33.62275190539725 38.0 33.5 40.0 10.5 41.0 50-51 33.2027488896606 38.0 33.0 40.0 4.0 41.0 52-53 32.95060360426224 38.0 33.0 40.0 2.0 41.0 54-55 32.61772567762689 38.0 33.0 40.0 2.0 41.0 56-57 32.223317584486324 37.0 32.0 40.0 2.0 41.0 58-59 31.83141803593296 37.0 31.5 40.0 2.0 41.0 60-61 31.357159176064187 36.5 31.0 40.0 2.0 41.0 62-63 30.93937912379142 36.0 30.5 39.5 2.0 41.0 64-65 30.45865470729077 35.5 30.0 39.0 2.0 40.5 66-67 29.95139409281158 35.0 29.0 39.0 2.0 40.0 68-69 29.554932100232122 35.0 29.0 38.0 2.0 40.0 70-71 29.118374517939063 35.0 28.0 37.5 2.0 40.0 72-73 28.652498035201873 34.5 27.5 37.0 2.0 39.5 74-75 28.16974028110321 34.0 26.0 36.5 2.0 39.0 76-77 26.35083298302049 32.0 24.0 34.5 2.0 36.5 78-79 27.175518158390144 33.5 25.5 35.0 2.0 37.5 80-81 27.151319156324824 34.0 25.5 35.0 2.0 37.0 82-83 26.807833604445015 34.0 25.0 35.0 2.0 37.0 84-85 26.398335404748416 34.0 23.5 35.0 2.0 36.0 86-87 25.816104033776252 33.0 21.0 35.0 2.0 36.0 88-89 25.222045035000825 33.0 17.0 35.0 2.0 35.0 90-91 24.70073154460549 32.0 9.5 35.0 2.0 35.0 92-93 24.373144865022937 32.0 4.5 35.0 2.0 35.0 94-95 24.003433827426754 32.0 2.0 34.5 2.0 35.0 96-97 23.398015553890303 31.5 2.0 34.0 2.0 35.0 98-99 22.64552528649498 31.0 2.0 34.0 2.0 35.0 100-101 19.72590380713907 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1401580595834524 1101 2 0.06957014665194095 1101 3 0.25372112138755654 1101 4 0.18737776213749413 1101 5 -0.328709293613187 1101 6 0.011936790437154343 1101 7 0.2534489147078176 1101 8 0.2395878591694256 1101 9 0.26694208997471947 1101 10-11 0.44031427435115233 1101 12-13 0.5740439500417907 1101 14-15 0.5248543708378506 1101 16-17 0.6484927845480186 1101 18-19 0.7622018952343481 1101 20-21 0.8003929146011828 1101 22-23 0.6308000394710191 1101 24-25 0.37353729886828546 1101 26-27 0.48014178357512094 1101 28-29 0.5398222956653314 1101 30-31 0.5661914771034091 1101 32-33 0.3428361769160233 1101 34-35 0.7006438190689153 1101 36-37 0.4703597172632925 1101 38-39 1.044669130146275 1101 40-41 0.8623655126346605 1101 42-43 0.22503006236515688 1101 44-45 -0.55751503793784 1101 46-47 -0.6536145871384207 1101 48-49 -0.754999593862145 1101 50-51 -0.5439339247359314 1101 52-53 -0.5055041575641823 1101 54-55 -1.0647499494747201 1101 56-57 -0.7486842627417829 1101 58-59 -0.32575747830802726 1101 60-61 -0.16147972582915315 1101 62-63 -0.14464108518933472 1101 64-65 -0.34586950073826017 1101 66-67 -0.308869877144069 1101 68-69 -0.017600695922741494 1101 70-71 0.16060468111570358 1101 72-73 0.24733068157145155 1101 74-75 0.08661594923608007 1101 76-77 -0.27240964615214125 1101 78-79 0.13532546126399936 1101 80-81 0.3609441205009425 1101 82-83 0.464893611973455 1101 84-85 0.4413190432441816 1101 86-87 0.3082538208100871 1101 88-89 0.3444096902907141 1101 90-91 -0.05394448306742561 1101 92-93 -0.1528518810377939 1101 94-95 0.137271146043755 1101 96-97 0.296758177471407 1101 98-99 0.004787818226891005 1101 100-101 0.4681397063434751 1102 1 0.15113709959797106 1102 2 0.07628460087569522 1102 3 -0.08522271311021967 1102 4 -0.04490605965725791 1102 5 0.06032073995908149 1102 6 0.057713035786939315 1102 7 0.15903824416975 1102 8 0.02885380599821019 1102 9 0.09436246925064751 1102 10-11 0.07306084907214228 1102 12-13 -0.11178310739401098 1102 14-15 0.3344905200076198 1102 16-17 0.3127700733602907 1102 18-19 0.14097877573521345 1102 20-21 0.088932974486859 1102 22-23 -0.1266176177288756 1102 24-25 0.17972548505829167 1102 26-27 -0.09371721288618318 1102 28-29 -0.38559282093026326 1102 30-31 -0.6976230587826251 1102 32-33 -0.8271801555401979 1102 34-35 -0.5102490218711111 1102 36-37 -0.4381331599710805 1102 38-39 -0.7440750854421481 1102 40-41 -0.7108810167183961 1102 42-43 -0.5598351948710274 1102 44-45 0.04227173277262608 1102 46-47 0.4466258629114819 1102 48-49 0.30572538572229035 1102 50-51 0.3120228849909594 1102 52-53 0.24052614821568596 1102 54-55 -0.2588112869214143 1102 56-57 -0.43387616910236915 1102 58-59 -0.30112269017627824 1102 60-61 -0.4048333235063133 1102 62-63 -0.030359259583676135 1102 64-65 0.26485094205277804 1102 66-67 -0.13674393575944066 1102 68-69 0.3404884066995244 1102 70-71 -0.014470175975304755 1102 72-73 0.27205620557109356 1102 74-75 0.34779914495334907 1102 76-77 0.23466775154317077 1102 78-79 0.006548580763137579 1102 80-81 -0.0016817755157525482 1102 82-83 -0.44555008642051774 1102 84-85 -0.41291378499282416 1102 86-87 -0.29559337998838586 1102 88-89 -0.3281472464663828 1102 90-91 -0.26234208764051203 1102 92-93 -0.06089107893271972 1102 94-95 0.17229519104874313 1102 96-97 0.13493075709213187 1102 98-99 0.3201176431066628 1102 100-101 0.7878929047828187 1103 1 0.14950385397597543 1103 2 0.022841174689325783 1103 3 0.07147813073335385 1103 4 0.22943383690385133 1103 5 0.2787673419008314 1103 6 0.19417978109135703 1103 7 0.04316854087603872 1103 8 -0.026767281017491484 1103 9 -0.07885230254863984 1103 10-11 0.11788436780908995 1103 12-13 0.10675423041562482 1103 14-15 -0.04523908710606861 1103 16-17 -0.029077308909926103 1103 18-19 -0.018171936541349965 1103 20-21 0.2139443164703394 1103 22-23 0.39481873105980014 1103 24-25 0.3571821586813684 1103 26-27 0.0829455218928743 1103 28-29 0.06785967884290045 1103 30-31 0.12226624345855441 1103 32-33 0.1465744946730254 1103 34-35 0.18896157607826325 1103 36-37 0.32316345558105297 1103 38-39 -0.4763589072369001 1103 40-41 -0.6446438331597335 1103 42-43 -0.4595493768871748 1103 44-45 -0.25377672018082365 1103 46-47 -0.6302501011571167 1103 48-49 -0.8437846405911174 1103 50-51 -1.0602890649228414 1103 52-53 -1.1269994846669888 1103 54-55 -0.9572733139607017 1103 56-57 -0.9332637019941217 1103 58-59 -1.1575331792426056 1103 60-61 -0.6848442118104607 1103 62-63 -1.0301551038809258 1103 64-65 -0.45801903344853656 1103 66-67 -0.6172810920973397 1103 68-69 -1.120404434240502 1103 70-71 -0.8978065338375671 1103 72-73 -0.6194917483350899 1103 74-75 -0.953103676932141 1103 76-77 -0.9102601134418578 1103 78-79 -0.7805623892032898 1103 80-81 -0.06896242155513121 1103 82-83 -0.425293303914394 1103 84-85 -0.5329800221763819 1103 86-87 -0.35062468386281154 1103 88-89 -0.6999828330737721 1103 90-91 -0.8343183148431237 1103 92-93 -0.4869640305705012 1103 94-95 -0.5029157698440976 1103 96-97 0.03974883167701648 1103 98-99 -0.41110003224039815 1103 100-101 1.0545883044743185 1104 1 0.15092604007918098 1104 2 0.0560932402453318 1104 3 0.04976157468116327 1104 4 0.08656056948771607 1104 5 -0.1318606774228428 1104 6 -0.11064210703000299 1104 7 0.1501033912078853 1104 8 0.09292209547806607 1104 9 0.11666442506885488 1104 10-11 0.2889981878461114 1104 12-13 0.2842114519606156 1104 14-15 0.44000855387093907 1104 16-17 0.22330428845338446 1104 18-19 0.3559194445593832 1104 20-21 0.002128884622401017 1104 22-23 0.20858007468705608 1104 24-25 0.06709005520040279 1104 26-27 0.11027181201884417 1104 28-29 0.6953485045235155 1104 30-31 0.4662998341398463 1104 32-33 0.05680746230136435 1104 34-35 0.32527698535181315 1104 36-37 0.8598242529082398 1104 38-39 1.1123809907268907 1104 40-41 1.3133791024129735 1104 42-43 1.1973877308829302 1104 44-45 1.1105963892146278 1104 46-47 1.4605440656224289 1104 48-49 1.219073727732514 1104 50-51 0.6959753262460637 1104 52-53 1.2032975942079034 1104 54-55 1.4833560598259226 1104 56-57 1.1878817221785312 1104 58-59 1.312239722468533 1104 60-61 1.6531845931017273 1104 62-63 1.9216598764793602 1104 64-65 1.935023541837026 1104 66-67 2.1127518665012452 1104 68-69 1.995300563727806 1104 70-71 1.9990962608709957 1104 72-73 2.0061798395469843 1104 74-75 1.5090955194198372 1104 76-77 1.6612893921791638 1104 78-79 1.6454726462384919 1104 80-81 1.8718701216748137 1104 82-83 2.069446864941991 1104 84-85 2.190833694018483 1104 86-87 2.191860304172767 1104 88-89 2.4861203770034344 1104 90-91 2.4623542211630145 1104 92-93 2.065510121761431 1104 94-95 1.6303295233971333 1104 96-97 1.946302174943078 1104 98-99 2.0423515908577095 1104 100-101 2.775557648462758 1105 1 -0.13709370550654043 1105 2 -0.36970259546126627 1105 3 -0.11290745470516583 1105 4 -0.18065880481348984 1105 5 -0.2539837498022237 1105 6 -0.16734737884824114 1105 7 -0.0398212971029821 1105 8 -0.14132468215204597 1105 9 -0.20179643183893603 1105 10-11 -0.24756067973610385 1105 12-13 -0.009904954174018599 1105 14-15 0.10030431574255516 1105 16-17 -0.12367797005345693 1105 18-19 -0.06280932788119031 1105 20-21 -0.1327887366251943 1105 22-23 -0.017987864051001168 1105 24-25 -0.10742421604825125 1105 26-27 -0.05576198331056759 1105 28-29 0.20332268337296 1105 30-31 0.04009263206403091 1105 32-33 -0.40942885185596367 1105 34-35 -0.16553583240233394 1105 36-37 0.145164843166242 1105 38-39 0.13403573831671167 1105 40-41 -0.2217874482272748 1105 42-43 -0.3561742799602712 1105 44-45 -0.4171444710684753 1105 46-47 -0.28802669994092867 1105 48-49 -0.39535995789919554 1105 50-51 -0.49803138219011345 1105 52-53 -0.9024657541033818 1105 54-55 -0.780825532056447 1105 56-57 -0.5553201848737643 1105 58-59 -0.6276796919145227 1105 60-61 -0.7968713105330636 1105 62-63 -0.26152473208971827 1105 64-65 -0.42645147029300645 1105 66-67 -0.2356543525144339 1105 68-69 -0.6548798046033149 1105 70-71 -0.6516325959524103 1105 72-73 -0.5678450112008591 1105 74-75 -0.543327323016296 1105 76-77 -0.20792774597664732 1105 78-79 -0.6029820929130381 1105 80-81 -0.3904128561957876 1105 82-83 -0.38048246442535216 1105 84-85 -0.7625131405700429 1105 86-87 -0.4690999726029652 1105 88-89 -0.7769325943274943 1105 90-91 -0.5536996150920785 1105 92-93 -1.236453888955193 1105 94-95 -0.5801001962408598 1105 96-97 -0.31293247265306334 1105 98-99 0.24175880137180883 1105 100-101 0.8589489950020059 1106 1 0.1815218532836944 1106 2 0.04945072816803986 1106 3 0.106524859705317 1106 4 0.15942691409318854 1106 5 0.038026601160090934 1106 6 0.07856884305051892 1106 7 -0.10251335597408229 1106 8 -0.03308434574577035 1106 9 -0.029873417121159207 1106 10-11 0.001256641952402049 1106 12-13 0.023442530865608546 1106 14-15 -0.04549869250587335 1106 16-17 0.08733407911361013 1106 18-19 -0.10472870358876918 1106 20-21 -0.016087701528967102 1106 22-23 -0.06476936170586356 1106 24-25 -0.06173442145016139 1106 26-27 -0.12261868717600777 1106 28-29 -0.10759030385007406 1106 30-31 -0.39752866827561206 1106 32-33 -0.47368770678075833 1106 34-35 -0.09803219339215019 1106 36-37 -0.37437461987758525 1106 38-39 -0.17757472585925882 1106 40-41 -0.3510733935612578 1106 42-43 -0.1804303045438047 1106 44-45 -0.25433919854704357 1106 46-47 0.04760123148393802 1106 48-49 0.3711469966536072 1106 50-51 0.06332637294493537 1106 52-53 0.06199324638181025 1106 54-55 0.09205171199983653 1106 56-57 0.4135258445617822 1106 58-59 0.6417485791512973 1106 60-61 0.516912451394802 1106 62-63 0.10936116472412749 1106 64-65 0.10169020850369392 1106 66-67 0.2820352803498736 1106 68-69 0.287067874516854 1106 70-71 0.5576711400980479 1106 72-73 0.5481484385807995 1106 74-75 0.4977227335905319 1106 76-77 0.26293995578624774 1106 78-79 0.45827457860563925 1106 80-81 0.7078202021901099 1106 82-83 0.7166892159886906 1106 84-85 0.4655921481247631 1106 86-87 1.0633377598893539 1106 88-89 0.8108989599341889 1106 90-91 0.4488679087636598 1106 92-93 0.7476759832751192 1106 94-95 1.075679764943029 1106 96-97 0.8527031411266499 1106 98-99 1.3456929757208336 1106 100-101 1.053939290974835 1107 1 -0.018720445089446258 1107 2 -0.173420507553665 1107 3 -0.13880224309841793 1107 4 0.164013276156183 1107 5 -0.01562518146365477 1107 6 0.11006763155864974 1107 7 -0.012906225479099476 1107 8 -0.162281299709079 1107 9 -0.07885230254863984 1107 10-11 -0.22791002471426935 1107 12-13 -0.05212427425727384 1107 14-15 -0.4120615170126172 1107 16-17 0.02466100884708311 1107 18-19 -0.22611586177499277 1107 20-21 -0.23465381437078037 1107 22-23 0.002295366573818569 1107 24-25 -0.1474907385148967 1107 26-27 -0.26051242203235603 1107 28-29 0.17767276295504786 1107 30-31 0.2203970845800427 1107 32-33 -0.012304009999866139 1107 34-35 0.28475596860162256 1107 36-37 -0.3497337406806338 1107 38-39 -0.2286953558350291 1107 40-41 -0.4250176649354316 1107 42-43 -0.008614797447926037 1107 44-45 0.6551017844920679 1107 46-47 0.6594695250111116 1107 48-49 0.6305144248294425 1107 50-51 0.2084025238622047 1107 52-53 -0.08027051270437369 1107 54-55 0.4118855645439652 1107 56-57 0.08075498959466643 1107 58-59 -0.06874813251363321 1107 60-61 0.6329127975353401 1107 62-63 0.11937760639944628 1107 64-65 0.08637348991594962 1107 66-67 0.21683105743536757 1107 68-69 0.3375394909931444 1107 70-71 0.50639907363907 1107 72-73 0.28938675633780875 1107 74-75 0.827270154843557 1107 76-77 0.3631043725394534 1107 78-79 0.3993441528527839 1107 80-81 0.8095422513420658 1107 82-83 0.6284450138426187 1107 84-85 0.40860876287035097 1107 86-87 0.21012297968859173 1107 88-89 0.021979783748658832 1107 90-91 0.3759620589886481 1107 92-93 0.8681761563453883 1107 94-95 1.0040935759502965 1107 96-97 0.80610397186393 1107 98-99 1.0211429584138045 1107 100-101 1.2742144726986133 1108 1 0.21321700997885173 1108 2 0.1610960273133415 1108 3 0.231524859705317 1108 4 0.27926309642936786 1108 5 0.12011349574698471 1108 6 0.20339078037245173 1108 7 -0.1279763189370442 1108 8 -0.14170257936400077 1108 9 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-2.311769389675799 2316 84-85 -2.1646366100451715 2316 86-87 -1.9632689939232826 2316 88-89 -2.337494286253172 2316 90-91 -2.42667675354571 2316 92-93 -2.2056464568120298 2316 94-95 -2.4930293853837675 2316 96-97 -2.520989669330863 2316 98-99 -2.5362787018390804 2316 100-101 -1.9204071142539227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 19.0 6 52.0 7 106.0 8 263.0 9 493.0 10 822.0 11 1304.0 12 1786.0 13 2236.0 14 2542.0 15 3108.0 16 3604.0 17 4603.0 18 4179.0 19 2720.0 20 2446.0 21 2546.0 22 2659.0 23 2636.0 24 2864.0 25 2995.0 26 2992.0 27 3545.0 28 4038.0 29 4815.0 30 5739.0 31 6683.0 32 7999.0 33 10033.0 34 12919.0 35 17804.0 36 25047.0 37 37731.0 38 32415.0 39 3104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.20663995420721 21.933371493989696 15.651516886090441 21.20847166571265 2 15.687313801107598 23.35276808071208 39.216913713377075 21.743004404803244 3 18.49194889697147 27.840275620053735 33.19320819549284 20.47456728748195 4 12.422093469559336 20.350739312412042 36.32317730703855 30.903989910990077 5 11.561824406163016 39.21495139152334 34.60320217195563 14.620022030358015 6 27.005538191588528 38.29120286596846 17.995010144212316 16.708248798230706 7 23.0776963427339 36.64028658636887 21.829821066291373 18.452196004605852 8 24.36258293275821 34.88019300714638 21.451483194122055 19.305740865973352 9 24.4000511761373 17.504980534790633 22.283552355016177 35.81141593405589 10-11 22.834381225668487 28.30954252188694 27.98832087438086 20.867755378063716 12-13 24.846471588105203 26.686299416957578 28.321422696616892 20.145806298320327 14-15 21.88396724727213 27.890309432858736 27.929833860325697 22.295889459543435 16-17 19.381150284566683 31.273775769221103 28.637849767982765 20.70722417822945 18-19 19.02724215451538 28.66412004459635 32.4744576243306 19.834180176557673 20-21 20.010326613419114 27.713477601301335 32.466004423080435 19.810191362199113 22-23 19.829382413685963 28.711412278617516 32.09863286604646 19.360572441650064 24-25 19.844323306724668 28.97108078098798 32.61511256516991 18.569483347117448 26-27 19.507191080546956 28.912370486297533 33.190235209449504 18.390203223706006 28-29 18.613035293257543 28.72763328642187 33.424186208030996 19.235145212289584 30-31 19.309167839453146 29.41120026319156 32.373476139125984 18.906155758229307 32-33 20.063330469906603 28.57341947983112 32.66545427960448 18.697795770657795 34-35 19.4701042563935 28.41974908093065 32.768906324613695 19.341240338062153 36-37 19.04773874514123 28.865362159543523 32.65388667103889 19.433012424276356 38-39 18.970698121873696 27.943612793835705 33.083266874915736 20.002422209374863 40-41 18.991127755296958 27.877310313784537 33.3062192538906 19.8253426770279 42-43 19.42972564772226 27.92900246544605 32.80437062577545 19.836901261056234 44-45 18.88493112500771 28.260109624220604 32.425053178485506 20.429906072286183 46-47 19.94695008693149 27.797743208263174 31.394719226138392 20.860587478666943 48-49 19.73227871526683 27.743354946606537 31.199422427540703 21.324943910585933 50-51 18.976979876811725 28.137736918099904 31.55168789867125 21.333595306417124 52-53 19.123654341746935 28.498254528174293 31.649927805092027 20.72816332498675 54-55 18.86823058505291 28.172006652897846 30.99903130882971 21.96073145321953 56-57 19.56901692266568 28.86288916762052 29.84598934879609 21.722104560917714 58-59 18.929002248094605 29.118765192915763 30.734697421088224 21.217535137901415 60-61 19.140380458871967 29.985240425697196 29.766588527743888 21.10779058768695 62-63 18.46798477489045 30.22312187855553 29.69147677165534 21.617416574898673 64-65 18.348831364664488 30.395942333615118 29.459891704174186 21.79533459754621 66-67 18.604544893734666 30.063197144933035 28.91029478292261 22.421963178409698 68-69 18.67032213845099 29.49851496458762 29.034498514964586 22.796664381996802 70-71 18.710361340083708 29.21654816953923 29.016641383217884 23.056449107159178 72-73 18.814312868970813 28.68513881527242 28.920686125783636 23.579862189973134 74-75 18.71944254055289 29.14347726753484 28.36394790952707 23.773132282385195 76-77 18.764801623949598 28.76612748370107 28.653886597843876 23.815184294505453 78-79 18.664726713701377 28.71633678315535 28.568552630374906 24.050383872768368 80-81 18.70681934493347 28.72153275332651 28.243118511478286 24.328529390261735 82-83 18.60213800871379 28.470151725965682 28.403667377203867 24.524042888116664 84-85 18.83578856944419 28.156242574890793 28.259280244183284 24.748688611481732 86-87 18.648250561494514 28.424887815454706 28.004510253278625 24.922351369772155 88-89 18.969706973310778 27.949867723048282 28.111488608593188 24.968936695047752 90-91 18.86221708509062 27.84050123726897 28.063874727376554 25.233406950263852 92-93 18.91922148123271 27.846507791813167 27.923272173140234 25.310998553813885 94-95 18.940240299929403 27.43869701601314 28.11061381804557 25.510448866011888 96-97 18.949792553872022 27.310008590280187 27.957478113062713 25.782720742785077 98-99 18.78118545866613 27.127236671357814 27.910642808838848 26.180935061137205 100-101 18.79797850562948 26.700495320953355 28.664187015645563 25.837339157771606 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 655.0 1 441.0 2 244.0 3 273.0 4 244.0 5 215.0 6 307.0 7 493.0 8 696.5 9 774.0 10 657.5 11 543.5 12 534.0 13 571.5 14 612.0 15 667.0 16 708.0 17 728.5 18 789.5 19 856.5 20 944.0 21 1083.0 22 1170.5 23 1309.0 24 1536.5 25 1751.0 26 2109.5 27 2553.5 28 2849.5 29 3141.5 30 3601.5 31 4079.5 32 4513.5 33 4931.0 34 5459.0 35 5889.0 36 6202.0 37 6676.5 38 6725.5 39 6473.0 40 6543.0 41 6765.5 42 6937.5 43 7168.5 44 7500.0 45 7669.5 46 7670.5 47 7873.0 48 8414.5 49 8507.0 50 7851.0 51 6930.5 52 6002.5 53 5373.0 54 4926.5 55 4499.0 56 3939.0 57 3433.5 58 3168.0 59 2780.0 60 2397.0 61 2083.5 62 1692.0 63 1246.0 64 890.0 65 680.5 66 505.0 67 401.5 68 320.0 69 223.5 70 179.0 71 133.5 72 108.5 73 98.0 74 79.0 75 60.5 76 39.0 77 26.0 78 14.5 79 9.0 80 7.0 81 5.5 82 4.5 83 4.5 84 3.0 85 2.0 86 1.0 87 0.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2179555133149343 2 0.0 3 0.0 4 0.0 5 0.02878657723027434 6 0.0036554383784475355 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.00479776287171239 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.5692979730594193E-4 26-27 0.0020561840878767384 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007539341655548042 36-37 0.021247235574726298 38-39 0.019876446182808472 40-41 0.010737850236689635 42-43 0.012565569425913404 44-45 0.006168552263630216 46-47 6.853946959589129E-4 48-49 0.0022846489865297097 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0015992542905707968 58-59 0.0 60-61 0.005026227770365361 62-63 0.0013707893919178257 64-65 0.0031985085811415937 66-67 0.005940087364977245 68-69 9.138595946118839E-4 70-71 0.0 72-73 0.0 74-75 9.138595946118839E-4 76-77 0.05757315446054868 78-79 0.13250964121872316 80-81 0.0018277191892237677 82-83 0.0015992542905707968 84-85 0.0 86-87 0.10920622155612013 88-89 0.3422404181821505 90-91 0.3799371264598907 92-93 2.2846489865297097E-4 94-95 2.2846489865297097E-4 96-97 0.0 98-99 0.0 100-101 0.0018277191892237677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 218852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.33606288223866 #Duplication Level Percentage of deduplicated Percentage of total 1 89.16173493404179 64.49608864878343 2 6.920579944407608 10.01215012080457 3 1.5481428703938194 3.3595968007049026 4 0.7366184517659103 2.131363145886247 5 0.4047795452403251 1.4640079318974062 6 0.2563612182179302 1.1126496720947703 7 0.18167565088070886 0.9199190914395016 8 0.11509740840728096 0.6660554697705424 9 0.09545603159561614 0.6214422153590504 >10 0.5245701932560538 6.5178511510382355 >50 0.034127845940468 1.6919560205471589 >100 0.01579992867614259 2.3557550321209155 >500 0.001895991441137111 0.9678116442848784 >1k 0.003159985735228518 3.6833530552684297 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 2040 0.9321367865041215 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1945 0.888728455760057 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1829 0.8357245992725677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1143 0.5222707583206916 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1100 0.5026227770365361 RNA PCR Primer, Index 46 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 988 0.4514466397382706 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 572 0.26136384405899876 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 557 0.25450989709940963 RNA PCR Primer, Index 46 (95% over 23bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 491 0.22435253047721745 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 454 0.20744612797689763 RNA PCR Primer, Index 46 (96% over 28bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 422 0.1928243744631075 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.16221007804360937 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 263 0.12017253669146272 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.117887887704933 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 239 0.10920622155612013 RNA PCR Primer, Index 46 (96% over 26bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0013707893919178257 0.0 22-23 0.0 0.0 0.0 0.00982399064207775 0.0 24-25 0.0 0.0 0.0 0.018277191892237678 0.0 26-27 0.0 0.0 0.0 0.027415787838356513 0.0 28-29 9.138595946118839E-4 0.0 0.0 0.06922486429185021 0.0 30-31 0.0013707893919178257 0.0 0.0 0.1567269204759381 0.0 32-33 0.0013707893919178257 0.0 0.0 0.3091130078774697 0.0 34-35 0.0013707893919178257 0.0 0.0 0.5750461499095278 0.0 36-37 0.0013707893919178257 0.0 0.0 0.9209420064701259 0.0 38-39 0.0013707893919178257 0.0 0.0 1.3669054886407253 0.0 40-41 0.0013707893919178257 0.0 0.0 1.8057865589530824 0.0 42-43 0.0013707893919178257 0.0 0.0 2.2229634638934073 0.0 44-45 0.0013707893919178257 0.0 0.0 2.7315263282949207 0.0 46-47 0.0013707893919178257 0.0 0.0 3.333074406448193 0.0 48-49 0.0013707893919178257 0.0 0.0 3.9076636265604154 0.0 50-51 0.0013707893919178257 0.0 0.0 4.481338987078026 0.0 52-53 0.0013707893919178257 0.0 0.0 5.053643558203717 0.0 54-55 0.0013707893919178257 0.0 0.0 5.702255405479502 0.0 56-57 0.0013707893919178257 0.0 0.0 6.469440535156179 0.0 58-59 0.0013707893919178257 0.0 0.0 7.195273518176667 0.0 60-61 0.0013707893919178257 0.0 0.0 8.009293952077202 0.0 62-63 0.0013707893919178257 0.0 0.0 8.794299343848811 0.0 64-65 0.0013707893919178257 0.0 0.0 9.566739166194505 0.0 66-67 0.0013707893919178257 0.0 0.0 10.488595032259244 0.0 68-69 0.0013707893919178257 0.0 0.0 11.445406027817887 0.0 70-71 0.0013707893919178257 0.0 0.0 12.47623965054009 0.0 72-73 0.0013707893919178257 0.0 0.0 13.530148228026246 0.0 74-75 0.0013707893919178257 0.0 0.0 14.56555114872151 0.0 76-77 0.0013707893919178257 0.0 0.0 15.639793102187781 0.0 78-79 0.0013707893919178257 0.0 0.0 16.807020269405808 0.0 80-81 0.0013707893919178257 0.0 0.0 17.97584669091441 0.0 82-83 0.0013707893919178257 0.0 0.0 19.16637727779504 0.0 84-85 0.0013707893919178257 0.0 0.0 20.399402335825123 0.0 86-87 0.0013707893919178257 0.0 0.0 21.60843857949665 0.0 88-89 0.0013707893919178257 0.0 0.0 22.871849469047575 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 15 6.6327496E-4 94.920456 5 GGCTTAG 40 5.334172E-4 47.579857 1 CGGGTCG 55 0.0025914845 34.50076 6 GGTATCA 1765 0.0 33.69678 1 GCTTAGA 60 0.0039602276 31.640152 2 GCCTTAG 65 0.005786147 29.279913 1 TTATCCG 65 0.0058708997 29.192947 9 TAGTAAG 65 0.0058708997 29.192947 7 GTCTTAA 105 6.802412E-5 27.188492 1 CCGGGTC 70 0.00841128 27.12013 5 GTCGGGA 70 0.00843006 27.10774 9 GTATCAA 2385 0.0 25.336025 1 TAGGGCA 95 0.0012293697 24.979069 4 TGTGGAC 115 1.2844062E-4 24.761858 3 TAGGAGG 115 1.2844062E-4 24.761858 4 GTTATAG 100 0.001633322 23.789928 1 ATGGGAT 180 1.9604158E-7 23.71927 7 GTAGACC 105 0.002201271 22.60011 3 CTAGGAG 150 3.0383395E-5 22.148106 3 CCTATAG 110 0.0028421425 21.627209 1 >>END_MODULE