##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938818_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4832134 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.496555559096663 33.0 31.0 34.0 30.0 34.0 2 31.55221854360827 33.0 31.0 34.0 28.0 34.0 3 31.56373622916914 33.0 31.0 34.0 28.0 34.0 4 35.109481028464856 37.0 35.0 37.0 32.0 37.0 5 35.14774507495032 37.0 35.0 37.0 32.0 37.0 6 35.21812412486906 37.0 35.0 37.0 32.0 37.0 7 35.127642983410645 37.0 35.0 37.0 32.0 37.0 8 35.09425938932985 37.0 35.0 37.0 32.0 37.0 9 36.641703851755764 39.0 37.0 39.0 32.0 39.0 10-11 36.51451843015943 39.0 37.0 39.0 32.0 39.0 12-13 36.40201885957633 39.0 37.0 39.0 32.0 39.0 14-15 37.506023115253015 40.0 37.0 41.0 32.0 41.0 16-17 37.40565638287349 40.0 37.0 41.0 32.0 41.0 18-19 37.370106044244636 40.0 37.0 41.0 32.0 41.0 20-21 37.26566264511705 40.0 37.0 41.0 31.0 41.0 22-23 37.284900936108144 40.0 37.0 41.0 31.0 41.0 24-25 37.20346393539583 40.0 37.0 41.0 31.0 41.0 26-27 37.05919920681008 40.0 37.0 41.0 31.0 41.0 28-29 36.895172712511695 40.0 36.0 41.0 30.0 41.0 30-31 36.6641576992691 39.0 36.0 41.0 30.0 41.0 32-33 36.46240015281033 39.0 35.5 41.0 30.0 41.0 34-35 36.14232252251283 39.0 35.0 41.0 29.5 41.0 36-37 35.91045529780424 39.0 35.0 41.0 28.0 41.0 38-39 35.732883028492175 39.0 35.0 40.0 27.0 41.0 40-41 35.63050031311218 39.0 35.0 40.5 27.0 41.0 42-43 35.59354966149532 39.0 35.0 41.0 26.5 41.0 44-45 35.388427349076 39.0 35.0 41.0 26.0 41.0 46-47 35.026919162423894 39.0 35.0 41.0 24.0 41.0 48-49 34.69768563951248 38.5 34.0 40.5 23.0 41.0 50-51 33.54076884043364 37.5 32.5 39.5 21.5 40.5 52-53 33.619355444199186 37.5 33.0 39.5 22.0 40.5 54-55 34.10996849425119 38.0 34.0 40.0 22.0 41.0 56-57 33.98517197577716 38.0 34.0 40.0 20.5 41.0 58-59 33.78001531000589 38.0 33.0 40.0 20.0 41.0 60-61 33.436674045049244 37.5 33.0 40.0 18.5 41.0 62-63 33.05732374143598 37.0 32.5 40.0 17.5 41.0 64-65 32.664357300521885 36.0 32.0 39.5 13.5 41.0 66-67 32.249521846869314 36.0 31.5 39.0 10.5 41.0 68-69 31.824976190643717 35.0 31.0 39.0 8.0 40.0 70-71 31.38031519821263 35.0 31.0 38.0 7.0 40.0 72-73 30.95780715104341 35.0 30.5 37.0 3.5 39.5 74-75 30.498566678821405 35.0 30.0 37.0 2.0 39.0 76-77 30.04977210896883 34.5 30.0 36.0 2.0 39.0 78-79 29.607521231820144 34.0 29.0 36.0 2.0 38.5 80-81 29.12272300395643 34.0 29.0 35.0 2.0 37.0 82-83 28.712217935181435 34.0 29.0 35.0 2.0 37.0 84-85 28.356549094044162 34.0 28.5 35.0 2.0 36.0 86-87 27.957958119538905 34.0 27.0 35.0 2.0 36.0 88-89 27.484654399070887 34.0 26.5 35.0 2.0 35.5 90-91 27.015434692001506 33.0 25.5 35.0 2.0 35.0 92-93 26.55244960094236 33.0 25.0 35.0 2.0 35.0 94-95 26.025602145966978 33.0 23.5 35.0 2.0 35.0 96-97 25.39641564161921 32.5 19.5 35.0 2.0 35.0 98-99 24.619960766816483 32.0 9.5 35.0 2.0 35.0 100-101 22.11062751984941 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14704675034425208 1101 2 0.28804017720879216 1101 3 0.4036708411283918 1101 4 0.23075506805237467 1101 5 0.18422768099038223 1101 6 0.27000620823429244 1101 7 0.35146108781711405 1101 8 0.3795145973449294 1101 9 0.4743587452478195 1101 10-11 0.3353477441024424 1101 12-13 0.37928544228937966 1101 14-15 0.3725186170410666 1101 16-17 0.5894999164467691 1101 18-19 0.5675415472612144 1101 20-21 0.6725121416411568 1101 22-23 0.553231410790751 1101 24-25 0.5904596311115498 1101 26-27 0.5737510598998412 1101 28-29 0.633158527804035 1101 30-31 0.6493276685785574 1101 32-33 0.5504229687083537 1101 34-35 0.5613288140728656 1101 36-37 0.6736184253062873 1101 38-39 0.6672793743011809 1101 40-41 0.8490326729549409 1101 42-43 0.8613986088192291 1101 44-45 0.9489760569460657 1101 46-47 0.8975584021067746 1101 48-49 0.8760905153935994 1101 50-51 0.8577214548286349 1101 52-53 0.8663588958064707 1101 54-55 0.9235562200041727 1101 56-57 0.9200599991166243 1101 58-59 1.0103069905198723 1101 60-61 0.8774954954774614 1101 62-63 0.8206523423294456 1101 64-65 0.7623673332596823 1101 66-67 0.7541975151221862 1101 68-69 0.8207238352963735 1101 70-71 0.7531234872148325 1101 72-73 0.6773136087186309 1101 74-75 0.597077020035357 1101 76-77 0.6033692434518798 1101 78-79 0.5570942822765375 1101 80-81 0.6456591247357366 1101 82-83 0.6982077095001387 1101 84-85 0.7242185157874879 1101 86-87 0.5581771623192502 1101 88-89 0.6592487052231704 1101 90-91 0.5646778697109944 1101 92-93 0.47733074008185383 1101 94-95 0.4489370857413384 1101 96-97 0.43480177479396787 1101 98-99 0.4420159922186748 1101 100-101 0.7521691348226511 1102 1 0.2470500448557722 1102 2 0.21793595489159046 1102 3 0.22048482588459706 1102 4 0.29768017579181105 1102 5 0.20571328308462 1102 6 0.3041300907716007 1102 7 0.302481540500807 1102 8 0.311302804774499 1102 9 0.4432455565168283 1102 10-11 0.45412904196189885 1102 12-13 0.47926870260921817 1102 14-15 0.4183223665513438 1102 16-17 0.5966302410897129 1102 18-19 0.5106327117374718 1102 20-21 0.5390117899297948 1102 22-23 0.5050938115101999 1102 24-25 0.4880467798453978 1102 26-27 0.5636340379601776 1102 28-29 0.6415078884907146 1102 30-31 0.632337338415077 1102 32-33 0.5359300786203889 1102 34-35 0.5882601546719357 1102 36-37 0.6878933433886942 1102 38-39 0.621036119145721 1102 40-41 0.6966193764845272 1102 42-43 0.7110084095457978 1102 44-45 0.6864533960492523 1102 46-47 0.6477984339833966 1102 48-49 0.6345400915591384 1102 50-51 0.5929991064652071 1102 52-53 0.6105766496619367 1102 54-55 0.4886120931387623 1102 56-57 0.5526672933432195 1102 58-59 0.40597297157197687 1102 60-61 0.4530595470143126 1102 62-63 0.25289016154090405 1102 64-65 0.17048901648794867 1102 66-67 0.18431195487714547 1102 68-69 0.3381113321799454 1102 70-71 0.38033572944156546 1102 72-73 0.2725234780066579 1102 74-75 0.17239551916275886 1102 76-77 0.2345518617871747 1102 78-79 0.18367827451217522 1102 80-81 0.19017977330930336 1102 82-83 0.14856467427557973 1102 84-85 0.12850863759537035 1102 86-87 0.18969987218853746 1102 88-89 0.2921051134932853 1102 90-91 0.16576735131515008 1102 92-93 0.11372276024070516 1102 94-95 0.026024114270025933 1102 96-97 0.05946629112406043 1102 98-99 -0.11907807498936407 1102 100-101 0.13196002238186466 1103 1 0.25246793881547447 1103 2 0.3275403893953097 1103 3 0.3637607795199109 1103 4 0.35349075626642446 1103 5 0.34808026378563284 1103 6 0.2828654109655844 1103 7 0.35333246488283265 1103 8 0.4066363031403526 1103 9 0.4296941912362513 1103 10-11 0.4188046167494406 1103 12-13 0.47752567568746684 1103 14-15 0.5722280428338777 1103 16-17 0.6941966730394284 1103 18-19 0.6899580922482969 1103 20-21 0.6820376647748034 1103 22-23 0.6540697556579573 1103 24-25 0.6853736238384087 1103 26-27 0.6790799038410853 1103 28-29 0.6881447693325171 1103 30-31 0.8103328694508249 1103 32-33 0.6820116214054295 1103 34-35 0.7276950455269073 1103 36-37 0.7844133746649078 1103 38-39 0.8243762827896362 1103 40-41 0.8780010258136883 1103 42-43 0.9640533358318777 1103 44-45 0.8699023502919871 1103 46-47 0.7841252463512376 1103 48-49 0.8095738050852219 1103 50-51 0.8050306313111406 1103 52-53 0.798996339713419 1103 54-55 0.7992445420940939 1103 56-57 0.9098270473647929 1103 58-59 0.8373107273405722 1103 60-61 0.7462548782661926 1103 62-63 0.6439689398495219 1103 64-65 0.5942634482980793 1103 66-67 0.5335678799625612 1103 68-69 0.6395583910932707 1103 70-71 0.5716971713750176 1103 72-73 0.5549911794344844 1103 74-75 0.4345804915343514 1103 76-77 0.5952562307504436 1103 78-79 0.5416266731333153 1103 80-81 0.7033300659512136 1103 82-83 0.7176625389220135 1103 84-85 0.7554725009266363 1103 86-87 0.6900761245181677 1103 88-89 0.8219796706045344 1103 90-91 0.7575605750218166 1103 92-93 0.5112625616127993 1103 94-95 0.5410270096092411 1103 96-97 0.5442272975025304 1103 98-99 0.7242100052644211 1103 100-101 1.0295589519242476 1104 1 0.044065491615594254 1104 2 0.05639288252933028 1104 3 0.003322539556105397 1104 4 0.16497478242819597 1104 5 0.19002238297714058 1104 6 0.21619685336651884 1104 7 0.24825738911908957 1104 8 0.2080781419680804 1104 9 0.2955409432926004 1104 10-11 0.2625514484318572 1104 12-13 0.33619614531231434 1104 14-15 0.39089595969998214 1104 16-17 0.4544788550420691 1104 18-19 0.4266624307139324 1104 20-21 0.4737261288916912 1104 22-23 0.3734260035285075 1104 24-25 0.37516448987624784 1104 26-27 0.29380683883393033 1104 28-29 0.33136802683544175 1104 30-31 0.37455447287643295 1104 32-33 0.39405330163622665 1104 34-35 0.2781387996564959 1104 36-37 0.427150715705892 1104 38-39 0.5042104629618152 1104 40-41 0.5211772758292099 1104 42-43 0.4209590223886295 1104 44-45 0.5575936347718979 1104 46-47 0.6061711932497218 1104 48-49 0.577466264368681 1104 50-51 0.6194805044749856 1104 52-53 0.6879324755695038 1104 54-55 0.585567837565101 1104 56-57 0.5712952448705622 1104 58-59 0.5210439425854716 1104 60-61 0.5468251064609291 1104 62-63 0.47501864182938647 1104 64-65 0.5952959103196491 1104 66-67 0.5270497023547023 1104 68-69 0.5361428671475217 1104 70-71 0.47229795335890046 1104 72-73 0.5221793281852278 1104 74-75 0.35997713041063406 1104 76-77 0.4248041501960351 1104 78-79 0.25192987379791276 1104 80-81 0.2344612432652653 1104 82-83 0.2970548228487928 1104 84-85 0.3097009289514254 1104 86-87 0.27852647754202664 1104 88-89 0.41861652519884274 1104 90-91 0.3703160190104491 1104 92-93 0.4349629651573146 1104 94-95 0.6419551456142933 1104 96-97 0.5907659816687705 1104 98-99 0.8201984324132923 1104 100-101 1.1587344927539043 1105 1 -5.926272871903393E-5 1105 2 0.10748129085416736 1105 3 0.11922902072461738 1105 4 0.19774597728675758 1105 5 0.23143995656027982 1105 6 0.21120249459632845 1105 7 0.1905079709439761 1105 8 0.23784564915237638 1105 9 0.2700721183517487 1105 10-11 0.3018901962658802 1105 12-13 0.35690075712623326 1105 14-15 0.444004848461212 1105 16-17 0.39941722485691855 1105 18-19 0.3999988732922901 1105 20-21 0.4375735361303654 1105 22-23 0.3813565823340497 1105 24-25 0.4262277857104877 1105 26-27 0.3736580877200453 1105 28-29 0.4191608347183333 1105 30-31 0.4497462170752158 1105 32-33 0.42182122414730827 1105 34-35 0.3523288205915094 1105 36-37 0.5764849695939631 1105 38-39 0.5383556429668133 1105 40-41 0.49815116768993306 1105 42-43 0.46097428360194925 1105 44-45 0.46306606605845246 1105 46-47 0.6180496309263219 1105 48-49 0.7090525003691468 1105 50-51 0.767246323632321 1105 52-53 0.6958541407977421 1105 54-55 0.6704609355922386 1105 56-57 0.6028121094423042 1105 58-59 0.6151656199712434 1105 60-61 0.6567947694032981 1105 62-63 0.6600889718329768 1105 64-65 0.6997985443965504 1105 66-67 0.6042118690077842 1105 68-69 0.5616802869712494 1105 70-71 0.4934250937864455 1105 72-73 0.5898307287399298 1105 74-75 0.6536846792378306 1105 76-77 0.6171808163461172 1105 78-79 0.6305356141249945 1105 80-81 0.7551266937887746 1105 82-83 0.6363943973283881 1105 84-85 0.6431091126758268 1105 86-87 0.7706247687069734 1105 88-89 0.8119748413201364 1105 90-91 0.8478119083274471 1105 92-93 0.9174589120890069 1105 94-95 0.9526334149891973 1105 96-97 0.8076735014047252 1105 98-99 0.7506783327348678 1105 100-101 1.2153990429852293 1106 1 0.1486997385084905 1106 2 0.15423388022698958 1106 3 0.184161826305278 1106 4 0.21940688319370594 1106 5 0.1720210381900671 1106 6 0.24205305203147987 1106 7 0.20334614374879578 1106 8 0.1967452485552954 1106 9 0.2189461983674903 1106 10-11 0.21946302505969584 1106 12-13 0.23100763474631236 1106 14-15 0.3625882576658128 1106 16-17 0.32663344826045204 1106 18-19 0.28755970070397296 1106 20-21 0.3148043359651851 1106 22-23 0.26249144236901145 1106 24-25 0.2283636441487502 1106 26-27 0.19195518820987445 1106 28-29 0.18220022085357357 1106 30-31 0.023640699280093713 1106 32-33 0.19020371692649718 1106 34-35 0.17510848465231987 1106 36-37 0.3233145656956111 1106 38-39 0.34470847541657434 1106 40-41 0.5089186888171398 1106 42-43 0.538901277547815 1106 44-45 0.5944212576994019 1106 46-47 0.5794142440855694 1106 48-49 0.6084073979938864 1106 50-51 0.6991028313649323 1106 52-53 0.4641010803891348 1106 54-55 0.4008928288720597 1106 56-57 0.5527374137579031 1106 58-59 0.6279659574479339 1106 60-61 0.6812554093475427 1106 62-63 0.6415598521114063 1106 64-65 0.6633473553401146 1106 66-67 0.5257403954007884 1106 68-69 0.6439033159191716 1106 70-71 0.6578795573039606 1106 72-73 0.6503594499668246 1106 74-75 0.4389127400035342 1106 76-77 0.5927011784932006 1106 78-79 0.6968093270056457 1106 80-81 0.6842861805700053 1106 82-83 0.7450545508619868 1106 84-85 0.9391998245401041 1106 86-87 1.0017180780888992 1106 88-89 1.220993495621432 1106 90-91 1.2294944377463217 1106 92-93 1.302606643999507 1106 94-95 1.2906063906948226 1106 96-97 1.2765078635540519 1106 98-99 1.4392942442115917 1106 100-101 1.805815145732982 1107 1 0.026196702374605252 1107 2 0.01523854257171564 1107 3 0.08849301710225532 1107 4 0.13743142716973722 1107 5 0.13492434499357842 1107 6 -0.08484305768062228 1107 7 -0.016418460126715217 1107 8 0.034948314979004635 1107 9 0.06805821065123752 1107 10-11 0.08930235231408545 1107 12-13 0.0986134051964811 1107 14-15 0.13875911993419265 1107 16-17 0.147044341028419 1107 18-19 0.10406986464090551 1107 20-21 0.09635934982127736 1107 22-23 0.13665855162311402 1107 24-25 0.10780374164829709 1107 26-27 0.05240554356706184 1107 28-29 -6.46552358269048E-4 1107 30-31 -0.07650388457806656 1107 32-33 -0.0729050156016271 1107 34-35 0.15281435455353432 1107 36-37 0.1568206829372656 1107 38-39 0.04309153746049077 1107 40-41 0.00813303653225006 1107 42-43 0.020611252626252963 1107 44-45 0.252578485732208 1107 46-47 0.32353494284292594 1107 48-49 0.28230365486432163 1107 50-51 0.27972529681689196 1107 52-53 0.17994938144139638 1107 54-55 0.19667023488698732 1107 56-57 0.2654676188165226 1107 58-59 0.34342451139958285 1107 60-61 0.2956462231077097 1107 62-63 0.27740296607075976 1107 64-65 0.27616591102079724 1107 66-67 0.07633497228243868 1107 68-69 0.20754595827261468 1107 70-71 0.3035261417844488 1107 72-73 0.23486818268565202 1107 74-75 0.2514833990472134 1107 76-77 0.24627492500136583 1107 78-79 0.2212591042180989 1107 80-81 0.16516811191031167 1107 82-83 0.07911473671374125 1107 84-85 0.15488360543501045 1107 86-87 0.12234051493556564 1107 88-89 0.18748688573309025 1107 90-91 0.34733048079538165 1107 92-93 0.4766600313269187 1107 94-95 0.49875434584554057 1107 96-97 0.5873327257480625 1107 98-99 0.6719198270046824 1107 100-101 1.1534691224565385 1108 1 0.11255984935834462 1108 2 0.2263652514701029 1108 3 0.05529376173764433 1108 4 0.1633633910942649 1108 5 0.22228003188285328 1108 6 0.18355980446599318 1108 7 0.12433540406516386 1108 8 0.12077001331192605 1108 9 0.17470028804691395 1108 10-11 0.27697283776202397 1108 12-13 0.29634747824873386 1108 14-15 0.4603830101862201 1108 16-17 0.51446725590759 1108 18-19 0.44663327700926914 1108 20-21 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50-51 -2.398017884898671 2316 52-53 -2.6432571555496978 2316 54-55 -2.5043792782294574 2316 56-57 -2.1931337762475955 2316 58-59 -2.0033924270153847 2316 60-61 -1.8479518454760857 2316 62-63 -2.025873124080288 2316 64-65 -2.1873330404520317 2316 66-67 -2.0601933968219868 2316 68-69 -2.1259921365000736 2316 70-71 -2.0943576994658493 2316 72-73 -2.117349320830872 2316 74-75 -2.0936418640848125 2316 76-77 -2.2065628570707325 2316 78-79 -2.101190329851576 2316 80-81 -2.111016775237985 2316 82-83 -2.0487595323322125 2316 84-85 -2.027523221680031 2316 86-87 -2.148459596461514 2316 88-89 -2.0634304683775966 2316 90-91 -2.437516865479221 2316 92-93 -2.216451010815927 2316 94-95 -2.1654884054851955 2316 96-97 -2.405017226841018 2316 98-99 -2.643391641865005 2316 100-101 -2.7263141576359615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24011.0 3 6137.0 4 5842.0 5 6672.0 6 7779.0 7 9358.0 8 11675.0 9 13596.0 10 16143.0 11 20051.0 12 24455.0 13 30045.0 14 31978.0 15 35806.0 16 43576.0 17 53084.0 18 47376.0 19 36068.0 20 36572.0 21 39109.0 22 41838.0 23 46633.0 24 52350.0 25 59057.0 26 68540.0 27 78742.0 28 92194.0 29 107059.0 30 127799.0 31 153728.0 32 187702.0 33 237034.0 34 313032.0 35 436505.0 36 619244.0 37 861950.0 38 738827.0 39 110537.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.93968337507461 18.677400690495027 14.246191610294504 22.136724324135866 2 15.286167087830876 20.99269682603189 39.62916128550788 24.091974800629362 3 20.24584770928584 25.25265008618832 29.599867622697317 24.901634581828517 4 12.434035893263404 16.42043729043693 37.86062468581734 33.28490213048232 5 13.363078389244649 38.100259287326 33.511979306801074 15.024683016628284 6 30.90415420858902 36.178817366154156 17.584090598520643 15.332937826736176 7 28.274588418394327 33.53446410526266 20.57977526877772 17.6111722075653 8 26.34954132856211 33.00050539335204 19.90224912489772 20.747704153188128 9 26.802586066810598 17.493342486378214 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34-35 22.9179116397794 26.151539113913557 29.128455468960468 21.802093777346567 36-37 22.71864297040142 25.76269643889026 29.657750472766548 21.860910117941774 38-39 22.09966759739949 26.392159864865945 29.711168825017413 21.79700371271715 40-41 22.035065458949862 26.4796183512454 29.67354370255154 21.811772487253194 42-43 21.72784176464643 26.795790802322866 28.64263024270993 22.833737190320768 44-45 22.088896077178127 26.310655215680363 28.67788766926889 22.922561037872626 46-47 22.678257834400462 26.874742567103958 28.24252736904489 22.204472229450687 48-49 22.741264705155032 26.688753708563677 28.21369888405924 22.356282702222053 50-51 21.85100868583364 26.98095125888588 28.781971882260315 22.38606817302016 52-53 22.40996673605789 27.473666089116445 27.800643253181747 22.315723921643922 54-55 21.731992345861986 26.653201245880403 28.291637244389804 23.323169163867806 56-57 22.482293613839424 26.950787313451823 27.80122564576675 22.765693426942 58-59 23.19057079401763 26.398335480060357 27.787813374725495 22.623280351196513 60-61 22.45260665352 26.68038080558957 28.952680822810965 21.914331718079463 62-63 22.05610577409001 26.748556968315786 28.713606344633785 22.48173091296042 64-65 21.679914501716357 27.301561736088992 28.329203721897816 22.689320040296835 66-67 22.045577497936733 26.942875473782724 27.658166063637406 23.353380964643137 68-69 21.68060046698184 26.873954809094048 28.333314627455824 23.112130096468288 70-71 22.444175034236874 26.85049029032079 27.853102248378985 22.852232427063353 72-73 22.280495096481552 26.34645946827235 28.498338130272383 22.87470730497372 74-75 21.65043373540296 27.71032283505066 27.77358165120265 22.86566177834373 76-77 22.093267760521133 26.747877052847148 27.81471656533549 23.34413862129623 78-79 22.139854736035247 26.787325288040392 28.139455898894784 22.933364077029577 80-81 22.410306659197353 27.233084735847036 27.654464694444474 22.70214391051114 82-83 22.360020673230505 26.788787629305084 28.017972205412956 22.833219492051455 84-85 22.044688572691236 26.843706971582726 27.834740958409526 23.276863497316512 86-87 22.169613211056596 26.82052958537499 27.94723779837469 23.06261940519372 88-89 22.084802037743934 26.862307149920934 27.478469535289783 23.574421277045346 90-91 22.164474742474365 26.465951916910136 27.921980217608798 23.447593123006705 92-93 22.579927505502866 26.65671684906325 27.908230441599617 22.855125203834273 94-95 22.217402645178787 26.350621805110574 27.968136060331776 23.46383948937886 96-97 21.752524941287263 26.235155022514995 28.48288198560508 23.529438050592667 98-99 22.1628761588477 26.543856409452783 27.44762589824888 23.845641533450642 100-101 22.207143193300602 25.565693007893653 28.297049643642364 23.93011415516338 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6927.0 1 4543.0 2 2260.0 3 2413.0 4 2159.5 5 2155.5 6 3407.0 7 6345.5 8 9692.5 9 10769.5 10 9154.0 11 7441.0 12 7156.5 13 7400.5 14 7750.0 15 8056.0 16 8423.0 17 8733.0 18 8891.5 19 9169.5 20 9946.0 21 11281.5 22 12956.0 23 15241.5 24 17796.5 25 21107.5 26 26455.0 27 32396.5 28 37159.5 29 43244.0 30 51269.5 31 59978.5 32 71077.0 33 81704.5 34 91790.0 35 100405.5 36 110846.0 37 121818.0 38 126194.0 39 127614.5 40 128245.0 41 134649.0 42 143104.0 43 148081.5 44 151406.5 45 155238.5 46 158714.5 47 182740.0 48 251676.0 49 254159.0 50 190507.5 51 161750.0 52 151793.5 53 151234.0 54 151783.5 55 142543.0 56 127544.0 57 115121.5 58 106401.0 59 95908.0 60 84614.0 61 73507.5 62 61638.0 63 49763.5 64 39123.5 65 31046.0 66 22903.0 67 18700.5 68 13687.5 69 7834.5 70 5738.5 71 4686.5 72 3961.0 73 3293.0 74 2553.0 75 2166.5 76 1600.5 77 918.5 78 549.0 79 398.5 80 289.0 81 207.0 82 161.5 83 128.0 84 88.0 85 65.0 86 52.0 87 46.0 88 35.5 89 20.5 90 17.5 91 17.0 92 12.5 93 11.0 94 11.0 95 7.5 96 4.5 97 4.5 98 3.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2806627465215162 2 0.16978005990727907 3 0.19773872164968934 4 0.16781405482546635 5 0.24032860015885318 6 0.19922874655380002 7 0.24599897271060778 8 0.25094502760064186 9 0.1554178754148788 10-11 0.2109523452785043 12-13 0.2172849511209747 14-15 0.17815110259773426 16-17 0.28973741208335696 18-19 0.23699673891493905 20-21 0.20399889572598773 22-23 0.25108989113298597 24-25 0.23999748351349529 26-27 0.3505800956678768 28-29 0.3212866199488673 30-31 0.2958630700224787 32-33 0.19693162482662938 34-35 0.3678085086216566 36-37 0.4036518854816526 38-39 0.3837538445746745 40-41 0.3458409886811914 42-43 0.37959419171736547 44-45 0.3880169713836578 46-47 0.39292163669302216 48-49 0.41308870987435364 50-51 0.40530746870844225 52-53 0.4049039202969123 54-55 0.39966813834219006 56-57 0.37795930328091065 58-59 0.3528875647902148 60-61 0.31974485806891945 62-63 0.3878928026416486 64-65 0.420963077596772 66-67 0.42616781736599196 68-69 0.4306999764493286 70-71 0.43047233375564503 72-73 0.42186330097633884 74-75 0.4171345413848209 76-77 0.40079600441544044 78-79 0.3753828018842193 80-81 0.38623721941485895 82-83 0.3962328031466015 84-85 0.39694677341315454 86-87 0.3922387086119714 88-89 0.4006614882782638 90-91 0.42175982702466447 92-93 0.4205284869997396 94-95 0.429189256754883 96-97 0.43013086971511966 98-99 0.42671622930986597 100-101 0.40028898205223606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4832134.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.38948593975868 #Duplication Level Percentage of deduplicated Percentage of total 1 67.08526505983997 23.07027779540351 2 14.30190679149305 9.836704450355787 3 6.419474138409191 6.622872468704019 4 3.458395359824989 4.757297544033124 5 2.1438009348436418 3.686210605322346 6 1.3989295470070238 2.886508079250661 7 0.9790700774249791 2.356880166315133 8 0.684750471493414 1.8838573369332707 9 0.5286180670132905 1.636101322475037 >10 2.6193708518630414 15.838897512145685 >50 0.19727639932205715 4.725949487171167 >100 0.1614597098032368 10.912707601699337 >500 0.01453581585070146 3.365353604255021 >1k 0.006117155567350688 4.001512269936271 >5k 5.450930703579797E-4 1.3009981361397294 >10k+ 4.8452717365153754E-4 3.117871619859767 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34348 0.7108246584221382 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24603 0.5091539266088234 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20010 0.4141027546007623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18611 0.38515074292227824 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15477 0.3202932700127935 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13965 0.28900274702646905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12468 0.2580226458951676 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10212 0.2113351988996994 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8769 0.181472616446481 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7926 0.16402690819418503 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7665 0.15862556791678376 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7032 0.14552576563481062 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7006 0.14498770108610398 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6714 0.13894482230832175 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6272 0.12979772498030892 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6051 0.12522417631630248 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5028 0.10405340580372976 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4873 0.10084571330182482 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.069479033487068E-5 0.0 9 0.0 0.0 0.0 2.069479033487068E-5 0.0 10-11 0.0 0.0 0.0 6.208437100461204E-5 0.0 12-13 0.0 0.0 0.0 1.345161371766594E-4 0.0 14-15 0.0 0.0 0.0 2.1729529851614214E-4 0.0 16-17 0.0 0.0 0.0 3.5181143569280155E-4 0.0 18-19 0.0 0.0 0.0 4.966749680368963E-4 0.0 20-21 0.0 0.0 0.0 6.829280810507325E-4 0.0 22-23 0.0 0.0 0.0 0.001210645234589935 0.0 24-25 0.0 0.0 0.0 0.002162605589993986 0.0 26-27 0.0 0.0 0.0 0.003259429477742132 0.0 28-29 0.0 0.0 0.0 0.007098313084860643 0.0 30-31 0.0 0.0 0.0 0.01755952959913777 0.0 32-33 0.0 0.0 0.0 0.03829570951467819 0.0 34-35 0.0 0.0 0.0 0.06992769654152803 0.0 36-37 0.0 0.0 0.0 0.12161293540286755 0.0 38-39 0.0 0.0 0.0 0.19262710843697628 0.0 40-41 0.0 0.0 0.0 0.274454309421055 0.0 42-43 0.0 1.034739516743534E-5 0.0 0.3594478133263689 0.0 44-45 0.0 2.069479033487068E-5 0.0 0.4655706981635857 0.0 46-47 0.0 2.069479033487068E-5 0.0 0.5962272569427918 0.0 48-49 0.0 2.069479033487068E-5 0.0 0.7338165704841795 0.0 50-51 0.0 2.069479033487068E-5 0.0 0.8833260832584526 0.0 52-53 0.0 2.069479033487068E-5 0.0 1.0340565886624833 0.0 54-55 0.0 2.069479033487068E-5 0.0 1.19779376979198 0.0 56-57 0.0 2.069479033487068E-5 0.0 1.4014201592919402 0.0 58-59 0.0 2.069479033487068E-5 0.0 1.6206918102850625 0.0 60-61 0.0 2.069479033487068E-5 0.0 1.8661009814711265 0.0 62-63 0.0 2.069479033487068E-5 0.0 2.1071538992917 0.0 64-65 0.0 2.069479033487068E-5 0.0 2.351186866920495 0.0 66-67 1.034739516743534E-5 2.069479033487068E-5 0.0 2.6374785964130965 0.0 68-69 2.069479033487068E-5 2.069479033487068E-5 0.0 2.9635457129293186 0.0 70-71 2.069479033487068E-5 2.069479033487068E-5 0.0 3.325683848999221 0.0 72-73 2.069479033487068E-5 2.069479033487068E-5 0.0 3.6839520592765016 0.0 74-75 2.069479033487068E-5 2.069479033487068E-5 0.0 4.056189253029821 0.0 76-77 2.069479033487068E-5 2.069479033487068E-5 0.0 4.441960839662145 0.0 78-79 2.069479033487068E-5 2.069479033487068E-5 0.0 4.895218137576483 0.0 80-81 2.069479033487068E-5 2.069479033487068E-5 0.0 5.3942626591067215 0.0 82-83 2.069479033487068E-5 2.069479033487068E-5 0.0 5.880466063234174 0.0 84-85 2.069479033487068E-5 2.069479033487068E-5 0.0 6.38884393520544 0.0 86-87 2.069479033487068E-5 2.069479033487068E-5 0.0 6.906079177440029 0.0 88-89 2.069479033487068E-5 2.069479033487068E-5 0.0 7.462417226012358 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 27105 0.0 26.147757 1 TACATGG 27685 0.0 25.292976 2 ACATGGG 27670 0.0 24.346544 3 GTAAGCG 500 0.0 22.807169 94-95 GAGTACT 19825 0.0 22.146738 12-13 CCGTCGT 280 6.730261E-11 22.023653 9 AGTACTT 20315 0.0 21.320498 12-13 CATGGGA 17470 0.0 21.127338 4 GTACTTT 21230 0.0 20.636675 14-15 ACCGTCG 350 3.6379788E-12 20.331001 8 CATGGGG 9950 0.0 20.216263 4 GTATAGG 1625 0.0 19.891233 1 TACCTGG 3335 0.0 19.204193 2 ATGGGAG 6800 0.0 19.046354 5 ACTTGAC 2245 0.0 18.595245 8 ACTTTTT 24225 0.0 18.517242 16-17 GCGTATC 130 0.0075352 18.282385 1 TACTTTT 24000 0.0 17.99775 14-15 TCGCGCT 1260 0.0 17.917404 60-61 CCAAGAC 2995 0.0 17.58265 3 >>END_MODULE