FastQCFastQC Report
Thu 26 May 2016
SRR938819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4818514
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT342310.7104057391967731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246160.5108628925847263No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT198510.41197348394131467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183740.38132088025478394No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT155600.3229211329467965No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC141980.2946551571708622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126090.2616781854322723No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT102080.2118495453162531No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT88500.18366658268503527No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC77160.16013235615793583No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC76580.15892866555954804No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG70350.1459993682699687No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC69160.1435297272146558No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA69050.14330144106668571No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA62080.12883640059985296No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA61150.12690634498519668No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA50990.10582100622723105No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48590.10084021754424705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG271950.026.5999761
TACATGG277950.025.5682562
ACATGGG276550.024.7040483
GAGTACT199650.021.94765712-13
CATGGGG99800.021.267874
CATGGGA171550.021.0722224
AGTACTT205550.020.88004312-13
GTACTTT213750.020.53374714-15
TAGCGCG3001.72804E-1020.5304574
ATGGGAT70400.018.8435135
ACTTGAC22200.018.5537458
ATGGGAG64300.018.2733125
ACTTTTT246600.017.97233216-17
AGTACAT208300.017.945862
TACCTGG34100.017.645172
TACTTTT242450.017.60508514-15
GATGTGC27550.017.1847698
CCAAGAC30950.016.991783
GTCCAAG30650.016.8582231
AGAGTAC425000.016.8438610-11
Produced by FastQC (version 0.11.5)