FastQCFastQC Report
Thu 26 May 2016
SRR938819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4818514
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT383010.7948716139457102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236060.4899020735438353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196030.40682666896889785No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT177730.36884815526114484No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT140020.2905875130797586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131990.2739226242779413No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC127870.26537227037215205No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT110790.22992565757824923No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC93820.19470733093231649No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC89920.1866135493224675No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT85740.17793867569960367No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG80540.16714696688647163No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA72800.1510839233838482No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA72410.15027454522286332No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC66790.1386111983902091No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA63540.13186638038200157No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA59630.12375184548597347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.11038672918663305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG274200.023.6963461
TACATGG279800.022.69152
GAGTACT211900.022.24965312-13
ACATGGG278050.021.742093
GTACTTT223700.020.99114614-15
AGTACTT217450.020.55785612-13
CATGGGG96500.019.3741974
TATCACG2251.6861159E-618.9808122
CATGGGA176300.018.544784
GTAAGCG3200.018.53623694-95
ACTTTTT254750.018.46924416-17
TCGCGCT14650.018.13894360-61
CGCGGAA13850.018.1032382-83
ATTCGCG14550.017.9813280-81
TACTTTT250500.017.79817214-15
AGAGTGC35650.017.7030018
ACTTGAC22000.017.6866668
ATGGGAT69500.017.6835635
CTCGCGC14800.017.4741660-61
GAGTGCA32500.017.3749249
Produced by FastQC (version 0.11.5)