FastQCFastQC Report
Thu 26 May 2016
SRR938818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4832134
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT343480.7108246584221382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246030.5091539266088234No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT200100.4141027546007623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186110.38515074292227824No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT154770.3202932700127935No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC139650.28900274702646905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124680.2580226458951676No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT102120.2113351988996994No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT87690.181472616446481No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC79260.16402690819418503No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC76650.15862556791678376No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA70320.14552576563481062No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC70060.14498770108610398No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG67140.13894482230832175No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA62720.12979772498030892No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA60510.12522417631630248No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA50280.10405340580372976No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48730.10084571330182482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG271050.026.1477571
TACATGG276850.025.2929762
ACATGGG276700.024.3465443
GTAAGCG5000.022.80716994-95
GAGTACT198250.022.14673812-13
CCGTCGT2806.730261E-1122.0236539
AGTACTT203150.021.32049812-13
CATGGGA174700.021.1273384
GTACTTT212300.020.63667514-15
ACCGTCG3503.6379788E-1220.3310018
CATGGGG99500.020.2162634
GTATAGG16250.019.8912331
TACCTGG33350.019.2041932
ATGGGAG68000.019.0463545
ACTTGAC22450.018.5952458
ACTTTTT242250.018.51724216-17
GCGTATC1300.007535218.2823851
TACTTTT240000.017.9977514-15
TCGCGCT12600.017.91740460-61
CCAAGAC29950.017.582653
Produced by FastQC (version 0.11.5)