FastQCFastQC Report
Thu 26 May 2016
SRR938818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4832134
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT387130.8011574182338487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234500.4852928333527175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198390.4105639454534994No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT180640.37383069260910395No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT143750.29748761106376603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134710.27877952060104294No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC128760.26646612035179484No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT109850.22733227182855442No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC95200.19701440398796885No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC91760.18989539611277337No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT86170.17832700831558065No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG79410.16433733004920806No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA72140.14929221747575708No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA69750.144346162585723No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC67240.13915177021167044No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA62450.12923896564126738No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA59720.1235892878798477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52690.10904085027443361No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG267550.024.6465971
TACATGG272700.023.7747212
ACATGGG272600.022.7387833
GAGTACT208100.021.38502912-13
AGTACTT210500.020.7918612-13
GTACTTT218050.020.40919314-15
CATGGGG94000.020.1967754
CATGGGA173650.019.351224
GTAAGCG3750.018.97750594-95
TGCACCG15750.018.382275
GTGTAAG18700.018.0640621
GCACCGT17900.018.0233276
ACTTTTT248800.017.93405716-17
TACTTTT246150.017.63601114-15
ATGGGCG7000.017.6288995
GAGTGCA31400.017.0730029
GATGTGC29100.016.9551458
ATGGGAT66800.016.9102635
GTGTAGC21500.016.596691
TCGCGCT13800.016.50311760-61
Produced by FastQC (version 0.11.5)