##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938819_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4818514 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01251983495327 33.0 31.0 34.0 30.0 34.0 2 32.21842418637779 34.0 31.0 34.0 30.0 34.0 3 32.30652520673386 34.0 31.0 34.0 30.0 34.0 4 35.92785970944569 37.0 35.0 37.0 35.0 37.0 5 35.842928130954895 37.0 35.0 37.0 35.0 37.0 6 35.89064138861068 37.0 35.0 37.0 35.0 37.0 7 35.82751964609836 37.0 35.0 37.0 35.0 37.0 8 35.8013916738646 37.0 35.0 37.0 33.0 37.0 9 37.44427120062326 39.0 37.0 39.0 34.0 39.0 10-11 37.367727685340334 39.0 37.0 39.0 34.0 39.0 12-13 37.298154472519954 39.0 37.0 39.0 34.0 39.0 14-15 38.483466894565424 40.0 38.0 41.0 34.0 41.0 16-17 38.4333289267189 40.0 38.0 41.0 33.0 41.0 18-19 38.48576957543342 40.0 38.0 41.0 33.5 41.0 20-21 38.56347703877171 40.0 38.0 41.0 34.0 41.0 22-23 38.51661342065209 40.0 38.0 41.0 34.0 41.0 24-25 38.43224269972029 40.0 38.0 41.0 34.0 41.0 26-27 38.26426124319656 40.0 38.0 41.0 33.5 41.0 28-29 38.11071971566338 40.0 37.5 41.0 33.0 41.0 30-31 37.887864080087766 40.0 37.0 41.0 33.0 41.0 32-33 37.65692389811465 40.0 36.5 41.0 33.0 41.0 34-35 37.596791874009284 40.0 37.0 41.0 32.0 41.0 36-37 37.49616147218832 40.0 36.5 41.0 32.0 41.0 38-39 37.39059791047613 40.0 36.0 41.0 32.0 41.0 40-41 37.238740097050666 40.0 36.0 41.0 31.0 41.0 42-43 37.04285574349271 40.0 36.0 41.0 31.0 41.0 44-45 36.766578866430606 40.0 35.5 41.0 30.5 41.0 46-47 36.429278611621754 39.0 35.0 41.0 30.0 41.0 48-49 36.112896216551405 39.0 35.0 41.0 29.5 41.0 50-51 35.85642015359922 39.0 35.0 41.0 28.5 41.0 52-53 35.65153084540171 39.0 35.0 40.5 28.0 41.0 54-55 35.41105400959715 38.5 35.0 40.0 27.5 41.0 56-57 35.14770923151826 38.0 34.5 40.0 27.0 41.0 58-59 34.85583989171765 38.0 34.0 40.0 26.0 41.0 60-61 34.482043945498546 37.5 34.0 40.0 26.0 41.0 62-63 34.14310615264374 37.0 33.5 40.0 26.0 41.0 64-65 33.785771401722606 36.5 33.0 39.5 25.0 41.0 66-67 33.38448503003208 36.0 33.0 39.0 23.5 40.5 68-69 32.940359932543515 35.5 32.5 39.0 22.5 40.0 70-71 32.4524774650442 35.0 32.0 38.0 22.0 40.0 72-73 32.104803576372305 35.0 31.5 37.0 21.5 39.5 74-75 31.692371341039998 35.0 31.0 37.0 20.0 39.0 76-77 29.657908434010984 33.0 28.5 35.0 17.5 37.0 78-79 30.72198472392111 34.0 30.5 36.0 18.0 38.0 80-81 30.762633459195094 34.0 31.0 35.5 18.0 37.0 82-83 30.463026775474763 34.0 31.0 35.0 15.5 37.0 84-85 30.08952158279503 34.0 30.5 35.0 10.0 36.5 86-87 29.585664895857935 34.0 30.0 35.0 4.5 36.0 88-89 29.08010010970187 34.0 29.0 35.0 2.0 35.5 90-91 28.664746122974844 34.0 29.0 35.0 2.0 35.0 92-93 28.407551269955842 34.0 29.0 35.0 2.0 35.0 94-95 28.033421507128548 34.0 29.0 35.0 2.0 35.0 96-97 27.51654918923137 33.0 27.0 35.0 2.0 35.0 98-99 26.816247810009475 33.0 26.5 35.0 2.0 35.0 100-101 23.66918047763273 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04911129086856647 1101 2 0.12082246114465534 1101 3 0.13055218099385968 1101 4 0.1313814230350019 1101 5 0.08962752035331789 1101 6 0.1505170834892411 1101 7 0.1598770594630352 1101 8 0.097073712564125 1101 9 0.22523553597311974 1101 10-11 0.20365809683509895 1101 12-13 0.15078723756297308 1101 14-15 0.21065413885005313 1101 16-17 0.20288203875556832 1101 18-19 0.13652622495238376 1101 20-21 0.1586576744111099 1101 22-23 0.15318050697982244 1101 24-25 0.18780044073145064 1101 26-27 0.1830081953178393 1101 28-29 0.233511483489643 1101 30-31 0.17683489212257797 1101 32-33 0.20397156107388525 1101 34-35 0.15202180589129455 1101 36-37 0.13883667201213967 1101 38-39 0.19508212617895282 1101 40-41 0.2598369359102719 1101 42-43 0.21078094308061424 1101 44-45 0.25081852485766376 1101 46-47 0.1408849043852527 1101 48-49 0.1298000705531308 1101 50-51 0.20171474625484365 1101 52-53 0.20542013375516888 1101 54-55 0.15497120719619062 1101 56-57 0.057189212466951744 1101 58-59 0.1959349090488658 1101 60-61 0.1347416654082778 1101 62-63 0.08672628045145103 1101 64-65 0.009376573801631594 1101 66-67 -0.03355718785653039 1101 68-69 0.03552817299448208 1101 70-71 0.014131866027234707 1101 72-73 -0.06036777028828055 1101 74-75 0.004919040051628798 1101 76-77 -0.06965182278802828 1101 78-79 0.05210643431405515 1101 80-81 0.11333357074262551 1101 82-83 0.11984778572642796 1101 84-85 0.14835997859419692 1101 86-87 0.21707689518118656 1101 88-89 0.2714266977568798 1101 90-91 0.3494694900922717 1101 92-93 0.3529927762690299 1101 94-95 0.2880874586376656 1101 96-97 0.22244131553007662 1101 98-99 0.38309872055523897 1101 100-101 1.010237415365065 1102 1 0.09468381574131257 1102 2 0.08165173679000759 1102 3 0.1325021181273982 1102 4 0.08181474759917506 1102 5 0.07286721713379052 1102 6 0.1206519070184271 1102 7 0.10461972824053589 1102 8 0.07422495814404328 1102 9 0.13879493893360006 1102 10-11 0.17557330995850862 1102 12-13 0.22603376492791227 1102 14-15 0.18771841056800298 1102 16-17 0.2519091766601633 1102 18-19 0.16143080179973168 1102 20-21 0.21337102110727102 1102 22-23 0.14039333642023166 1102 24-25 0.1042640123519476 1102 26-27 0.15728228188163484 1102 28-29 0.18583089569987266 1102 30-31 0.1325372973383807 1102 32-33 0.21308813507141622 1102 34-35 0.22297445522372783 1102 36-37 0.26895041210353554 1102 38-39 0.26844977471092335 1102 40-41 0.2999026297304468 1102 42-43 0.30705145894140884 1102 44-45 0.18520418886892998 1102 46-47 0.20963366975897912 1102 48-49 0.21243268228739254 1102 50-51 0.2226269513371406 1102 52-53 0.2391419784249038 1102 54-55 0.2634264896178209 1102 56-57 0.19475873772447727 1102 58-59 0.22424204568022077 1102 60-61 0.12681543497154735 1102 62-63 0.03768996769381516 1102 64-65 0.057847181972682904 1102 66-67 0.013673832134344366 1102 68-69 0.0444101328928852 1102 70-71 0.05751703103883443 1102 72-73 0.03651427761381143 1102 74-75 0.08038253396673767 1102 76-77 0.007772702343114446 1102 78-79 -0.04683096791056229 1102 80-81 -0.01753807774441185 1102 82-83 0.032439335312297146 1102 84-85 0.1426819148252214 1102 86-87 0.04044842898256107 1102 88-89 0.18896251011053522 1102 90-91 0.18701888684568146 1102 92-93 0.2866293790643617 1102 94-95 0.3603525332488786 1102 96-97 0.37637105487998923 1102 98-99 0.4437852710632697 1102 100-101 0.8267554743780927 1103 1 0.06958069795306443 1103 2 0.057884489805502426 1103 3 -0.004203423847819465 1103 4 0.05105134712931658 1103 5 0.058815737981440463 1103 6 -0.0181111019834006 1103 7 0.06131334722869752 1103 8 0.10692448346600258 1103 9 0.052201455142117936 1103 10-11 -0.014586526879391215 1103 12-13 0.09436293161110143 1103 14-15 0.12106406106214251 1103 16-17 -0.035405778829719736 1103 18-19 0.019978341044087244 1103 20-21 0.09568903574821519 1103 22-23 -0.06624090284550022 1103 24-25 0.037047619360848216 1103 26-27 -0.015803498042302522 1103 28-29 0.10757932897454481 1103 30-31 -0.00558459514202525 1103 32-33 0.03660574278485029 1103 34-35 -0.055988044740523435 1103 36-37 -0.14426915598473755 1103 38-39 -0.023376125695513394 1103 40-41 0.05308286930260664 1103 42-43 0.15472473773424866 1103 44-45 0.08485605535692997 1103 46-47 0.1348040353711113 1103 48-49 0.12265775979926019 1103 50-51 0.12605393844554413 1103 52-53 0.05260013077883485 1103 54-55 -0.0630139148634683 1103 56-57 -0.03253521740594323 1103 58-59 -0.18603690019342167 1103 60-61 -0.02752039626108882 1103 62-63 -0.035185139893016526 1103 64-65 -0.0019881846914557855 1103 66-67 -0.08180530249993723 1103 68-69 -0.10969096972053194 1103 70-71 -0.14281733098957972 1103 72-73 -0.09710418753819994 1103 74-75 -0.08691281214809621 1103 76-77 -0.0993039723518443 1103 78-79 -0.1727264948349223 1103 80-81 -0.09977451963580819 1103 82-83 -0.05407205457168729 1103 84-85 -0.11624462155072735 1103 86-87 -0.062240672257949825 1103 88-89 0.017160346834607765 1103 90-91 0.06454455433602746 1103 92-93 0.09190216927855133 1103 94-95 0.00955989351190567 1103 96-97 -0.10298507153466474 1103 98-99 0.025853420444299502 1103 100-101 0.6945617305426346 1104 1 -0.028083694028314454 1104 2 0.022433891309702858 1104 3 0.028763909065716575 1104 4 -0.008295866142375985 1104 5 0.0048865173511671856 1104 6 0.059176973838113156 1104 7 -0.06303249082328222 1104 8 -0.050484993822216495 1104 9 -0.016940070206274527 1104 10-11 0.06724586991249026 1104 12-13 -0.0022483459548254814 1104 14-15 0.023792650738158727 1104 16-17 0.13019928918474477 1104 18-19 0.10190056156491067 1104 20-21 0.06503330478503955 1104 22-23 -0.014217905969246658 1104 24-25 0.009910139878826385 1104 26-27 0.041327145828930156 1104 28-29 0.06028384889366123 1104 30-31 -0.041628680782871186 1104 32-33 -0.017171603183946615 1104 34-35 -0.07745596145142741 1104 36-37 -0.04301959395787236 1104 38-39 0.04213353218640492 1104 40-41 0.05856750755704354 1104 42-43 0.034485588709110004 1104 44-45 0.07779275767376959 1104 46-47 0.11920188793573772 1104 48-49 0.09922782353612547 1104 50-51 0.2064670338854242 1104 52-53 0.10775001244079618 1104 54-55 -0.017733520619870546 1104 56-57 0.04544442606686516 1104 58-59 0.01571126655181132 1104 60-61 0.08883335901676759 1104 62-63 -0.08501893073825073 1104 64-65 0.007797233721532848 1104 66-67 0.030729317962624236 1104 68-69 0.05761196920997946 1104 70-71 0.06312543785222857 1104 72-73 -0.010561788327059674 1104 74-75 0.01167199441721678 1104 76-77 0.0353316981453311 1104 78-79 0.103955792835567 1104 80-81 0.12418560636216114 1104 82-83 0.13768811839210215 1104 84-85 0.2480879747230702 1104 86-87 0.4223444249095323 1104 88-89 0.5766963514332915 1104 90-91 0.5024951002886979 1104 92-93 0.5274068667252081 1104 94-95 0.5190467259309877 1104 96-97 0.5613383308289634 1104 98-99 0.5657251023912337 1104 100-101 1.0644939458127851 1105 1 -0.049408197219346306 1105 2 -0.025742667966532906 1105 3 -0.007091025348145763 1105 4 -0.07856312136416932 1105 5 -0.02989433118847984 1105 6 -0.023608877964726105 1105 7 0.03190088825832049 1105 8 -0.008456584509538345 1105 9 0.01729969611446336 1105 10-11 0.06034260118043022 1105 12-13 -0.0071428208527279935 1105 14-15 0.060416637173432264 1105 16-17 -0.06634912183441344 1105 18-19 -0.041719824009497586 1105 20-21 0.009113714631446612 1105 22-23 -0.026105739198648337 1105 24-25 -0.06700255081838691 1105 26-27 -0.0189917214293871 1105 28-29 0.004228012403793002 1105 30-31 -0.05537907676863796 1105 32-33 0.01924960222850558 1105 34-35 0.06740530056543292 1105 36-37 0.003973183443214623 1105 38-39 -0.017188177092421597 1105 40-41 -0.055362477620157335 1105 42-43 0.026678292404646697 1105 44-45 0.07288189872276263 1105 46-47 0.20238581322808358 1105 48-49 0.17608260273264165 1105 50-51 0.1360481921335932 1105 52-53 0.06399655749331146 1105 54-55 0.13587510111364764 1105 56-57 0.09933361903652127 1105 58-59 0.08216466821708224 1105 60-61 0.1283591836429494 1105 62-63 0.02035508406277131 1105 64-65 -0.04356386808562007 1105 66-67 0.07327902155195432 1105 68-69 0.20266321362646522 1105 70-71 0.07685173430284209 1105 72-73 -0.14050180726176364 1105 74-75 -0.1312942998185882 1105 76-77 -0.11426074286073273 1105 78-79 -0.22133061039731317 1105 80-81 -0.07146331560345232 1105 82-83 -0.03040151009382086 1105 84-85 -0.021833445361327364 1105 86-87 0.09973765398401113 1105 88-89 0.11692133489039236 1105 90-91 0.25704156156015756 1105 92-93 0.28687480708180857 1105 94-95 0.21283966599258264 1105 96-97 0.184495624092623 1105 98-99 0.30646153153905686 1105 100-101 0.6200062038983845 1106 1 -0.10723545467134343 1106 2 -0.12576184287818393 1106 3 -0.13692043615172622 1106 4 -0.06493552065424524 1106 5 -0.03237955277798932 1106 6 -0.06425662988637981 1106 7 -0.12750537365567283 1106 8 -0.106385784074007 1106 9 -0.07973814012729008 1106 10-11 -0.12605482452016048 1106 12-13 -0.10767508219147004 1106 14-15 -0.02007460065713218 1106 16-17 -0.09776224080484042 1106 18-19 -0.19174244230317328 1106 20-21 -0.07588520284126332 1106 22-23 -0.11949541346371007 1106 24-25 -0.17574005392981462 1106 26-27 -0.10975940657193206 1106 28-29 -0.08776151071256066 1106 30-31 -0.04103270944664672 1106 32-33 -0.04649666867877755 1106 34-35 -0.05443436116392064 1106 36-37 0.016935311192234792 1106 38-39 -0.0872591782793748 1106 40-41 -0.09364028247986766 1106 42-43 -0.03893818775674873 1106 44-45 0.04064491121841485 1106 46-47 -0.017294622915635216 1106 48-49 -0.0987880003723447 1106 50-51 0.04273762563198602 1106 52-53 0.0022624793091878814 1106 54-55 -0.03276289050790382 1106 56-57 -0.161434196639469 1106 58-59 -0.14724766575226766 1106 60-61 -0.11240604837123414 1106 62-63 -0.1718740354261854 1106 64-65 -0.07395389749164849 1106 66-67 -0.09273401018612049 1106 68-69 -0.08570983563009804 1106 70-71 -0.050096983611254586 1106 72-73 0.010385775092451865 1106 74-75 0.185581242851665 1106 76-77 0.03051465635777717 1106 78-79 0.041960754185726756 1106 80-81 -0.13918930611592373 1106 82-83 0.017115338847037975 1106 84-85 -0.1536344511889709 1106 86-87 0.005664682192286108 1106 88-89 0.13294102833486932 1106 90-91 0.16781351829687452 1106 92-93 0.22267072120521192 1106 94-95 0.2989003604394682 1106 96-97 0.18422199900355807 1106 98-99 0.261772171859576 1106 100-101 0.630048433715551 1107 1 -0.08149975256519326 1107 2 -0.11506032068118799 1107 3 -0.15195928767504796 1107 4 -0.06918012284646835 1107 5 -0.06214791563724731 1107 6 -0.06544621677024764 1107 7 -0.12184915069098423 1107 8 -0.06975477592644808 1107 9 -0.19965860809963232 1107 10-11 -0.1333120008467148 1107 12-13 -0.15324343914510052 1107 14-15 -0.1851978938101837 1107 16-17 -0.12531458381766925 1107 18-19 -0.054380128214695844 1107 20-21 -0.11580738520785872 1107 22-23 -0.2031176376544721 1107 24-25 -0.24718047123717213 1107 26-27 -0.2122118717959509 1107 28-29 -0.26745695672610736 1107 30-31 -0.27463961867585596 1107 32-33 -0.15623793176866485 1107 34-35 -0.19762134075822502 1107 36-37 -0.14527542884874123 1107 38-39 -0.13208016510939302 1107 40-41 -0.18531271153854334 1107 42-43 -0.1789097085390381 1107 44-45 -0.22453142238419588 1107 46-47 -0.2674943564573411 1107 48-49 -0.46724660568396814 1107 50-51 -0.3653408513160201 1107 52-53 -0.4093496550764044 1107 54-55 -0.4272076361098698 1107 56-57 -0.43890079790353553 1107 58-59 -0.4852102822616615 1107 60-61 -0.4196374867480088 1107 62-63 -0.45831694499563724 1107 64-65 -0.41038418048128733 1107 66-67 -0.4562056012629867 1107 68-69 -0.368972345529734 1107 70-71 -0.4548970671714514 1107 72-73 -0.320569111658358 1107 74-75 -0.28120126662487976 1107 76-77 -0.29483149482711823 1107 78-79 -0.22880576074162562 1107 80-81 -0.2897341000646847 1107 82-83 -0.18858242181911677 1107 84-85 -0.24466530455626057 1107 86-87 -0.3361010469342993 1107 88-89 -0.11193297690879689 1107 90-91 0.027554935638171685 1107 92-93 0.13880491200010425 1107 94-95 0.12818613674054546 1107 96-97 0.10784383976938372 1107 98-99 -0.03539583833688553 1107 100-101 0.530662288224736 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-2.082500225746667 2316 98-99 -2.6064226295787165 2316 100-101 -3.286277623846452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 15.0 4 48.0 5 154.0 6 376.0 7 874.0 8 1924.0 9 3413.0 10 5635.0 11 8513.0 12 11905.0 13 16810.0 14 20182.0 15 26908.0 16 35088.0 17 47440.0 18 44065.0 19 29639.0 20 26868.0 21 28748.0 22 29385.0 23 32249.0 24 37788.0 25 44759.0 26 54837.0 27 66746.0 28 82448.0 29 100484.0 30 122078.0 31 148575.0 32 182835.0 33 231449.0 34 308484.0 35 436668.0 36 654791.0 37 1009061.0 38 876278.0 39 90983.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.684456699623006 19.56775147220136 14.817288691055955 21.930503137119683 2 15.704115418155887 21.754528470810712 38.625684183962115 23.91567192707129 3 20.340378797280657 25.339160579381943 29.909885080753114 24.41057554258429 4 12.86724496390381 17.181853160538704 37.30206034474529 32.64884153081219 5 13.309954064676372 38.40391456785225 33.53245419645974 14.753677171011645 6 30.901746887110836 36.68834416585695 17.180794742943572 15.229114204088646 7 28.200374638322106 33.70205005111534 20.394295834773956 17.703279475788595 8 26.4124167741341 33.559724014499075 19.520603239920025 20.5072559714468 9 26.448776531519886 17.665861300807677 20.81135387382915 35.07400829384329 10-11 25.365403109755412 26.472404147834787 26.65022868046041 21.51196406194939 12-13 27.183785291481982 24.095270865665224 27.009385051075913 21.711558791776884 14-15 23.21202712416093 25.45570896056321 26.94708331426623 24.38518060100963 16-17 21.85215043693405 28.963643463308237 27.11282334224949 22.071382757508218 18-19 21.494697423911596 27.67543971607803 29.51637301761728 21.31348984239309 20-21 22.973768048358487 25.93528335401399 29.151400027352835 21.939548570274688 22-23 22.217440895678624 26.081972574947383 29.324953709795174 22.37563281957882 24-25 22.689993221976735 26.307861718363796 29.42961253199638 21.572532527663093 26-27 22.37791582646044 26.06506223553155 30.12510403891494 21.43191789909307 28-29 21.329719079367624 27.279582460484708 29.87411679202343 21.51658166812424 30-31 21.767924717039318 26.319141129402134 29.58912228956894 22.323811863989604 32-33 21.77874755578172 26.618963854831595 29.561541172236915 22.04074741714977 34-35 22.877785116951433 26.225773160036592 29.204188552307354 21.692253170704625 36-37 22.691579527903258 25.837230594937544 29.744759894713223 21.72642998244598 38-39 22.050621703359482 26.552909283634424 29.765048496535783 21.63142051647031 40-41 22.044203430130057 26.541640260468967 29.63916378373425 21.77499252566672 42-43 21.74136825705236 26.88745667862581 28.698761754285545 22.67241331003628 44-45 22.149063231242575 26.38712857417102 28.646113615963724 22.81769457862268 46-47 22.680328072986278 27.033614613858425 28.220534031761318 22.06552328139398 48-49 22.72580301727877 26.83924961097965 28.169493748487607 22.265453623253975 50-51 21.826373551570263 27.08651292291741 28.700585701673592 22.386527823838733 52-53 22.456664025465113 27.630956348783048 27.652934078846712 22.259445546905123 54-55 21.73055842527385 26.816846438549312 28.119966031021182 23.332629105155654 56-57 22.500567602376996 27.053745200283743 27.694523664349628 22.751163532989633 58-59 23.215149202095755 26.55235096081808 27.650006288266592 22.58249354881957 60-61 22.39952997718476 26.887803740155498 28.769991093681952 21.942675188977788 62-63 22.046215908057963 26.900118999342954 28.542502937627656 22.51116215497143 64-65 21.656842752765687 27.474445441063367 28.10608208256736 22.762629723603585 66-67 22.05414757948648 27.096608232379594 27.46580287772382 23.38344131041011 68-69 21.651082552052255 27.046845734386913 28.077773569438463 23.22429814412236 70-71 22.401047312852025 27.059663564443703 27.676483183877558 22.86280593882671 72-73 22.27831028404193 26.54342189313967 28.240013414924185 22.938254407894217 74-75 21.60970166320986 27.93716070971258 27.461951962783548 22.99118566429401 76-77 22.014846579082644 26.98682084877926 27.5865256705261 23.411806901611993 78-79 22.07819196381536 27.028989170413325 27.840328446980035 23.052490418791283 80-81 22.32786107167698 27.43074186676036 27.4718645075914 22.76953255397126 82-83 22.222826373442103 27.04259030896247 27.767077152831764 22.96750616476366 84-85 21.81538748253092 27.168189196918384 27.528393608485935 23.488029712064755 86-87 21.962196313164153 27.11451505977424 27.56885601752348 23.354432609538126 88-89 21.861773802951248 27.18765522147472 27.118838524066113 23.831732451507914 90-91 22.00846391327418 26.762195713971888 27.515658197931774 23.713682174822154 92-93 22.28307316322003 27.067027303438362 27.48547581266756 23.164423720674048 94-95 21.958247285002656 26.707834969003923 27.546278281287194 23.787639464706228 96-97 21.395880555706594 26.669425470176073 28.084322262008577 23.850371712108753 98-99 21.72509623310784 27.07212481539929 26.963014277448536 24.23976467404433 100-101 21.592573257305183 26.24328405740526 27.82175950354453 24.342383181745024 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3833.0 1 2499.0 2 1395.5 3 1658.5 4 1444.5 5 1447.0 6 2742.5 7 5688.0 8 8583.0 9 9597.5 10 8254.5 11 6578.5 12 6473.0 13 6846.0 14 7276.5 15 7642.0 16 7783.0 17 8085.0 18 8489.0 19 8833.0 20 9497.0 21 10759.5 22 12538.0 23 14767.0 24 17309.0 25 20627.0 26 25911.0 27 32079.0 28 37023.5 29 43116.0 30 51347.0 31 60300.5 32 72033.5 33 82736.5 34 92264.0 35 100650.0 36 110786.0 37 122167.0 38 126810.0 39 127653.0 40 128437.5 41 135617.0 42 144361.5 43 148662.5 44 151482.0 45 155525.0 46 157653.5 47 183838.0 48 257747.5 49 258066.0 50 191622.5 51 162705.0 52 151324.5 53 150793.5 54 151748.5 55 143170.5 56 128319.5 57 115188.5 58 106539.5 59 96180.0 60 84380.0 61 72720.0 62 60914.5 63 48582.5 64 38264.5 65 30676.0 66 21898.5 67 17437.5 68 12184.0 69 6427.5 70 4804.5 71 4012.5 72 3403.5 73 2898.5 74 2355.0 75 2030.5 76 1377.5 77 662.0 78 374.0 79 219.0 80 120.5 81 74.5 82 43.0 83 24.5 84 14.5 85 10.5 86 4.5 87 2.5 88 4.5 89 2.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3886882968483644 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1414307398505015E-4 16-17 0.0022724848366114533 18-19 1.4527300325370022E-4 20-21 2.075328617910003E-5 22-23 0.0 24-25 0.0 26-27 9.338978780595012E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007917378677326661 36-37 0.016913928235966526 38-39 0.011227527822893116 40-41 0.00744005309520736 42-43 0.003424292219551505 44-45 0.0018470424699399027 46-47 0.0015357431772534023 48-49 0.0 50-51 4.150657235820006E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.3205257886560047E-4 60-61 5.084555113879508E-4 62-63 0.0 64-65 0.0 66-67 0.0010999241674923014 68-69 8.093781609849011E-4 70-71 0.0018159125406712524 72-73 0.0 74-75 0.0 76-77 0.09987518973691889 78-79 0.0345230915589329 80-81 1.0376643089550015E-5 82-83 0.0 84-85 0.0 86-87 0.15294134249687766 88-89 0.32998762689078004 90-91 0.28561710103986415 92-93 0.0 94-95 2.075328617910003E-5 96-97 0.0 98-99 1.2451971707460016E-4 100-101 7.36741659358051E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4818514.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.887521942590368 #Duplication Level Percentage of deduplicated Percentage of total 1 63.82529553731228 20.352305119383605 2 15.659933199314999 9.987129270253128 3 6.924955564667466 6.624590175593914 4 3.8134957548344923 4.8641171824104035 5 2.3556143027139993 3.755735138303619 6 1.598087155226258 3.0575423537069475 7 1.086846819854839 2.425979627144902 8 0.805521473378395 2.0548866926065035 9 0.5862331145269685 1.6824169172656618 >10 2.9172693518032453 16.339413089364584 >50 0.21855954053583798 4.857403975538427 >100 0.18307287403577396 11.507488460534292 >500 0.017005159197569623 3.6814557103537022 >1k 0.006932872382052537 4.133902656864554 >5k 6.540445643445768E-4 1.5399550199914454 >10k+ 5.232356514756614E-4 3.135678610684387 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38301 0.7948716139457102 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23606 0.4899020735438353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19603 0.40682666896889785 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17773 0.36884815526114484 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14002 0.2905875130797586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13199 0.2739226242779413 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12787 0.26537227037215205 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11079 0.22992565757824923 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9382 0.19470733093231649 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8992 0.1866135493224675 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8574 0.17793867569960367 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8054 0.16714696688647163 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7280 0.1510839233838482 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7241 0.15027454522286332 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6679 0.1386111983902091 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6354 0.13186638038200157 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5963 0.12375184548597347 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5319 0.11038672918663305 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.075328617910003E-5 0.0 4 0.0 0.0 0.0 2.075328617910003E-5 0.0 5 0.0 0.0 0.0 2.075328617910003E-5 0.0 6 0.0 0.0 0.0 2.075328617910003E-5 0.0 7 0.0 0.0 0.0 2.075328617910003E-5 0.0 8 0.0 0.0 0.0 4.150657235820006E-5 0.0 9 0.0 0.0 0.0 4.150657235820006E-5 0.0 10-11 0.0 0.0 0.0 8.301314471640012E-5 0.0 12-13 0.0 0.0 0.0 1.8677957561190025E-4 0.0 14-15 0.0 0.0 0.0 2.3866279105965035E-4 0.0 16-17 0.0 0.0 0.0 2.801693634178504E-4 0.0 18-19 0.0 0.0 0.0 3.528058650447005E-4 0.0 20-21 0.0 0.0 0.0 6.12221942283451E-4 0.0 22-23 0.0 0.0 0.0 0.0011829373122087018 0.0 24-25 0.0 0.0 0.0 0.002054575331730903 0.0 26-27 0.0 0.0 0.0 0.003351655717924655 0.0 28-29 0.0 0.0 0.0 0.00714950708869996 0.0 30-31 0.0 0.0 0.0 0.017692176467682775 0.0 32-33 0.0 0.0 0.0 0.0391303210906931 0.0 34-35 0.0 0.0 0.0 0.07423450466264081 0.0 36-37 0.0 0.0 0.0 0.12772609978927113 0.0 38-39 0.0 2.075328617910003E-5 0.0 0.20521887038203063 0.0 40-41 0.0 2.075328617910003E-5 0.0 0.29263171176839997 0.0 42-43 0.0 2.075328617910003E-5 0.0 0.3835726118052163 0.0 44-45 0.0 2.075328617910003E-5 0.0 0.49266226060565566 0.0 46-47 0.0 2.075328617910003E-5 0.0 0.6271020484738656 0.0 48-49 0.0 2.075328617910003E-5 0.0 0.7715137903511331 0.0 50-51 0.0 2.075328617910003E-5 0.0 0.9274332294147116 0.0 52-53 0.0 2.075328617910003E-5 0.0 1.0812669632172907 0.0 54-55 0.0 2.075328617910003E-5 0.0 1.2493997111972694 0.0 56-57 0.0 2.075328617910003E-5 0.0 1.4581362635866575 0.0 58-59 0.0 2.075328617910003E-5 0.0 1.6869827502835937 0.0 60-61 0.0 2.075328617910003E-5 0.0 1.9432754579523894 0.0 62-63 0.0 2.075328617910003E-5 0.0 2.1943383375040524 0.0 64-65 0.0 2.075328617910003E-5 0.0 2.4464907645801173 1.0376643089550015E-5 66-67 0.0 2.075328617910003E-5 0.0 2.7359368469200254 2.075328617910003E-5 68-69 0.0 2.075328617910003E-5 0.0 3.0716938873685953 2.075328617910003E-5 70-71 0.0 2.075328617910003E-5 0.0 3.4413202908614564 2.075328617910003E-5 72-73 0.0 2.075328617910003E-5 0.0 3.8087779759486016 2.075328617910003E-5 74-75 0.0 2.075328617910003E-5 0.0 4.188189553874908 2.075328617910003E-5 76-77 0.0 2.075328617910003E-5 0.0 4.586808298159972 2.075328617910003E-5 78-79 0.0 2.075328617910003E-5 0.0 5.051380985922216 2.075328617910003E-5 80-81 0.0 2.075328617910003E-5 0.0 5.562783463947599 2.075328617910003E-5 82-83 0.0 2.075328617910003E-5 0.0 6.067109071385909 2.075328617910003E-5 84-85 0.0 2.075328617910003E-5 0.0 6.583814014030052 2.075328617910003E-5 86-87 0.0 2.075328617910003E-5 0.0 7.111144639197894 2.075328617910003E-5 88-89 0.0 2.075328617910003E-5 0.0 7.677564078884071 2.075328617910003E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 27420 0.0 23.696346 1 TACATGG 27980 0.0 22.6915 2 GAGTACT 21190 0.0 22.249653 12-13 ACATGGG 27805 0.0 21.74209 3 GTACTTT 22370 0.0 20.991146 14-15 AGTACTT 21745 0.0 20.557856 12-13 CATGGGG 9650 0.0 19.374197 4 TATCACG 225 1.6861159E-6 18.980812 2 CATGGGA 17630 0.0 18.54478 4 GTAAGCG 320 0.0 18.536236 94-95 ACTTTTT 25475 0.0 18.469244 16-17 TCGCGCT 1465 0.0 18.138943 60-61 CGCGGAA 1385 0.0 18.10323 82-83 ATTCGCG 1455 0.0 17.98132 80-81 TACTTTT 25050 0.0 17.798172 14-15 AGAGTGC 3565 0.0 17.703001 8 ACTTGAC 2200 0.0 17.686666 8 ATGGGAT 6950 0.0 17.683563 5 CTCGCGC 1480 0.0 17.47416 60-61 GAGTGCA 3250 0.0 17.374924 9 >>END_MODULE